Electromechanical interactions in cell walls of gram-positive cocci.

Abstract:

:Isolated cell walls of Staphylococcus aureus and Micrococcus lysodeikticus were found to expand and contract in response to changes in environmental pH and ionic strength. These volume changes, which could amount to as much as a doubling of wall dextran-impermeable volume, were related to changes in electrostatic interactions among fixed, ionized groups in wall polymers, including peptidoglycans. S. aureus walls were structurally more compact in the hydrated state and had a higher maximum charge density than M. lysodeikticus walls. However, they were less responsive to changes in electrostatic interactions, apparently because of less mechanical compliance. In media of nearly neutral pH, S. aureus walls had a net positive charge whereas M. lysodeikticus walls had a net negative charge. These charge differences were reflected in Donnan distributions of mobile ions between wall phases and bulk medium phases. Cell walls of unfractionated cocci also could be made to swell and contract, and wall tonus in intact cells appeared to be set partly by electrostatic interactions and partly by mechanical tension in the elastic structures due to cell turgor pressure. The experimental results led to the conclusions that bacterial cell walls have many of the properties of polyelectrolyte gels and that peptidoglycans are flexible polymers. A reasonable mechanical model for peptidoglycan structure might be a sort of three-dimensional rope ladder with relatively rigid, polysaccharide rungs and relatively flexible polypeptide ropes. Thus, the peptidoglycan network surrounding cocci appeared to be predominantly an elastic restraining structure rather than a rigid shell.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ou LT,Marquis RE

doi

10.1128/JB.101.1.92-101.1970

subject

Has Abstract

pub_date

1970-01-01 00:00:00

pages

92-101

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

101

pub_type

杂志文章
  • Nitrate-dependent regulation of acetate biosynthesis and nitrate respiration by Clostridium thermoaceticum.

    abstract::Nitrate has been shown to shunt the electron flow in Clostridium thermoaceticum from CO2 to nitrate, but it did not influence the levels of enzymes involved in the Wood-Ljungdahl pathway (J. M. Fröstl, C. Seifritz, and H. L. Drake, J. Bacteriol. 178:4597-4603, 1996). Here we show that under some growth conditions, nit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.5.1489-1495.1999

    authors: Arendsen AF,Soliman MQ,Ragsdale SW

    更新日期:1999-03-01 00:00:00

  • Transcription of two sigma 70 homologue genes, sigA and sigB, in stationary-phase Mycobacterium tuberculosis.

    abstract::The sigA and sigB genes of Mycobacterium tuberculosis encode two sigma 70-like sigma factors of RNA polymerase. While transcription of the sigA gene is growth rate independent, sigB transcription is increased during entry into stationary phase. The sigA gene transcription is unresponsive to environmental stress but th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.2.469-476.1999

    authors: Hu Y,Coates AR

    更新日期:1999-01-01 00:00:00

  • Purification of Helicobacter pylori NCTC 11637 cytochrome bc1 and respiration with D-proline as a substrate.

    abstract::Helicobacter pylori is a microaerophilic bacterium associated with gastric inflammation and peptic ulcers. Knowledge of how pathogenic organisms produce energy is important from a therapeutic point of view. We found d-amino acid dehydrogenase-mediated electron transport from d-proline or d-alanine to oxygen via the re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01111-09

    authors: Tanigawa M,Shinohara T,Nishimura K,Nagata K,Ishizuka M,Nagata Y

    更新日期:2010-03-01 00:00:00

  • Isolation and characterization of Escherichia coli tolC mutants defective in secreting enzymatically active alpha-hemolysin.

    abstract::This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.23.6908-6916.2001

    authors: Vakharia H,German GJ,Misra R

    更新日期:2001-12-01 00:00:00

  • Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli.

    abstract::The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5585-5594.1993

    authors: Moralejo P,Egan SM,Hidalgo E,Aguilar J

    更新日期:1993-09-01 00:00:00

  • Comprehensive Spatial Analysis of the Borrelia burgdorferi Lipoproteome Reveals a Compartmentalization Bias toward the Bacterial Surface.

    abstract::The Lyme disease spirochete Borrelia burgdorferi is unique among bacteria in its large number of lipoproteins that are encoded by a small, exceptionally fragmented, and predominantly linear genome. Peripherally anchored in either the inner or outer membrane and facing either the periplasm or the external environment, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00658-16

    authors: Dowdell AS,Murphy MD,Azodi C,Swanson SK,Florens L,Chen S,Zückert WR

    更新日期:2017-02-28 00:00:00

  • Escherichia coli-Salmonella typhimurium hybrid nusA genes: identification of a short motif required for action of the lambda N transcription antitermination protein.

    abstract::The Escherichia coli nusA gene, nusAEc, encodes an essential protein that influences transcription elongation. Derivatives of E. coli in which the Salmonella typhimurium nusA gene, nusASt, has replaced nusAEc are viable. Thus, NusASt can substitute for NusAEc in supporting essential bacterial activities. However, hybr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1394-1404.1994

    authors: Craven MG,Granston AE,Schauer AT,Zheng C,Gray TA,Friedman DI

    更新日期:1994-03-01 00:00:00

  • Buoyant density, growth rate, and the cell cycle in Streptococcus faecium.

    abstract::The buoyant density in rapidly growing Streptococcus faecium 9790 cells varies over the cell cycle, in contrast to the density in Escherichia coli. Buoyant density in S. faecium was measured by using Percoll (Pharmacia Fine Chemicals, Piscataway, N.J.) density gradients. We found that the mean and coefficient of varia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.669-673.1989

    authors: Glaser D,Higgins M

    更新日期:1989-02-01 00:00:00

  • Regulatory role of recF in the SOS response of Escherichia coli: impaired induction of SOS genes by UV irradiation and nalidixic acid in a recF mutant.

    abstract::We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.4.1731-1736.1987

    authors: Thoms B,Wackernagel W

    更新日期:1987-04-01 00:00:00

  • Cytosine methylation of the sequence GATC in a mycoplasma.

    abstract::Mycoplasma virus L2 is subject to host-specific restriction and modification in Acholeplasma laidlawii strains JA1 and K2. We have examined the DNAs from both host cells and viruses propagated on these strains with respect to susceptibility to cleavage by restriction endonucleases and for DNA base modifications. We sh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.3.1420-1424.1982

    authors: Dybvig K,Swinton D,Maniloff J,Hattman S

    更新日期:1982-09-01 00:00:00

  • R-factor-mediated resistance to tetracycline in Proteus mirabilis.

    abstract::The expression of R-factor-mediated resistance to tetracycline has been compared in Proteus mirabilis and Escherichia coli. Resistance to a range of concentrations of tetracycline was significantly lower in P. mirabilis than in E. coli in both induced and repressed states. Indirect evidence showed that conditions whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.235-242.1973

    authors: Franklin TJ,Rownd R

    更新日期:1973-07-01 00:00:00

  • Cluster of genes controlling synthesis and activation of 2,3-dihydroxybenzoic acid in production of enterobactin in Escherichia coli.

    abstract::The Escherichia coli gene cluster encoding enzymatic activities responsible for the synthesis and activation of 2,3-dihydroxybenzoic acid in the formation of the catechol siderophore enterobactin was localized to a 4.2-kilobase chromosomal DNA fragment. Analysis of various subclones and transposon insertion mutations ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4163-4170.1987

    authors: Nahlik MS,Fleming TP,McIntosh MA

    更新日期:1987-09-01 00:00:00

  • An extracellular factor regulates expression of sdiA, a transcriptional activator of cell division genes in Escherichia coli.

    abstract::The sdiA gene codes for a protein that regulates expression of the ftsQAZ cluster of essential cell division genes of Escherichia coli. SdiA up-regulates the ftsQ2p promoter that initiates transcription into the ftsQAZ cluster. In this paper, we report that expression of sdiA is itself regulated by a factor that is re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.10.2742-2748.1996

    authors: García-Lara J,Shang LH,Rothfield LI

    更新日期:1996-05-01 00:00:00

  • Genetics of xanthan production in Xanthomonas campestris: the xanA and xanB genes are involved in UDP-glucose and GDP-mannose biosynthesis.

    abstract::The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of 50,873), respectiv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.1.191-199.1992

    authors: Köplin R,Arnold W,Hötte B,Simon R,Wang G,Pühler A

    更新日期:1992-01-01 00:00:00

  • Functional analysis of luxS in the probiotic strain Lactobacillus rhamnosus GG reveals a central metabolic role important for growth and biofilm formation.

    abstract::Quorum sensing is involved in the regulation of multicellular behavior through communication via small molecules. Given the high number and diversity of the gastrointestinal microbiota, it is postulated that members of this community communicate to coordinate a variety of adaptive processes. AI-2 is suggested to be a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01394-06

    authors: Lebeer S,De Keersmaecker SC,Verhoeven TL,Fadda AA,Marchal K,Vanderleyden J

    更新日期:2007-02-01 00:00:00

  • Comparative studies of lipoteichoic acids from several Bacillus strains.

    abstract::Structural studies were carried out on lipoteichoic acids obtained from defatted cells of 10 Bacillus strains by phenol-water partition followed by chromatography on DEAE-Sephacel and Octyl-Sepharose columns. A group of the tested bacteria (group A), Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus, was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.2.508-516.1986

    authors: Iwasaki H,Shimada A,Ito E

    更新日期:1986-08-01 00:00:00

  • Bacteriocin (hemolysin) of Streptococcus zymogenes.

    abstract::The sensitivity of Streptococcus faecalis (ATTC 8043) to S. zymogenes X-14 bacteriocin depends greatly on its physiological age. Sensitivity decreases from the mid-log phase on and is completely lost in the stationary phase. The sensitivity of erythrocytes to the hemolytic capacity of the bacteriocin showed considerab...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.6.1895-1902.1968

    authors: Basinger SF,Jackson RW

    更新日期:1968-12-01 00:00:00

  • DNA-damaging agents induce the RecA-independent homologous recombination functions of integrating conjugative elements of the SXT/R391 family.

    abstract::Integrating conjugative elements (ICEs) of the SXT/R391 family are major contributors to the spread of antibiotic resistance genes. These elements also catalyze their own diversity by promoting inter-ICE recombination through the action of the RecA-independent homologous recombination system that they encode. Here, we...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02090-12

    authors: Garriss G,Poulin-Laprade D,Burrus V

    更新日期:2013-05-01 00:00:00

  • Tn5401, a new class II transposable element from Bacillus thuringiensis.

    abstract::A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.2835-2845.1994

    authors: Baum JA

    更新日期:1994-05-01 00:00:00

  • Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system.

    abstract::Day, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.1.197-203.1966

    authors: Day LE

    更新日期:1966-07-01 00:00:00

  • Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant.

    abstract::Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.22.7857-7862.2005

    authors: Stevens JM,Ulrich RL,Taylor LA,Wood MW,Deshazer D,Stevens MP,Galyov EE

    更新日期:2005-11-01 00:00:00

  • Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis.

    abstract::Previous determination of the nucleotide sequence of the iol region of the Bacillus subtilis genome allowed us to predict the structure of the iol operon for myo-inositol catabolism, consisting of 10 iol genes (iolA to iouJ); iolG corresponds to idh, encoding myo-inositol 2-dehydrogenase (Idh). Primer extension analys...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.14.4591-4598.1997

    authors: Yoshida KI,Aoyama D,Ishio I,Shibayama T,Fujita Y

    更新日期:1997-07-01 00:00:00

  • Suppression of the defects in rdgB mutants of Escherichia coli K-12 by the cloned purA gene.

    abstract::A gene suppressing the defects of Escherichia coli rdgB (recA-dependent growth) and recA(Ts) rdgB mutants was cloned. The cloned gene suppresses the hyper-Rec phenotype and the enhanced level of SOS functions in rdgB mutants at the nonpermissive temperature. We identified the cloned gene as purA. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.3.1360-1362.1991

    authors: Clyman J,Cunningham RP

    更新日期:1991-02-01 00:00:00

  • Amino acid substitution in the lactose carrier protein with the use of amber suppressors.

    abstract::Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.16.5436-5441.1992

    authors: Huang AM,Lee JI,King SC,Wilson TH

    更新日期:1992-08-01 00:00:00

  • Insight into the Dual Functions of Bacterial Enhancer-Binding Protein Rrp2 of Borrelia burgdorferi.

    abstract:UNLABELLED:It is well established that the RpoN-RpoS sigma factor (σ(54)-σ(S)) cascade plays an essential role in differential gene expression during the enzootic cycle of Borrelia burgdorferi, the causative agent of Lyme disease. The RpoN-RpoS pathway is activated by the response regulator/σ(54)-dependent activator (a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01010-15

    authors: Yin Y,Yang Y,Xiang X,Wang Q,Yang ZN,Blevins J,Lou Y,Yang XF

    更新日期:2016-04-28 00:00:00

  • Identification of a new gene, molR, essential for utilization of molybdate by Escherichia coli.

    abstract::A mutation in a new gene, molR, prevented the synthesis in Escherichia coli of molybdoenzymes, including the two formate dehydrogenase isoenzymes, nitrate reductase and trimethylamine-N-oxide reductase. This phenotype was suppressed by supplementing the media with molybdate. Thus, the molR mutant was phenotypically si...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.4.2079-2087.1990

    authors: Lee JH,Wendt JC,Shanmugam KT

    更新日期:1990-04-01 00:00:00

  • A Bacillus subtilis gene encoding a protein similar to nucleotide sugar transferases influences cell shape and viability.

    abstract::Bacillus subtilis gene ypfP, which is located at 196 degrees on the genetic map, shows similarity to both the monogalactosyldiacylglycerol synthase gene of Cucumis sativus, which encodes a galactosyltransferase, and the murG genes of B. subtilis, Escherichia coli, Haemophilus influenzae, and Synechocystis sp. strain P...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.15.4959-4961.1997

    authors: Price KD,Roels S,Losick R

    更新日期:1997-08-01 00:00:00

  • RELATIONSHIP BETWEEN TOXIGENICITY AND SPORULATING POTENCY OF CLOSTRIDIUM NOVYI.

    abstract::Nishida, Shoki (Kanazawa University, Kanazawa, Japan), and Gizo Nakagawara. Relationship between toxigenicity and sporulating potency of Clostridium novyi. J. Bacteriol. 89:993-995. 1965.-The less toxigenic the strains, the stronger was the sporulating potency of Clostridium novyi strains isolated. This was confirmed ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.4.993-995.1965

    authors: NISHIDA S,NAKAGAWARA G

    更新日期:1965-04-01 00:00:00

  • Multiple formate dehydrogenase enzymes in the facultative methylotroph Methylobacterium extorquens AM1 are dispensable for growth on methanol.

    abstract::Formate dehydrogenase has traditionally been assumed to play an essential role in energy generation during growth on C(1) compounds. However, this assumption has not yet been experimentally tested in methylotrophic bacteria. In this study, a whole-genome analysis approach was used to identify three different formate d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.1.22-28.2004

    authors: Chistoserdova L,Laukel M,Portais JC,Vorholt JA,Lidstrom ME

    更新日期:2004-01-01 00:00:00

  • Glucomannan-mediated attachment of Rhizobium leguminosarum to pea root hairs is required for competitive nodule infection.

    abstract::The Rhizobium leguminosarum biovar viciae genome contains several genes predicted to determine surface polysaccharides. Mutants predicted to affect the initial steps of polysaccharide synthesis were identified and characterized. In addition to the known cellulose (cel) and acidic exopolysaccharide (EPS) (pss) genes, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01694-07

    authors: Williams A,Wilkinson A,Krehenbrink M,Russo DM,Zorreguieta A,Downie JA

    更新日期:2008-07-01 00:00:00