D-glucaric acid and galactaric acid catabolism by Agrobacterium tumefaciens.

Abstract:

:Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. The same enzyme preparation further decarboxylates this intermediate to alpha-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine dinucleotide-dependent reaction to alpha-ketoglutaric acid. Since A. tumefaciens converts d-glucuronic acid to d-glucarate, a pathway from d-glucuronate to alpha-ketoglutarate in A. tumefaciens was determined.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Chang YF,Feingold DS

doi

10.1128/JB.102.1.85-96.1970

subject

Has Abstract

pub_date

1970-04-01 00:00:00

pages

85-96

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

102

pub_type

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