Abstract:
:The Staphylococcus aureus SarA global regulator controls the expression of numerous virulence genes, often in conjunction with the agr quorum-sensing system and its effector RNA, RNAIII. In the present study, we have examined the role of both SarA and RNAIII on the regulation of the promoter of tst, encoding staphylococcal superantigen toxic shock syndrome toxin 1 (TSST-1). In vitro DNA-protein interaction studies with purified SarA using gel shift and DNase I protection assays revealed one strong SarA binding site and evidence for a weaker site nearby within the minimal 400-bp promoter region upstream of tst. In vivo analysis of tst promoter activation using a p(tst)-luxAB reporter inserted in the chromosome revealed partial but not complete loss of tst expression in a Δhld-RNAIII strain. In contrast, disruption of sarA abrogated tst expression. No significant tst expression was found for the double Δhld-RNAIII-ΔsarA mutant. Introduction of a plasmid containing cloned hld-RNAIII driven by a non-agr-dependent promoter, p(HU), into isogenic parental wild-type or ΔsarA strains showed comparable levels of RNAIII detected by quantitative reverse transcription-PCR (qRT-PCR) but a two-log(10) reduction in p(tst)-luxAB reporter expression in the ΔsarA strain, arguing that RNAIII levels alone are not strictly determinant for tst expression. Collectively, our results indicate that SarA binds directly to the tst promoter and that SarA plays a significant and direct role in the expression of tst.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Andrey DO,Renzoni A,Monod A,Lew DP,Cheung AL,Kelley WLdoi
10.1128/JB.00146-10subject
Has Abstractpub_date
2010-11-01 00:00:00pages
6077-85issue
22eissn
0021-9193issn
1098-5530pii
JB.00146-10journal_volume
192pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/jb.171.4.1898-1903.1989
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doi:10.1128/JB.149.2.542-547.1982
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1981-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.156.3.1123-1129.1983
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journal_title:Journal of bacteriology
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更新日期:2011-10-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-05-01 00:00:00