Molecular and catalytic properties of ribulose 1,5-bisphosphate carboxylase from the photosynthetic extreme halophile Ectothiorhodospira halophila.

abstract：:Mycobacterium tuberculosis utilizes the methylerythritol phosphate (MEP) pathway for biosynthesis of isopentenyl diphosphate and its isomer, dimethylallyl diphosphate, precursors of all isoprenoid compounds. This pathway is of interest as a source of new drug targets, as it is absent from humans and disruption of the ...

journal_title：Journal of bacteriology

authors： Eoh H,Brown AC,Buetow L,Hunter WN,Parish T,Kaur D,Brennan PJ,Crick DC

更新日期：2007-12-01 00:00:00

abstract：:Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

journal_title：Journal of bacteriology

authors： Romeo T,Preiss J

更新日期：1989-05-01 00:00:00

abstract：:The binding of iron-loaded human transferrin at the surface of Neisseria meningitidis is mediated by two polypeptides, Tbp1 and Tbp2. Predicted Tbp amino acid sequences from N. meningitidis strains are highly divergent. This variability is particularly pronounced throughout the Tbp2 polypeptide. In this study, a highl...

journal_title：Journal of bacteriology

authors： Vonder Haar RA,Legrain M,Kolbe HV,Jacobs E

更新日期：1994-10-01 00:00:00

abstract：:Benzoyl coenzyme A (benzoyl-CoA) reductase is a key enzyme in the anaerobic metabolism of aromatic compounds catalyzing the ATP-driven reductive dearomatization of benzoyl-CoA. The enzyme from Thauera aromatica uses a reduced 2[4Fe-4S] ferredoxin as electron donor. In this work, we identified 2-oxoglutarate:ferredoxin...

journal_title：Journal of bacteriology

authors： Dörner E,Boll M

更新日期：2002-07-01 00:00:00

abstract：:The effects of surfaces on the physiology of bacteria adhering to surfaces or immobilized within biofilms are receiving more interest. A study of the effects of hydrophobic and hydrophilic substrata on the colonization behavior of a marine bacterium, SW5, revealed major differences in the morphology of SW5 on these su...

journal_title：Journal of bacteriology

authors： Dalton HM,Poulsen LK,Halasz P,Angles ML,Goodman AE,Marshall KC

更新日期：1994-11-01 00:00:00

abstract：:The bacteriophage T4 MotA protein is a transcriptional activator of T4-modified host RNA polymerase and is required for activation of the middle class of T4 promoters. MotA alone binds to the -30 region of T4 middle promoters, a region that contains the MotA box consensus sequence [(t/a)(t/a)TGCTT(t/c)A]. We report th...

journal_title：Journal of bacteriology

authors： Gerber JS,Hinton DM

更新日期：1996-11-01 00:00:00

abstract：:Acyl-homoserine lactone (acyl-HSL) quorum-sensing signaling is common to many Proteobacteria. Acyl-HSLs are synthesized by the LuxI family of synthases, and the signal response is mediated by members of the LuxR family of transcriptional regulators. Burkholderia thailandensis is a member of a closely related cluster o...

journal_title：Journal of bacteriology

authors： Chandler JR,Duerkop BA,Hinz A,West TE,Herman JP,Churchill ME,Skerrett SJ,Greenberg EP

更新日期：2009-10-01 00:00:00

abstract：:The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterotetrameric (alphabetagammadelta), cytoplasmic hydrogenase (hydrogenase I) that catalyzes both H(2) production and the reduction of elemental sulfur to H(2)S. Herein is described the purification of a second enzyme of this type, hy...

journal_title：Journal of bacteriology

authors： Ma K,Weiss R,Adams MW

更新日期：2000-04-01 00:00:00

abstract：:We have determined the amounts of a number of small molecules and enzymes in the mother cell compartment and the developing forespore during sporulation of Bacillus megaterium. Significant amounts of adenosine 5'-triphosphate and reduced nicotinamide adenine dinucleotide were present in the forespore compartment befor...

journal_title：Journal of bacteriology

authors： Singh RP,Setlow B,Setlow P

更新日期：1977-06-01 00:00:00

abstract：:Several environmental stresses have been demonstrated to increase polysaccharide intercellular adhesin (PIA) synthesis and biofilm formation by the human pathogens Staphylococcus aureus and Staphylococcus epidermidis. In this study we characterized an adaptive response of S. aureus SA113 to nitrite-induced stress and ...

journal_title：Journal of bacteriology

authors： Schlag S,Nerz C,Birkenstock TA,Altenberend F,Götz F

更新日期：2007-11-01 00:00:00

abstract：:A mutant of yeast lacking proteinase C (carboxypeptidase Y) activity has been found by using a histochemical stain to screen mutagenized colonies. This defect segregates 2:2 in meiotic tetrads. Cell extracts lacked the esterolytic, amidase, and proteolytic activities associated with proteinase C. The absence of protei...

journal_title：Journal of bacteriology

authors： Wolf DH,Fink GR

更新日期：1975-09-01 00:00:00

abstract：:Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of informat...

journal_title：Journal of bacteriology

authors： Pérez-Martínez I,Zhao Y,Murillo J,Sundin GW,Ramos C

更新日期：2008-01-01 00:00:00

abstract：:Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for chromosomal transposon mutants that require pyridoxine (vitamin B6) or ...

journal_title：Journal of bacteriology

authors： Lam HM,Tancula E,Dempsey WB,Winkler ME

更新日期：1992-03-01 00:00:00

abstract：:DNA sequence analysis of the modABCD operon of Escherichia coli revealed the presence of four open reading frames. The first gene, modA, codes for a 257-amino-acid periplasmic binding protein enunciated by the presence of a signal peptide-like sequence. The second gene (modB) encodes a 229-amino-acid protein with a po...

journal_title：Journal of bacteriology

authors： Maupin-Furlow JA,Rosentel JK,Lee JH,Deppenmeier U,Gunsalus RP,Shanmugam KT

更新日期：1995-09-01 00:00:00

abstract：:Nonpiliated, phage phi 6-resistant mutants of Pseudomonas syringae pv. phaseolicola were generated by Tn5 transposon mutagenesis. A P. syringae pv. phaseolicola LR700 cosmid library was screened with Tn5-containing EcoRI fragments cloned from nonpiliated mutants. The cosmid clone pVK253 complemented the nonpiliated mu...

journal_title：Journal of bacteriology

authors： Roine E,Nunn DN,Paulin L,Romantschuk M

更新日期：1996-01-01 00:00:00

abstract：:We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen. In fact, the addi...

journal_title：Journal of bacteriology

authors： Stults LW,Moshiri F,Maier RJ

更新日期：1986-06-01 00:00:00

abstract：:Pulse labeling of deoxyribonucleic acid (DNA) and radioautography have been used to study the effect of growth rate on nuclear replication in Aspergillus nidulans. When conidia were germinated in media supporting a fast growth rate, the radioactive pulse labeled either all of the nuclei in a cell or none of them. At s...

journal_title：Journal of bacteriology

authors： Kessel M,Rosenberger RF

更新日期：1968-06-01 00:00:00

abstract：:The Salmonella enterica serovar Typhi ompS2 gene codes for a 362-amino-acid outer membrane protein that contains motifs common to the porin superfamily. It is expressed at very low levels compared to the major OmpC and OmpF porins, as observed for S. enterica serovar Typhi OmpS1, Escherichia coli OmpN, and Klebsiella ...

journal_title：Journal of bacteriology

authors： Fernández-Mora M,Puente JL,Calva E

更新日期：2004-05-01 00:00:00

abstract：:Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share ...

journal_title：Journal of bacteriology

authors： Sutcliffe J,Blumenthal R,Walter A,Foulds J

更新日期：1983-11-01 00:00:00

abstract：:Pleiotropic effects of two ribosomal mutations for cycloheximide resistance were studied in a double-resistant homocaryon. The results obtained indicated that the combination of the two ribosomal mutations results in: (i) morphological abnormalities which suggest a severe distortion in the extension of cell walls and ...

journal_title：Journal of bacteriology

authors： Vomvoyanni VE,Argyrakis MP

更新日期：1979-08-01 00:00:00

abstract：:Candida albicans is an opportunistic pathogenic yeast which frequently develops resistance to the antifungal agent fluconazole (FCZ) in patients undergoing long-term therapy. FCZ-resistant strains often display a reduced intracellular FCZ accumulation which correlates with the overexpression of the ATP-binding cassett...

journal_title：Journal of bacteriology

authors： Alarco AM,Raymond M

更新日期：1999-02-01 00:00:00

abstract：:The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...

journal_title：Journal of bacteriology

authors： Griffiths MW,Sundaram TK

更新日期：1973-12-01 00:00:00

abstract：:A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...

journal_title：Journal of bacteriology

authors： Schupp T,Toupet C,Cluzel B,Neff S,Hill S,Beck JJ,Ligon JM

更新日期：1995-07-01 00:00:00

abstract：:A gene suppressing the defects of Escherichia coli rdgB (recA-dependent growth) and recA(Ts) rdgB mutants was cloned. The cloned gene suppresses the hyper-Rec phenotype and the enhanced level of SOS functions in rdgB mutants at the nonpermissive temperature. We identified the cloned gene as purA. ...

journal_title：Journal of bacteriology

authors： Clyman J,Cunningham RP

更新日期：1991-02-01 00:00:00

abstract：:The competence-related phenotypes of mutations in each of the four open reading frames associated with the comE locus of Bacillus subtilis are described. comEA and comEC are required for transformability, whereas the products of comEB and of the overlapping comER, which is transcribed in the reverse direction, are dis...

journal_title：Journal of bacteriology

authors： Inamine GS,Dubnau D

更新日期：1995-06-01 00:00:00

abstract：:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

journal_title：Journal of bacteriology

authors： Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

更新日期：2010-11-01 00:00:00

abstract：:Lactococcus lactis subsp. lactis ML3 contains high pools of proline or betaine when grown under conditions of high osmotic strength. These pools are created by specific transport systems. A high-affinity uptake system for glycine betaine (betaine) with a Km of 1.5 microM is expressed constitutively. The activity of th...

journal_title：Journal of bacteriology

authors： Molenaar D,Hagting A,Alkema H,Driessen AJ,Konings WN

更新日期：1993-09-01 00:00:00

abstract：:Rhodopseudomonas capsulata required potassium (or rubidium or cesium as analogs of potassium) for growth. These cations were actively accumulated by the cells by a process following Michaelis-Menten saturation kinetics. The monovalent cation transport system had Km's of 0.2 mM K+, 0.5 mM Rb+, and 2.6 mM Cs+. The rates...

journal_title：Journal of bacteriology

authors： Jasper P

更新日期：1978-03-01 00:00:00

abstract：:The adherence of Ruminococcus albus 8 to crystalline cellulose was studied, and an affinity-based assay was also used to identify candidate cellulose-binding protein(s). Bacterial adherence in cellulose-binding assays was significantly increased by the inclusion of either ruminal fluid or micromolar concentrations of ...

journal_title：Journal of bacteriology

authors： Pegden RS,Larson MA,Grant RJ,Morrison M

更新日期：1998-11-01 00:00:00

abstract：:The phylum Bacteroidetes is large and diverse, with rapid gliding motility and the ability to digest macromolecules associated with many genera and species. Recently, a novel protein secretion system, the Por secretion system (PorSS), was identified in two members of the phylum, the gliding bacterium Flavobacterium jo...

journal_title：Journal of bacteriology

authors： McBride MJ,Zhu Y

更新日期：2013-01-01 00:00:00