Integrative genetic element that reverses the usual target gene orientation.

abstract：:Synthesis of heme, measured by incorporation of iron-59, and of bacteriochlorophyll was studied with wild-type and mutant strains of Rhodopseudomonas spheroides. The wild type formed heme from glycine and succinate at one-fortieth the rate of bacteriochlorophyll under anaerobic-light conditions. Added delta-aminolevul...

journal_title：Journal of bacteriology

authors： Lascelles J,Hatch TP

更新日期：1969-05-01 00:00:00

abstract：:The gene for a new outer membrane-associated protease, designated OmpP, of Escherichia coli has been cloned and sequenced. The gene encodes a 315-residue precursor protein possessing a 23-residue signal sequence. Including conservative substitutions and omitting the signal peptides, OmpP is 87% identical to the outer ...

journal_title：Journal of bacteriology

authors： Kaufmann A,Stierhof YD,Henning U

更新日期：1994-01-01 00:00:00

abstract：:PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the v...

journal_title：Journal of bacteriology

authors： Trott AE,Stevens AM

更新日期：2001-01-01 00:00:00

abstract：:When the crude ribosomal fraction of Saccharomyces cerevisiae was separated into "light" and "heavy" fractions, fatty acid synthetase was concentrated in the former, whereas acetyl-Coenzyme A synthetase, fatty acid "desaturase," and squalene oxidocyclase were found in the latter. The "desaturase" sedimented with the r...

journal_title：Journal of bacteriology

authors： Klein HP,Volkmann CM,Leaffer MA

更新日期：1967-07-01 00:00:00

abstract：:Cyclic adenosine 3',5'-monophosphate isolated from Mycobacterium smegmatis cells was identified by thin-layer chromatography, stepwise conversion to adenosine 5'-monophosphate and adenosine, ultraviolet absorption spectrum, phosphate analysis, and detection by two relatively specific radioisotopic methods. ...

journal_title：Journal of bacteriology

authors： Lee CH

更新日期：1977-12-01 00:00:00

abstract：:The general stress response mediated by the sigma factor RpoS is important for survival of bacteria in adverse environments. A mutant unable to produce RpoS was constructed using the diazotrophic bacterium Azotobacter vinelandii strain UW. Under nondesiccating, solid-medium growth conditions the wild type was culturab...

journal_title：Journal of bacteriology

authors： Sandercock JR,Page WJ

更新日期：2008-02-01 00:00:00

abstract：:Stable, metabolically active protoplasts of Staphylococcus aureus have been prepared by the use of a staphylolytic enzyme produced by Aeromonas hydrophila. Respiratory and glycolytic rates and synthesis of nucleic acids, protein, and lipid in these protoplasts, stabilized variously in 1.1 M sucrose, 0.37 M sodium succ...

journal_title：Journal of bacteriology

authors： Coles NW,Gross R

更新日期：1973-09-01 00:00:00

abstract：:Burkholderia sp. strain TH2, a 2-chlorobenzoate (2CB)-degrading bacterium, metabolizes benzoate (BA) and 2CB via catechol. Two different gene clusters for the catechol ortho-cleavage pathway (cat1 and cat2) were cloned from TH2 and analyzed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot an...

journal_title：Journal of bacteriology

authors： Suzuki K,Ichimura A,Ogawa N,Hasebe A,Miyashita K

更新日期：2002-10-01 00:00:00

abstract：:The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC 1.3.99.1] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for...

journal_title：Journal of bacteriology

authors： Pollock JJ,Linder R,Salton MR

更新日期：1971-07-01 00:00:00

abstract：:Pili are functionally expressed during the predivisional and swarmer stages of the Caulobacter crescentus differentiation cycle. They appear on the developing swarmer pole and at the same cellular location as flagella and the phiCbK receptor sites. Pili disappear when the swarmer cell differentiates into a stalked cel...

journal_title：Journal of bacteriology

authors： Lagenaur C,Agabian N

更新日期：1977-07-01 00:00:00

abstract：:All Escherichia coli strains so far examined possess a chromosomally encoded nanATEK-yhcH operon for the catabolism of sialic acids. These unique nine-carbon sugars are synthesized primarily by higher eukaryotes and can be used as carbon, nitrogen, and energy sources by a variety of microbial pathogens or commensals. ...

journal_title：Journal of bacteriology

authors： Kalivoda KA,Steenbergen SM,Vimr ER,Plumbridge J

更新日期：2003-08-01 00:00:00

abstract：:As adhesion fimbriae are a major virulence factor for many pathogenic Gram-negative bacteria, they are also potential targets for antibodies. Fimbriae are commonly required for initiating the colonization that leads to disease, and their success as adhesion organelles lies in their ability to both initiate and sustain...

journal_title：Journal of bacteriology

authors： Singh B,Mortezaei N,Savarino SJ,Uhlin BE,Bullitt E,Andersson M

更新日期：2016-12-13 00:00:00

abstract：:Aerosols of Mycoplasma pneumoniae were studied at several relative humidities at a controlled temperature of 27 C. Production of an experimentally reproducible aerosol required preatomization of the organism in its suspending fluid and was dependent on the type of fluid used in atomization as well as on the procedures...

journal_title：Journal of bacteriology

authors： Wright DN,Bailey GD,Hatch MT

更新日期：1968-10-01 00:00:00

abstract：:During colonization of the alfalfa rhizosphere, Pseudomonas fluorescens F113 undergoes phenotypic variation, resulting in the appearance of colonies with different morphology. Among phenotypic variants, three isolates, C, F, and S were selected, with the C variant showing colony morphology identical to that of the ino...

journal_title：Journal of bacteriology

authors： Sánchez-Contreras M,Martín M,Villacieros M,O'Gara F,Bonilla I,Rivilla R

更新日期：2002-03-01 00:00:00

abstract：:Adaptation in bacterial chemotaxis involves reversible methylation of specific glutamate residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins. The specific sites of methylation in Salmonella enterica and Escherichia coli chemoreceptors, identified 2 decades ago, established a consensus seque...

journal_title：Journal of bacteriology

authors： Perez E,Zheng H,Stock AM

更新日期：2006-06-01 00:00:00

abstract：:Enzymes of the ATP-independent Deg serine endopeptidase family are very flexible with regard to their substrate specificity. Some family members cleave only one substrate, while others act as general proteases on unfolded substrates. The proteolytic activity of Deg proteases is regulated by PDZ protein interaction dom...

journal_title：Journal of bacteriology

authors： Huesgen PF,Scholz P,Adamska I

更新日期：2007-09-01 00:00:00

abstract：:The predicted amino acid sequence of Bacillus subtilis ycsK exhibits similarity to the GDSL family of lipolytic enzymes. Northern blot analysis showed that ycsK mRNA was first detected from 4 h after the onset of sporulation and that transcription of ycsK was dependent on SigK and GerE. The fluorescence of the YcsK-gr...

journal_title：Journal of bacteriology

authors： Masayama A,Kuwana R,Takamatsu H,Hemmi H,Yoshimura T,Watabe K,Moriyama R

更新日期：2007-03-01 00:00:00

abstract：:Photorhabdus is a genus of gram-negative Enterobacteriaceae that is pathogenic to insect larvae while also maintaining a mutualistic relationship with nematodes from the family Heterorhabditis, where the bacteria occupy the gut of the infective juvenile (IJ) stage of the nematode. In this study we describe the identif...

journal_title：Journal of bacteriology

authors： Bennett HP,Clarke DJ

更新日期：2005-01-01 00:00:00

abstract：:The metE gene of Salmonella typhimurium was cloned into plasmid pACYC184 by using a lambda gt7 -metE transducing phage as a source of metE DNA. The recombinant plasmid, designated pGS41 , carries a 12.8-kilobase-pair EcoRI insert fragment. The metE gene was subcloned from pGS41 into plasmid pBR322 on a 4.2-kilobase-pa...

journal_title：Journal of bacteriology

authors： Schulte LL,Stauffer LT,Stauffer GV

更新日期：1984-06-01 00:00:00

abstract：:Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation pro...

journal_title：Journal of bacteriology

authors： Canosi U,Siccardi AG,Falaschi A,Mazza G

更新日期：1976-04-01 00:00:00

abstract：:Gauntt, Charles J. (The University of Texas, Austin), and Royce Z. Lockart, Jr. Inhibition of Mengo virus by interferon. J. Bacteriol. 91:176-182. 1966.-The inhibition of Mengo virus replication in L cells resulting from interferon was studied quantitatively. Interferon was titrated on L cells with Western equine ence...

journal_title：Journal of bacteriology

authors： Gauntt CJ,Lockart RZ Jr

更新日期：1966-01-01 00:00:00

abstract：:We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requi...

journal_title：Journal of bacteriology

authors： Henriques AO,Bryan EM,Beall BW,Moran CP Jr

更新日期：1997-01-01 00:00:00

abstract：:Germination of dormant Bacillus subtilis spores with specific nutrient germinants is dependent on a number of inner membrane (IM) proteins, including (i) the GerA, GerB, and GerK germinant receptors (GRs) that respond to nutrient germinants; (ii) the GerD protein, essential for optimal GR function; and (iii) SpoVA pro...

journal_title：Journal of bacteriology

authors： Stewart KA,Setlow P

更新日期：2013-08-01 00:00:00

abstract：:The amounts of glycogen and trehalose have been measured in cells of a prototrophic diploid yeast strain subjected to a variety of nutrient limitations. Both glycogen and trehalose were accumulated in cells deprived specifically of nirogen, sulfur, or phosphorus, suggesting that reserve carbohydrate accumulation is a ...

journal_title：Journal of bacteriology

authors： Lillie SH,Pringle JR

更新日期：1980-09-01 00:00:00

abstract：:ToxR, the transmembrane regulatory protein required for expression of virulence factors in the human diarrheal pathogen Vibrio cholerae, directly activates and represses the transcription of two outer membrane porins, OmpU and OmpT, respectively. In an attempt to dissect the role of the OmpU and OmpT porins in viabili...

journal_title：Journal of bacteriology

authors： Provenzano D,Lauriano CM,Klose KE

更新日期：2001-06-01 00:00:00

abstract：:Molecular phylogenetic surveys have recently revealed an ecologically widespread crenarchaeal group that inhabits cold and temperate terrestrial and marine environments. To date these organisms have resisted isolation in pure culture, and so their phenotypic and genotypic characteristics remain largely unknown. To cha...

journal_title：Journal of bacteriology

authors： Schleper C,DeLong EF,Preston CM,Feldman RA,Wu KY,Swanson RV

更新日期：1998-10-01 00:00:00

abstract：:The content and distribution of autolysin were measured in temperature-sensitive morphological mutants of Bacillus subtilis. Strains RUB1000 and RUB1012 grew as rods at 30 C. At 45 C the mutants contained disproportionately less teichoic acid than peptidoglycan and grew as irregular spheres. The amount of enzyme that ...

journal_title：Journal of bacteriology

authors： Brown WC,Wilson CR,Lukehart S,Young FE,Shiflett MA

更新日期：1976-01-01 00:00:00

abstract：:Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

journal_title：Journal of bacteriology

authors： Atwell JL,Tennent JM,Lepper AW,Elleman TC

更新日期：1994-08-01 00:00:00

abstract：:Pizer, Lewis I. (University of Pennsylvania, Philadelphia). Glycine synthesis and metabolism in Escherichia coli. J. Bacteriol. 89:1145-1150. 1965.-A correlation was demonstrated between a nutritional requirement that can only be satisfied by glycine and the absence of the enzymatic capacity to interconvert l-serine a...

journal_title：Journal of bacteriology

authors： PIZER LI

更新日期：1965-04-01 00:00:00

abstract：:The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy on cellular metabolism and on the nitrogen catabolite repression of asparaginase II. Glutamate auxotrophic cells, incubated wi...

journal_title：Journal of bacteriology

authors： Kang L,Keeler ML,Dunlop PC,Roon RJ

更新日期：1982-07-01 00:00:00