Regulation and function of cytochrome c' in Rhodobacter sphaeroides 2.4.3.

Abstract:

:Cytochrome c' (Cyt c') is a c-type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in Rhodobacter sphaeroides 2.4.3, designated cycP, was isolated and sequenced. Northern blot analysis and beta-galactosidase assays demonstrated that cycP transcription increased as oxygen levels decreased and was not repressed under denitrifying conditions as observed in another Rhodobacter species. CO difference spectra performed with extracts of cells grown under different conditions revealed that Cyt c' levels were highest during photosynthetic denitrifying growth conditions. The increase in Cyt c' under this condition was higher than would be predicted from transcriptional studies. Electron paramagnetic resonance analysis of whole cells demonstrated that Cyt c' binds NO during denitrification. Mass spectrometric analysis of nitrogen oxides produced by cells and purified protein did not indicate that Cyt c' has NO reductase activity. Taken together, these results suggest a model where Cyt c' in R. sphaeroides 2.4.3 may shuttle NO to the membrane, where it can be reduced.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Choi PS,Grigoryants VM,Abruña HD,Scholes CP,Shapleigh JP

doi

10.1128/JB.187.12.4077-4085.2005

keywords:

subject

Has Abstract

pub_date

2005-06-01 00:00:00

pages

4077-85

issue

12

eissn

0021-9193

issn

1098-5530

pii

187/12/4077

journal_volume

187

pub_type

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