Identification of methylation sites in Thermotoga maritima chemotaxis receptors.

Abstract:

:Adaptation in bacterial chemotaxis involves reversible methylation of specific glutamate residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins. The specific sites of methylation in Salmonella enterica and Escherichia coli chemoreceptors, identified 2 decades ago, established a consensus sequence for methylation by methyltransferase CheR. Here we report the in vitro methylation of chemoreceptors from Thermotoga maritima, a hyperthermophile that has served as a useful source of chemotaxis proteins for structural analysis. Sites of methylation have been identified by liquid chromatography-mass spectrometry/mass spectrometry. Fifteen sites of methylation were identified within the cytoplasmic domains of four different T. maritima chemoreceptors. The results establish a consensus sequence for chemoreceptor methylation sites in T. maritima that is distinct from the previously identified consensus sequence for E. coli and S. enterica. These findings suggest that consensus sequences for posttranslational modifications in one organism may not be directly extrapolated to analogous modifications in other bacteria.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Perez E,Zheng H,Stock AM

doi

10.1128/JB.00181-06

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

4093-100

issue

11

eissn

0021-9193

issn

1098-5530

pii

188/11/4093

journal_volume

188

pub_type

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