A DNA adenine methyltransferase of Escherichia coli that is cell cycle regulated and essential for viability.

Abstract:

:DNA sequence analysis revealed that the putative yhdJ DNA methyltransferase gene of Escherichia coli is 55% identical to the Nostoc sp. strain PCC7120 gene encoding DNA methyltransferase AvaIII, which methylates adenine in the recognition sequence, ATGCAT. The yhdJ gene was cloned, and the enzyme was overexpressed and purified. Methylation and restriction analysis showed that the DNA methyltransferase methylates the first adenine in the sequence ATGCAT. This DNA methylation was found to be regulated during the cell cycle, and the DNA adenine methyltransferase was designated M.EcoKCcrM (for "cell cycle-regulated methyltransferase"). The CcrM DNA adenine methyltransferase is required for viability in E. coli, as a strain lacking a functional genomic copy of ccrM can be isolated only in the presence of an additional copy of ccrM supplied in trans. The cells of such a knockout strain stopped growing when expression of the inducible plasmid ccrM gene was shut off. Overexpression of M.EcoKCcrM slowed bacterial growth, and the ATGCAT sites became fully methylated throughout the cell cycle; a high proportion of cells with an anomalous size distribution and DNA content was found in this population. Thus, the temporal control of this methyltransferase may contribute to accurate cell cycle control of cell division and cellular morphology. Homologs of M.EcoKCcrM are present in other bacteria belonging to the gamma subdivision of the class Proteobacteria, suggesting that methylation at ATGCAT sites may have similar functions in other members of this group.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kossykh VG,Lloyd RS

doi

10.1128/jb.186.7.2061-2067.2004

keywords:

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

2061-7

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

186

pub_type

杂志文章,收录出版
  • Method for fingerprinting yeast cell wall mannans.

    abstract::Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to dis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.3.1175-1181.1969

    authors: Kocourek J,Ballou CE

    更新日期:1969-12-01 00:00:00

  • The All-Alpha Domains of Coupling Proteins from the Agrobacterium tumefaciens VirB/VirD4 and Enterococcus faecalis pCF10-Encoded Type IV Secretion Systems Confer Specificity to Binding of Cognate DNA Substrates.

    abstract:UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00189-15

    authors: Whitaker N,Chen Y,Jakubowski SJ,Sarkar MK,Li F,Christie PJ

    更新日期:2015-07-01 00:00:00

  • Electron microscopy and viability of lysostaphin-induced staphylococcal spheroplasts, protoplast-like bodies, and protoplasts.

    abstract::The cell walls of a selected isolate of Staphylococcus aureus FDA 209P were observed undergoing progressive disintegration when exposed to lysostaphin (1 unit/ml) in 24% NaCl solution. Electron micrographs of ultrathin sections of test cells after exposure to lysostaphin for 2 min showed only superficial evidence of l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.1.396-401.1969

    authors: Schuhardt VT,Huber TW,Pope LM

    更新日期:1969-01-01 00:00:00

  • Production of nitrous oxide from nitrite in Klebsiella pneumoniae: mutants altered in nitrogen metabolism.

    abstract::Under anaerobic conditions, Klebsiella pneumoniae reduced nitrite (NO2-), yielding nitrous oxide (N2O) and ammonium ions (NH4+) as products. Nitrous oxide formation accounted for about 5% of the total NO2- reduced, and NH4+ production accounted for the remainder. Glucose and pyruvate were the electron donors for NO2- ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.2.454-458.1983

    authors: Satoh T,Hom SS,Shanmugam KT

    更新日期:1983-08-01 00:00:00

  • mRNA composition and control of bacterial gene expression.

    abstract::The expression of any given bacterial protein is predicted to depend on (i) the transcriptional regulation of the promoter and the translational regulation of its mRNA and (ii) the synthesis and translation of total (bulk) mRNA. This is because total mRNA acts as a competitor to the specific mRNA for the binding of in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.11.3037-3044.2000

    authors: Liang ST,Xu YC,Dennis P,Bremer H

    更新日期:2000-06-01 00:00:00

  • Asp2 and Asp3 interact directly with GspB, the export substrate of the Streptococcus gordonii accessory Sec System.

    abstract::GspB is a serine-rich glycoprotein adhesin of Streptococcus gordonii that is exported to the bacterial surface by the accessory Sec system. This dedicated export pathway is comprised of seven components (SecA2, SecY2, and five accessory Sec proteins [Asp1 to Asp5]). The latter proteins have no known homologs beyond th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00057-11

    authors: Yen YT,Seepersaud R,Bensing BA,Sullam PM

    更新日期:2011-07-01 00:00:00

  • Cloning, sequencing, and expression of the gene encoding cyclic 2, 3-diphosphoglycerate synthetase, the key enzyme of cyclic 2, 3-diphosphoglycerate metabolism in Methanothermus fervidus.

    abstract::Cyclic 2,3-diphosphoglycerate synthetase (cDPGS) catalyzes the synthesis of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond in 2,3-diphosphoglycerate. cDPG is known to be accumulated to high intracellular concentrations (>300 mM) as a putative thermoadapter in some hyperthe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.22.5997-6004.1998

    authors: Matussek K,Moritz P,Brunner N,Eckerskorn C,Hensel R

    更新日期:1998-11-01 00:00:00

  • Expression and regulation of glucoamylase from the yeast Schwanniomyces castellii.

    abstract::Expression of the 146-kilodalton (kDa) extracellular glucoamylase by the budding yeast Schwanniomyces castellii is induced by maltose and starch. By use of antiglucoamylase antisera, we found that this expression was regulated at the level of the mRNA, taking place within 30 min after exposure of yeast cells to the re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2360-2366.1990

    authors: Dowhanick TM,Russell I,Scherer SW,Stewart GG,Seligy VL

    更新日期:1990-05-01 00:00:00

  • Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in spirillum serpens and bacillus subtilis.

    abstract::Martinez, R. J. (University of California, Los Angeles), and E. Z. Gordee. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in Spirillum serpens and Bacillus subtilis. J. Bacteriol. 91:870-875. 1966-Exponentially growing cultures of Spirillum serpens and Bacillus subtilis regained motil...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.2.870-875.1966

    authors: Martinez RJ,Gordee EZ

    更新日期:1966-02-01 00:00:00

  • Dominant role of the cbb3 oxidase in regulation of photosynthesis gene expression through the PrrBA system in Rhodobacter sphaeroides 2.4.1.

    abstract::In this study, the H303A mutant form of the cbb(3) oxidase (H303A oxidase), which has the H303A mutation in its catalytic subunit (CcoN), was purified from Rhodobacter sphaeroides. The H303A oxidase showed the same catalytic activity as did the wild-type form of the oxidase (WT oxidase). The heme contents of the mutan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00443-07

    authors: Kim YJ,Ko IJ,Lee JM,Kang HY,Kim YM,Kaplan S,Oh JI

    更新日期:2007-08-01 00:00:00

  • Independence of buoyant cell density and growth rate in Escherichia coli.

    abstract::The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.1.296-299.1984

    authors: Kubitschek HE,Baldwin WW,Schroeter SJ,Graetzer R

    更新日期:1984-04-01 00:00:00

  • Glycerol is metabolized in a complex and strain-dependent manner in Enterococcus faecalis.

    abstract::Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00959-09

    authors: Bizzini A,Zhao C,Budin-Verneuil A,Sauvageot N,Giard JC,Auffray Y,Hartke A

    更新日期:2010-02-01 00:00:00

  • Characterization and functional analysis of atl, a novel gene encoding autolysin in Streptococcus suis.

    abstract::Streptococcus suis serotype 2 (S. suis 2) is an important swine and human pathogen responsible for septicemia and meningitis. A novel gene, designated atl and encoding a major autolysin of S. suis 2 virulent strain HA9801, was identified and characterized in this study. The Atl protein contains 1,025 amino acids with ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06231-11

    authors: Ju CX,Gu HW,Lu CP

    更新日期:2012-03-01 00:00:00

  • Molecular cloning of TOL genes xylB and xylE in Escherichia coli.

    abstract::The xylB and xylE genes in the TOL plasmid of Pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pBR322 in Escherichia coli for detailed mapping. The xylB gene was mapped in a 2.9-kilobase region within the BamHI BC fragment of pTN2, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.3.1137-1143.1981

    authors: Inouye S,Nakazawa A,Nakazawa T

    更新日期:1981-03-01 00:00:00

  • Stationary-phase variation due to transposition of novel insertion elements in Xanthomonas oryzae pv. oryzae.

    abstract::Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. Spontaneous mutants which are deficient for virulence and extracellular polysaccharide (Eps) production accumulate in large numbers in stationary-phase cultures of this bacterium, a phenomenon which we have called stationary-phase v...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.17.4797-4802.2000

    authors: Rajeshwari R,Sonti RV

    更新日期:2000-09-01 00:00:00

  • Plasmid and transposon transfer to Thiobacillus ferrooxidans.

    abstract::The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.2892-2897.1994

    authors: Peng JB,Yan WM,Bao XZ

    更新日期:1994-05-01 00:00:00

  • Diversity among clinical isolates of Histoplasma capsulatum detected by polymerase chain reaction with arbitrary primers.

    abstract::Clinical isolates of the fungal respiratory and systemic pathogen Histoplasma capsulatum have been placed in several different classes by using genomic restriction fragment length polymorphisms (RFLPs), but in general have not been distinguished further. We report here that a polymerase chain reaction (PCR)-based DNA ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.22.7075-7079.1992

    authors: Kersulyte D,Woods JP,Keath EJ,Goldman WE,Berg DE

    更新日期:1992-11-01 00:00:00

  • Mechanism of tuberculostasis in mammalian serum. II. Induction of serum tuberculostasis in guinea pigs.

    abstract::The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.64-70.1969

    authors: Kochan I,Golden CA,Bukovic JA

    更新日期:1969-10-01 00:00:00

  • Phosphatidylglyceroylalkylamine, a novel phosphoglycolipid precursor in Deinococcus radiodurans.

    abstract::We report here the structure of a previously uncharacterized phospholipid in the radiation-resistant bacterium Deinococcus radiodurans. This phospholipid, designated lipid 4, was shown by chemical analysis, HF hydrolysis, and nuclear magnetic resonance spectroscopy to be phosphatidylglyceroylalkylamine. Lipid 4 thus c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.457-462.1991

    authors: Huang Y,Anderson R

    更新日期:1991-01-01 00:00:00

  • Genome sequence of the naturally plasmid-free Lactobacillus plantarum strain NC8 (CCUG 61730).

    abstract::Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract. We sequenced the genome of L. plantarum NC8, a naturally plasmid-free strain, which has been used as a model strain in many labor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00141-12

    authors: Axelsson L,Rud I,Naterstad K,Blom H,Renckens B,Boekhorst J,Kleerebezem M,van Hijum S,Siezen RJ

    更新日期:2012-05-01 00:00:00

  • Return of Streptococcus faecalis DNA cloned in Escherichia coli to its original host via transformation of Streptococcus sanguis followed by conjugative mobilization.

    abstract::Cloning vectors were introduced into Streptococcus faecalis by conjugation. A conjugative plasmid (pVA797) and cloning vector pVA838 recombined in Streptococcus sanguis at homologous sequences, forming a cointegrate. The pVA797::pVA838 cointegrate transferred to S. faecalis by conjugation. Recombination between homolo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.3.1109-1114.1984

    authors: Smith MD,Clewell DB

    更新日期:1984-12-01 00:00:00

  • Multiple loci of Pseudomonas syringae pv. syringae are involved in pathogenicity on bean: restoration of one lesion-deficient mutant requires two tRNA genes.

    abstract::A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.7.2247-2258.1997

    authors: Rich JJ,Willis DK

    更新日期:1997-04-01 00:00:00

  • Pneumonia due to Mycoplasma in gnotobiotic mice. IV. Localization and identification of Mycoplasma pulmonis in the bronchi of infected gnotobiotic mice by immunofluorescence and by light microscopy.

    abstract::The model of pneumonia produced by intranasal inoculation of Mycoplasma pulmonis in gnotobiotic mice provided the opportunity to study the localization and identification of the infecting organisms in the tissues by immunofluorescence techniques. Frozen sections of pneumonic mouse lung were fixed in acetone, layered w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.1.250-258.1968

    authors: Organick AB,Lutsky II

    更新日期:1968-07-01 00:00:00

  • Effect of uncouplers on "downhill" beta-galactoside transport in energy-depleted cells of Escherichia coli.

    abstract::Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the "downhill" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhyd...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.187-195.1975

    authors: Cecchini G,Koch AL

    更新日期:1975-07-01 00:00:00

  • Identification and sequence analysis of the Rhizobium meliloti dctA gene encoding the C4-dicarboxylate carrier.

    abstract::Transposon Tn5-induced C4-dicarboxylate transport mutants of Rhizobium meliloti 2011 which could be complemented by cosmid pRmSC121 were subdivided into two classes. Class I mutants (RMS37 and RMS938) were defective in symbiotic C4-dicarboxylate transport and in nitrogen fixation. They were mutated in the structural g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5551-5560.1989

    authors: Engelke T,Jording D,Kapp D,Pühler A

    更新日期:1989-10-01 00:00:00

  • Staphylococcus aureus Preferentially Liberates Inorganic Phosphate from Organophosphates in Environments where This Nutrient Is Limiting.

    abstract::Phosphate is an essential nutrient that Staphylococcus aureus and other pathogens must acquire from the host during infection. While inorganic monophosphate (Pi) is the preferred source of this nutrient, bacteria can also obtain it from phosphate-containing organic molecules. The Pi-responsive regulator PhoPR is neces...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00264-20

    authors: Kelliher JL,Leder Macek AJ,Grudzinski KM,Radin JN,Kehl-Fie TE

    更新日期:2020-10-22 00:00:00

  • Characterization of an endonuclease associated with the drug resistance plasmid pKM101.

    abstract::An endonuclease was detected in strains of Salmonella typhimurium containing the drug resistance plasmid pKM101. The enzyme was not detectable in strains lacking this plasmid, but it was present in strains containing mutants of pKM101 that were no longer able to enhance host cell mutagenesis. The endonuclease had a mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.2.583-588.1977

    authors: Lackey D,Walker GC,Keng T,Linn S

    更新日期:1977-08-01 00:00:00

  • Role of a ferredoxin gene cotranscribed with the nifHDK operon in N(2) fixation and nitrogenase "switch-off" of Azoarcus sp. strain BH72.

    abstract::The endophytic diazotroph Azoarcus sp. strain BH72 is capable of infecting rice roots and of expressing the nitrogenase (nif) genes there. In order to study the genetic background for nitrogen fixation in strain BH72, the structural genes of nitrogenase (nifHDK) were cloned and sequenced. The sequence analysis reveale...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.12.3752-3760.2001

    authors: Egener T,Martin DE,Sarkar A,Reinhold-Hurek B

    更新日期:2001-06-01 00:00:00

  • Iron supply to Escherichia coli by synthetic analogs of enterochelin.

    abstract::Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.1.109-115.1983

    authors: Heidinger S,Braun V,Pecoraro VL,Raymond KN

    更新日期:1983-01-01 00:00:00

  • Reassociation of purified lipopolysaccharide and phospholipid of the bacterial cell envelope: electron microscopic and monolayer studies.

    abstract::Phosphatidyl ethanolamine and lipopolysaccharide were extracted and purified from the cell envelope fractions of Escherichia coli and Salmonella typhimurium. The two components were studied separately and after recombination, by use of electron microscopy and monolayer techniques, and by measuring their ability to par...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.5.1705-1721.1967

    authors: Rothfield L,Horne RW

    更新日期:1967-05-01 00:00:00