Abstract:
:The human pathogen Eikenella corrodens expresses type IV pili and exhibits a phase variation involving the irreversible transition from piliated to nonpiliated variants. On solid medium, piliated variants form small (S-phase), corroding colonies whereas nonpiliated variants form large (L-phase), noncorroding colonies. We are studying pilus structure and function in the clinical isolate E. corrodens VA1. Earlier work defined the pilA locus which includes pilA1, pilA2, pilB, and hagA. Both pilA1 and pilA2 predict a type IV pilin, whereas pilB predicts a putative pilus assembly protein. The role of hagA has not been clearly established. That work also confirmed that pilA1 encodes the major pilus protein in this strain and showed that the phase variation involves a posttranslational event in pilus formation. In this study, the function of the individual genes comprising the pilA locus was examined using a recently developed protocol for targeted interposon mutagenesis of S-phase variant VA1-S1. Different pilA mutants were compared to S-phase and L-phase variants for several distinct aspects of phase variation and type IV pilus biosynthesis and function. S-phase cells were characterized by surface pili, competence for natural transformation, and twitching motility, whereas L-phase cells lacked these features. Inactivation of pilA1 yielded a mutant that was phenotypically indistinguishable from L-phase variants, showing that native biosynthesis of the type IV pilus in strain VA1 is dependent on expression of pilA1 and proper export and assembly of PilA1. Inactivation of pilA2 yielded a mutant that was phenotypically indistinguishable from S-phase variants, indicating that pilA2 is not essential for biosynthesis of functionally normal pili. A mutant inactivated for pilB was deficient for twitching motility, suggesting a role for PilB in this pilus-related phenomenon. Inactivation of hagA, which may encode a tellurite resistance protein, had no effect on pilus structure or function.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Villar MT,Hirschberg RL,Schaefer MRdoi
10.1128/JB.183.1.55-62.2001keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
55-62issue
1eissn
0021-9193issn
1098-5530journal_volume
183pub_type
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journal_title:Journal of bacteriology
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更新日期:1978-01-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.11.5119-5124.1987
更新日期:1987-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1982-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2001-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1985-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.33-41.1976
更新日期:1976-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.187.5.1695-1701.2005
更新日期:2005-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.173.18.5784-5792.1991
更新日期:1991-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1969-03-01 00:00:00
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更新日期:1983-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2011-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1975-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1968-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.795-801.1964
更新日期:1964-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2016-08-25 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.4.1364-1368.1999
更新日期:1999-02-01 00:00:00