Production of nitrous oxide from nitrite in Klebsiella pneumoniae: mutants altered in nitrogen metabolism.

abstract：:Production of deoxyribonucleotides for DNA synthesis is an essential and tightly regulated process. The class Ia ribonucleotide reductase (RNR), the product of the nrdAB genes, is required for aerobic growth of Escherichia coli. In catalyzing the reduction of ribonucleotides, two of the cysteines of RNR become oxidize...

journal_title：Journal of bacteriology

authors： Feeney MA,Ke N,Beckwith J

更新日期：2012-03-01 00:00:00

abstract：:Short-chain acid-soluble polyphosphates were extracted from growing cultures of Saccharomyces cerevisiae, and the changes in the levels of these compounds were determined. The production of acid-soluble polyphosphates correlated with the mitochondrial activities since it occurred in two bursts in respiration-competent...

journal_title：Journal of bacteriology

authors： Solimene R,Guerrini AM,Donini P

更新日期：1980-08-01 00:00:00

abstract：:The interaction of 12 phage strains with bacilli, protoplasts, and L forms of Bacillus subtilis 168 and with eight of its mutants and two of its lysogens is described qualitatively and quantitatively. After removal of the cell wall from B. subtilis 168, 11 of the 12 phage strains can still adsorb to the protoplasts, n...

journal_title：Journal of bacteriology

authors： Jacobson ED,Landman OE

更新日期：1975-10-01 00:00:00

abstract：:Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als(-). Tra...

journal_title：Journal of bacteriology

authors： Poulsen TS,Chang YY,Hove-Jensen B

更新日期：1999-11-01 00:00:00

abstract：:The catB and catC genes encode cis,cis-muconate lactonizing enzyme I (EC 5.5.1.1) and muconolactone isomerase (EC 5.3.3.4), respectively. These enzymes are required for the dissimilation of benzoate to beta-ketoadipate by Pseudomonas putida and are under coordinate transcriptional regulation. By deletion analysis and ...

journal_title：Journal of bacteriology

authors： Aldrich TL,Chakrabarty AM

更新日期：1988-03-01 00:00:00

abstract：:We purified membrane-bound cytochrome c-550 [cytochrome c-550(m)] to an electrophoretically homogeneous state from Nitrobacter winogradskyi. The cytochrome showed peaks at 409 and 525 nm in the oxidized form and peaks at 416, 521, and 550 nm in the reduced form. The molecular weight of the cytochrome was estimated to ...

journal_title：Journal of bacteriology

authors： Nomoto T,Fukumori Y,Yamanaka T

更新日期：1993-07-01 00:00:00

abstract：:A colorimetric assay was developed for studying the kinetics of iron oxidation with whole cells of the chemoautotroph, Ferrobacillus ferrooxidans. The assay was more advantageous than the conventional method of Warburg manometry because of its simplicity, rapidity, and the small amount of cells required. The assay mea...

journal_title：Journal of bacteriology

authors： Schnaitman CA,Korczynski MS,Lundgren DG

更新日期：1969-08-01 00:00:00

abstract：:Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of c...

journal_title：Journal of bacteriology

authors： Sonstein SA,Baldwin JN

更新日期：1972-01-01 00:00:00

abstract：:The presence of the membrane lipid phosphatidylcholine (PC) in the bacterial membrane is critically important for many host-microbe interactions. The phospholipid N-methyltransferase PmtA from the plant pathogen Agrobacterium tumefaciens catalyzes the formation of PC by a three-step methylation of phosphatidylethanola...

journal_title：Journal of bacteriology

authors： Aktas M,Gleichenhagen J,Stoll R,Narberhaus F

更新日期：2011-07-01 00:00:00

abstract：:Heating at 55 C causes the intracellular degradation of ribosomes of Staphylococcus aureus MF-31. The 30S subunit of heated cells appears to be selectively attacked. Analysis of ribonucleic acid (RNA) showed that 16S RNA was destroyed and that the secondary structure of 23S RNA was altered. ...

journal_title：Journal of bacteriology

authors： Rosenthal LJ,Iandolo JJ

更新日期：1970-09-01 00:00:00

abstract：:The cell walls of a selected isolate of Staphylococcus aureus FDA 209P were observed undergoing progressive disintegration when exposed to lysostaphin (1 unit/ml) in 24% NaCl solution. Electron micrographs of ultrathin sections of test cells after exposure to lysostaphin for 2 min showed only superficial evidence of l...

journal_title：Journal of bacteriology

authors： Schuhardt VT,Huber TW,Pope LM

更新日期：1969-01-01 00:00:00

abstract：:Salmonella enterica serovar Typhimurium that lacks the DNA adenine methylase (Dam) ectopically expresses multiple genes that are preferentially expressed during infection, is attenuated for virulence, and confers heightened immunity in vaccinated hosts. The safety of dam mutant Salmonella vaccines was evaluated by scr...

journal_title：Journal of bacteriology

authors： Heithoff DM,Badie G,Julio SM,Enioutina EY,Daynes RA,Sinsheimer RL,Mahan MJ

更新日期：2007-07-01 00:00:00

abstract：:Two Rhizobium phaseoli mutants, isolated previously by Tn5 mutagenesis, elicited infection threads which ceased development prematurely, usually within root hairs. These infection threads were wide, globular, and otherwise altered in morphology, compared with normal infection threads. Anatomy and division of the root ...

journal_title：Journal of bacteriology

authors： Noel KD,Vandenbosch KA,Kulpaca B

更新日期：1986-12-01 00:00:00

abstract：:Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...

journal_title：Journal of bacteriology

authors： Brunskill EW,Bayles KW

更新日期：1996-02-01 00:00:00

abstract：:Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2...

journal_title：Journal of bacteriology

authors： Chang YF,Feingold DS

更新日期：1970-04-01 00:00:00

abstract：:The pathogenic bacterium Bacillus anthracis has become the subject of intense study as a result of its use in a bioterrorism attack in the United States in September and October 2001. Previous studies suggested that B. anthracis Ames Ancestor, the original Ames fully virulent plasmid-containing isolate, was the ideal ...

journal_title：Journal of bacteriology

authors： Ravel J,Jiang L,Stanley ST,Wilson MR,Decker RS,Read TD,Worsham P,Keim PS,Salzberg SL,Fraser-Liggett CM,Rasko DA

更新日期：2009-01-01 00:00:00

abstract：:The temperate phage TPW22, induced from Lactococcus lactis subsp. cremoris W22, and the evolutionarily interesting integrase of this phage were characterized. Phage TPW22 was propagated lytically on L. lactis subsp. cremoris 3107, which could also be lysogenized by site-specific integration. The attachment site (attP)...

journal_title：Journal of bacteriology

authors： Petersen A,Josephsen J,Johnsen MG

更新日期：1999-11-01 00:00:00

abstract：:The expression of the pur operon, which encodes enzymes of the purine biosynthetic pathway in Bacillus subtilis, is subject to control by the purR gene product (PurR) and phosphoribosylpyrophosphate. This control is also exerted on the purA and purR genes. A consensus sequence for the binding of PurR, named the PurBox...

journal_title：Journal of bacteriology

authors： Saxild HH,Brunstedt K,Nielsen KI,Jarmer H,Nygaard P

更新日期：2001-11-01 00:00:00

abstract：:The Escherichia coli htrD gene was originally isolated during a search for new genes required for growth at high temperature. Insertional inactivation of htrD leads to a pleiotropic phenotype characterized by temperature-sensitive growth in rich medium, H2O2 sensitivity, and sensitivity to cysteine. The htrD gene was ...

journal_title：Journal of bacteriology

authors： Delaney JM,Wall D,Georgopoulos C

更新日期：1993-01-01 00:00:00

abstract：:Genetic transformation of auxotrophs of the extreme thermophile Thermus thermophilus HB27 to prototrophy was obtained at high frequencies of 10(-2) to 10(-1) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent wild-type strain. The transformation frequency was proportio...

journal_title：Journal of bacteriology

authors： Koyama Y,Hoshino T,Tomizuka N,Furukawa K

更新日期：1986-04-01 00:00:00

abstract：:Three genes were shown to provide functions specific for ferric enterobactin transport in Escherichia coli: fepA encoded the outer membrane receptor, fepB produced a periplasmic protein, and the fepC product was presumably a component of a cytoplasmic membrane permease system for this siderophore. A 10.6-kilobase-pair...

journal_title：Journal of bacteriology

authors： Ozenberger BA,Nahlik MS,McIntosh MA

更新日期：1987-08-01 00:00:00

abstract：:The structural properties required for the binding of peptide substrates to the Escherichia coli periplasmic protein involved in oligopeptide transport were surveyed by measuring the ability of different peptides to compete for binding in an equilibrium dialysis assay with the tripeptide Ala-Phe-[3H]Gly. The protein s...

journal_title：Journal of bacteriology

authors： Guyer CA,Morgan DG,Staros JV

更新日期：1986-11-01 00:00:00

abstract：:An approach originally designed to identify functional origins of conjugative transfer (oriT or mob) in a bacterial genome (J. A. Herrera-Cervera, J. M. Sanjuán-Pinilla, J. Olivares, and J. Sanjuán, J. Bacteriol. 180:4583-4590, 1998) was modified to improve its reliability and prevent selection of undesired false mob ...

journal_title：Journal of bacteriology

authors： Pérez-Mendoza D,Domínguez-Ferreras A,Muñoz S,Soto MJ,Olivares J,Brom S,Girard L,Herrera-Cervera JA,Sanjuán J

更新日期：2004-09-01 00:00:00

abstract：:To identify the genes in coliphage 186 that are required for lysogeny, we isolated clear-plaque mutants. Complementation studies and DNA sequencing identified two genes, the cI gene for the immunity maintenance repressor and the cII gene, which is required only for the establishment of lysogeny. One mutant carried a c...

journal_title：Journal of bacteriology

authors： Lamont I,Richardson H,Carter DR,Egan JB

更新日期：1993-08-01 00:00:00

abstract：:Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts. Study of the promoter-lacZ gene fusi...

journal_title：Journal of bacteriology

authors： Weickert MJ,Adhya S

更新日期：1993-01-01 00:00:00

abstract：:Hedgecock, L. W. (Veterans Administration Hospital, Kansas City, Mo.) and R. L. Costello. Utilization of nitrate by pathogenic and saprophytic mycobacteria. J. Bacteriol. 84:195-205. 1962-The ability of mycobacteria to utilize nitrate as a sole source of nitrogen was examined. Nitrate-nitrogen was readily utilized by ...

journal_title：Journal of bacteriology

authors： HEDGECOCK LW,COSTELLO RL

更新日期：1962-08-01 00:00:00

abstract：:A systematic analysis of the type III secretion (T3S) genes of Pseudomonas aeruginosa strain PAO1 revealed that they are under quorum-sensing control. This observation was supported by the down-regulation of the T3S regulon in the presence of RhlR-C4HSL and the corresponding advanced secretion of ExoS in a rhlI mutant...

journal_title：Journal of bacteriology

authors： Bleves S,Soscia C,Nogueira-Orlandi P,Lazdunski A,Filloux A

更新日期：2005-06-01 00:00:00

abstract：:Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine, nopaline, leucinopine, and succinamopine, enhanced this induction 2- to 10-fold. The compounds mannopine, acetopine, arginine,...

journal_title：Journal of bacteriology

authors： Veluthambi K,Krishnan M,Gould JH,Smith RH,Gelvin SB

更新日期：1989-07-01 00:00:00

abstract：:The enterohepatic Helicobacter species Helicobacter hepaticus colonizes the murine intestinal and hepatobiliary tract and is associated with chronic intestinal inflammation, gall stone formation, hepatitis, and hepatocellular carcinoma. Thus far, the role of H. hepaticus motility and flagella in intestinal colonizatio...

journal_title：Journal of bacteriology

authors： Sterzenbach T,Bartonickova L,Behrens W,Brenneke B,Schulze J,Kops F,Chin EY,Katzowitsch E,Schauer DB,Fox JG,Suerbaum S,Josenhans C

更新日期：2008-10-01 00:00:00

abstract：:Intracellular accumulation of nitrate, indicative of the operation of an active nitrate transport system, has been measured in intact cells of the cyanobacterium Anacystis nidulans. The ability of the cells to accumulate nitrate was effectively hindered by either ammonium addition or selective inhibition of CO2 fixati...

journal_title：Journal of bacteriology

authors： Lara C,Romero JM,Guerrero MG

更新日期：1987-09-01 00:00:00