Overexpression of tnaC of Escherichia coli inhibits growth by depleting tRNA2Pro availability.

abstract：:An open reading frame (slr0899) on the genome of Synechocystis sp. strain PCC 6803 encodes a polypeptide of 149 amino acid residues, the sequence of which is 40% identical to that of cyanase from Escherichia coli. Introduction into a cyanase-deficient E. coli strain of a plasmid-borne slr0899 resulted in expression of...

journal_title：Journal of bacteriology

authors： Harano Y,Suzuki I,Maeda S,Kaneko T,Tabata S,Omata T

更新日期：1997-09-01 00:00:00

abstract：:Micromonospora strains have been isolated from diverse niches, including soil, water, and marine sediments and root nodules of diverse symbiotic plants. In this work, we report the genome sequence of Micromonospora lupini Lupac 08 isolated from root nodules of the wild legume Lupinus angustifolious. ...

journal_title：Journal of bacteriology

authors： Alonso-Vega P,Normand P,Bacigalupe R,Pujic P,Lajus A,Vallenet D,Carro L,Coll P,Trujillo ME

更新日期：2012-08-01 00:00:00

abstract：:The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess Delta(1)-pyrroline-5-carboxylate, the immediate precursor of proline, is added to a culture. In proline auxotrophs block...

journal_title：Journal of bacteriology

authors： Berg CM,Rossi JJ

更新日期：1974-06-01 00:00:00

abstract：:Sulfate transport capacity was not regulated by cysteine, methionine, or glutathione in Pseudomonas halodurans, but growth on sulfate or thiosulfate suppressed transport. Subsequent sulfur starvation of cultures grown on all sulfur sources except glutathione stimulated uptake. Only methionine failed to regulate sulfat...

journal_title：Journal of bacteriology

authors： Cuhel RL,Taylor CD,Jannasch HW

更新日期：1981-08-01 00:00:00

abstract：:Escherichia coli cells harboring a non-attenuated trp-lac operon fusion were used to evaluate the effects of indolmycin on the initiation of transcription at the trp promoter. Indolmycin caused repression in trpR+ strains and in trpR deletion mutants, although higher effector concentrations were required in the latter...

journal_title：Journal of bacteriology

authors： Bogosian G,Haydock PV,Somerville RL

更新日期：1983-02-01 00:00:00

abstract：:The vir locus of Bordetella pertussis apparently encodes a trans-acting positive regulator that is required for the coordinate expression of genes associated with virulence: pertussis toxin, filamentous hemagglutinin (FHA), hemolysin, and adenylate cyclase toxin. DNA clones of vir and of genes required for the synthes...

journal_title：Journal of bacteriology

authors： Stibitz S,Weiss AA,Falkow S

更新日期：1988-07-01 00:00:00

abstract：:Rhodococcus equi is a facultative intracellular pathogen which proliferates rapidly in both manure-enriched soil and alveolar macrophages. Although both environments are characterized by extremely low concentrations of free iron, very little is known regarding the strategies employed by R. equi to thrive under these c...

journal_title：Journal of bacteriology

authors： Miranda-Casoluengo R,Duffy PS,O'Connell EP,Graham BJ,Mangan MW,Prescott JF,Meijer WG

更新日期：2005-05-01 00:00:00

abstract：:Integrative and conjugative elements (ICEs), also known as conjugative transposons, are mobile genetic elements that can transfer from one bacterial cell to another by conjugation. ICEBs1 is integrated into the trnS-leu2 gene of Bacillus subtilis and is regulated by the SOS response and the RapI-PhrI cell-cell peptide...

journal_title：Journal of bacteriology

authors： Lee CA,Grossman AD

更新日期：2007-10-01 00:00:00

abstract：:We have evaluated the use of [1,2-13C2]propionate for the analysis of propionic acid metabolism, based on the ability to distinguish between the methylcitrate and methylmalonate pathways. Studies using propionate-adapted Escherichia coli MG1655 cells were performed. Preservation of the 13C-13C-12C carbon skeleton in l...

journal_title：Journal of bacteriology

authors： London RE,Allen DL,Gabel SA,DeRose EF

更新日期：1999-06-01 00:00:00

abstract：:Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...

journal_title：Journal of bacteriology

authors： Winkler ME,Schoenlein PV,Ross CM,Barrett JT,Ely B

更新日期：1984-10-01 00:00:00

abstract：:The process of flagellar assembly in Salmonella typhimurium was investigated by using temperature-sensitive mutants. The mutants were grown at the restrictive temperature and then at the permissive temperature, with radiolabel supplied in the first phase of the experiment and not the second, or vice versa. Flagellar h...

journal_title：Journal of bacteriology

authors： Jones CJ,Macnab RM

更新日期：1990-03-01 00:00:00

abstract：:Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical...

journal_title：Journal of bacteriology

authors： Amikam D,Kuhn J

更新日期：1987-05-01 00:00:00

abstract：:Accumulation of l-alpha-glycerophosphate, in cells of Bacillus subtilis mutants lacking the nicotinamide adenine dinucleotide-independent glycerophosphate dehydrogenase activity, suppresses both growth and sporulation. After growth has stopped, the cells slowly develop one and later more asymmetric septa that are thic...

journal_title：Journal of bacteriology

authors： O YK,Freese EB,Freese E

更新日期：1973-02-01 00:00:00

abstract：:Biofilms are structured multicellular communities of bacteria that form through a developmental process. In standing culture, undomesticated strains of Bacillus subtilis produce a floating biofilm, called a pellicle, with a distinct macroscopic architecture. Here we report on a comprehensive analysis of B. subtilis pe...

journal_title：Journal of bacteriology

authors： Kobayashi K

更新日期：2007-07-01 00:00:00

abstract：:Alginates are polysaccharides composed of 1-4-linked beta-D-mannuronic acid and alpha-L-guluronic acid. The polymer can be degraded by alginate lyases, which cleave the polysaccharide using a beta-elimination reaction. Two such lyases have previously been identified in the soil bacterium Azotobacter vinelandii, as fol...

journal_title：Journal of bacteriology

authors： Gimmestad M,Ertesvåg H,Heggeset TM,Aarstad O,Svanem BI,Valla S

更新日期：2009-08-01 00:00:00

abstract：:It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle....

journal_title：Journal of bacteriology

authors： Sikora AE,Zielke R,Datta K,Maddock JR

更新日期：2006-02-01 00:00:00

abstract：:The Staphylococcus aureus SarA global regulator controls the expression of numerous virulence genes, often in conjunction with the agr quorum-sensing system and its effector RNA, RNAIII. In the present study, we have examined the role of both SarA and RNAIII on the regulation of the promoter of tst, encoding staphyloc...

journal_title：Journal of bacteriology

authors： Andrey DO,Renzoni A,Monod A,Lew DP,Cheung AL,Kelley WL

更新日期：2010-11-01 00:00:00

abstract：:Exponentially growing populations of Bacillus subtilis contain two morphologically and functionally distinct cell types: motile individuals and nonmotile multicellular chains. Motility differentiation arises because RNA polymerase and the alternative sigma factor sigma(D) activate expression of flagellin in a subpopul...

journal_title：Journal of bacteriology

authors： Chen R,Guttenplan SB,Blair KM,Kearns DB

更新日期：2009-09-01 00:00:00

abstract：:The mutB gene of Salmonella typhimurium is involved in a methylation-independent repair pathway specific for A/G or A/C mismatches and is the homolog of the Escherichia coli mutY gene. The mutB gene of S. typhimurium was cloned and sequenced. The isolated mutB clone reduced the mutation rate of the mutB mutant to wild...

journal_title：Journal of bacteriology

authors： Desiraju V,Shanabruch WG,Lu AL

更新日期：1993-01-01 00:00:00

abstract：:Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...

journal_title：Journal of bacteriology

authors： Zhao N,Bai Y,Zhao XQ,Yang ZY,Bai FW

更新日期：2012-12-01 00:00:00

abstract：:The highly purified lipoprotein of the outer membrane of Escherichia coli forms paracrystals. The ultrastructures of these paracrystals were examined by electron microscopy. The needle-shaped paracrystals show several different band patterns, depending on conditions of paracrystallization. Models are presented to expl...

journal_title：Journal of bacteriology

authors： DeMartini M,Inouye S,Inouye M

更新日期：1976-07-01 00:00:00

abstract：:Desulfosporosinus species are sulfate-reducing bacteria belonging to the Firmicutes. Their genomes will give insights into the genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments and able to degrade toluene (Desulfosporosinus youngiae), to reduce Fe(III) (Desulfosporosin...

journal_title：Journal of bacteriology

authors： Pester M,Brambilla E,Alazard D,Rattei T,Weinmaier T,Han J,Lucas S,Lapidus A,Cheng JF,Goodwin L,Pitluck S,Peters L,Ovchinnikova G,Teshima H,Detter JC,Han CS,Tapia R,Land ML,Hauser L,Kyrpides NC,Ivanova NN,Pagani

更新日期：2012-11-01 00:00:00

abstract：:The nitrogenase proteins from eight organisms have been highly purified, and a survey of their cross-reactions shows that the nitrogenase proteins from a wide variety of organisms can interact with one another. An active cross-reaction is the complementary functioning of the MoFe protein and the Fe protein from differ...

journal_title：Journal of bacteriology

authors： Emerich DW,Burris RH

更新日期：1978-06-01 00:00:00

abstract：:Lipoprotein biogenesis in Gram-negative bacteria occurs by a conserved pathway, each step of which is considered essential. In contrast to this model, LoVullo and colleagues demonstrate that the N-acyl transferase Lnt is not required in Francisella tularensis or Neisseria gonorrhoeae. This suggests the existence of a ...

journal_title：Journal of bacteriology

authors： Chahales P,Thanassi DG

更新日期：2015-05-01 00:00:00

abstract：:The double-stranded DNA bacteriophage PRD1 uses an IncP plasmid-encoded conjugal transfer complex as a receptor. Plasmid functions in the PRD1 life cycle are restricted to phage adsorption and DNA entry. A single phage structural protein, P2, located at the fivefold capsid vertices, is responsible for PRD1 attachment ...

journal_title：Journal of bacteriology

authors： Grahn AM,Caldentey J,Bamford JK,Bamford DH

更新日期：1999-11-01 00:00:00

abstract：:Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertio...

journal_title：Journal of bacteriology

authors： Kanipes MI,Papp-Szabo E,Guerry P,Monteiro MA

更新日期：2006-05-01 00:00:00

abstract：:The transduction efficiency of Tn551, a staphylococcal transposon coding for erythromycin resistance (ermB), was increased by a factor of about five in acceptor strains carrying the gene for methicillin resistance (mec), as compared with methicillin-sensitive strains. This enhancement was independent of the generalize...

journal_title：Journal of bacteriology

authors： Berger-Bächi B

更新日期：1983-04-01 00:00:00

abstract：:We previously elucidated the global transcriptional responses of Escherichia coli to the nitrosating agent S-nitrosoglutathione (GSNO) in both aerobic and anaerobic chemostats, demonstrated the expression of nitric oxide (NO)-protective mechanisms, and obtained evidence of critical thiol nitrosation. The present study...

journal_title：Journal of bacteriology

authors： Pullan ST,Gidley MD,Jones RA,Barrett J,Stevanin TM,Read RC,Green J,Poole RK

更新日期：2007-03-01 00:00:00

abstract：:Total synthesis of acetate from CO2 by Clostridium acidiurici during fermentations of hypoxanthine has been shown to involve synthesis of glycine from methylenetetrahydrofolate, CO2, and NH3. The glycine is converted to serine by the addition of methylenetetrahydrofolate, and the resulting serine is converted to pyruv...

journal_title：Journal of bacteriology

authors： Waber LJ,Wood HG

更新日期：1979-11-01 00:00:00

abstract：:Upon exposure to alkylating agents, Escherichia coli increases expression of aidB along with three genes (ada, alkA, and alkB) that encode DNA repair proteins. While the biological roles of the Ada, AlkA, and AlkB proteins have been defined, despite many efforts, the molecular functions of AidB remain largely unknown....

journal_title：Journal of bacteriology

authors： Rippa V,Amoresano A,Esposito C,Landini P,Volkert M,Duilio A

更新日期：2010-12-01 00:00:00