Solitary BioY proteins mediate biotin transport into recombinant Escherichia coli.

abstract：:Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping, complementation with a cosmid clone, DNA sequencing, and gene disruption and found that 16 of the 18 dsp alleles map to the dif genes....

journal_title：Journal of bacteriology

authors： Lancero H,Brofft JE,Downard J,Birren BW,Nusbaum C,Naylor J,Shi W,Shimkets LJ

更新日期：2002-03-01 00:00:00

abstract：:The terminal enzyme of heme biosynthesis, ferrochelatase (EC 4.99.1.1), catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme. Prior to the present work, [2Fe-2S] clusters have been identified and characterized in animal ferrochelatases but not in plant or prokaryotic ferrochelatases. Herein...

journal_title：Journal of bacteriology

authors： Dailey TA,Dailey HA

更新日期：2002-05-01 00:00:00

abstract：:The relationship between the levels of cyclic nucleotides and dimorphic transition in Candida albicans was examined. The results showed that cells of this pathogenic fungus contained both cyclic adenosine 3',5'-monophosphate (cAMP) and cyclic guanosine 3',5'-monophosphate (cGMP), the concentration of the latter being ...

journal_title：Journal of bacteriology

authors： Niimi M,Niimi K,Tokunaga J,Nakayama H

更新日期：1980-06-01 00:00:00

abstract：:The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indica...

journal_title：Journal of bacteriology

authors： Schlindwein C,Giordano G,Santini CL,Mandrand MA

更新日期：1990-10-01 00:00:00

abstract：:Dehalococcoides species are key players in the anaerobic transformation of halogenated solvents at contaminated sites. Here, we analyze isotopologue distributions in amino acid pools from peptides of Dehalococcoides strain CBDB1 after incubation with (13)C-labeled acetate or bicarbonate as a carbon source. The resulti...

journal_title：Journal of bacteriology

authors： Marco-Urrea E,Seifert J,von Bergen M,Adrian L

更新日期：2012-08-01 00:00:00

abstract：:Divercin V41, a class IIa bacteriocin with strong antilisterial activity, is produced by Carnobacterium divergens V41. To express a recombinant version of divercin V41, we constructed a synthetic gene that encodes the mature divercin V41 peptide and then overexpressed the gene in pET-32b by using the T7 RNA polymerase...

journal_title：Journal of bacteriology

authors： Richard C,Drider D,Elmorjani K,Marion D,Prévost H

更新日期：2004-07-01 00:00:00

abstract：:In Escherichia coli, the frequencies of IS50 and Tn5 transposition are greater in Dam- cells than in isogenic Dam+ cells. IS50 transposition is increased approximately 1,000-fold and Tn5 transposition frequencies are increased about 5- to 10-fold in the absence of Dam methylation. However, in cells that are deficient ...

journal_title：Journal of bacteriology

authors： Makris JC,Nordmann PL,Reznikoff WS

更新日期：1990-03-01 00:00:00

abstract：:The chb operon of Escherichia coli is involved in the utilization of the β-glucosides chitobiose and cellobiose. The function of chbG (ydjC), the sixth open reading frame of the operon that codes for an evolutionarily conserved protein is unknown. We show that chbG encodes a monodeacetylase that is essential for growt...

journal_title：Journal of bacteriology

authors： Verma SC,Mahadevan S

更新日期：2012-09-01 00:00:00

abstract：:Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...

journal_title：Journal of bacteriology

authors： Wolfe AD,Cook TM,Hahn FE

更新日期：1971-12-01 00:00:00

abstract：:A series of mutations of the P1 plasmid prophage that lead to increased copy number was isolated and analyzed. The copy number of the mutants was elevated at least five- to eightfold relative to wild-type P1, as determined by single-cell resistance to antibiotics, activity of enzymes, content of superhelical DNA, and ...

journal_title：Journal of bacteriology

authors： Scott JR,Kropf MM,Padolsky L,Goodspeed JK,Davis R,Vapnek D

更新日期：1982-06-01 00:00:00

abstract：:Zymomonas mobilis is endowed with two isoenzymes of fermentative alcohol dehydrogenase, a zinc-containing enzyme (ADH I) and an iron-containing enzyme (ADH II). The activity of ADH I remains fully conserved, while ADH II activity decays when anaerobic cultures are shifted to aerobiosis. This differential response depe...

journal_title：Journal of bacteriology

authors： Tamarit J,Cabiscol E,Aguilar J,Ros J

更新日期：1997-02-01 00:00:00

abstract：:In a search for genes that produce hypersensitivity to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used. One of the mutant strains obtained was sensitive to Cd2+ and Zn2+. Sequence analysis showed that the TnphoA insertion was located in the dsbA gene coding for a periplasmic prote...

journal_title：Journal of bacteriology

authors： Rensing C,Mitra B,Rosen BP

更新日期：1997-04-01 00:00:00

abstract：:We have previously cloned the genes for synthesis of capsular polysaccharide (cps) and slime from Erwinia stewartii in cosmid pES2144. In this study, pES2144 was shown to complement 14 spontaneous cps mutants. These mutants were characterized by probing Southern blots of mutant genomic DNA with pES2144; insertions wer...

journal_title：Journal of bacteriology

authors： Dolph PJ,Majerczak DR,Coplin DL

更新日期：1988-02-01 00:00:00

abstract：:Mutations in a regulator gene (capR) that causes increased synthesis of capsular polysaccharide and derepressed synthesis of several enzymes involved in polysaccharide synthesis also derepress synthesis of guanosine diphosphate (GDP)-mannose pyrophosphorylase. In addition, a second mucoid mutation (capS, which maps se...

journal_title：Journal of bacteriology

authors： Lieberman MM,Markovitz A

更新日期：1970-03-01 00:00:00

abstract：:A comparison of the distribution of CO oxidation activity between soluble and particulate protein fractions obtained after disruption of CO-grown Rhodopseudomonas gelatinosa 1 by French pressure cell breakage and osmotic lysis of spheroplasts suggested that, in situ, the enzyme complex was associated with the cell mem...

journal_title：Journal of bacteriology

authors： Wakim BT,Uffen RL

更新日期：1983-01-01 00:00:00

abstract：:A nitrate-utilizing strain of marine bacteria was isolated in which luciferase was inducible by l-arginine. The induction was highly specific; structural analogues of arginine were ineffective, as were other natural amino acids. Several metabolites of arginine acted as weak inducers but did not affect the rate of indu...

journal_title：Journal of bacteriology

authors： Coffey JJ

更新日期：1967-11-01 00:00:00

abstract：:Sigma 54-dependent transcriptional activators such as Escherichia coli NtrC, Rhizobium meliloti NifA, and Rhizobium leguminosarum DctD share similar central and carboxy-terminal domains but differ in the structure and function of their amino-terminal domains. We have deleted the amino-terminal and carboxy-terminal dom...

journal_title：Journal of bacteriology

authors： Huala E,Stigter J,Ausubel FM

更新日期：1992-02-01 00:00:00

abstract：:Cells of Escherichia coli take up vitamin B(12) (cyano-cobalamin [CN-Cbl]) and iron chelates by use of sequential active transport processes. Transport of CN-Cbl across the outer membrane and its accumulation in the periplasm is mediated by the TonB-dependent transporter BtuB. Transport across the cytoplasmic membrane...

journal_title：Journal of bacteriology

authors： Cadieux N,Bradbeer C,Reeger-Schneider E,Köster W,Mohanty AK,Wiener MC,Kadner RJ

更新日期：2002-02-01 00:00:00

abstract：:Photobacterium leiognathi ATCC 25521 (the type strain and light-organ symbiont of ponyfish) is one of the few bacteria that produces a copper-zinc superoxide dismutase, termed bacteriocuprein. We enzymologically and immunologically characterized the bacteriocuprein superoxide dismutases in sonicates from the type stra...

journal_title：Journal of bacteriology

authors： Dunlap PV,Steinman HM

更新日期：1986-02-01 00:00:00

abstract：:Using representational difference analysis, we isolated novel meningococcal restriction-modification (R-M) systems. NmeBI, which is a homologue of the R-M system HgaI of Pasteurella volantium, was present in meningococci of the ET-5 complex and of lineage III. NmeAI was found in serogroup A, ET-37 complex, and cluster...

journal_title：Journal of bacteriology

authors： Claus H,Friedrich A,Frosch M,Vogel U

更新日期：2000-03-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 produces specialized cells for nitrogen fixation called heterocysts. Previous work showed that the group 2 sigma factor sigE (alr4249; previously called sigF) is upregulated in differentiating heterocysts 16 h after nitrogen step-down. We now show th...

journal_title：Journal of bacteriology

authors： Mella-Herrera RA,Neunuebel MR,Kumar K,Saha SK,Golden JW

更新日期：2011-04-01 00:00:00

abstract：:Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an e...

journal_title：Journal of bacteriology

authors： Helmstetter CE

更新日期：1974-10-01 00:00:00

abstract：:The effect of sublethal, midlethal, and lethal doses of total-body X irradiation on the fecal microbial population of the rat was studied. No changes were observed in animals receiving sublethal doses of X ray. Midlethal and lethal doses produced an increase in the numbers of fecal coliforms, staphylococci, streptococ...

journal_title：Journal of bacteriology

authors： Klainer AS,Gorbach S,Weinstein L

更新日期：1967-08-01 00:00:00

abstract：:The valyl-transfer ribonucleic acid (tRNA) synthetase of Escherichia coli strain NP2907, previously described as having an elevated K(m) for adenosine triphosphate and reduced stability in vitro compared to the wild type, was found to be conditionally thermolabile in vivo. The rate of inactivation of this enzyme at a ...

journal_title：Journal of bacteriology

authors： Anderson JJ,Neidhardt FC

更新日期：1972-01-01 00:00:00

abstract：:Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...

journal_title：Journal of bacteriology

authors： Jablonski PE,Ferry JG

更新日期：1991-04-01 00:00:00

abstract：:A study was made of how the catabolism of a carbon and energy source is affected by the biosynthetic demands of growing bacterial cells. Cultures of Aerobacter aerogenes in l-histidine medium were grown in a chemostat at rates determined by the supply of either sulfate or a required amino acid, l-arginine. It was disc...

journal_title：Journal of bacteriology

authors： Jensen DE,Neidhardt FC

更新日期：1969-04-01 00:00:00

abstract：:Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated ou...

journal_title：Journal of bacteriology

authors： Bosch R,Moore ER,García-Valdés E,Pieper DH

更新日期：1999-04-01 00:00:00

abstract：:The DNA sequence of a 3.23-kilobase fragment of the Escherichia coli chromosome encoding biosynthetic arginine decarboxylase (ADC) was determined. This sequence contained the speA open reading frame (ORF) as well as partial speB and metK ORFs. The ADC ORF is 1,974 nucleotides long; the deduced polypeptide contains 658...

journal_title：Journal of bacteriology

authors： Moore RC,Boyle SM

更新日期：1990-08-01 00:00:00

abstract：:The cheF gene, which is involved in chemotaxis in Bacillus subtilis, has been cloned, expressed, and sequenced. This gene is contained in a 0.7-kilobase PstI DNA fragment that was isolated from a lambda Charon 4A B. subtilis chromosomal DNA library. This fragment was subcloned into the expression vector pSI-1 and show...

journal_title：Journal of bacteriology

authors： Ying CW,Ordal GW

更新日期：1989-03-01 00:00:00

abstract：:The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT ...

journal_title：Journal of bacteriology

authors： McBeth DL

更新日期：1989-02-01 00:00:00