Abnormal septation and inhibition of sporulation by accumulation of L- -glycerophosphate in Bacillus subtilis mutants.

Abstract:

:Accumulation of l-alpha-glycerophosphate, in cells of Bacillus subtilis mutants lacking the nicotinamide adenine dinucleotide-independent glycerophosphate dehydrogenase activity, suppresses both growth and sporulation. After growth has stopped, the cells slowly develop one and later more asymmetric septa that are thicker than normal prespore septa and apparently contain too much cell wall material to allow further membrane development into forespores or spores. l-Malate prevents accumulation of glycerophosphate and restores sporulation of the mutant. Glucose or gluconate cannot resotre sporulation, because they still effect glycerophosphate accumulation via de novo synthesis. If that accumulation is blocked in a double mutant, which is unable to make glycerophosphate from or to metabolize it into Embden-Meyerhof compounds, then nonsuppressing amounts of glucose or gluconate can restore sporulation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

O YK,Freese EB,Freese E

doi

10.1128/JB.113.2.1034-1045.1973

subject

Has Abstract

pub_date

1973-02-01 00:00:00

pages

1034-45

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

113

pub_type

杂志文章
  • Recombination in the ompA gene but not the omcB gene of Chlamydia contributes to serovar-specific differences in tissue tropism, immune surveillance, and persistence of the organism.

    abstract::Sequences of the major outer membrane protein (MOMP) gene (ompA) and the outer membrane complex B protein gene (omcB) from Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci were analyzed for evidence of intragenic recombination and for linkage equilibrium. The Sawyer runs test, compatibility matrices...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.20.5997-6008.2001

    authors: Millman KL,Tavaré S,Dean D

    更新日期:2001-10-01 00:00:00

  • Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis.

    abstract::We determined the nucleotide sequence of a 1.9-kilobase fragment of Pseudomonas paucimobilis SYK6 chromosomal DNA that included genes encoding protocatechuate 4,5-dioxygenase, the enzyme responsible for the aromatic ring fission of protocatechuate. Two open reading frames of 417 and 906 base pairs were found that had ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2704-2709.1990

    authors: Noda Y,Nishikawa S,Shiozuka K,Kadokura H,Nakajima H,Yoda K,Katayama Y,Morohoshi N,Haraguchi T,Yamasaki M

    更新日期:1990-05-01 00:00:00

  • Solitary BioY proteins mediate biotin transport into recombinant Escherichia coli.

    abstract::Energy-coupling factor (ECF) transporters form a large group of vitamin uptake systems in prokaryotes. They are composed of highly diverse, substrate-specific, transmembrane proteins (S units), a ubiquitous transmembrane protein (T unit), and homo- or hetero-oligomeric ABC ATPases. Biotin transporters represent a spec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00350-13

    authors: Finkenwirth F,Kirsch F,Eitinger T

    更新日期:2013-09-01 00:00:00

  • ORGANIC REQUIREMENTS AND SYNTHETIC MEDIA FOR GROWTH OF THE MYXOMYCETE PHYSARUM POLYCEPHALUM.

    abstract::Daniel, John W. (University of Wisconsin, Madison), Karlee L. Babcock, Alice H. Sievert, and Harold P. Rusch. Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum. J. Bacteriol. 86:324-331. 1963.-An isolate of Physarum polycephalum was maintained in submersed pure culture in a co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.2.324-331.1963

    authors: DANIEL JW,BABCOCK KL,SIEVERT AH,RUSCH HP

    更新日期:1963-08-01 00:00:00

  • Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathway.

    abstract::We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, whi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.111-118.2002

    authors: Tian H,Beckwith J

    更新日期:2002-01-01 00:00:00

  • Structure and organization of the pel genes from Erwinia chrysanthemi EC16.

    abstract::The pelA and pelC genes from Erwinia chrysanthemi EC16 were sequenced and overexpressed in Escherichia coli cells. These genes and two others from the same strain that were characterized previously encode catalytically related pectate lyase proteins that are involved with the maceration and soft-rotting of plant tissu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.8.3468-3478.1988

    authors: Tamaki SJ,Gold S,Robeson M,Manulis S,Keen NT

    更新日期:1988-08-01 00:00:00

  • Environmental role of pathogenic traits in Vibrio cholerae.

    abstract::Vibrio cholerae is a natural inhabitant of aquatic ecosystems. Some strains of V. cholerae can colonize the human host and cause cholera, a profuse watery diarrhea. The major pathogenicity factors and virulence regulators of V. cholerae are either encoded in mobile genetic elements acquired in the environment (e.g. pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00795-17

    authors: Sakib SN,Reddi G,Almagro-Moreno S

    更新日期:2018-03-26 00:00:00

  • Genetic, molecular, and functional analysis of Streptococcus faecalis R plasmid pJH1.

    abstract::Streptococcus faecalis JH1 contains two conjugative plasmids, pJH1, an R plasmid that codes for resistance to kanamycin, streptomycin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Strain JH1 was used as an antibiotic resistance donor in conjugation experiments with two plasmid-free S. fae...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1094-1104.1983

    authors: Banai M,LeBlanc DJ

    更新日期:1983-09-01 00:00:00

  • Presence of a novel phosphopentomutase and a 2-deoxyribose 5-phosphate aldolase reveals a metabolic link between pentoses and central carbon metabolism in the hyperthermophilic archaeon Thermococcus kodakaraensis.

    abstract::Numerous bacteria and mammalian cells harbor two enzymes, phosphopentomutase (PPM) and 2-deoxyribose 5-phosphate aldolase (DERA), involved in the interconversion between nucleosides and central carbon metabolism. In this study, we have examined the presence of this metabolic link in the hyperthermophilic archaeon, The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.13.4185-4191.2004

    authors: Rashid N,Imanaka H,Fukui T,Atomi H,Imanaka T

    更新日期:2004-07-01 00:00:00

  • Sigma factor RpoS controls alkylresorcinol synthesis through ArpR, a LysR-type regulatory protein, during encystment of Azotobacter vinelandii.

    abstract::Azotobacter vinelandii is a bacterium which undergoes a differentiation process leading to the formation of metabolically dormant cysts. During the encystment process, A. vinelandii produces alkylresorcinol lipids (ARs) that replace the membrane phospholipids and are also components of the layers covering the cyst. Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01946-12

    authors: Romero Y,Moreno S,Guzmán J,Espín G,Segura D

    更新日期:2013-04-01 00:00:00

  • A new pathway for salvaging the coenzyme B12 precursor cobinamide in archaea requires cobinamide-phosphate synthase (CbiB) enzyme activity.

    abstract::The ability of archaea to salvage cobinamide has been under question because archaeal genomes lack orthologs to the bacterial nucleoside triphosphate:5'-deoxycobinamide kinase enzyme (cobU in Salmonella enterica). The latter activity is required for cobinamide salvaging in bacteria. This paper reports evidence that ar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.24.7193-7201.2003

    authors: Woodson JD,Zayas CL,Escalante-Semerena JC

    更新日期:2003-12-01 00:00:00

  • The davDT operon of Pseudomonas putida, involved in lysine catabolism, is induced in response to the pathway intermediate delta-aminovaleric acid.

    abstract::Pseudomonas putida KT2440 is a soil microorganism that attaches to seeds and efficiently colonizes the plant's rhizosphere. Lysine is one of the major compounds in root exudates, and P. putida KT2440 uses this amino acid as a source of carbon, nitrogen, and energy. Lysine is channeled to delta-aminovaleric acid and th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.11.3439-3446.2004

    authors: Revelles O,Espinosa-Urgel M,Molin S,Ramos JL

    更新日期:2004-06-01 00:00:00

  • Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis.

    abstract::The general stress regulon of Bacillus subtilis comprises approximately 200 genes and is under the control of the alternative sigma factor σ(B). The activation of σ(B) occurs in response to multiple physical stress stimuli as well as energy starvation conditions. The expression of the general stress proteins provides ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00528-12

    authors: Reder A,Höper D,Gerth U,Hecker M

    更新日期:2012-07-01 00:00:00

  • Assembly of the mitochondrial membrane system: isolation of nuclear and cytoplasmic mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions.

    abstract::A selection procedure is described which permits a large number of Saccharomyces cerevisiae mutants to be screened for specific lesions in mitochondrial respiratory enzymes and the adenosine triphosphatase. The method has been used to isolate nuclear mutant strains with specific lesions in coenzyme QH2-cytochrome c re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.826-831.1975

    authors: Tzagoloff A,Akai A,Needleman RB

    更新日期:1975-06-01 00:00:00

  • Changes in cyclic nucleotide levels and dimorphic transition in Candida albicans.

    abstract::The relationship between the levels of cyclic nucleotides and dimorphic transition in Candida albicans was examined. The results showed that cells of this pathogenic fungus contained both cyclic adenosine 3',5'-monophosphate (cAMP) and cyclic guanosine 3',5'-monophosphate (cGMP), the concentration of the latter being ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.3.1010-1014.1980

    authors: Niimi M,Niimi K,Tokunaga J,Nakayama H

    更新日期:1980-06-01 00:00:00

  • Use of hupS::lacZ gene fusion to study regulation of hydrogenase expression in Rhodobacter capsulatus: stimulation by H2.

    abstract::The Escherichia coli beta-galactosidase enzyme was used as a reporter molecule for genetic fusions in Rhodobacter capsulatus. DNA fragments that were from the upstream region of the hydrogenase structural operon hupSLM and contained 5' hupS sequences were fused in frame to a promoterless lacZ gene, yielding fusion pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.13.4258-4264.1992

    authors: Colbeau A,Vignais PM

    更新日期:1992-07-01 00:00:00

  • Escherichia coli F41 adhesin: genetic organization, nucleotide sequence, and homology with the K88 determinant.

    abstract::The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4890-4896.1988

    authors: Anderson DG,Moseley SL

    更新日期:1988-10-01 00:00:00

  • Selectivity among Anti-σ Factors by Mycobacterium tuberculosis ClpX Influences Intracellular Levels of Extracytoplasmic Function σ Factors.

    abstract::Extracytoplasmic function σ factors that are stress inducible are often sequestered in an inactive complex with a membrane-associated anti-σ factor. Mycobacterium tuberculosis membrane-associated anti-σ factors have a small, stable RNA gene A (ssrA)-like degron for targeted proteolysis. Interaction between the unfolda...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00748-18

    authors: Joshi AC,Kaur P,Nair RK,Lele DS,Nandicoori VK,Gopal B

    更新日期:2019-02-25 00:00:00

  • Disruption of VirB6 Paralogs in Anaplasma phagocytophilum Attenuates Its Growth.

    abstract::Many pathogenic bacteria translocate virulence factors into their eukaryotic hosts by means of type IV secretion systems (T4SS) spanning the inner and outer membranes. Genes encoding components of these systems have been identified within the order Rickettsiales based upon their sequence similarities to other prototyp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00301-20

    authors: Crosby FL,Munderloh UG,Nelson CM,Herron MJ,Lundgren AM,Xiao YP,Allred DR,Barbet AF

    更新日期:2020-11-04 00:00:00

  • Purification and properties of a hydrogenase from Desulfovibrio vulgaris.

    abstract::The soluble hydrogenase of Desulfovibrio vulgaris was purified and some of its properties are described. The molecular weight was determined for the enzyme by sedimentation equilibrium (45,400) and amino acid analysis (44,800). The hydrogenase appears to be a loosely coiled molecule or to have a high axial ratio, whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.1.249-258.1971

    authors: Haschke RH,Campbell LL

    更新日期:1971-01-01 00:00:00

  • Nitric oxide in chemostat-cultured Escherichia coli is sensed by Fnr and other global regulators: unaltered methionine biosynthesis indicates lack of S nitrosation.

    abstract::We previously elucidated the global transcriptional responses of Escherichia coli to the nitrosating agent S-nitrosoglutathione (GSNO) in both aerobic and anaerobic chemostats, demonstrated the expression of nitric oxide (NO)-protective mechanisms, and obtained evidence of critical thiol nitrosation. The present study...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01354-06

    authors: Pullan ST,Gidley MD,Jones RA,Barrett J,Stevanin TM,Read RC,Green J,Poole RK

    更新日期:2007-03-01 00:00:00

  • Mutations affecting the citrate-dependent iron uptake system in Escherichia coli.

    abstract::Isolation of six strains of Escherichia coli K-12 carrying mutations affecting the citrate-dependent iron uptake system is described. Genetic analysis of these mutants showed that the mutation affecting the citrate system are cluster together at about min 6 on the E. coli chromosome. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.3.1524-1526.1978

    authors: Woodrow GC,Langman L,Young IG,Gibson F

    更新日期:1978-03-01 00:00:00

  • Identification of a new gene, molR, essential for utilization of molybdate by Escherichia coli.

    abstract::A mutation in a new gene, molR, prevented the synthesis in Escherichia coli of molybdoenzymes, including the two formate dehydrogenase isoenzymes, nitrate reductase and trimethylamine-N-oxide reductase. This phenotype was suppressed by supplementing the media with molybdate. Thus, the molR mutant was phenotypically si...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.4.2079-2087.1990

    authors: Lee JH,Wendt JC,Shanmugam KT

    更新日期:1990-04-01 00:00:00

  • Interaction of native and mutant MecI repressors with sequences that regulate mecA, the gene encoding penicillin binding protein 2a in methicillin-resistant staphylococci.

    abstract::Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.8.2160-2166.1998

    authors: Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

    更新日期:1998-04-01 00:00:00

  • Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coli.

    abstract::We report on the cloning and characterization of the rfb gene cluster of the O75 lipopolysaccharide from a urinary tract isolate of Escherichia coli. Deletion cloning defined the minimum region of DNA that expressed the O75 antigen in E. coli host strains to be on a 12.4-kb insert. However, the E. coli strain expressi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5699-5704.1991

    authors: Batchelor RA,Haraguchi GE,Hull RA,Hull SI

    更新日期:1991-09-01 00:00:00

  • Disruption of a rhodaneselike gene results in cysteine auxotrophy in Saccharopolyspora erythraea.

    abstract::A 3,373-base-pair DNA segment from a clone fortuitously isolated from Saccharopolyspora erythraea by hybridization to an oligodeoxynucleotide probe was sequenced. Computer-assisted analysis of the nucleotide sequence reveals three closely linked Streptomyces open reading frames plus a fourth converging on the others. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.1.350-360.1990

    authors: Donadio S,Shafiee A,Hutchinson CR

    更新日期:1990-01-01 00:00:00

  • Chemotaxis in Borrelia burgdorferi.

    abstract::Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not been elucidated. In this study, we found that motility of B. burgdorferi required an environment similar to interstitial ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.2.231-235.1998

    authors: Shi W,Yang Z,Geng Y,Wolinsky LE,Lovett MA

    更新日期:1998-01-01 00:00:00

  • The pinholin of lambdoid phage 21: control of lysis by membrane depolarization.

    abstract::The phage 21 holin, S(21), forms small membrane holes that depolarize the membrane and is designated as a pinholin, as opposed to large-hole-forming holins, like S(lambda). Pinholins require secreted SAR endolysins, a pairing that may represent an intermediate in the evolution of canonical holin-endolysin systems. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00847-07

    authors: Park T,Struck DK,Dankenbring CA,Young R

    更新日期:2007-12-01 00:00:00

  • Galactose transport in Saccharomyces cerevisiae. I. Nonmetabolized sugars as substrates and inducers of the galactose transport system.

    abstract::The inducible galactose transport system in bakers' yeast carries out the facilitated diffusion of the nonmetabolized galactose analogues d-fucose and l-arabinose. This capacity depends on the activity of the Ga 2 gene. In some strains, d-fucose and l-arabinose are also gratuitous inducers. Mutants in which the induci...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1727-1731.1968

    authors: Cirillo VP

    更新日期:1968-05-01 00:00:00

  • Importance of the position of TYR R boxes for repression and activation of the tyrP and aroF genes in Escherichia coli.

    abstract::Tyrosine-mediated repression of aroF and tyrP was studied by inserting DNA sequences between the two adjacent TYR R boxes which, in each case, overlap the respective RNA polymerase binding sites of these genes. In both cases, repression was greatest when homologous regions of these two TYR R boxes were on the same fac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.16.5079-5085.1991

    authors: Andrews AE,Dickson B,Lawley B,Cobbett C,Pittard AJ

    更新日期:1991-08-01 00:00:00