Abstract:
:Organic compounds exhibit various levels of toxicity toward living organisms based upon their ability to insert into biological membranes and disrupt normal membrane function. The primary mechanism responsible for organic solvent tolerance in many bacteria is energy-dependent extrusion via efflux pumps. One such bacterial strain, Pseudomonas putida S12, is known for its high tolerance to organic solvents as provided through the SrpABC resistance-nodulation-cell division (RND) family efflux pump. To determine how two putative regulatory proteins (SrpR and SrpS, encoded directly upstream of the SrpABC structural genes) influence SrpABC efflux pump expression, we conducted transcriptional analysis, β-galactosidase fusion experiments, electrophoretic mobility shift assays, and pulldown analysis. Together, the results of these experiments suggest that expression of the srpABC operon can be derepressed by two distinct but complementary mechanisms: direct inhibition of the SrpS repressor by organic solvents and binding of SrpS by its antirepressor SrpR.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Sun X,Zahir Z,Lynch KH,Dennis JJdoi
10.1128/JB.00149-11subject
Has Abstractpub_date
2011-06-01 00:00:00pages
2717-25issue
11eissn
0021-9193issn
1098-5530pii
JB.00149-11journal_volume
193pub_type
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