Abstract:
:Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilization. The organism utilized a mixture of 1 g/liter of each sugar simultaneously with a specific growth rate of 0.079 h(-1) and showed no preference for the order in which it utilized each sugar. The organism grew faster on 2 g/liter xylose (0.074 h(-1)) as the sole carbon source than on an equal amount of glucose (0.022 h(-1)). When grown on a mixture of the two carbon sources, the growth rate of the organism increased from 0.052 h(-1) to 0.085 h(-1) as the ratio of xylose to glucose increased from 0.25 to 4. S. acidocaldarius appeared to utilize a mixture of glucose and xylose at a rate roughly proportional to their concentrations in the medium, resulting in complete utilization of both sugars at about the same time. Gene expression in cells grown on xylose alone was very similar to that in cells grown on a mixture of xylose and glucose and substantially different from that in cells grown on glucose alone. The mechanism by which the organism utilized a mixture of sugars has yet to be elucidated.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Joshua CJ,Dahl R,Benke PI,Keasling JDdoi
10.1128/JB.01219-10subject
Has Abstractpub_date
2011-03-01 00:00:00pages
1293-301issue
6eissn
0021-9193issn
1098-5530pii
JB.01219-10journal_volume
193pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:2001-11-01 00:00:00
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更新日期:1993-04-01 00:00:00
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更新日期:2006-08-01 00:00:00
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更新日期:1995-09-01 00:00:00
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更新日期:2020-05-27 00:00:00