Control of the Staphylococcus aureus toxic shock tst promoter by the global regulator SarA.

Abstract:

:The Staphylococcus aureus SarA global regulator controls the expression of numerous virulence genes, often in conjunction with the agr quorum-sensing system and its effector RNA, RNAIII. In the present study, we have examined the role of both SarA and RNAIII on the regulation of the promoter of tst, encoding staphylococcal superantigen toxic shock syndrome toxin 1 (TSST-1). In vitro DNA-protein interaction studies with purified SarA using gel shift and DNase I protection assays revealed one strong SarA binding site and evidence for a weaker site nearby within the minimal 400-bp promoter region upstream of tst. In vivo analysis of tst promoter activation using a p(tst)-luxAB reporter inserted in the chromosome revealed partial but not complete loss of tst expression in a Δhld-RNAIII strain. In contrast, disruption of sarA abrogated tst expression. No significant tst expression was found for the double Δhld-RNAIII-ΔsarA mutant. Introduction of a plasmid containing cloned hld-RNAIII driven by a non-agr-dependent promoter, p(HU), into isogenic parental wild-type or ΔsarA strains showed comparable levels of RNAIII detected by quantitative reverse transcription-PCR (qRT-PCR) but a two-log(10) reduction in p(tst)-luxAB reporter expression in the ΔsarA strain, arguing that RNAIII levels alone are not strictly determinant for tst expression. Collectively, our results indicate that SarA binds directly to the tst promoter and that SarA plays a significant and direct role in the expression of tst.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Andrey DO,Renzoni A,Monod A,Lew DP,Cheung AL,Kelley WL

doi

10.1128/JB.00146-10

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

6077-85

issue

22

eissn

0021-9193

issn

1098-5530

pii

JB.00146-10

journal_volume

192

pub_type

杂志文章
  • Amplification of DNA at a prophage attachment site in Haemophilus influenzae.

    abstract::The Escherichia coli plasmids pBR322 and pBR327 can be taken up by Haemophilus influenzae but do not replicate in this organism; however, integration of pBR into the H. influenzae chromosome was achieved by ligation to a fragment of the Haemophilus phage S2 that carried a phage attachment site (attP). Once these seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.4.1898-1903.1989

    authors: Kauc L,Goodgal SH

    更新日期:1989-04-01 00:00:00

  • Genetic analysis of pathway specificity during posttranslational protein translocation across the Escherichia coli plasma membrane.

    abstract::In Escherichia coli, the SecB/SecA branch of the Sec pathway and the twin-arginine translocation (Tat) pathway represent two alternative possibilities for posttranslational translocation of proteins across the cytoplasmic membrane. Maintenance of pathway specificity was analyzed using a model precursor consisting of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.9.2811-2819.2003

    authors: Blaudeck N,Kreutzenbeck P,Freudl R,Sprenger GA

    更新日期:2003-05-01 00:00:00

  • Caulobacter crescentus synthesizes an S-layer-editing metalloprotease possessing a domain sharing sequence similarity with its paracrystalline S-layer protein.

    abstract::Strains of Caulobacter crescentus elaborate an S-layer, a two-dimensional protein latticework which covers the cell surface. The S-layer protein (RsaA) is secreted by a type I mechanism (relying on a C-terminal signal) and is unusual among type I secreted proteins because high levels of protein are produced continuous...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.10.2709-2718.2002

    authors: Umelo-Njaka E,Bingle WH,Borchani F,Le KD,Awram P,Blake T,Nomellini JF,Smit J

    更新日期:2002-05-01 00:00:00

  • The herpes simplex virus thymidine kinase gene is not transcribed in Saccharomyces cerevisiae.

    abstract::The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kina...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.2.542-547.1982

    authors: Kiss GB,Pearlman RE,Cornish KV,Friesen JD,Chan VL

    更新日期:1982-02-01 00:00:00

  • Bacteriophage Mu-induced modification of DNA is dependent upon a host function.

    abstract::The DNA of bacteriophage Mu, extracted from induced lysates, is partially resistant to digestion by the endonuclease BalI. This modification of DNA is controlled by the Mu modification function (mom), which acts in conjunction with the dam (DNA-adenine methylation) function of Escherichia coli. Since the BalI recognit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.1.423-428.1978

    authors: Khatoon H,Bukhari AI

    更新日期:1978-10-01 00:00:00

  • Cloning, sequence analysis, and characterization of the genes involved in isoprimeverose metabolism in Lactobacillus pentosus.

    abstract::Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding sit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.9.2312-2320.1998

    authors: Chaillou S,Lokman BC,Leer RJ,Posthuma C,Postma PW,Pouwels PH

    更新日期:1998-05-01 00:00:00

  • FlhD/FlhC is a regulator of anaerobic respiration and the Entner-Doudoroff pathway through induction of the methyl-accepting chemotaxis protein Aer.

    abstract::The regulation by two transcriptional activators of flagellar expression (FlhD and FlhC) and the chemotaxis methyl-accepting protein Aer was studied with glass slide DNA microarrays. An flhD::Kan insertion and an aer deletion were independently introduced into two Escherichia coli K-12 strains, and the effects upon ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.2.534-543.2003

    authors: Prüss BM,Campbell JW,Van Dyk TK,Zhu C,Kogan Y,Matsumura P

    更新日期:2003-01-01 00:00:00

  • Occurrence of pili on and adhesive properties of Haemophilus parainfluenzae.

    abstract::Of 20 clinical isolates of Haemophilus parainfluenzae, 13 produced a mannose-resistant hemagglutinin that agglutinated erythrocytes from chickens, horses, rabbits, and sheep. Examination with the electron microscope showed that only strain HR-885 was pilate. Grown in static liquid culture, the 12 hemagglutinating, non...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.2.1075-1078.1981

    authors: Kahn ME,Gromkova R

    更新日期:1981-02-01 00:00:00

  • Phosphorylation of the group A Streptococcal CovR response regulator causes dimerization and promoter-specific recruitment by RNA polymerase.

    abstract::The group A streptococcus (GAS), Streptococcus pyogenes, is an important human pathogen that causes infections ranging in severity from self-limiting pharyngitis to severe invasive diseases that are associated with significant morbidity and mortality. The pathogenic effects of GAS are mediated by the expression of vir...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00198-06

    authors: Gusa AA,Gao J,Stringer V,Churchward G,Scott JR

    更新日期:2006-07-01 00:00:00

  • Complementation analysis of the cold-sensitive phenotype of the Escherichia coli csdA deletion strain.

    abstract::The cold shock response of Escherichia coli is elicited by downshift of temperature from 37 degrees C to 15 degrees C and is characterized by induction of several cold shock proteins, including CsdA, during the acclimation phase. CsdA, a DEAD-box protein, has been proposed to participate in a variety of processes, suc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00655-07

    authors: Awano N,Xu C,Ke H,Inoue K,Inouye M,Phadtare S

    更新日期:2007-08-01 00:00:00

  • Isolation and characterization of protoplasts from Saccharomyces rouxii.

    abstract::Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.3.1386-1394.1979

    authors: Arnold WN,Garrison RG

    更新日期:1979-03-01 00:00:00

  • Molecular cloning and characterization of two genes for the biotin carboxylase and carboxyltransferase subunits of acetyl coenzyme A carboxylase in Myxococcus xanthus.

    abstract::We have cloned a DNA fragment from a genomic library of Myxococcus xanthus using an oligonucleotide probe representing conserved regions of biotin carboxylase subunits of acetyl coenzyme A (acetyl-CoA) carboxylases. The fragment contained two open reading frames (ORF1 and ORF2), designated the accB and accA genes, cap...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.19.5462-5469.2000

    authors: Kimura Y,Miyake R,Tokumasu Y,Sato M

    更新日期:2000-10-01 00:00:00

  • Effects of streptomycin deprivation on enzyme synthesis in streptomycin-dependent Escherichia coli.

    abstract::During growth of streptomycin-dependent strains of Escherichia coli in the absence of streptomycin (deprived growth), both constitutive and inducible synthesis of beta-galactosidase were preferentially inhibited. A similar preferential inhibition of constitutive and derepressed synthesis of alkaline phosphatase was ob...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.4.1154-1161.1967

    authors: Goodman RE,Spotts CR

    更新日期:1967-10-01 00:00:00

  • Control of arginine utilization in Neurospora.

    abstract::The response of Neurospora to changes in the availibility of exogenous arginine was investigated. Upon addition of arginine to the growth medium, catabolism is initiated within minutes. This occurs prior to expansion of the arginine pool or augmentation of catabolic enzyme levels. (Basal levels are approximately 25% o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.2.866-873.1977

    authors: Weiss RL,Davis RH

    更新日期:1977-02-01 00:00:00

  • DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1.

    abstract::Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. IV. Active transducing phage P1 CM produced by the combination of R factor with phage P1. J. Bacteriol. 88:1266-1276. 1964.-During an investigation of the transduction of R factors with phage P1, a phage lysate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.5.1266-1276.1964

    authors: KONDO E,MITSUHASHI S

    更新日期:1964-11-01 00:00:00

  • Crystal structure of the hyperthermophilic archaeal DNA-binding protein Sso10b2 at a resolution of 1.85 Angstroms.

    abstract::The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.14.4066-4073.2003

    authors: Chou CC,Lin TW,Chen CY,Wang AH

    更新日期:2003-07-01 00:00:00

  • Cyclic AMP in mycobacteria: characterization and functional role of the Rv1647 ortholog in Mycobacterium smegmatis.

    abstract::Mycobacterial genomes are endowed with many eukaryote-like nucleotide cyclase genes encoding proteins that can synthesize 3',5'-cyclic AMP (cAMP). However, the roles of cAMP and the need for such redundancy in terms of adenylyl cyclase genes remain unknown. We measured cAMP levels in Mycobacterium smegmatis during gro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00138-08

    authors: Dass BK,Sharma R,Shenoy AR,Mattoo R,Visweswariah SS

    更新日期:2008-06-01 00:00:00

  • Electromechanical interactions in cell walls of gram-positive cocci.

    abstract::Isolated cell walls of Staphylococcus aureus and Micrococcus lysodeikticus were found to expand and contract in response to changes in environmental pH and ionic strength. These volume changes, which could amount to as much as a doubling of wall dextran-impermeable volume, were related to changes in electrostatic inte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.1.92-101.1970

    authors: Ou LT,Marquis RE

    更新日期:1970-01-01 00:00:00

  • Photosynthetic sulfide oxidation by Chloroflexus aurantiacus, a filamentous, photosynthetic, gliding bacterium.

    abstract::Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.2.782-784.1975

    authors: Madigan MT,Brock TD

    更新日期:1975-05-01 00:00:00

  • Identification of a cytosolically directed NADH dehydrogenase in mitochondria of Saccharomyces cerevisiae.

    abstract::The reoxidation of NADH generated in reactions within the mitochondrial matrix of Saccharomyces cerevisiae is catalyzed by an NADH dehydrogenase designated Ndi1p (C. A. M. Marres, S. de Vries, and L. A. Grivell, Eur. J. Biochem. 195:857-862, 1991). Gene disruption analysis was used to examine possible metabolic functi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.16.4051-4055.1998

    authors: Small WC,McAlister-Henn L

    更新日期:1998-08-01 00:00:00

  • Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands.

    abstract::Strains of Staphylococcus aureus, an important human pathogen, display up to 20% variability in their genome sequence, and most sequence information is available for human clinical isolates that have not been subjected to genetic analysis of virulence attributes. S. aureus strain Newman, which was also isolated from a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01000-07

    authors: Baba T,Bae T,Schneewind O,Takeuchi F,Hiramatsu K

    更新日期:2008-01-01 00:00:00

  • Evidence for a type III secretion system in Aeromonas salmonicida subsp. salmonicida.

    abstract::Aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis, is an important fish pathogen. We have screened this bacterium with a broad-host-range probe directed against yscV, the gene that encodes the archetype of a highly conserved family of inner membrane proteins found in every known type III ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.21.5966-5970.2002

    authors: Burr SE,Stuber K,Wahli T,Frey J

    更新日期:2002-11-01 00:00:00

  • Aerobic metabolism of Streptococcus agalactiae.

    abstract::Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.184-191.1967

    authors: Mickelson MN

    更新日期:1967-07-01 00:00:00

  • Clustering of mutations affecting central pathway enzymes of carbohydrate catabolism in Pseudomonas aeruginosa.

    abstract::Mutations in carbohydrate-negative mutants of Pseudomonas aeruginosa PAO1 individually deficient in glucose 6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), or pyruvate carboxylase (pyc) were mapped on the chromosome by plasmid R68.45-mediated conjugation and by bacteriophage F116L-mediated trans...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.3.1123-1129.1983

    authors: Roehl RA,Feary TW,Phibbs PV Jr

    更新日期:1983-12-01 00:00:00

  • Complete genome sequence of Rickettsia heilongjiangensis, an emerging tick-transmitted human pathogen.

    abstract::Rickettsia heilongjiangensis is an emerging tick-transmitted human pathogen causing far-Eastern spotted fever. Here we report the complete sequence and the main features of the genome of R. heilongjiangensis (strain 054). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05852-11

    authors: Duan C,Tong Y,Huang Y,Wang X,Xiong X,Wen B

    更新日期:2011-10-01 00:00:00

  • Biochemical changes in lysogenic Bacillus stearothermophilus after bacteriophage induction.

    abstract::Welker, N. E. (University of Illinois, Urbana), and L. Leon Campbell. Biochemical changes in lysogenic Bacillus stearothermophilus after bacteriophage induction. J. Bacteriol. 90:1129-1137. 1965.-Cultures of Bacillus stearothermophilus 1503-4R (TP-1) continued to grow at an unaltered rate after induction with mitomyci...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.4.1129-1137.1965

    authors: Welker NE,Campbell LL

    更新日期:1965-10-01 00:00:00

  • Coregulation by phenylacetyl-coenzyme A-responsive PaaX integrates control of the upper and lower pathways for catabolism of styrene by Pseudomonas sp. strain Y2.

    abstract::The P(styA) promoter of Pseudomonas sp. strain Y2 controls expression of the styABCD genes, which are required for the conversion of styrene to phenylacetate, which is further catabolized by the products of two paa gene clusters. Two PaaX repressor proteins (PaaX1 and PaaX2) regulate transcription of the paa gene clus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00176-06

    authors: del Peso-Santos T,Bartolomé-Martín D,Fernández C,Alonso S,García JL,Díaz E,Shingler V,Perera J

    更新日期:2006-07-01 00:00:00

  • Highly conjugative pMG1-like plasmids carrying Tn1546-like transposons that encode vancomycin resistance in Enterococcus faecium.

    abstract::A total of 12 VanA-type vancomycin-resistant enterococci, consisting of 10 Enterococcus faecium isolates and two Enterococcus avium isolates, were examined in detail. The vancomycin resistance conjugative plasmids pHTalpha (65.9 kbp), pHTbeta (63.7 kbp), and pHTgamma (66.5 kbp) were isolated from each of three differe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.23.7024-7028.2003

    authors: Tomita H,Tanimoto K,Hayakawa S,Morinaga K,Ezaki K,Oshima H,Ike Y

    更新日期:2003-12-01 00:00:00

  • Anaerobic regulation of Bacillus subtilis Krebs cycle genes.

    abstract::Krebs cycle enzyme activity in Bacillus subtilis was examined under aerobic and anaerobic conditions. Citrate synthase and aconitase activities in cells grown anaerobically in the presence of nitrate were reduced by as much as 10- and 30-fold, respectively, from levels observed under aerobic culture conditions. The ma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.13.3304-3311.1998

    authors: Nakano MM,Zuber P,Sonenshein AL

    更新日期:1998-07-01 00:00:00

  • Characterization of Vibrio fischeri rRNA operons and subcloning of a ribosomal DNA promoter.

    abstract::Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.2318-2321.1987

    authors: Amikam D,Kuhn J

    更新日期:1987-05-01 00:00:00