Regulation and timing of deoxyribonucleic acid synthesis in hyphae of Aspergillus nidulans.

abstract：:The control of men gene expression during growth and sporulation of Bacillus subtilis was examined at the transcriptional level. Two different approaches were used. (i) Steady-state levels of men-specific mRNA were measured directly. (ii) A men'-lacZ gene fusion was constructed. In both cases, it was observed that men...

journal_title：Journal of bacteriology

authors： Miller P,Mueller J,Hill K,Taber H

更新日期：1988-06-01 00:00:00

abstract：:The polymerase chain reaction (PCR) has been used to amplify DNA fragments by using eucaryotic genomic DNA as a template. We show that bacterial genomic DNA can be used as a template for PCR amplification. We demonstrate that DNA fragments at least as large as 4,400 base pairs can be amplified with fidelity and that t...

journal_title：Journal of bacteriology

authors： Shyamala V,Ames GF

更新日期：1989-03-01 00:00:00

abstract：:Motility in mycobacteria was described for the first time in 1999. It was reported that Mycobacterium smegmatis and Mycobacterium avium could spread on the surface of solid growth medium by a sliding mechanism and that the presence of cell wall glycopeptidolipids was essential for motility. We recently reported that M...

journal_title：Journal of bacteriology

authors： Agustí G,Astola O,Rodríguez-Güell E,Julián E,Luquin M

更新日期：2008-10-01 00:00:00

abstract：:Mutants of Schizosaccharomyces pombe, which were resistant to concentrations of colcemid inhibitory to the wild-type parent, were not diminished in their permeability to colcemid or colchicine. They also did not modify colcemid to a detectable derivative or to a product that was incapable of binding to brain tubulin. ...

journal_title：Journal of bacteriology

authors： Lederberg S,Gourse RL,Sackett DL

更新日期：1977-01-01 00:00:00

abstract：:Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool fo...

journal_title：Journal of bacteriology

authors： Gregory ST,Cate JH,Dahlberg AE

更新日期：2001-07-01 00:00:00

abstract：:Flavin-based electron bifurcation has recently been characterized as an essential energy conservation mechanism that is utilized by hydrogenotrophic methanogenic Archaea to generate low-potential electrons in an ATP-independent manner. Electron bifurcation likely takes place at the flavin associated with the α subunit...

journal_title：Journal of bacteriology

authors： Costa KC,Lie TJ,Xia Q,Leigh JA

更新日期：2013-11-01 00:00:00

abstract：:The chromosomal location of two genetic loci involved in the transport of cyanocobalamin (B(12)) in Escherichia coli K-12 was determined. One gene, btuA, is believed to code for the transport protein in the cytoplasmic membrane, because a mutant with an alteration in this gene has lost the ability to accumulate B(12) ...

journal_title：Journal of bacteriology

authors： Kadner RJ,Liggins GL

更新日期：1973-08-01 00:00:00

abstract：:Ruthenium red stained a capsule external to the exine of the Azotobacter cyst. The central body is therefore surrounded by three layers, the intine, the exine, and the capsule, all containing acid mucopolysaccharide. Vesicles that appear to originate from the contracting cell membrane of the central body may account f...

journal_title：Journal of bacteriology

authors： Pope LM,Wyss O

更新日期：1970-04-01 00:00:00

abstract：:A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bac...

journal_title：Journal of bacteriology

authors： Cahn FH,Fox MS

更新日期：1968-03-01 00:00:00

abstract：:Highly efficient promoters of coliphage T5 were identified by selecting for functional properties. Eleven such promoters belonging to all three expression classes of the phage were analyzed. Their average AT content was 75% and reached 83% in subregions of the sequences. Besides the well-known conserved sequences arou...

journal_title：Journal of bacteriology

authors： Gentz R,Bujard H

更新日期：1985-10-01 00:00:00

abstract：:The enzyme nicotinamide mononucleotide (NMN) adenylyltransferase (EC 2.7.7.1) catalyzes the synthesis of NAD+ and nicotinic acid adenine dinucleotide. It has been purified to homogeneity from cellular extracts of the thermophilic archaeon Sulfolobus solfataricus. Through a database search, a highly significant match w...

journal_title：Journal of bacteriology

authors： Raffaelli N,Pisani FM,Lorenzi T,Emanuelli M,Amici A,Ruggieri S,Magni G

更新日期：1997-12-01 00:00:00

abstract：:Martin, Alice J. (Nassau County Department of Health, Hempstead, N.Y.), and Margaret O'Brien. Detection of enteropathogenic Escherichia coli in fecal cultures by use of a modified fluorescent-antibody technique. J. Bacteriol. 89:570-573. 1965.-The application of the fluorescent-antibody (FA) technique to cultures of f...

journal_title：Journal of bacteriology

authors： MARTIN AJ,O'BRIEN M

更新日期：1965-03-01 00:00:00

abstract：:Organic compounds exhibit various levels of toxicity toward living organisms based upon their ability to insert into biological membranes and disrupt normal membrane function. The primary mechanism responsible for organic solvent tolerance in many bacteria is energy-dependent extrusion via efflux pumps. One such bacte...

journal_title：Journal of bacteriology

authors： Sun X,Zahir Z,Lynch KH,Dennis JJ

更新日期：2011-06-01 00:00:00

abstract：:Clostridium perfringens enterotoxin was detected intracellularly about 3 hr after the inoculation of vegetative cells into sporulation medium. The subsequent increase in intracellular enterotoxin concentration roughly paralleled but followed by 2.5 to 5 hr the increase in number of heat-resistant spores. The increase ...

journal_title：Journal of bacteriology

authors： Duncan CL

更新日期：1973-02-01 00:00:00

abstract：:Azotobacter vinelandii produces two detectable catalases during growth on minimal medium. The heat-labile catalase expressed during exponential growth phase was identified as a KatG homologue by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a mixed protein sample. The second catalase was heat resista...

journal_title：Journal of bacteriology

authors： Sandercock JR,Page WJ

更新日期：2008-02-01 00:00:00

abstract：:For the pathogenic yeast Candida albicans, secreted aspartyl proteinase (Sap) activity has been correlated with virulence. A family consisting of at least eight SAP genes can be drawn upon to produce Sap enzymatic activity. In this study, the levels of Sap1, Sap2, and Sap3 isoenzymes were monitored under a variety of ...

journal_title：Journal of bacteriology

authors： White TC,Agabian N

更新日期：1995-09-01 00:00:00

abstract：:Tsukada, Yoji (Kyoto University, Kyoto, Japan), Tsunetake Sugimori, Kazutami Imai, and Hideo Katagiri. Action of cycloheximide on Zygosaccharomyces soja. J. Bacteriol. 83:70-75. 1962.-Cycloheximide is known to inhibit the growth of some species of Saccharomyces and other fungi. A new type of action of this antibiotic,...

journal_title：Journal of bacteriology

authors： TSUKADA Y,SUGIMORI T,IMAI K,KATAGIRI H

更新日期：1962-01-01 00:00:00

abstract：:The colicinogenic B factor, transferred from Escherichia coli strain K77 (and termed ColB2-K77 or ColB2) to an E. coli K12 F(-) strain, is capable of promoting its own transfer to other K12 F(-) strains at a low rate (from LFC cultures) which can be increased under special conditions (HFC cultures). LFC cultures of K1...

journal_title：Journal of bacteriology

authors： Hausmann C,Clowes RC

更新日期：1971-09-01 00:00:00

abstract：:Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Myobacterium tuberculosis. J. Bacteriol. 91:2146-2154. 1966.-The ribosomal fraction of the attenuated strain, H37R...

journal_title：Journal of bacteriology

authors： Youmans AS,Youmans GP

更新日期：1966-06-01 00:00:00

abstract：:We analyzed 12 individual codons, which differed widely with respect to the frequency of use in Escherichia coli and the abundance of the corresponding tRNAs, for their influence on the coupling between transcription and translation. This was probed by determining the effects of codon substitutions in the leader pepti...

journal_title：Journal of bacteriology

authors： Bonekamp F,Dalbøge H,Christensen T,Jensen KF

更新日期：1989-11-01 00:00:00

abstract：:The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chlorampheni...

journal_title：Journal of bacteriology

authors： Rodriguez RL,Davern CI

更新日期：1976-01-01 00:00:00

abstract：:The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for ex...

journal_title：Journal of bacteriology

authors： van Keulen G,Dijkhuizen L,Meijer WG

更新日期：2000-08-01 00:00:00

abstract：:A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...

journal_title：Journal of bacteriology

authors： Von Tersch MA,Carlton BC

更新日期：1984-12-01 00:00:00

abstract：:In Escherichia coli K-12 mutants which had a new nalidixic acid resistance mutation at about 82 min on the chromosome map, cell growth was resistant to or hypersusceptible to nalidixic acid, oxolinic acid, piromidic acid, pipemidic acid, and novobiocin. Deoxyribonucleic acid gyrase activity as tested by supercoiling o...

journal_title：Journal of bacteriology

authors： Yamagishi J,Furutani Y,Inoue S,Ohue T,Nakamura S,Shimizu M

更新日期：1981-11-01 00:00:00

abstract：:A delta tyrT::kan mutant from Escherichia coli K-12 (DTK-12) shows a transient growth lag that is caused by glycine starvation (U. Michelsen, M. Bösl, T. Dingermann, and H. Kersten, J. Bacteriol. 171:5987-5994, 1989). The same deletion, transduced into the relA1 spoT1 mutant CA274 to construct strain DTC274, caused co...

journal_title：Journal of bacteriology

authors： Bösl M,Kersten H

更新日期：1994-01-01 00:00:00

abstract：:A Rhodobacter sphaeroides mutant (CYCA1) lacking cytochrome c2 (cyt c2) was previously constructed (T. J. Donohue, A. G. McEwan, S. Van Doren, A. R. Crofts, and S. Kaplan, Biochemistry, 27: 1918-1924, 1988) by a combination of in vivo and in vitro molecular genetic techniques. CYCA1 was incapable of photosynthetic gro...

journal_title：Journal of bacteriology

authors： Brandner JP,McEwan AG,Kaplan S,Donohue TJ

更新日期：1989-01-01 00:00:00

abstract：:The incA gene product of ColE2-P9 and ColE3-CA38 plasmids is an antisense RNA that regulates the production of the plasmid-coded Rep protein essential for replication. The Rep protein specifically binds to the origin and synthesizes a unique primer RNA at the origin. The IncB incompatibility is due to competition for ...

journal_title：Journal of bacteriology

authors： Hiraga S,Sugiyama T,Itoh T

更新日期：1994-12-01 00:00:00

abstract：:Acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. The amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 M propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate in...

journal_title：Journal of bacteriology

authors： Rottem S,Markowitz O

更新日期：1979-12-01 00:00:00

abstract：:Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fastidiosa stress responses. One of the better characterized stress responses in bacteria is the heat shock response, which ind...

journal_title：Journal of bacteriology

authors： Koide T,Vêncio RZ,Gomes SL

更新日期：2006-08-01 00:00:00

abstract：:We examined 14 multiresistant and 8 ampicillin- or tetracycline-resistant Haemophilus influenzae isolates and 4 ampicillin-resistant H. parainfluenzae isolates for plasmid deoxyribonucleic acid. Sixteen strains carried plasmids. Both "plasmid-free" and plasmid-carrying isolates transferred the antibiotic resistance by...

journal_title：Journal of bacteriology

authors： Roberts MC,Smith AL

更新日期：1980-10-01 00:00:00