Cytosine methylation of the sequence GATC in a mycoplasma.

Abstract:

:Mycoplasma virus L2 is subject to host-specific restriction and modification in Acholeplasma laidlawii strains JA1 and K2. We have examined the DNAs from both host cells and viruses propagated on these strains with respect to susceptibility to cleavage by restriction endonucleases and for DNA base modifications. We show that, in strain K2 and L2 virus grown on K2 cells, cytosine in the sequence GATC is methylated to 5-methylcytosine and, although strain K2 and L2 viruses grown on K2 contain N6-methyladenine in their DNA, adenine in the sequence GATC is not methylated. In contrast to K2, strain JA1 and L2 virus grown on JA1 cells contain no detectable methylated bases. It is not known which of the methylated bases in K2 is the basis for the K2 restriction-modification system operative on L2 virus.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Dybvig K,Swinton D,Maniloff J,Hattman S

doi

10.1128/JB.151.3.1420-1424.1982

subject

Has Abstract

pub_date

1982-09-01 00:00:00

pages

1420-4

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

151

pub_type

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