Extrachromosomal deoxyribonucleic acid in different enterobacteria.

Abstract:

:Eighty-seven different enterobacteria and pseudomonas strains were examined for the presence of extrachromosomal deoxyribonucleic acid (DNA). Thirty-four strains contained closed circular DNA by the ethidium bromide CsCl density technique. Extrachromosomal DNA was most frequent in Escherichia and Klebsiella strains. The extrachromosomal DNA was isolated and characterized by analytical ultracentrifugation and electron microscopy. All the extrachromosomal DNA-containing bacteria contained circular DNA molecules of small size (0.5-4 mum). Most of these bacteria also contained larger circles (20-40 mum). The number of different size classes of circular DNA in each strain varied from one to five. The buoyant density of the extrachromosomal DNA ranged from 1.692 to 1.721 g/cm(3). Many bacteria contained extrachromosomal DNA of more than one density.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Christiansen C,Christiansen G,Bak AL,Stenderup A

doi

10.1128/JB.114.1.367-377.1973

subject

Has Abstract

pub_date

1973-04-01 00:00:00

pages

367-77

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

114

pub_type

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