Genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834.

Abstract:

:The genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834 has been studied by pulsed-field gel electrophoresis (PFGE). Analysis of its intact DNA, as well as the restriction patterns obtained with several endonucleases, allowed the characterization of one circular chromosome of 2.9 Mb and one plasmid of 8.6 kb. The first complete and highly resolved physical map (86 restriction sites) of the chromosome of an acidophilic obligate chemolithoautotrophic bacterium has been constructed by using endonucleases PmeI, SwaI, XbaI, and SpeI. The rRNA and str operons have been located on the chromosomal physical map.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Irazabal N,Marín I,Amils R

doi

10.1128/jb.179.6.1946-1950.1997

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

1946-50

issue

6

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

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