Absence of diauxie during simultaneous utilization of glucose and Xylose by Sulfolobus acidocaldarius.

Abstract:

:Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilization. The organism utilized a mixture of 1 g/liter of each sugar simultaneously with a specific growth rate of 0.079 h(-1) and showed no preference for the order in which it utilized each sugar. The organism grew faster on 2 g/liter xylose (0.074 h(-1)) as the sole carbon source than on an equal amount of glucose (0.022 h(-1)). When grown on a mixture of the two carbon sources, the growth rate of the organism increased from 0.052 h(-1) to 0.085 h(-1) as the ratio of xylose to glucose increased from 0.25 to 4. S. acidocaldarius appeared to utilize a mixture of glucose and xylose at a rate roughly proportional to their concentrations in the medium, resulting in complete utilization of both sugars at about the same time. Gene expression in cells grown on xylose alone was very similar to that in cells grown on a mixture of xylose and glucose and substantially different from that in cells grown on glucose alone. The mechanism by which the organism utilized a mixture of sugars has yet to be elucidated.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Joshua CJ,Dahl R,Benke PI,Keasling JD

doi

10.1128/JB.01219-10

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

1293-301

issue

6

eissn

0021-9193

issn

1098-5530

pii

JB.01219-10

journal_volume

193

pub_type

杂志文章
  • Draft genome sequence of probiotic strain Lactobacillus rhamnosus R0011.

    abstract::Lactobacillus rhamnosus R0011 is a commercially available probiotic that is widely used in human dietary supplements and pharmaceutical products. We prepared a draft genome sequence consisting of 10 contigs totaling 2,900,620 bases and a G+C content of 46.7% for this strain. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06584-11

    authors: Tompkins TA,Barreau G,de Carvalho VG

    更新日期:2012-02-01 00:00:00

  • Replication Rapidly Recovers and Continues in the Presence of Hydroxyurea in Escherichia coli.

    abstract::In both prokaryotes and eukaryotes, hydroxyurea is suggested to inhibit DNA replication by inactivating ribonucleotide reductase and depleting deoxyribonucleoside triphosphate pools. In this study, we show that the inhibition of replication in Escherichia coli is transient even at concentrations of 0.1 M hydroxyurea a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00713-17

    authors: Nazaretyan SA,Savic N,Sadek M,Hackert BJ,Courcelle J,Courcelle CT

    更新日期:2018-02-23 00:00:00

  • The F-plasmid TraI protein contains three functional domains required for conjugative DNA strand transfer.

    abstract::The F-plasmid-encoded TraI protein, also known as DNA helicase I, is a bifunctional protein required for conjugative DNA transfer. The enzyme catalyzes two distinct but functionally related reactions required for the DNA processing events associated with conjugation: the site- and strand-specific transesterification (...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.2.697-706.2005

    authors: Matson SW,Ragonese H

    更新日期:2005-01-01 00:00:00

  • Identification of a novel arsenite oxidase gene, arxA, in the haloalkaliphilic, arsenite-oxidizing bacterium Alkalilimnicola ehrlichii strain MLHE-1.

    abstract::Although arsenic is highly toxic to most organisms, certain prokaryotes are known to grow on and respire toxic metalloids of arsenic (i.e., arsenate and arsenite). Two enzymes are known to be required for this arsenic-based metabolism: (i) the arsenate respiratory reductase (ArrA) and (ii) arsenite oxidase (AoxB). Bot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00244-10

    authors: Zargar K,Hoeft S,Oremland R,Saltikov CW

    更新日期:2010-07-01 00:00:00

  • Activation of ribulose 1,5-bisphosphate carboxylase from Rhodopseudomonas sphaeroides: probable role of the small subunit.

    abstract::The activation properties of the form I and form II ribulose 1,5-bisphosphate carboxylases from Rhodopseudomonas sphaeroides were examined. Both enzymes have a requirement of Mg2+ for optimal activity. Mn2+, Ni2+, and Co2+ can also support activity of the form I enzyme, whereas only Mn2+ can substitute for Mg2+ with t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.1023-1027.1979

    authors: Gibson JL,Tabita FR

    更新日期:1979-12-01 00:00:00

  • Overexpression of tnaC of Escherichia coli inhibits growth by depleting tRNA2Pro availability.

    abstract::Transcription of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. Induction results from ribosome stalling after translation of tnaC, the coding region for a 24-residue leader peptide. The last sense codon of tnaC, proline co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.5.1892-1898.2006

    authors: Gong M,Gong F,Yanofsky C

    更新日期:2006-03-01 00:00:00

  • Identification and function of the pdxY gene, which encodes a novel pyridoxal kinase involved in the salvage pathway of pyridoxal 5'-phosphate biosynthesis in Escherichia coli K-12.

    abstract::pdrK encodes a pyridoxine (PN)/pyridoxal (PL)/pyridoxamine (PM) kinase thought to function in the salvage pathway of pyridoxal 5'-phosphate (PLP) coenzyme biosynthesis. The observation that pdxK null mutants still contain PL kinase activity led to the hypothesis that Escherichia coli K-12 contains at least one other B...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.7.1814-1821.1998

    authors: Yang Y,Tsui HC,Man TK,Winkler ME

    更新日期:1998-04-01 00:00:00

  • Antisense RNA regulation by stable complex formation in the Enterococcus faecalis plasmid pAD1 par addiction system.

    abstract::The par stability determinant, encoded by the Enterococcus faecalis plasmid pAD1, is the only antisense RNA regulated postsegregational killing system identified in gram-positive bacteria. Because of the unique organization of the par locus, the par antisense RNA, RNA II, binds to its target, RNA I, at relatively smal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.19.6400-6408.2004

    authors: Weaver KE,Ehli EA,Nelson JS,Patel S

    更新日期:2004-10-01 00:00:00

  • Change in direction of flagellar rotation in Escherichia coli mediated by acetate kinase.

    abstract::Strains of Escherichia coli lacking all cytoplasmic chemotaxis proteins except CheY swim smoothly under most conditions. However, they tumble when exposed to acetate. Acetate coenzyme A synthetase (EC 6.2.1.1) was thought to be essential for this response. New evidence suggests that the tumbling is mediated instead by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.10.3236-3239.1993

    authors: Dailey FE,Berg HC

    更新日期:1993-05-01 00:00:00

  • Insertional inactivation of genes encoding components of the sodium-type flagellar motor and switch of Vibrio parahaemolyticus.

    abstract::Vibrio parahaemolyticus possesses two types of flagella, polar and lateral, powered by distinct energy sources, which are derived from the sodium and proton motive forces, respectively. Although proton-powered flagella in Escherichia coli and Salmonella enterica serovar Typhimurium have been extensively studied, the m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.4.1035-1045.2000

    authors: Boles BR,McCarter LL

    更新日期:2000-02-01 00:00:00

  • The interaction of T4 endonuclease V E23Q mutant with thymine dimer- and tetrahydrofuran-containing DNA.

    abstract::The interaction between endonuclease V, the cyclobutane pyrimidine dimer-specific N-glycosylase/abasic lyase from bacteriophage T4, and DNA was investigated by DNase I footprinting methods. The catalytically inactive mutant E23Q was found to interact with a smaller region of DNA at the abasic site analog, tetrahydrofu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.17.5166-5168.1995

    authors: Latham KA,Manuel RC,Lloyd RS

    更新日期:1995-09-01 00:00:00

  • Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli.

    abstract::DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination. Because the phenotypes of individual uvrD alleles vary significantly, we constructed deletion-insertion mutations in the uvrD gene to determine the phenotype of cells lacking DNA helicase II. Deletion muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2569-2575.1991

    authors: Washburn BK,Kushner SR

    更新日期:1991-04-01 00:00:00

  • Taxonomy of marine bacteria: Beneckea parahaemolytica and Beneckea alginolytica.

    abstract::A collection of 169 strains, including 91 obtained from cases of gastroenteritis and 41 from localized tissue infections and infections of the eye and ear, was submitted to an extensive nutritional, physiological, and morphological characterization. The nutritional and physiological data obtained from these strains, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.3.1144-1155.1973

    authors: Baumann P,Baumann L,Reichelt JL

    更新日期:1973-03-01 00:00:00

  • Quantification of group A colicin import sites.

    abstract::Pore-forming colicins are soluble bacteriocins which form voltage-gated ion channels in the inner membrane of Escherichia coli. To reach their target, these colicins first bind to a receptor located on the outer membrane and then are translocated through the envelope. Colicins are subdivided into two groups according ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.17.4935-4939.1995

    authors: Duché D,Letellier L,Géli V,Bénédetti H,Baty D

    更新日期:1995-09-01 00:00:00

  • Regulation of expression of the Escherichia coli K-12 mtr gene by TyrR protein and Trp repressor.

    abstract::The Escherichia coli K-12 mtr gene, which encodes a tryptophan-specific permease, was cloned, and its nucleotide sequence was determined. The precise location of the mtr gene at 69 min on the E. coli chromosome was determined. The mtr gene product was identified as a 414-amino-acid residue protein with a calculated mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.13.4133-4143.1991

    authors: Sarsero JP,Wookey PJ,Pittard AJ

    更新日期:1991-07-01 00:00:00

  • Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.

    abstract::The expression of the pur operon, which encodes enzymes of the purine biosynthetic pathway in Bacillus subtilis, is subject to control by the purR gene product (PurR) and phosphoribosylpyrophosphate. This control is also exerted on the purA and purR genes. A consensus sequence for the binding of PurR, named the PurBox...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.21.6175-6183.2001

    authors: Saxild HH,Brunstedt K,Nielsen KI,Jarmer H,Nygaard P

    更新日期:2001-11-01 00:00:00

  • P1 ParB domain structure includes two independent multimerization domains.

    abstract::ParB is one of two P1-encoded proteins that are required for active partition of the P1 prophage in Escherichia coli. To probe the native domain structure of ParB, we performed limited proteolytic digestions of full-length ParB, as well as of several N-terminal and C-terminal deletion fragments of ParB. The C-terminal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.19.5898-5908.1999

    authors: Surtees JA,Funnell BE

    更新日期:1999-10-01 00:00:00

  • Thermolability of Malic Dehydrogenase from the Obligate Psychrophile Vibrio marinus.

    abstract::Langridge, Patricia (Oregon State University, Corvallis), and Richard Y. Morita. Thermolability of malic dehydrogenase from the obligate psychrophile Vibrio marinus. J. Bacteriol. 92:418-423. 1966.-The thermolability of malic dehydrogenase in whole cells of Vibrio marinus MP-1 grown at 15 C was compared with that of c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.2.418-423.1966

    authors: Langridge P,Morita RY

    更新日期:1966-08-01 00:00:00

  • Properties of Bacillus megaterium temperature-sensitive germination mutants.

    abstract::Bacillus megaterium mutants JV-9 and JV-10 are temperature sensitive for initiation of spore germination. At 46 C, they did not lose heat resistance, dipicolinic acid, or absorbance, indicating that the temperature-sensitive blocks are very early in the sequence of initiation reactions. Strain JV-9 was temperature sen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.1.197-203.1975

    authors: Vary JC

    更新日期:1975-01-01 00:00:00

  • Localization of acyl carrier protein in Escherichia coli.

    abstract::Acyl carrier protein was localized by immunoelectron microscopy in the cytoplasm of Escherichia coli. These data are inconsistent with the previous report of an association between acyl carrier protein and the inner membrane (H. Van den Bosch, J.R. Williamson, and P.R. Vagelos, Nature [London] 228:338-341, 1970). More...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.5-8.1985

    authors: Jackowski S,Edwards HH,Davis D,Rock CO

    更新日期:1985-04-01 00:00:00

  • Eubacterial components similar to small nuclear ribonucleoproteins: identification of immunoprecipitable proteins and capped RNAs in a cyanobacterium and a gram-positive eubacterium.

    abstract::Small nuclear ribonucleoprotein (snRNP) particles play an important role in the processing of pre-mRNA. snRNPs have been identified immunologically in a variety of cells, but none have ever been observed in prokaryotic systems. This report provides the first evidence for the presence of snRNP-like components in two ty...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.7.1871-1878.1993

    authors: Kovacs SA,O'Neil J,Watcharapijarn J,Moe-Kirvan C,Vijay S,Silva V

    更新日期:1993-04-01 00:00:00

  • Simple method for identification of plasmid-coded proteins.

    abstract::Proteins encoded by plasmid DNA are specifically labeled in UV-irradiated cells of Escherichia coli carrying recA and uvrA mutations because extensive degradation of the chromosome DNA occurs concurrently with amplification of plasmid DNA. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.692-693.1979

    authors: Sancar A,Hack AM,Rupp WD

    更新日期:1979-01-01 00:00:00

  • Efficiency of thymidine incorporation in Escherichia coli B-r as a function of growth rate.

    abstract::Short-term labeling with radioactive thymidine led to inaccurate estimates of deoxyribonucleic acid synthesis at some growth rates in steady-state cultures of Escherichia coli B/r. Estimates were corrected in chemostat cultures by adding adenosine, a known inhibitor of thymidine phosphorylase. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.3.1208-1210.1972

    authors: Kubitschek HE,Valdez D,Freedman ML

    更新日期:1972-06-01 00:00:00

  • Light-induced, carrier-mediated transport of tetracycline by Rhodopseudomonas sphaeroides.

    abstract::Tetracycline accumulation by the phototrophic bacterium Rhodopseudomonas sphaeroides has been studied, using the fluorescence properties of the antibiotic and measuring uptake of [7- 3H]tetracycline. Accumulation was carrier mediated, with a Km of approximately 300 micronM. Efflux also appeared to be carried mediated,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.3.678-683.1979

    authors: Weckesser J,Magnuson JA

    更新日期:1979-06-01 00:00:00

  • The Rnf Complex Is an Energy-Coupled Transhydrogenase Essential To Reversibly Link Cellular NADH and Ferredoxin Pools in the Acetogen Acetobacterium woodii.

    abstract::The Rnf complex is a respiratory enzyme that catalyzes the oxidation of reduced ferredoxin to the reduction of NAD+, and the negative free energy change of this reaction is used to generate a transmembrane ion gradient. In one class of anaerobic acetogenic bacteria, the Rnf complex is believed to be essential for ener...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00357-18

    authors: Westphal L,Wiechmann A,Baker J,Minton NP,Müller V

    更新日期:2018-10-10 00:00:00

  • Stepwise activity of ClpY (HslU) mutants in the processive degradation of Escherichia coli ClpYQ (HslUV) protease substrates.

    abstract::In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease composed of ClpY (HslU), an ATPase with unfolding activity, and ClpQ (HslV), a peptidase. In the ClpYQ proteolytic complex, the hexameric rings of ClpY (HslU) are responsible for protein recognition, unfolding, and translocation into the prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05128-11

    authors: Hsieh FC,Chen CT,Weng YT,Peng SS,Chen YC,Huang LY,Hu HT,Wu YL,Lin NC,Wu WF

    更新日期:2011-10-01 00:00:00

  • Lytic effects of staphylococcal alpha-toxin and delta-hemolysin.

    abstract::Several preparations of staphylococcal alpha-toxin and delta-lysin were studied in order to compare hemolytic activity with capacity to lyse bacterial protoplasts. delta-Lysin in relatively low concentration lysed protoplasts of Sarcina lutea, protoplasts of Streptococcus faecalis, and spheroplasts of Escherichia coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.2.487-491.1968

    authors: Bernheimer AW,Avigad LS,Grushoff P

    更新日期:1968-08-01 00:00:00

  • Global gene expression analysis of the heat shock response in the phytopathogen Xylella fastidiosa.

    abstract::Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fastidiosa stress responses. One of the better characterized stress responses in bacteria is the heat shock response, which ind...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00182-06

    authors: Koide T,Vêncio RZ,Gomes SL

    更新日期:2006-08-01 00:00:00

  • Identification of a global repressor gene, rsmA, of Erwinia carotovora subsp. carotovora that controls extracellular enzymes, N-(3-oxohexanoyl)-L-homoserine lactone, and pathogenicity in soft-rotting Erwinia spp.

    abstract::The production of extracellular enzymes such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt) is activated by the cell density (quorum)-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (HSL); plant signals; and aep genes during postexponential growth of Erwinia carotovora subs...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.17.5108-5115.1995

    authors: Cui Y,Chatterjee A,Liu Y,Dumenyo CK,Chatterjee AK

    更新日期:1995-09-01 00:00:00

  • Francisella novicida CRISPR-Cas Systems Can Functionally Complement Each Other in DNA Defense while Providing Target Flexibility.

    abstract::CRISPR-Cas systems are prokaryotic adaptive immune systems that facilitate protection of bacteria and archaea against infection by external mobile genetic elements. The model pathogen Francisella novicida encodes a CRISPR-Cas12a (FnoCas12a) system and a CRISPR-Cas9 (FnoCas9) system, the latter of which has an addition...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00670-19

    authors: Ratner HK,Weiss DS

    更新日期:2020-05-27 00:00:00