Leptospira species categorized by arbitrarily primed polymerase chain reaction (PCR) and by mapped restriction polymorphisms in PCR-amplified rRNA genes.

Abstract:

:Reference strains from 48 selected serovars representing eight species of Leptospira were examined by two polymerase chain reaction (PCR)-based strategies. First, mapped restriction site polymorphisms (MRSP) were examined in PCR products from portions of rrs (16S rRNA gene) and rrl (23S rRNA gene). Twenty MRSP and 2 length polymorphisms were used to group reference strains into 16 MRSP profiles. Species assignments were consistent with those obtained by a second method, genomic fingerprinting with arbitrarily primed PCR, in which strains within a species were characterized by many shared arbitrarily primed PCR products. The results of both of these methods were in general agreement with those of previous studies that used DNA-DNA relatedness and confirmed the high level of divergence among the recognized species of Leptospira. However, Leptospira meyeri serovar ranarum and evansi strains were indistinguishable from some strains of Leptospira interrogans sensu stricto. Intervening sequences of about 485 to 740 bp were located near base 1230 in rrl of some strains.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ralph D,McClelland M,Welsh J,Baranton G,Perolat P

doi

10.1128/jb.175.4.973-981.1993

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

973-81

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

175

pub_type

杂志文章
  • Hydroxyethylthiazole uptake in Escherichia coli: general properties and relationship between uptake and thiamine biosynthesis.

    abstract::Uptake of (35)S-hydroxyethylthiazole (4-methyl-5-hydroxyethylthiazole) by Escherichia coli intact cells was studied. Hydroxyethylthiazole was taken up in the presence and absence of glucose at the same rate. The uptake was almost proportional to a hydroxyethylthiazole concentration gradient up to 0.1 mM with no tenden...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.3.1280-1286.1973

    authors: Yamasaki H,Sanemori H,Yamada K,Kawasaki T

    更新日期:1973-12-01 00:00:00

  • Novel class of mutations of pilS mutants, encoding plasmid R64 type IV prepilin: interface of PilS-PilV interactions.

    abstract::The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01204-07

    authors: Shimoda E,Muto T,Horiuchi T,Furuya N,Komano T

    更新日期:2008-02-01 00:00:00

  • Development and use of a polyvalent conjugate to differentiate Histoplasma capsulatum and Histoplasma duboisii from other pathogens.

    abstract::Previous investigations have demonstrated the existence of five Histoplasma capsulatum serotypes. Available specific fluorescent-antibody reagents stain only four of the five serotypes. Antibodies produced against the most complete H. capsulatum serotype were labeled with fluorescein isothiocyanate to develop a reagen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.4.1243-1246.1968

    authors: Kaufman L,Blumer S

    更新日期:1968-04-01 00:00:00

  • Autotrophic and heterotrophic metabolism of Hydrogenomonas. I. Growth yields and patterns under dual substrate conditions.

    abstract::Auxotrophic mutants of Hydrogenomonas eutropha and H. facilis requiring utilizable amino acids were employed to demonstrate the simultaneous utilization of H(2) and an organic substrate for growth. The ratio of the cell yields under dual substrate conditions compared to heterotrophic conditions indicated the relative ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.4.1469-1475.1968

    authors: DeCicco BT,Stukus PE

    更新日期:1968-04-01 00:00:00

  • Cloning and characterization of the gene cluster for palatinose metabolism from the phytopathogenic bacterium Erwinia rhapontici.

    abstract::Erwinia rhapontici is able to convert sucrose into isomaltulose (palatinose, 6-O-alpha-D-glucopyranosyl-D-fructose) and trehalulose (1-O-alpha-D-glucopyranosyl-D-fructose) by the activity of a sucrose isomerase. These sucrose isomers cannot be metabolized by plant cells and most other organisms and therefore are possi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.8.2425-2430.2001

    authors: Börnke F,Hajirezaei M,Sonnewald U

    更新日期:2001-04-01 00:00:00

  • Identification of methylation sites in Thermotoga maritima chemotaxis receptors.

    abstract::Adaptation in bacterial chemotaxis involves reversible methylation of specific glutamate residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins. The specific sites of methylation in Salmonella enterica and Escherichia coli chemoreceptors, identified 2 decades ago, established a consensus seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00181-06

    authors: Perez E,Zheng H,Stock AM

    更新日期:2006-06-01 00:00:00

  • A combination of sbmA and tolC mutations in Escherichia coli K-12 Tn10-carrying strains results in hypersusceptibility to tetracycline.

    abstract::Previously, we demonstrated that Escherichia coli tolC mutations reduce the high-level resistance to tetracycline afforded by the transposon Tn10-encoded TetA pump from resistance at 200 microg/ml to resistance at 40 microg/ml. In this study, we found that the addition of an sbmA mutation to a tolC::Tn10 mutant exacer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01844-07

    authors: de Cristóbal RE,Vincent PA,Salomón RA

    更新日期:2008-02-01 00:00:00

  • Chloramphenicol and the stimulation of ribonucleic acid synthesis in Escherichia coli.

    abstract::Data have been obtained which imply that chloramphenicol stimulation of ribonucleic acid (RNA) synthesis is a result of the accumulation of aminoacyl transfer RNA (tRNA) molecules. The data also support the hypothesis that chloramphenicol exerts an additional effect upon the stimulation of RNA synthesis. This effect m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.587-592.1969

    authors: Kaplan S

    更新日期:1969-05-01 00:00:00

  • Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine.

    abstract::The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic path...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.2.764-774.1975

    authors: Kelln RA,Kinahan JJ,Foltermann KF,O'Donovan GA

    更新日期:1975-11-01 00:00:00

  • Overproduction of the AlgT Sigma Factor Is Lethal to Mucoid Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa isolates from chronic lung infections often overproduce alginate, giving rise to the mucoid phenotype. Isolation of mucoid strains from chronic lung infections correlates with a poor patient outcome. The most common mutation that causes the mucoid phenotype is called mucA22 and results in a trun...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00445-20

    authors: Cross AR,Raghuram V,Wang Z,Dey D,Goldberg JB

    更新日期:2020-09-23 00:00:00

  • Sll1717 affects the redox state of the plastoquinone pool by modulating quinol oxidase activity in thylakoids.

    abstract::A Synechocystis sp. strain PCC 6803 mutant lacking CtaI, a main subunit of cytochrome c oxidase, is not capable of growing at light intensities below 5 micromol photons m(-2) s(-1), presumably due to an overreduced plastoquinone pool in the thylakoid membrane. Upon selection for growth at light intensities below 5 mic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.4.1286-1294.2006

    authors: Kufryk GI,Vermaas WF

    更新日期:2006-02-01 00:00:00

  • Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824.

    abstract::A gene (aad) coding for an aldehyde/alcohol dehydrogenase (AAD) was identified immediately upstream of the previously cloned ctfA (J. W. Cary, D. J. Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.3.871-885.1994

    authors: Nair RV,Bennett GN,Papoutsakis ET

    更新日期:1994-02-01 00:00:00

  • An AlgU-Regulated Antisense Transcript Encoded within the Pseudomonas syringae fleQ Gene Has a Positive Effect on Motility.

    abstract::Production of bacterial flagella is controlled by a multitiered regulatory system that coordinates the expression of 40 to 50 subunits and ordered assembly of these elaborate structures. Flagellar expression is environmentally controlled, presumably to optimize the benefits and liabilities of having these organelles o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00576-17

    authors: Markel E,Dalenberg H,Monteil CL,Vinatzer BA,Swingle B

    更新日期:2018-03-12 00:00:00

  • Control of Escherichia coli growth by CO2.

    abstract::Escherichia coli B dependence on CO2 for growth was demonstrated. At suboptimal CO2 concentrations the rate of growth was controlled by CO2 concentration. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.1162-1164.1978

    authors: Repaske R,Clayton MA

    更新日期:1978-09-01 00:00:00

  • The phosphoenolpyruvate phosphotransferase system regulates Vibrio cholerae biofilm formation through multiple independent pathways.

    abstract::The bacterial phosphoenolpyruvate phosphotransferase system (PTS) is a highly conserved phosphotransfer cascade that participates in the transport and phosphorylation of selected carbohydrates and modulates many cellular functions in response to carbohydrate availability. It plays a role in the virulence of many bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00213-10

    authors: Houot L,Chang S,Pickering BS,Absalon C,Watnick PI

    更新日期:2010-06-01 00:00:00

  • Bacillus subtilis ccpA gene mutants specifically defective in activation of acetoin biosynthesis.

    abstract::A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.19.5611-5614.2000

    authors: Turinsky AJ,Moir-Blais TR,Grundy FJ,Henkin TM

    更新日期:2000-10-01 00:00:00

  • Regulation of Biofilm Aging and Dispersal in Bacillus subtilis by the Alternative Sigma Factor SigB.

    abstract::Bacterial biofilms are important in natural settings, biotechnology, and medicine. However, regulation of biofilm development and its persistence in different niches is complex and only partially understood. One key step during the biofilm life cycle is dispersal, when motile cells abandon the mature biofilm to spread...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00473-18

    authors: Bartolini M,Cogliati S,Vileta D,Bauman C,Rateni L,Leñini C,Argañaraz F,Francisco M,Villalba JM,Steil L,Völker U,Grau R

    更新日期:2018-12-20 00:00:00

  • Physiologic and proteomic evidence for a role of nitric oxide in biofilm formation by Nitrosomonas europaea and other ammonia oxidizers.

    abstract::NO, a free radical gas, is the signal for Nitrosomonas europaea cells to switch between different growth modes. At an NO concentration of more than 30 ppm, biofilm formation by N. europaea was induced. NO concentrations below 5 ppm led to a reversal of the biofilm formation, and the numbers of motile and planktonic (m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.9.2781-2788.2004

    authors: Schmidt I,Steenbakkers PJ,op den Camp HJ,Schmidt K,Jetten MS

    更新日期:2004-05-01 00:00:00

  • Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes.

    abstract::The DNA-binding factor BifA (previously called VF1) binds upstream of the developmentally regulated site-specific recombinase gene xisA in the cyanobacterium Anabaena sp. strain PCC 7120. Besides binding xisA, BifA also binds the glnA, rbcL, and nifH promoter regions. DNase I footprint analysis of BifA binding to glnA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1214-1223.1994

    authors: Ramasubramanian TS,Wei TF,Golden JW

    更新日期:1994-03-01 00:00:00

  • Carrier-mediated transport of folate in a mutant of Pediococcus cerevisiae.

    abstract::A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.485-490.1973

    authors: Mandelbaum-Shavit F,Grossowicz N

    更新日期:1973-05-01 00:00:00

  • Activation of inactive nitrogenase by acid-treated component I.

    abstract::When Azotobacter vinelandii was derepressed for nitrogenase synthesis in a N-free medium containing tungstate instead of molybdate, an inactive component I was synthesized. Although this inactive component I could be activated in vivo upon addition of molybdate to the medium, it could not be activated in vitro when mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.2.697-701.1974

    authors: Nagatani HH,Shah VK,Brill WJ

    更新日期:1974-11-01 00:00:00

  • Active transport of manganese in isolated membrane vesicles of Bacillus subtilis.

    abstract::Membrane vesicles isolated from cells of bacillus subtilis W23 accumulate manganese in the presence of an energy source. The artificial electron donor system ascorbate and phenazine methosulfate or reduced nicotinamide adenine dinucleotide and phenazine methosulfate can supply the energy for the uptake. D-Lactate in t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.123-127.1975

    authors: Bhattacharyya P

    更新日期:1975-07-01 00:00:00

  • Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al.

    abstract::Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.16.5077-5085.1994

    authors: Goldman BS,Lin JT,Stewart V

    更新日期:1994-08-01 00:00:00

  • Resistance of Escherichia coli to penicillins. VI. Purification and characterization of the chromosomally mediated penicillinase present in ampA-containing strains.

    abstract::The chromosomally mediated penicillinase present in three strains of Escherichia coli K-12 has been purified and characterized. Two of the strains carried the ampA gene and the third the wild-type allele. The purification involves release of the enzyme by spheroplast formation, dialysis, chromatography on sulfoethyl c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.1.218-231.1970

    authors: Linström EB,Boman HG,Steele BB

    更新日期:1970-01-01 00:00:00

  • Molecular cloning and functional expression of alternative oxidase from Candida albicans.

    abstract::The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.13.4098-4102.1999

    authors: Huh WK,Kang SO

    更新日期:1999-07-01 00:00:00

  • A subassembly of R27-encoded transfer proteins is dependent on TrhC nucleoside triphosphate-binding motifs for function but not formation.

    abstract::The transfer of plasmid DNA molecules between bacterial cells is achieved by a large array of conjugative transfer proteins which assemble into both cytoplasmic and membrane-associated complexes. TrhC is a membrane-associated protein that is required for the transfer of the IncHI1 resistance plasmid R27. Homologous pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.6.1606-1613.2004

    authors: Gilmour MW,Taylor DE

    更新日期:2004-03-01 00:00:00

  • Analysis of plasmid deletional instability in Bacillus subtilis.

    abstract::Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination eve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.3.1014-1023.1985

    authors: Hahn J,Dubnau D

    更新日期:1985-06-01 00:00:00

  • In Escherichia coli, MreB and FtsZ direct the synthesis of lateral cell wall via independent pathways that require PBP 2.

    abstract::In Escherichia coli, the cytoplasmic proteins MreB and FtsZ play crucial roles in ensuring that new muropeptide subunits are inserted into the cell wall in a spatially correct way during elongation and division. In particular, to retain a constant diameter and overall shape, new material must be inserted into the wall...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01812-08

    authors: Varma A,Young KD

    更新日期:2009-06-01 00:00:00

  • Phage-associated mutator phenotype in group A streptococcus.

    abstract::Defects in DNA mismatch repair (MMR) occur frequently in natural populations of pathogenic and commensal bacteria, resulting in a mutator phenotype. We identified a unique genetic element in Streptococcus pyogenes strain SF370 that controls MMR via a dynamic process of prophage excision and reintegration in response t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01569-07

    authors: Scott J,Thompson-Mayberry P,Lahmamsi S,King CJ,McShan WM

    更新日期:2008-10-01 00:00:00

  • Phospholipid turnover in a conditional polyamine auxotroph of Escherichia coli.

    abstract::Polyamines are required for rapid phospholipid turnover in Escherichia coli growing in media of low osmolarity. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.3.1479-1481.1973

    authors: Munro GF,Bell CA

    更新日期:1973-12-01 00:00:00