Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis.

Abstract:

:Cells of the cyanobacterium Anabaena variabilis starved for phosphate for 3 days took up phosphate at about 100 times the rate of unstarved cells. Kinetic data suggested that a new transport system had been induced by starvation for phosphate. The inducible phosphate transport system was quickly repressed by addition of Pi. Phosphate-starved cells were more sensitive to the toxic effects of arsenate than were unstarved cells, but phosphate could alleviate some of the toxicity. Arsenate was a noncompetitive inhibitor of phosphate transport; however, the apparent Ki values were high, particularly for phosphate-replete cells. Preincubation of phosphate-starved cells with arsenate caused subsequent inhibition of phosphate transport, suggesting that intracellular arsenate inhibited phosphate transport. This effect was not seen in phosphate-replete cells.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Thiel T

doi

10.1128/jb.170.3.1143-1147.1988

subject

Has Abstract

pub_date

1988-03-01 00:00:00

pages

1143-7

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

170

pub_type

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