Abstract:
:The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to rhamnulose kinase for rhaB, a 400-amino-acid polypeptide corresponding to rhamnose isomerase for rhaA, and a 274-amino-acid polypeptide corresponding to rhamnulose-1-phosphate aldolase for rhaD. Transcriptional fusions of the three putative promoter regions to lacZ showed that only the rhaB leader region acted as a promoter, as indicated by the high beta-galactosidase activity induced by rhamnose, while no significant activity from the rhaA and rhaD constructions was detected. The rhaB transcription start site was mapped to -24 relative to the start of translation. Mutations in the catabolic genes were used to show that L-rhamnose may directly induce rhaBAD transcription.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Moralejo P,Egan SM,Hidalgo E,Aguilar Jdoi
10.1128/jb.175.17.5585-5594.1993subject
Has Abstractpub_date
1993-09-01 00:00:00pages
5585-94issue
17eissn
0021-9193issn
1098-5530journal_volume
175pub_type
杂志文章abstract::MalK is a peripheral cytoplasmic membrane protein that has multiple activities in Escherichia coli. It associates with integral cytoplasmic membrane proteins MalF and MalG to form the maltose transport complex (MalFGK), a member of the ATP-binding cassette (ABC) superfamily of proteins. In addition, MalK participates ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.4.1337-1343.1997
更新日期:1997-02-01 00:00:00
abstract::Entomopathogenic bacteria of the genus Photorhabdus harbor a type III secretion system. This system was probably acquired prior to the separation of the species within this genus. Furthermore, the core components of the secretion machinery are highly conserved but the predicted effectors differ between Photorhabdus lu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.13.4376-4381.2004
更新日期:2004-07-01 00:00:00
abstract::Unlike mutations to streptomycin resistance, which restrict the function of an SU II amber suppressor, mutations to spectinomycin resistance have no restrictive effect. Furthermore, mutations to spectinomycin resistance counteract the restrictive action of streptomycin-resistant mutations when combined in a single str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.940-943.1969
更新日期:1969-02-01 00:00:00
abstract::Escherichia coli sigma54-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator. Sigma70-dependent transcription of pspF is under autogenous control by wild-type PspF but not by a DNA-binding mutant, PspF deltaHTH. Negative autoregulation of PspF...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.16.5232-5237.1997
更新日期:1997-08-01 00:00:00
abstract::In previous work, we cloned a Candida albicans cDNA for the glycolytic enzyme enolase and found a single, abundant enolase transcript on Northern (RNA) blots and a single protein on immunoblots, using antiserum raised against a recombinant enolase fusion protein. Because C. albicans enolase is abundantly produced duri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.7.1772-1779.1995
更新日期:1995-04-01 00:00:00
abstract::Different electron carriers of the non-desulfoviridin-containing, sulfate-reducing bacterium Desulfovibrio desulfuricans (Norway strain) have been studied. Two nonheme iron proteins, ferredoxin and rubredoxin, have been purified. This ferredoxin contains four atoms of non-heme iron and acid-labile sulfur and six resid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.30-38.1977
更新日期:1977-01-01 00:00:00
abstract::In order to examine the potential role of bacterial collagenases in periodontal tissue destruction, we recently isolated a gene, prtC, from Porphyromonas gingivalis ATCC 53977, which expressed collagenase activity (N. Takahashi, T. Kato, and H. K. Kuramitsu, FEMS Microbiol. Lett. 84:135-138, 1991). The nucleotide sequ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.12.3889-3895.1992
更新日期:1992-06-01 00:00:00
abstract::At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.2.658-667.1970
更新日期:1970-11-01 00:00:00
abstract::Escherichia coli W3110 contains four types of IS1 elements in the chromosome. Using an insertion element entrapping system, we collected 116 IS1 plasmid insertion mutants, which resulted from a minimum of 26 independent IS1 insertion events. All of them had insertions of IS1 of the IS1A (IS1E and IS1G) type. Inspectio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.19.5279-5283.1998
更新日期:1998-10-01 00:00:00
abstract::The Bacillus subtilis ResD-ResE two-component regulatory system activates genes involved in nitrate respiration in response to oxygen limitation or nitric oxide (NO). The sensor kinase ResE activates the response regulator ResD through phosphorylation, which then binds to the regulatory region of genes involved in ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.6.1694-1704.2004
更新日期:2004-03-01 00:00:00
abstract::A thermosensitive episome bearing the transposon Tn10, F(Ts)::Tn10 Lac+, has been successfully transferred from Escherichia coli to several wild strains of the enterobacteria Erwinia carotovora subsp. chrysanthemi, which are pathogenic on Saintpaulia ionantha. In one of these strains, all of the characters controlled ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.1.122-131.1982
更新日期:1982-04-01 00:00:00
abstract::Here, we report the draft genome sequence of Antarctic sea ice bacterium Glaciecola punicea ACAM 611(T), the type species of the genus Glaciecola. A blue-light-absorbing proteorhodopsin gene is present in the 3.08-Mb genome. This genome sequence can facilitate the study of the physiological metabolisms and ecological ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00463-12
更新日期:2012-06-01 00:00:00
abstract::Cytochrome bd oxidase operons from more than 50 species of bacteria contain a short gene encoding a small protein that ranges from ∼30 to 50 amino acids and is predicted to localize to the cell membrane. Although cytochrome bd oxidases have been studied for more than 70 years, little is known about the role of this sm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00324-13
更新日期:2013-08-01 00:00:00
abstract::The antibacterial protein Colicin V (ColV) is secreted from gram-negative bacteria by a signal sequence-independent pathway. The proteins that mediate the export of ColV share sequence similarities with components from other signal sequence-independent export systems such as those for alpha-hemolysin (Hly) and Erwinia...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.23.7549-7556.1991
更新日期:1991-12-01 00:00:00
abstract::The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an Mr of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.398-400.1991
更新日期:1991-01-01 00:00:00
abstract::The gene for an extremely thermostable oligo-1,6-glucosidase (dextrin-6-alpha-D-glucanohydrolase; EC 3.2.1.10) of obligately thermophilic Bacillus thermoglucosidasius KP1006 was cloned within a 4.2-kilobase HindIII-PvuII fragment of DNA by using the plasmid pUC19 as a vector and Escherichia coli C600 as a host. The ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.1219-1222.1989
更新日期:1989-02-01 00:00:00
abstract::Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.2.329-334.1970
更新日期:1970-08-01 00:00:00
abstract::We cloned the cryptic phn operon of a K-12 strain, phn(EcoK), and analyzed the nucleotide sequence of the phn region (11,672 bp). An mRNA start site upstream of the phnC gene was identified by S1 nuclease mapping. The pho regulon activator PhoB protects a pho box region near the mRNA start in DNase I footprinting and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.8.2665-2672.1991
更新日期:1991-04-01 00:00:00
abstract::The dermatophyte Trichophyton schoenleinii elaborates an extracellular collagenase which is specific for native collagen and gelatin and is separable from an enzyme which is active against Azocoll. The fungal enzyme differs from the bacterial collagenases in its acid pH and small molecular weight. In addition, the fun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.43-46.1968
更新日期:1968-01-01 00:00:00
abstract::The single polar flagellum of Pseudomonas aeruginosa plays an important role in the pathogenesis of infection by this organism. However, regulation of the assembly of this organelle has not been delineated. In analyzing the sequence available at the Pseudomonas genome database, an open reading frame (ORF), flanked by ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.2.357-364.2000
更新日期:2000-01-01 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa expresses polar type IV pili (TFP), which are responsible for adhesion to various materials and twitching motility on surfaces. Twitching occurs by alternate extension and retraction of TFP, which arise from assembly and disassembly of pilin subunits at the base of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.3.829-839.2005
更新日期:2005-02-01 00:00:00
abstract::Arcobacter butzleri strain ED-1 is an exoelectrogenic epsilonproteobacterium isolated from the anode biofilm of a microbial fuel cell. Arcobacter sp. strain L dominates the liquid phase of the same fuel cell. Here we report the finished and annotated genome sequences of these organisms. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06084-11
更新日期:2011-11-01 00:00:00
abstract::Open reading frame orf192, which is located immediately upstream of the aerobic repressor gene crtJ, was genetically and biochemically demonstrated to code for a second aerobic repressor (AerR) of photosynthesis gene expression in Rhodobacter capsulatus. Promoter-mapping studies indicate that crtJ has its own promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.10.2805-2814.2002
更新日期:2002-05-01 00:00:00
abstract::The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.14.4066-4073.2003
更新日期:2003-07-01 00:00:00
abstract::It was previously shown that the Rhodobacter capsulatus NtrC enhancer-binding protein activates the R. capsulatus housekeeping RNA polymerase but not the Escherichia coli RNA polymerase at the nifA1 promoter. We have tested the hypothesis that this activity is due to the high G+C content of the -10 sequence. A compara...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.3.740-749.2004
更新日期:2004-02-01 00:00:00
abstract::Four genes encoding for polypeptides with apparent molecular weights of 17,000, 26,000 (the fimbrial subunit), 27,000, and 81,000 have been implicated in the biosynthesis of the K88ab fimbria (Mooi et al., J. Bacteriol. 150:512-521, 1982). Escherichia coli mutants with defects in these genes were examined for the pres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.41-49.1983
更新日期:1983-04-01 00:00:00
abstract::The opportunistic bacterial pathogen Vibrio vulnificus causes severe wound infection and fatal septicemia. We used alkaline phosphatase insertion mutagenesis in a clinical isolate of V. vulnificus to find genes necessary for virulence, and we identified fadR, which encodes a regulator of fatty acid metabolism. The fad...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01016-08
更新日期:2008-12-01 00:00:00
abstract::The smallest viable cell of Spiroplasma citri is a two-turn helix (elementary helix). This elementary helix grows into longer parental cells, which then divide by constriction. The helical morphology is conserved during this process. The growth pattern of S. citri membranes has been investigated by different methods o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.23-28.1984
更新日期:1984-04-01 00:00:00
abstract::Alpha granules were isolated from a blue-green alga, Nostoc muscorum, in large quantities and high purity by sucrose density gradient centrifugation. Sodium deoxycholate was used to eliminate membrane contamination. Isolated alpha granules from this species have average dimension of 31 nm in width and 65 nm in length....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.331-338.1971
更新日期:1971-01-01 00:00:00
abstract::A mutant of Escherichia coli K-12 was examined which has growth medium-dependent lysyl-transfer ribonucleic acid (tRNA) ligase activity. In minimal medium or 0.5% yeast extract, the activity of the enzyme in the mutant strain was 5 to 10% of wild type. However, when the mutant was grown in a highly enriched medium, su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.167-177.1973
更新日期:1973-01-01 00:00:00