Studies of intestinal microflora. VI. Effect of x irradiation on the fecal microflora of the rat.

abstract：:The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...

journal_title：Journal of bacteriology

authors： Khosla C,McDaniel R,Ebert-Khosla S,Torres R,Sherman DH,Bibb MJ,Hopwood DA

更新日期：1993-04-01 00:00:00

abstract：:Rhodopseudomonas capsulata required potassium (or rubidium or cesium as analogs of potassium) for growth. These cations were actively accumulated by the cells by a process following Michaelis-Menten saturation kinetics. The monovalent cation transport system had Km's of 0.2 mM K+, 0.5 mM Rb+, and 2.6 mM Cs+. The rates...

journal_title：Journal of bacteriology

authors： Jasper P

更新日期：1978-03-01 00:00:00

abstract：:Amber mutants of bacteriophage P1 were used to identify functions involved in late regulation of the P1 lytic growth cycle. A single function has been genetically identified to be involved in activation of the phage-specific late promoter sequence Ps. In vivo, P1 gene 10 amber mutants fail to trans activate a lacZ ope...

journal_title：Journal of bacteriology

authors： Lehnherr H,Guidolin A,Arber W

更新日期：1991-10-01 00:00:00

abstract：:Single spores of Saccharomyces cerevisiae were examined during germination and outgrowth by scanning electron and phase-contrast microscopy. Also determined were changes in cell weight and light absorbance, trehalose utilization, and synthesis of protein and KOH-soluble carbohydrates. These studies reveal that develop...

journal_title：Journal of bacteriology

authors： Rousseau P,Halvorson HO,Bulla LA Jr,St Julian G

更新日期：1972-03-01 00:00:00

abstract：:The heat shock response in Escherichia coli involves extensive induction of the heat shock proteins, with the concomitant suppression of the synthesis of the non-heat shock proteins. While the induction of the heat shock proteins has been shown to occur primarily at the transcriptional level, the suppression of non-he...

journal_title：Journal of bacteriology

authors： Henry MD,Yancey SD,Kushner SR

更新日期：1992-02-01 00:00:00

abstract：:Disrupted spheroplast preparations of Pseudomonas schuylkilliensis strain P contained fragments of cytoplasmic membrane and approximately 82% of the total cellular phospholipid. The protoplast-bursting factor (PB-factor), partially purified from pig pancreas, and a heat-treated pancreatic lipase fraction both inhibite...

journal_title：Journal of bacteriology

authors： Mizuno S,Maruo B

更新日期：1972-01-01 00:00:00

abstract：:The major facilitator superfamily includes a large collection of evolutionarily related proteins that have been implicated in the transport of a variety of solutes and metabolites across the membranes of organisms ranging from bacteria to humans. We have recently reported the three-dimensional structure, at 6.5 A reso...

journal_title：Journal of bacteriology

authors： Hirai T,Heymann JA,Maloney PC,Subramaniam S

更新日期：2003-03-01 00:00:00

abstract：:The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...

journal_title：Journal of bacteriology

authors： Anderson DG,Moseley SL

更新日期：1988-10-01 00:00:00

abstract：:Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 ...

journal_title：Journal of bacteriology

authors： Kloos DU,DiMarco AA,Elsemore DA,Timmis KN,Ornston LN

更新日期：1995-10-01 00:00:00

abstract：:Bacteriophage GA-1 infects Bacillus sp. strain G1R and has a linear double-stranded DNA genome with a terminal protein covalently linked to its 5' ends. GA-1 protein p6 is very abundant in infected cells and binds DNA with no sequence specificity. We show here that it binds in vivo to the whole viral genome, as detect...

journal_title：Journal of bacteriology

authors： Alcorlo M,Salas M,Hermoso JM

更新日期：2007-11-01 00:00:00

abstract：:Streptomyces azureus carried one copy or less of plasmid pSA1, which elicited pocks at 0.1 to 1.0%. Strain PK100 was isolated from the wild-type strain after UV irradiation. PK100 carried approximately 20 to 30 copies of pSA1.1, a derivative of pSA1. Plasmid pSA1.1 elicited pocks at 100% and inhibited spore and thiost...

journal_title：Journal of bacteriology

authors： Miyoshi YK,Ogata S,Hayashida S

更新日期：1986-10-01 00:00:00

abstract：:The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions ...

journal_title：Journal of bacteriology

authors： Huh WK,Kang SO

更新日期：1999-07-01 00:00:00

abstract：:An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be ...

journal_title：Journal of bacteriology

authors： Seeberg E,Steinum AL

更新日期：1980-03-01 00:00:00

abstract：:Clostridium perfringens is an anaerobic Gram-positive pathogen that causes many human and animal diseases, including food poisoning and gas gangrene. C. perfringens lacks flagella but possesses type IV pili (TFP). We have previously shown that C. perfringens can glide across an agar surface in long filaments composed ...

journal_title：Journal of bacteriology

authors： Liu H,Bouillaut L,Sonenshein AL,Melville SB

更新日期：2013-02-01 00:00:00

abstract：:The Bacillus spo0H gene codes for sigma H, which, as part of the RNA polymerase holoenzyme E sigma H, is responsible for the transcription of several genes which are expressed at the beginning of the sporulation process. In this communication, we examined the regulation of the spo0H gene of Bacillus subtilis by using ...

journal_title：Journal of bacteriology

authors： Weir J,Predich M,Dubnau E,Nair G,Smith I

更新日期：1991-01-01 00:00:00

abstract：:The foliar pathogen Pseudomonas syringae is a useful model for understanding the role of stress adaptation in leaf colonization. We investigated the mechanistic basis of differences in the osmotolerance of two P. syringae strains, B728a and DC3000. Consistent with its higher survival rates following inoculation onto l...

journal_title：Journal of bacteriology

authors： Freeman BC,Chen C,Yu X,Nielsen L,Peterson K,Beattie GA

更新日期：2013-10-01 00:00:00

abstract：:Restriction fragments containing the gene encoding cyanase, cynS, without its transcriptional regulatory sequences were placed downstream of lac and tac promoters in various pUC derivatives to maximize production of cyanase. Plasmid pSJ105, which contains the cynS gene and an upstream open reading frame, gave the high...

journal_title：Journal of bacteriology

authors： Sung YC,Anderson PM,Fuchs JA

更新日期：1987-11-01 00:00:00

abstract：:Diploid Saccharomyces cerevisiae cells induce YVH1 expression and enter the developmental pathway, leading to sporulation when starved for nitrogen. We show that yvh1 disruption causes a defect in spore maturation; overexpression of MCK1 or IME1 suppresses this yvh1 phenotype. While mck1 mutations are epistatic to tho...

journal_title：Journal of bacteriology

authors： Beeser AE,Cooper TG

更新日期：1999-09-01 00:00:00

abstract：:A mutant of Escherichia coli lacking hydroxyethylthiazole kinase (EC 2.7.1.50) was produced by a further mutation of a temperature-sensitive, auxotrophic mutant for hydroxyethylthiazole. The parent cells possessed two distinct enzymes capable of phosphorylating hydroxyethylthiazole: one was hydroxyethylthiazole kinase...

journal_title：Journal of bacteriology

authors： Mizote T,Nakayama H

更新日期：1989-06-01 00:00:00

abstract：:Proteins synthesized in Escherichia coli during recovery from starvation were resolved by two-dimensional polyacrylamide gel electrophoresis. Nine outgrowth-specific proteins, which appeared in two kinetic groups, that were not detected in either starved or exponential-phase cells were synthesized. Five other proteins...

journal_title：Journal of bacteriology

authors： Siegele DA,Guynn LJ

更新日期：1996-11-01 00:00:00

abstract：:In response to DNA damage or the inhibition of normal DNA replication in Escherichia coli, a set of some 20 unlinked operons is induced through the RecA-mediated cleavage of the LexA repressor. We examined the effect of this SOS response on the transposition of Tn5 and determined that the frequency of transposition is...

journal_title：Journal of bacteriology

authors： Weinreich MD,Makris JC,Reznikoff WS

更新日期：1991-11-01 00:00:00

abstract：:Horizontal gene transfer by conjugation plays a major role in bacterial evolution, allowing the acquisition of new traits, such as virulence and resistance to antibacterial agents. With the increased antibiotic resistance in bacterial pathogens, a better understanding of how bacteria modulate conjugation under changin...

journal_title：Journal of bacteriology

authors： Grace ED,Gopalkrishnan S,Girard ME,Blankschien MD,Ross W,Gourse RL,Herman C

更新日期：2015-03-01 00:00:00

abstract：:Nine natural isolates of Escherichia coli were examined, and the sequence of the entire 1,404 bases of the gnd gene (6-phosphogluconate dehydrogenase, EC 1.1.1.44) was determined. These isolates, along with E. coli K-12, constitute 10 strains for analysis. (The sequence of the E. coli K-12 gnd gene is known.) A total ...

journal_title：Journal of bacteriology

authors： Bisercić M,Feutrier JY,Reeves PR

更新日期：1991-06-01 00:00:00

abstract：:Cytochrome c' (Cyt c') is a c-type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in Rhodobacter sphaeroides 2.4.3, designated cycP, was isolated and sequenced. Northern blot analysis and beta-galactosidase assays demonstrated that cycP transcription increased as oxygen levels decreased an...

journal_title：Journal of bacteriology

authors： Choi PS,Grigoryants VM,Abruña HD,Scholes CP,Shapleigh JP

更新日期：2005-06-01 00:00:00

abstract：:Addition of casein hydrolysate to suspensions of washed, nonpigmented, nonproliferating Serratia marcescens incubating at 27 C induced biosynthesis of prodigiosin. Four amino acids of casein hydrolysate, dl-aspartic acid, l-glutamic acid, l-proline, and l-alanine caused formation of pigment when added individually. dl...

journal_title：Journal of bacteriology

authors： Williams RP,Gott CL,Qadri SM

更新日期：1971-05-01 00:00:00

abstract：:ShdA is a large outer membrane protein of the autotransporter family whose passenger domain binds the extracellular matrix proteins fibronectin and collagen I, possibly by mimicking the host ligand heparin. The ShdA passenger domain consists of approximately 1,500 amino acid residues that can be divided into two regio...

journal_title：Journal of bacteriology

authors： Kingsley RA,Abi Ghanem D,Puebla-Osorio N,Keestra AM,Berghman L,Bäumler AJ

更新日期：2004-08-01 00:00:00

abstract：:The bacterial flagellum is a biological nanomachine that rotates to allow bacteria to swim. For flagellar rotation, torque is generated by interactions between a rotor and a stator. The stator, which is composed of MotA and MotB subunit proteins in the membrane, is thought to bind to the peptidoglycan (PG) layer, whic...

journal_title：Journal of bacteriology

authors： Zhu S,Nishikino T,Takekawa N,Terashima H,Kojima S,Imada K,Homma M,Liu J

更新日期：2020-01-29 00:00:00

abstract：:The cir gene, which encodes the colicin I receptor protein and is regulated by both cellular iron content and growth temperature, was cloned into a multicopy-number plasmid. Physical mapping and complementation analysis established the position of cir between mgl and nfo on the Escherichia coli chromosome. A gene enco...

journal_title：Journal of bacteriology

authors： Griggs DW,Tharp BB,Konisky J

更新日期：1987-12-01 00:00:00

abstract：:We previously clarified that the chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 produces diacetylchitobiose (GlcNAc(2)) as an end product from chitin. Here we sought to identify enzymes in T. kodakaraensis that were involved in the further degradation of GlcNAc(2). Through a search of th...

journal_title：Journal of bacteriology

authors： Tanaka T,Fukui T,Atomi H,Imanaka T

更新日期：2003-09-01 00:00:00

abstract：:Bacillus subtilis OhrR is the prototype for the one-Cys family of organic peroxide-sensing regulatory proteins. Mutational analyses indicate that the high sensitivity of the active site cysteine (C15) to peroxidation requires three Tyr residues. Y29 and Y40 from the opposing subunit of the functional dimer hydrogen bo...

journal_title：Journal of bacteriology

authors： Soonsanga S,Fuangthong M,Helmann JD

更新日期：2007-10-01 00:00:00