Abstract:
:Shigella spp. are the major cause of bacillary dysentery worldwide. The pathogenic process involves bacterial invasion and lysis of the phagocytic vacuole, followed by replication and movement within the cell cytoplasm and, ultimately, spread directly into adjacent cells. This study demonstrates that S. flexneri cytochrome bd expression is necessary for normal intracellular survival and virulence. Cytochrome bd is one of two terminal oxidases in the bacterial respiratory chain that reduce molecular oxygen to water, utilizing intermediates shuttled through the electron transport chain. S. flexneri mutants that contain a disruption in the cydC locus, which leads to defective cytochrome bd expression, or in the riboflavin (ribE) or ubiquinol-8 (ubiH) biosynthetic pathway, which leads to elevated cytochrome bd expression, were evaluated in intracellular survival and virulence assays. The cydC mutant formed significantly smaller plaques, had significantly decreased intracellular survival, and had a 100-fold increase in lethal dose for mice compared with the wild type. The ribE and ubiH mutants each formed significantly larger plaques and had a 10-fold decrease in lethal dose for mice compared with the wild type. The data indicate that expression of cytochrome bd is required for S. flexneri intracellular survival and virulence.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Way SS,Sallustio S,Magliozzo RS,Goldberg MBdoi
10.1128/JB.181.4.1229-1237.1999subject
Has Abstractpub_date
1999-02-01 00:00:00pages
1229-37issue
4eissn
0021-9193issn
1098-5530journal_volume
181pub_type
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更新日期:1982-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1970-10-01 00:00:00