Isolation and expansion of the catabolic potential of a Pseudomonas putida strain able to grow in the presence of high concentrations of aromatic hydrocarbons.

Abstract:

:Pseudomonas putida DOT-T1 was isolated after enrichment on minimal medium with 1% (vol/vol) toluene as the sole C source. The strain was able to grow in the presence of 90% (vol/vol) toluene and was tolerant to organic solvents whose log P(ow) (octanol/water partition coefficient) was higher than 2.3. Solvent tolerance was inducible, as bacteria grown in the absence of toluene required an adaptation period before growth restarted. Mg2+ ions in the culture medium improved solvent tolerance. Electron micrographs showed that cells growing on high concentrations of toluene exhibited a wider periplasmic space than cells growing in the absence of toluene and preserved the outer membrane integrity. Polarographic studies and the accumulation of pathway intermediates showed that the strain used the toluene-4-monooxygenase pathway to catabolyze toluene. Although the strain also thrived in high concentrations of m- and p-xylene, these hydrocarbons could not be used as the sole C source for growth. The catabolic potential of the isolate was expanded to include m- and p-xylene and related hydrocarbons by transfer of the TOL plasmid pWW0-Km.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ramos JL,Duque E,Huertas MJ,Haïdour A

doi

10.1128/jb.177.14.3911-3916.1995

subject

Has Abstract

pub_date

1995-07-01 00:00:00

pages

3911-6

issue

14

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

杂志文章
  • Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I.

    abstract::A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.12.4166-4168.1992

    authors: Witkin EM,Roegner-Maniscalco V

    更新日期:1992-06-01 00:00:00

  • Levels and activity of the Pseudomonas putida global regulatory protein Crc vary according to growth conditions.

    abstract::The global regulatory protein Crc is involved in the repression of several catabolic pathways for sugars, hydrocarbons, and nitrogenated and aromatic compounds in Pseudomonas putida and Pseudomonas aeruginosa when other preferred carbon sources are present in the culture medium (catabolite repression), therefore modul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.11.3678-3686.2005

    authors: Ruiz-Manzano A,Yuste L,Rojo F

    更新日期:2005-06-01 00:00:00

  • Identification of a cytosolically directed NADH dehydrogenase in mitochondria of Saccharomyces cerevisiae.

    abstract::The reoxidation of NADH generated in reactions within the mitochondrial matrix of Saccharomyces cerevisiae is catalyzed by an NADH dehydrogenase designated Ndi1p (C. A. M. Marres, S. de Vries, and L. A. Grivell, Eur. J. Biochem. 195:857-862, 1991). Gene disruption analysis was used to examine possible metabolic functi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.16.4051-4055.1998

    authors: Small WC,McAlister-Henn L

    更新日期:1998-08-01 00:00:00

  • Methionine sulfoxide reductase from the hyperthermophilic archaeon Thermococcus kodakaraensis, an enzyme designed to function at suboptimal growth temperatures.

    abstract::Methionine sulfoxide reductase (Msr) catalyzes the thioredoxin-dependent reduction and repair of methionine sulfoxide (MetO). Although Msr genes are not present in most hyperthermophile genomes, an Msr homolog encoding an MsrA-MsrB fusion protein (MsrAB(Tk)) was present on the genome of the hyperthermophilic archaeon ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00751-06

    authors: Fukushima E,Shinka Y,Fukui T,Atomi H,Imanaka T

    更新日期:2007-10-01 00:00:00

  • Autoinduction of 2,4-diacetylphloroglucinol biosynthesis in the biocontrol agent Pseudomonas fluorescens CHA0 and repression by the bacterial metabolites salicylate and pyoluteorin.

    abstract::The antimicrobial metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) contributes to the capacity of Pseudomonas fluorescens strain CHA0 to control plant diseases caused by soilborne pathogens. A 2, 4-DAPG-negative Tn5 insertion mutant of strain CHA0 was isolated, and the nucleotide sequence of the 4-kb genomic DNA regio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.5.1215-1225.2000

    authors: Schnider-Keel U,Seematter A,Maurhofer M,Blumer C,Duffy B,Gigot-Bonnefoy C,Reimmann C,Notz R,Défago G,Haas D,Keel C

    更新日期:2000-03-01 00:00:00

  • Multiple promoter inversions generate surface antigenic variation in Mycoplasma penetrans.

    abstract::Mycoplasma penetrans is a newly identified species of the genus MYCOPLASMA: It was first isolated from a urine sample from a human immunodeficiency virus (HIV)-infected patient. M. penetrans changes its surface antigen profile with high frequency. The changes originate from ON<==>OFF phase variations of the P35 family...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.1.231-242.2003

    authors: Horino A,Sasaki Y,Sasaki T,Kenri T

    更新日期:2003-01-01 00:00:00

  • Ultrastructural analysis during germination and outgrowth of Bacillus subtilis spores.

    abstract::Electron microscopy of thin sections of dormant and germinating spores of Bacillus subtilis 168 revealed a progressive change in the structure of the cortex, outer spore coat, and inner spore coat. The initial changes were observed in the cortex region, which showed a loose fibrous network within 10 min of germination...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.1.475-481.1974

    authors: Santo LY,Doi RH

    更新日期:1974-10-01 00:00:00

  • Replicative deoxyribonucleic acid synthesis in isolated mitochondria from Saccharomyces cerevisiae.

    abstract::The characteristics of a system for the in vitro synthesis of mitochondrial deoxyribonucleic acid (mtDNA) in mitochondria isolated from Saccharomyces cerevisiae are described. In this system the exclusive product of the reaction is mtDNA. Under optimal conditions the initial rate of synthesis is close to the calculate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.3.973-982.1977

    authors: Mattick JS,Hall RM

    更新日期:1977-06-01 00:00:00

  • Homology of a plasmid from the spirochete Treponema denticola with the single-stranded DNA plasmids.

    abstract::The 2,647-bp nucleotide sequence of cryptic plasmid pTD1, isolated from the oral spirochete Treponema denticola, was determined. The sequence revealed two open reading frames, A and B, which encode polypeptides of 335 and 235 amino acids, respectively. Open reading frame A shows sequence similarity to genes that encod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.8.2724-2728.1992

    authors: MacDougall J,Margarita D,Saint Girons I

    更新日期:1992-04-01 00:00:00

  • Tumor induction by Agrobacterium tumefaciens: specific transfer of bacterial deoxyribonucleic acid to plant tissue.

    abstract::When Agrobacterium tumefaciens cells grown in the presence of tritiated thymidine to label specifically the bacterial deoxyribonucleic acid (DNA) are incubated with carrot root tissue for short periods of time, an appreciable fraction of the label becomes firmly associated with the root tissue. Such association is not...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.973-979.1971

    authors: Yajko DM,Hegeman GD

    更新日期:1971-12-01 00:00:00

  • Transcriptional regulation of the phosphotransacetylase-encoding and acetate kinase-encoding genes (pta and ack) from Methanosarcina thermophila.

    abstract::Phosphotransacetylase and acetate kinase catalyze the activation of acetate to acetyl coenzyme A in the first step of methanogenesis from acetate in Methanosarcina thermophila. The genes encoding these enzymes (pta and ack) have been cloned and sequenced. They are arranged on the chromosome with pta upstream of ack (M...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.7.1699-1702.1995

    authors: Singh-Wissmann K,Ferry JG

    更新日期:1995-04-01 00:00:00

  • GENETIC INSTABILITY IN ESCHERICHIA COLI.

    abstract::Schwartz, Norman M. (Yale University, New Haven, Conn.). Genetic instability in Escherichia coli. J. Bacteriol. 89:712-717. 1965.-Results obtained from a transductional analysis demonstrated that an extremely unstable reversion of lac(-) is a suppressor mutation. This mutation suppresses 2 of 22 different lac(-) allel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.3.712-717.1965

    authors: SCHWARTZ NM

    更新日期:1965-03-01 00:00:00

  • Taxonomy of psychrophilic strains of Bacillus.

    abstract::The morphological and physiological characteristics of 20 isolates of psychrophilic Bacillus were compared with 29 strains representing nine species of mesophilic Bacillus and 2 strains of Sporosarcina ureae to determine the taxonomic position of the psychrophiles. The psychrophiles formed four distinct groups which w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.4.889-895.1967

    authors: Larkin JM,Stokes JL

    更新日期:1967-10-01 00:00:00

  • Activation of ATP:GTP 3'-pyrophosphotransferase (guanosine pentaphosphate synthetase) in Streptomyces antibioticus.

    abstract::The activity of the ATP:GTP 3'-pyrophosphotransferase (guanosine pentaphosphate synthetase I [GPSI]) from Streptomyces antibioticus is stimulated maximally by methanol at 20% (vol/vol) in assay mixtures. Although the enzyme is not activated by ribosomes, its activity is stimulated by tRNA (uncharged or charged) and by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1482-1487.1994

    authors: Jones GH

    更新日期:1994-03-01 00:00:00

  • Physiological and transcriptional responses to osmotic stress of two Pseudomonas syringae strains that differ in epiphytic fitness and osmotolerance.

    abstract::The foliar pathogen Pseudomonas syringae is a useful model for understanding the role of stress adaptation in leaf colonization. We investigated the mechanistic basis of differences in the osmotolerance of two P. syringae strains, B728a and DC3000. Consistent with its higher survival rates following inoculation onto l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00787-13

    authors: Freeman BC,Chen C,Yu X,Nielsen L,Peterson K,Beattie GA

    更新日期:2013-10-01 00:00:00

  • Protein-tyrosine phosphorylation in the Archaea.

    abstract::Sulfolobus sulfataricus ATCC 35091, Haloferax volcanii, and Methanosarcina thermophila TM-1, representing the Euryarchaeota and Crenarchaeota subdomains of the Archaea, contain proteins which are phosphorylated on tyrosine. These data raise fundamental questions as to the origin and evolution of tyrosine phosphorylati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.7.2418-2420.1997

    authors: Smith SC,Kennelly PJ,Potts M

    更新日期:1997-04-01 00:00:00

  • The binding site of the IclR repressor protein overlaps the promoter of aceBAK.

    abstract::In Escherichia coli, repression of the aceBAK operon is mediated by the IclR protein. We used an in vitro oligonucleotide selection technique to determine the consensus recognition sequence for MR. Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.13.3982-3984.1996

    authors: Pan B,Unnikrishnan I,LaPorte DC

    更新日期:1996-07-01 00:00:00

  • Amino acid residues in LuxR critical for its mechanism of transcriptional activation during quorum sensing in Vibrio fischeri.

    abstract::PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the v...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.1.387-392.2001

    authors: Trott AE,Stevens AM

    更新日期:2001-01-01 00:00:00

  • Growth, reproduction, and death rates of Escherichia coli at increased hydrostatic pressures.

    abstract::ZoBell, Claude E. (University of California, La Jolla) and Andre B. Cobet. Growth, reproduction, and death rates of Escherichia coli at increased hydrostatic pressures. J. Bacteriol. 84:1228-1236. 1962.-Pressures ranging from 100 to 500 atm were found to retard the growth and reproduction of Escherichia coli in nutrie...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.6.1228-1236.1962

    authors: ZOBELL CE,COBET AB

    更新日期:1962-12-01 00:00:00

  • Glucose metabolism in gcr mutants of Saccharomyces cerevisiae.

    abstract::A gcr2 null mutant of Saccharomyces cerevisiae grows well on glucose in spite of its lower level of glycolytic enzymes between triose phosphates and pyruvate. A quantitative analysis shows that these levels are adequate to the flux but glycerate phosphates are elevated. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.15.4719-4723.1999

    authors: Uemura H,Fraenkel DG

    更新日期:1999-08-01 00:00:00

  • Stringent control of peptidoglycan biosynthesis in Escherichia coli K-12.

    abstract::[3H]Diaminopimelic acid (Dap) was incorporated exclusively into peptidoglycan by Escherichia coli strains auxotrophic for both lysine and Dap. The rate of [3H]Dap incorporation by stringent (rel+) strains was significantly decreased when cells were deprived of required amino acids. The addition of chloramphenicol to a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1119-1126.1976

    authors: Ishiguro EE,Ramey WD

    更新日期:1976-09-01 00:00:00

  • Lipidation of an FlrC-dependent protein is required for enhanced intestinal colonization by Vibrio cholerae.

    abstract::Vibrio cholerae, the causative agent of cholera, has a sheathed, polar flagellum, and motility has been linked to virulence. An operon with two genes, flgO and flgP (VC2207 and VC2206), is positively regulated by FlrC, the activator of class III flagellar genes. Deletion of flgP results in a nonmotile phenotype, demon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00924-07

    authors: Morris DC,Peng F,Barker JR,Klose KE

    更新日期:2008-01-01 00:00:00

  • Effect of a 20-kilodalton protein from Bacillus thuringiensis subsp. israelensis on production of the CytA protein by Escherichia coli.

    abstract::CytA, a 27-kDa cytolytic crystal protein of Bacillus thuringiensis subsp. israelensis, is produced only at very low levels by recombinant Escherichia coli cells unless a 20-kDa B. thuringiensis subsp. israelensis protein is also present (K. M. McLean and H. R. Whiteley, J. Bacteriol. 169:1017-1023, 1987; L. F. Adams, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.5.1748-1756.1991

    authors: Visick JE,Whiteley HR

    更新日期:1991-03-01 00:00:00

  • Mutations in residues involved in zinc binding in the catalytic site of Escherichia coli threonyl-tRNA synthetase confer a dominant lethal phenotype.

    abstract::Escherichia coli threonyl-tRNA synthetase is a homodimeric protein that acts as both an enzyme and a regulator of gene expression: the protein aminoacylates tRNA(Thr) isoacceptors and binds to its own mRNA, inhibiting its translation. The enzyme contains a zinc atom in its active site, which is essential for the recog...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00439-07

    authors: Caillet J,Graffe M,Eyermann F,Romby P,Springer M

    更新日期:2007-10-01 00:00:00

  • Characterization of the primary immunity region of the Escherichia coli linear plasmid prophage N15.

    abstract::N15 is the only bacteriophage of Escherichia coli known to lysogenize as a linear plasmid. Clear-plaque mutations lie in at least two regions of the 46-kb genome. We have cloned, sequenced, and characterized the primary immunity region, immB. This region contains a gene, cB, whose product shows homology to lambdoid ph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.10.2902-2910.1996

    authors: Lobocka MB,Svarchevsky AN,Rybchin VN,Yarmolinsky MB

    更新日期:1996-05-01 00:00:00

  • The extent of migration of the Holliday junction is a crucial factor for gene conversion in Rhizobium etli.

    abstract::Gene conversion, defined as the nonreciprocal transfer of DNA, is one result of homologous recombination. Three steps in recombination could give rise to gene conversion: (i) DNA synthesis for repair of the degraded segment, (ii) Holliday junction migration, leading to heteroduplex formation, and (iii) repair of misma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00111-09

    authors: Castellanos M,Romero D

    更新日期:2009-08-01 00:00:00

  • The O-antigen gene cluster of Escherichia coli O55:H7 and identification of a new UDP-GlcNAc C4 epimerase gene.

    abstract::Escherichia coli O55 is an important antigen which is often associated with enteropathogenic E. coli clones. We sequenced the genes responsible for its synthesis and identified genes for O-antigen polymerase, O-antigen flippase, four enzymes involved in GDP-colitose synthesis, and three glycosyltransferases, all by co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.10.2620-2625.2002

    authors: Wang L,Huskic S,Cisterne A,Rothemund D,Reeves PR

    更新日期:2002-05-01 00:00:00

  • Characterization of the detachable Rho-dependent transcription terminator of the fimE gene in Escherichia coli K-12.

    abstract::The fim genetic switch in the chromosome of Escherichia coli K-12 is an invertible DNA element that harbors the promoter for transcription of the downstream fim structural genes and a transcription terminator that acts on the upstream fimE regulatory gene. Switches oriented appropriately for structural gene transcript...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.24.8256-8266.2005

    authors: Hinde P,Deighan P,Dorman CJ

    更新日期:2005-12-01 00:00:00

  • A four-dimensional view of assembly of a morphogenetic protein during sporulation in Bacillus subtilis.

    abstract::We report the use of a fusion to the green fluorescent protein to visualize the assembly of the morphogenetic protein SpoIVA around the developing forespore during the process of sporulation in the bacterium Bacillus subtilis. Using a deconvolution algorithm to process digitally-collected optical sections, we show tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.3.781-790.1999

    authors: Price KD,Losick R

    更新日期:1999-02-01 00:00:00

  • Addition of Escherichia coli K-12 growth observation and gene essentiality data to the EcoCyc database.

    abstract::The sets of compounds that can support growth of an organism are defined by the presence of transporters and metabolic pathways that convert nutrient sources into cellular components and energy for growth. A collection of known nutrient sources can therefore serve both as an impetus for investigating new metabolic pat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01209-13

    authors: Mackie A,Paley S,Keseler IM,Shearer A,Paulsen IT,Karp PD

    更新日期:2014-03-01 00:00:00