Abstract:
:DNA sequence analysis revealed that the putative yhdJ DNA methyltransferase gene of Escherichia coli is 55% identical to the Nostoc sp. strain PCC7120 gene encoding DNA methyltransferase AvaIII, which methylates adenine in the recognition sequence, ATGCAT. The yhdJ gene was cloned, and the enzyme was overexpressed and purified. Methylation and restriction analysis showed that the DNA methyltransferase methylates the first adenine in the sequence ATGCAT. This DNA methylation was found to be regulated during the cell cycle, and the DNA adenine methyltransferase was designated M.EcoKCcrM (for "cell cycle-regulated methyltransferase"). The CcrM DNA adenine methyltransferase is required for viability in E. coli, as a strain lacking a functional genomic copy of ccrM can be isolated only in the presence of an additional copy of ccrM supplied in trans. The cells of such a knockout strain stopped growing when expression of the inducible plasmid ccrM gene was shut off. Overexpression of M.EcoKCcrM slowed bacterial growth, and the ATGCAT sites became fully methylated throughout the cell cycle; a high proportion of cells with an anomalous size distribution and DNA content was found in this population. Thus, the temporal control of this methyltransferase may contribute to accurate cell cycle control of cell division and cellular morphology. Homologs of M.EcoKCcrM are present in other bacteria belonging to the gamma subdivision of the class Proteobacteria, suggesting that methylation at ATGCAT sites may have similar functions in other members of this group.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kossykh VG,Lloyd RSdoi
10.1128/jb.186.7.2061-2067.2004keywords:
subject
Has Abstractpub_date
2004-04-01 00:00:00pages
2061-7issue
7eissn
0021-9193issn
1098-5530journal_volume
186pub_type
杂志文章,收录出版abstract::Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to dis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1175-1181.1969
更新日期:1969-12-01 00:00:00
abstract:UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00189-15
更新日期:2015-07-01 00:00:00
abstract::The cell walls of a selected isolate of Staphylococcus aureus FDA 209P were observed undergoing progressive disintegration when exposed to lysostaphin (1 unit/ml) in 24% NaCl solution. Electron micrographs of ultrathin sections of test cells after exposure to lysostaphin for 2 min showed only superficial evidence of l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.1.396-401.1969
更新日期:1969-01-01 00:00:00
abstract::Under anaerobic conditions, Klebsiella pneumoniae reduced nitrite (NO2-), yielding nitrous oxide (N2O) and ammonium ions (NH4+) as products. Nitrous oxide formation accounted for about 5% of the total NO2- reduced, and NH4+ production accounted for the remainder. Glucose and pyruvate were the electron donors for NO2- ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.454-458.1983
更新日期:1983-08-01 00:00:00
abstract::The expression of any given bacterial protein is predicted to depend on (i) the transcriptional regulation of the promoter and the translational regulation of its mRNA and (ii) the synthesis and translation of total (bulk) mRNA. This is because total mRNA acts as a competitor to the specific mRNA for the binding of in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.11.3037-3044.2000
更新日期:2000-06-01 00:00:00
abstract::GspB is a serine-rich glycoprotein adhesin of Streptococcus gordonii that is exported to the bacterial surface by the accessory Sec system. This dedicated export pathway is comprised of seven components (SecA2, SecY2, and five accessory Sec proteins [Asp1 to Asp5]). The latter proteins have no known homologs beyond th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00057-11
更新日期:2011-07-01 00:00:00
abstract::Cyclic 2,3-diphosphoglycerate synthetase (cDPGS) catalyzes the synthesis of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond in 2,3-diphosphoglycerate. cDPG is known to be accumulated to high intracellular concentrations (>300 mM) as a putative thermoadapter in some hyperthe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.22.5997-6004.1998
更新日期:1998-11-01 00:00:00
abstract::Expression of the 146-kilodalton (kDa) extracellular glucoamylase by the budding yeast Schwanniomyces castellii is induced by maltose and starch. By use of antiglucoamylase antisera, we found that this expression was regulated at the level of the mRNA, taking place within 30 min after exposure of yeast cells to the re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.5.2360-2366.1990
更新日期:1990-05-01 00:00:00
abstract::Martinez, R. J. (University of California, Los Angeles), and E. Z. Gordee. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in Spirillum serpens and Bacillus subtilis. J. Bacteriol. 91:870-875. 1966-Exponentially growing cultures of Spirillum serpens and Bacillus subtilis regained motil...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.870-875.1966
更新日期:1966-02-01 00:00:00
abstract::In this study, the H303A mutant form of the cbb(3) oxidase (H303A oxidase), which has the H303A mutation in its catalytic subunit (CcoN), was purified from Rhodobacter sphaeroides. The H303A oxidase showed the same catalytic activity as did the wild-type form of the oxidase (WT oxidase). The heme contents of the mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00443-07
更新日期:2007-08-01 00:00:00
abstract::The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.296-299.1984
更新日期:1984-04-01 00:00:00
abstract::Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00959-09
更新日期:2010-02-01 00:00:00
abstract::Streptococcus suis serotype 2 (S. suis 2) is an important swine and human pathogen responsible for septicemia and meningitis. A novel gene, designated atl and encoding a major autolysin of S. suis 2 virulent strain HA9801, was identified and characterized in this study. The Atl protein contains 1,025 amino acids with ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06231-11
更新日期:2012-03-01 00:00:00
abstract::The xylB and xylE genes in the TOL plasmid of Pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pBR322 in Escherichia coli for detailed mapping. The xylB gene was mapped in a 2.9-kilobase region within the BamHI BC fragment of pTN2, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.3.1137-1143.1981
更新日期:1981-03-01 00:00:00
abstract::Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. Spontaneous mutants which are deficient for virulence and extracellular polysaccharide (Eps) production accumulate in large numbers in stationary-phase cultures of this bacterium, a phenomenon which we have called stationary-phase v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.17.4797-4802.2000
更新日期:2000-09-01 00:00:00
abstract::The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.10.2892-2897.1994
更新日期:1994-05-01 00:00:00
abstract::Clinical isolates of the fungal respiratory and systemic pathogen Histoplasma capsulatum have been placed in several different classes by using genomic restriction fragment length polymorphisms (RFLPs), but in general have not been distinguished further. We report here that a polymerase chain reaction (PCR)-based DNA ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7075-7079.1992
更新日期:1992-11-01 00:00:00
abstract::The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.64-70.1969
更新日期:1969-10-01 00:00:00
abstract::We report here the structure of a previously uncharacterized phospholipid in the radiation-resistant bacterium Deinococcus radiodurans. This phospholipid, designated lipid 4, was shown by chemical analysis, HF hydrolysis, and nuclear magnetic resonance spectroscopy to be phosphatidylglyceroylalkylamine. Lipid 4 thus c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.457-462.1991
更新日期:1991-01-01 00:00:00
abstract::Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract. We sequenced the genome of L. plantarum NC8, a naturally plasmid-free strain, which has been used as a model strain in many labor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.3.1109-1114.1984
更新日期:1984-12-01 00:00:00
abstract::A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.7.2247-2258.1997
更新日期:1997-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.1.250-258.1968
更新日期:1968-07-01 00:00:00
abstract::Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the "downhill" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhyd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.1.187-195.1975
更新日期:1975-07-01 00:00:00
abstract::Transposon Tn5-induced C4-dicarboxylate transport mutants of Rhizobium meliloti 2011 which could be complemented by cosmid pRmSC121 were subdivided into two classes. Class I mutants (RMS37 and RMS938) were defective in symbiotic C4-dicarboxylate transport and in nitrogen fixation. They were mutated in the structural g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5551-5560.1989
更新日期:1989-10-01 00:00:00
abstract::Phosphate is an essential nutrient that Staphylococcus aureus and other pathogens must acquire from the host during infection. While inorganic monophosphate (Pi) is the preferred source of this nutrient, bacteria can also obtain it from phosphate-containing organic molecules. The Pi-responsive regulator PhoPR is neces...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00264-20
更新日期:2020-10-22 00:00:00
abstract::An endonuclease was detected in strains of Salmonella typhimurium containing the drug resistance plasmid pKM101. The enzyme was not detectable in strains lacking this plasmid, but it was present in strains containing mutants of pKM101 that were no longer able to enhance host cell mutagenesis. The endonuclease had a mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.2.583-588.1977
更新日期:1977-08-01 00:00:00
abstract::The endophytic diazotroph Azoarcus sp. strain BH72 is capable of infecting rice roots and of expressing the nitrogenase (nif) genes there. In order to study the genetic background for nitrogen fixation in strain BH72, the structural genes of nitrogenase (nifHDK) were cloned and sequenced. The sequence analysis reveale...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.12.3752-3760.2001
更新日期:2001-06-01 00:00:00
abstract::Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.109-115.1983
更新日期:1983-01-01 00:00:00
abstract::Phosphatidyl ethanolamine and lipopolysaccharide were extracted and purified from the cell envelope fractions of Escherichia coli and Salmonella typhimurium. The two components were studied separately and after recombination, by use of electron microscopy and monolayer techniques, and by measuring their ability to par...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.5.1705-1721.1967
更新日期:1967-05-01 00:00:00