The Acinetobacter baylyi Hfq gene encodes a large protein with an unusual C terminus.

abstract：:In streptomycete anthracycline biosynthetic gene clusters, small open reading frames are located just upstream of minimal polyketide synthase genes. aknX is such a gene found in the aklavinone-aclacinomycin biosynthetic gene cluster of Streptomyces galilaeus. In order to identify its function, the aknX gene was expres...

journal_title：Journal of bacteriology

authors： Chung JY,Fujii I,Harada S,Sankawa U,Ebizuka Y

更新日期：2002-11-01 00:00:00

abstract：:The medium in which Rec(+) strains of Escherichia coli K-12 are grown affected their sensitivity to treatment with methyl methane sulfonate (MMS). Rec(+) cells grown to the stationary phase in glucose-enriched nutrient broth (GNB) were more resistant to MMS than cells grown in nutrient broth (NB). The repair of MMS-in...

journal_title：Journal of bacteriology

authors： Scudiero DA,Friesen BS,Baptist JE

更新日期：1973-04-01 00:00:00

abstract：:We have identified recD mutants of Salmonella typhimurium by their ability to support growth of phage P22 abc (anti-RecBCD) mutants, whose growth is prevented by normal host RecBCD function. As in Escherichia coli, the recD gene of S. typhimurium lies between the recB and argA genes at min 61 of the genetic map. Plasm...

journal_title：Journal of bacteriology

authors： Miesel L,Roth JR

更新日期：1994-07-01 00:00:00

abstract：:Acyl-homoserine lactone (acyl-HSL) quorum sensing is common to many Proteobacteria including a clinical isolate of Burkholderia cepacia. The B. cepacia isolate produces low levels of octanoyl-HSL. We have examined an environmental isolate of Burkholderia vietnamiensis. This isolate produced several acyl-HSLs. The most...

journal_title：Journal of bacteriology

authors： Conway BA,Greenberg EP

更新日期：2002-02-01 00:00:00

abstract：:tRNAs play a critical role in mRNA decoding, and posttranscriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and lo...

journal_title：Journal of bacteriology

authors： Yu N,Jora M,Solivio B,Thakur P,Acevedo-Rocha CG,Randau L,de Crécy-Lagard V,Addepalli B,Limbach PA

更新日期：2019-04-09 00:00:00

abstract：:The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

journal_title：Journal of bacteriology

authors： Katagiri M,Wheelis ML

更新日期：1971-05-01 00:00:00

abstract：:Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...

journal_title：Journal of bacteriology

authors： Franzetti B,Sohlberg B,Zaccai G,von Gabain A

更新日期：1997-02-01 00:00:00

abstract：:The virulence factors of the cariogenic bacterium Streptococcus sobrinus have been difficult to assess because of a lack of tools for the genetic manipulation of this organism. The construction of an Escherichia coli-Streptococcus shuttle vector, pDL289, that can be mobilized into S. sobrinus by the conjugative plasmi...

journal_title：Journal of bacteriology

authors： Buckley ND,Lee LN,LeBlanc DJ

更新日期：1995-09-01 00:00:00

abstract：:The nucleotide sequence of a region located downstream of the nifB gene, both in the cyanobacterium Anabaena sp. strain PCC 7120 and in Rhizobium meliloti, has been determined. This region contains a gene (fdxN) whose predicted polypeptide product strongly resembles typical bacterial ferredoxins. Cyanobacteria have no...

journal_title：Journal of bacteriology

authors： Mulligan ME,Buikema WJ,Haselkorn R

更新日期：1988-09-01 00:00:00

abstract：:A method consisting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequent detection of endoglucanases by blotting with a polyclonal antibody against endoglucanase I was used to investigate the effect of induction and carbon catabolite derepression on the synthesis of multiple forms of endoglucana...

journal_title：Journal of bacteriology

authors： Messner R,Gruber F,Kubicek CP

更新日期：1988-08-01 00:00:00

abstract：:Four genes, dra, tpp, drm, and pup, that specify enzymes involved in the catabolism of nucleosides and deoxynucleosides in Escherichia coli are known to be very closely linked in the order dra-tpp-drm-pup. By infecting cells with the phage Mu-1 and isolating low-thymine-requiring derivatives of a strain lacking thymid...

journal_title：Journal of bacteriology

authors： Buxton RS

更新日期：1975-02-01 00:00:00

abstract：:The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...

journal_title：Journal of bacteriology

authors： Safo MK,Musayev FN,Hunt S,di Salvo ML,Scarsdale N,Schirch V

更新日期：2004-12-01 00:00:00

abstract：:Protein synthesis in Allomyces marcogynus mitospores began at the time of encystment and was required for further germination. The rate of protein synthesis in germinating spores decreased in the absence of ribonucleic acid synthesis relative to the uninhibited control. There was a concomitant decrease in polysome con...

journal_title：Journal of bacteriology

authors： Smith BA,Burke DD

更新日期：1980-09-01 00:00:00

abstract：:Farrar, W. Edmund, Jr. (Walter Reed Army Institute of Research, Washington, D.C.), Thomas H. Kent, and Van B. Elliott. Lethal gram-negative bacterial superinfection in guinea pigs given bacitracin. J. Bacteriol. 92:496-501. 1966.-Oral administration of a single dose of bacitracin (either 2,000 or 10,000 units) was let...

journal_title：Journal of bacteriology

authors： Farrar WE,Kent TH,Elliott VB

更新日期：1966-08-01 00:00:00

abstract：:The Tm of Bacillus stearothermophilus 5S ribonucleic acid (RNA) is 1.5 +/- 0.5 C higher than that of 5S RNAs from B. subtilis and Escherichia coli. Melting in 50% methanol and in formaldehyde indicate that both base stacking and helical regions are involved in the slightly increased thermal stability of B. stearotherm...

journal_title：Journal of bacteriology

authors： Varricchio F,Marotta CA

更新日期：1976-03-01 00:00:00

abstract：:Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and ...

journal_title：Journal of bacteriology

authors： Levy SB

更新日期：1971-10-01 00:00:00

abstract：:The type IV secretion system encoded by the virB operon is required for full virulence of Brucella sp., and the present study links the RNA chaperone Hfq to wild-type expression of virB in Brucella abortus 2308. Studies employing virB-lacZ fusions, quantitative reverse transcription-PCR, and immunoblot analysis showed...

journal_title：Journal of bacteriology

authors： Caswell CC,Gaines JM,Roop RM 2nd

更新日期：2012-01-01 00:00:00

abstract：:We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...

journal_title：Journal of bacteriology

authors： Newton SM,Klebba PE,Michel V,Hofnung M,Charbit A

更新日期：1996-06-01 00:00:00

abstract：:Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporul...

journal_title：Journal of bacteriology

authors： Geng Y,de Keyzer J,Scheffers DJ,Driessen AJ

更新日期：2014-04-01 00:00:00

abstract：:The spores of Bacillus subtilis show remarkable resistance to many environmental stresses, due in part to the presence of an outer proteinaceous structure known as the spore coat. GerQ is a spore coat protein essential for the presence of CwlJ, an enzyme involved in the hydrolysis of the cortex during spore germinatio...

journal_title：Journal of bacteriology

authors： Ragkousi K,Setlow P

更新日期：2004-09-01 00:00:00

abstract：:In bacteria, the 4-amino-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine is synthesized from 5-aminoimidazole ribotide (AIR), a branch point metabolite of purine and thiamine biosynthesis. ThiC is a member of the radical S-adenosylmethionine (AdoMet) superfamily and catalyzes the complex chemical rearrangeme...

journal_title：Journal of bacteriology

authors： Palmer LD,Dougherty MJ,Downs DM

更新日期：2012-11-01 00:00:00

abstract：:The gene coding for the metabolically stable 6S RNA of Escherichia coli has been cloned, sequenced, and partially characterized in expression analyses. The DNA sequence results confirm the accuracy of the previously established RNA sequence and, with genomic hybridization data, reveal that there is only one copy of th...

journal_title：Journal of bacteriology

authors： Hsu LM,Zagorski J,Wang Z,Fournier MJ

更新日期：1985-03-01 00:00:00

abstract：:lisRK encodes a two-component regulatory system in the food pathogen Listeria monocytogenes LO28. Following identification of the operon in an acid-tolerant Tn917 mutant, a deletion in the histidine kinase component was shown to result in a growth phase variation in acid tolerance, an ability to grow in high ethanol c...

journal_title：Journal of bacteriology

authors： Cotter PD,Emerson N,Gahan CG,Hill C

更新日期：1999-11-01 00:00:00

abstract：:A homologue of the grmA spore germination gene of Bacillus megaterium and of a NaH-antiporter gene (napA) of Enterococcus hirae has been identified in Bacillus cereus 569 (ATCC 10876). The putative protein product has 58 and 43% amino acid identity with GrmA and NapA, respectively. Insertional inactivation of this B. ...

journal_title：Journal of bacteriology

authors： Thackray PD,Behravan J,Southworth TW,Moir A

更新日期：2001-01-01 00:00:00

abstract：:Nishida, S. (Kanazawa University, Kanazawa, Japan), and G. Nakagawara. Isolation of toxigenic strains of Clostridium novyi from soil. J. Bacteriol. 88:1636-1640. 1964.-The cultural conditions for toxin production by Clostridium novyi were investigated, and the optimal constituents of a proper medium were determined. A...

journal_title：Journal of bacteriology

authors： NISHIDA S,NAKAGAWARA G

更新日期：1964-12-01 00:00:00

abstract：:O antigen is the major cell surface antigen of gram-negative bacteria, and the genes responsible for its synthesis are located in a single gene cluster. The wzx (rbfX) gene, which is characteristic of the major class of O-antigen gene clusters, encodes a hydrophobic protein with 12 potential transmembrane segments. We...

journal_title：Journal of bacteriology

authors： Liu D,Cole RA,Reeves PR

更新日期：1996-04-01 00:00:00

abstract：:Twenty-six FliF monomers assemble into the MS ring, a central motor component of the bacterial flagellum that anchors the structure in the inner membrane. Approximately 100 amino acids at the C terminus of FliF are exposed to the cytoplasm and, through the interaction with the FliG switch protein, a component of the f...

journal_title：Journal of bacteriology

authors： Grünenfelder B,Gehrig S,Jenal U

更新日期：2003-03-01 00:00:00

abstract：:Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilizatio...

journal_title：Journal of bacteriology

authors： Joshua CJ,Dahl R,Benke PI,Keasling JD

更新日期：2011-03-01 00:00:00

abstract：:The phage 21 holin, S(21), forms small membrane holes that depolarize the membrane and is designated as a pinholin, as opposed to large-hole-forming holins, like S(lambda). Pinholins require secreted SAR endolysins, a pairing that may represent an intermediate in the evolution of canonical holin-endolysin systems. ...

journal_title：Journal of bacteriology

authors： Park T,Struck DK,Dankenbring CA,Young R

更新日期：2007-12-01 00:00:00

abstract：:The fim operon of Salmonella enterica serovar Typhimurium encodes type 1 fimbriae. The expression of fim is controlled in response to environmental signals through a complex regulatory cascade involving the proteins FimW, FimY, and FimZ and a genetic locus, fimU, that encodes a rare arginine tRNA. We discovered that a...

journal_title：Journal of bacteriology

authors： McFarland KA,Lucchini S,Hinton JC,Dorman CJ

更新日期：2008-01-01 00:00:00