Abstract:
:Eighty-seven different enterobacteria and pseudomonas strains were examined for the presence of extrachromosomal deoxyribonucleic acid (DNA). Thirty-four strains contained closed circular DNA by the ethidium bromide CsCl density technique. Extrachromosomal DNA was most frequent in Escherichia and Klebsiella strains. The extrachromosomal DNA was isolated and characterized by analytical ultracentrifugation and electron microscopy. All the extrachromosomal DNA-containing bacteria contained circular DNA molecules of small size (0.5-4 mum). Most of these bacteria also contained larger circles (20-40 mum). The number of different size classes of circular DNA in each strain varied from one to five. The buoyant density of the extrachromosomal DNA ranged from 1.692 to 1.721 g/cm(3). Many bacteria contained extrachromosomal DNA of more than one density.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Christiansen C,Christiansen G,Bak AL,Stenderup Adoi
10.1128/JB.114.1.367-377.1973subject
Has Abstractpub_date
1973-04-01 00:00:00pages
367-77issue
1eissn
0021-9193issn
1098-5530journal_volume
114pub_type
杂志文章abstract::The conjugative plasmid pTR2030 has been used extensively to confer phage resistance in commercial Lactococcus starter cultures. The plasmid harbors a 16-kb region, flanked by insertion sequence (IS) elements, that encodes the restriction/modification system LlaI and carries an abortive infection gene, abiA. The AbiA ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00904-06
更新日期:2007-02-01 00:00:00
abstract::Transcription of the dnaA gene of the cyanobacterium Synechocystis sp. strain PCC 6803 is light dependent and yields a monocistronic mRNA, as determined by Northern analysis. Surprisingly, mutants with inactivated dnaA were viable. In batch cultures under standard conditions, the mutants grew like the wild type and di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.18.4946-4949.1998
更新日期:1998-09-01 00:00:00
abstract::Membrane vesicles were characterized for their ability to specifically bind [14C]glucose 6-phosphate. Membranes prepared from a strain carrying a ColE1 uhp hybrid plasmid showed significantly enhanced glucose 6-phosphate binding. It is hypothesized that glucose 6-phosphate binding to these membranes is due to a uhpR-d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.1.347-349.1980
更新日期:1980-04-01 00:00:00
abstract::Bacteroides thetaiotaomicron, a gram-negative obligate anaerobe, appears to utilize starch by first binding the polymer to its surface and then translocating it into the periplasmic space. Several genes that encode enzymes or outer membrane proteins involved in starch utilization have been identified. These have been ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.24.7180-7186.1996
更新日期:1996-12-01 00:00:00
abstract::Kobayashi, George S. (Tulane University, New Orleans, La.), Lorraine Friedman, and Judith F. Kofroth. Some cytological and pathogenic properties of spheroplasts of Candida albicans. J. Bacteriol. 88:795-801. 1964.-Spheroplasts of Candida albicans were prepared by use of an enzymatic mixture from the digestive tract of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.795-801.1964
更新日期:1964-09-01 00:00:00
abstract::Genome comparisons have demonstrated that dramatic genetic change often underlies the emergence of new bacterial pathogens. Evolutionary analysis of Escherichia coli O157:H7, a pathogen that has emerged as a worldwide public health threat in the past two decades, has posited that this toxin-producing pathogen evolved ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.5.1783-1791.2005
更新日期:2005-03-01 00:00:00
abstract::Four rpoD hybridizing signals have been identified in the chromosome of Brevibacterium lactofermentum. Two rpoD-like genes, sigA and sigB, have been cloned and sequenced, and they encode principal sigma factors of the RNA polymerase. The deduced amino acid sequences of SigA and SigB showed very high similarities to th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.2.550-553.1996
更新日期:1996-01-01 00:00:00
abstract::The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.14.4066-4073.2003
更新日期:2003-07-01 00:00:00
abstract::A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.3.867-875.1968
更新日期:1968-03-01 00:00:00
abstract::We report the first study of tRNA modification in psychrotolerant archaea, specifically in the archaeon Methanococcoides burtonii grown at 4 and 23 degrees C. For comparison, unfractionated tRNA from the archaeal hyperthermophile Stetteria hydrogenophila cultured at 93 degrees C was examined. Analysis of modified nucl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.18.5483-5490.2003
更新日期:2003-09-01 00:00:00
abstract::The effects of the free bile acids (FBAs) cholic acid (CA), deoxycholic acid (DCA), and chenodeoxycholic acid on the bioenergetics and growth of lactobacilli and bifidobacteria were investigated. It was found that these FBAs reduced the internal pH levels of these bacteria with rapid and stepwise kinetics and, at cert...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.5.1979-1986.2006
更新日期:2006-03-01 00:00:00
abstract::In a horse serum-based medium containing a full complement of fatty acids, cells of Spiroplasma citri were seen to preferentially incorporate palmitic acid. In the same medium, which had a steryl ester-to-sterol ratio of 3.64, a steryl ester-to-sterol ratio of 0.23 was seen in the cells, cholesterol being preferential...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.946-954.1976
更新日期:1976-03-01 00:00:00
abstract::Hirota, Yukinori (University of Osaka, Osaka, Japan), Toshio Fujii, and Yukinobu Nishimura. Loss and repair of conjugal fertility and infectivity of the resistance factor and sex factor in Escherichia coli. J. Bacteriol. 91:1298-1304. 1966.-The drug-resistance factor, R, and the sex factor, F, have homologous traits, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1298-1304.1966
更新日期:1966-03-01 00:00:00
abstract::Trent, Dennis W. (University of Oklahoma School of Medicine, Oklahoma City) and L. Vernon Scott. Colorado tick fever virus in cell culture. I. Cell-type susceptibility and interaction with L cells. J. Bacteriol. 88:702-708. 1964.-Colorado tick fever (CTF) virus was serially propagated in monolayer cultures of L and FL...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.702-708.1964
更新日期:1964-09-01 00:00:00
abstract::Harvey, R. J. (University of California, Davis) and E. B. Collins. Citrate transport system of Streptococcus diacetilactis. J. Bacteriol. 83:1005-1009. 1962.-The uptake of citrate by Streptococcus diacetilactis is mediated by a transport system that was distinguished from passive diffusion by inducibility and kinetics...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.5.1005-1009.1962
更新日期:1962-05-01 00:00:00
abstract::PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F' strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.1.119-128.1972
更新日期:1972-07-01 00:00:00
abstract::A total of 303 isolates of Bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. The population structu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2793-2803.1987
更新日期:1987-06-01 00:00:00
abstract::Adenylate cyclases (ACs) catalyze the formation of 3',5'-cyclic AMP (cAMP) from ATP. A novel AC-encoding gene, cyaC, was isolated from Rhizobium etli by phenotypic complementation of an Escherichia coli cya mutant. The functionality of the cyaC gene was corroborated by its ability to restore cAMP accumulation in an E....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.13.3560-3568.2002
更新日期:2002-07-01 00:00:00
abstract::The tyrT (tRNA(1TYr)) locus of Escherichia coli B differs structurally from that of K-12 strains by the absence of 2 of 3.14 terminal repeat sequences. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2469-2470.1996
更新日期:1996-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.5.1729-1737.1991
更新日期:1991-03-01 00:00:00
abstract::The isolation and some properties of two mutants of Streptococcus faecalis ATCC 9790 (S. faecium) which autolyze at a much slower rate than the wild type are described. Compared with the wild type, mutant E71 autolyzed more slowly, contained less active but more latent autolysin in the isolated wall fraction, and poss...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.1.423-431.1972
更新日期:1972-01-01 00:00:00
abstract::Mitochondrial protein synthesis was studied during release from glucose repression in Saccharomyces cerevisiae cells bearing different mitochondrial genomes. The increase in the rate of the synthesis of mitochondrial translation products was analyzed during respiratory induction. Different kinetic patterns were found ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.3.1125-1132.1983
更新日期:1983-03-01 00:00:00
abstract::The Escherichia coli K42 capsular polysaccharide consists of leads to 3)-alpha-D-Galp-(1 leads to 3)-alpha-D-GalUAp-(1 leads to 3)-alpha-L-Fucp-(1 leads to repeating units. The E. coli K42 and Klebsiella K63 antigens are serologically identical. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.390-391.1978
更新日期:1978-01-01 00:00:00
abstract::The nucleoids in Mycoplasma capricolum cells were visualized by phase-combined fluorescence microscopy of DAPI (4', 6-diamidino-2-phenylindole)-stained cells. Most growing cells in a rich medium had one or two nucleoids in a cell, and no anucleate cells were found. The nucleoids were positioned in the center in mononu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.6073-6080.1999
更新日期:1999-10-01 00:00:00
abstract::Previously, we demonstrated that Escherichia coli tolC mutations reduce the high-level resistance to tetracycline afforded by the transposon Tn10-encoded TetA pump from resistance at 200 microg/ml to resistance at 40 microg/ml. In this study, we found that the addition of an sbmA mutation to a tolC::Tn10 mutant exacer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01844-07
更新日期:2008-02-01 00:00:00
abstract::Heterocysts isolated from Anabaena sp. strain 7120 with lysozyme plus sonication were permeabilized with the cationic detergent cetyltrimethylammonium bromide, and they then exhibited comparable acetylene reduction activity in the light and dark with an ATP-regenerating system plus dithionite. The detergent diminished...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.940-942.1983
更新日期:1983-08-01 00:00:00
abstract::In an effort to identify the Agrobacterium tumefaciens phosphate regulatory gene(s), we isolated a clone from an A. tumefaciens cosmid library that restored regulated alkaline phosphatase activity to an Escherichia coli phoB mutant. The gene that complemented phoB was localized by subcloning and deletion analysis, and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.20.6626-6636.1993
更新日期:1993-10-01 00:00:00
abstract::Acid titrations of intact and butanol-treated cells of Streptococcus faecalis revealed that nearly all of the intracellular K(+) ions could diffuse into the suspending medium in association with small anions, including ribonucleic acid breakdown products, when the cell membrane was damaged. In contrast, nearly all of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.491-498.1973
更新日期:1973-05-01 00:00:00
abstract::Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.109-115.1983
更新日期:1983-01-01 00:00:00