Preparation of metabolically active Staphylococcus aureus protoplasts by use of the Aeromonas hydrophila lytic enzyme.

abstract：:The gene encoding OprF, a major outer membrane protein in Pseudomonas species (formerly known as type 1 pseudomonads), was thought to be constitutively transcribed from a single sigma 70 promoter immediately upstream of the gene. We now report the identification of a novel putative ECF (extracytoplasmic function) sigm...

journal_title：Journal of bacteriology

authors： Brinkman FS,Schoofs G,Hancock RE,De Mot R

更新日期：1999-08-01 00:00:00

abstract：:Bacillus megaterium mutants JV-9 and JV-10 are temperature sensitive for initiation of spore germination. At 46 C, they did not lose heat resistance, dipicolinic acid, or absorbance, indicating that the temperature-sensitive blocks are very early in the sequence of initiation reactions. Strain JV-9 was temperature sen...

journal_title：Journal of bacteriology

authors： Vary JC

更新日期：1975-01-01 00:00:00

abstract：:In contrast to previous findings, we demonstrate that the dissimilatory (bi)sulfite reductase genes (dsrAB) of Desulfobacula toluolica were vertically inherited. Furthermore, Desulfobacterium anilini and strain mXyS1 were identified, by dsrAB sequencing of 17 reference strains, as members of the donor lineage for thos...

journal_title：Journal of bacteriology

authors： Zverlov V,Klein M,Lücker S,Friedrich MW,Kellermann J,Stahl DA,Loy A,Wagner M

更新日期：2005-03-01 00:00:00

abstract：:In both prokaryotes and eukaryotes, hydroxyurea is suggested to inhibit DNA replication by inactivating ribonucleotide reductase and depleting deoxyribonucleoside triphosphate pools. In this study, we show that the inhibition of replication in Escherichia coli is transient even at concentrations of 0.1 M hydroxyurea a...

journal_title：Journal of bacteriology

authors： Nazaretyan SA,Savic N,Sadek M,Hackert BJ,Courcelle J,Courcelle CT

更新日期：2018-02-23 00:00:00

abstract：:Eubacterium sp. strain VPI 12708 is an anaerobic intestinal bacterium that has inducible bile acid 7-dehydroxylation activity. At least four new polypeptides were synthesized after addition of primary bile acids to the growth medium. One of these, of molecular weight 27,000 (P-27), was shown to be involved in the 7-de...

journal_title：Journal of bacteriology

authors： Coleman JP,White WB,Lijewski M,Hylemon PB

更新日期：1988-05-01 00:00:00

abstract：:A gcr2 null mutant of Saccharomyces cerevisiae grows well on glucose in spite of its lower level of glycolytic enzymes between triose phosphates and pyruvate. A quantitative analysis shows that these levels are adequate to the flux but glycerate phosphates are elevated. ...

journal_title：Journal of bacteriology

authors： Uemura H,Fraenkel DG

更新日期：1999-08-01 00:00:00

abstract：:The positions of the outer boundaries of the 5'- and 3'-conserved segment sequences of integrons found at several different locations have been determined. The position of the 5' end of the 5'-conserved segment is the same for six independently located integrons, In1 (R46), In2 (Tn21), In3 (R388), In4 (Tn1696), In5 (p...

journal_title：Journal of bacteriology

authors： Hall RM,Brown HJ,Brookes DE,Stokes HW

更新日期：1994-10-01 00:00:00

abstract：:The DNA transfer stage of conjugation requires the products of the F sex factor genes traMYDIZ and the cis-acting site oriT. Previous interpretation of genetic and protein analyses suggested that traD, traI, and traZ mapped as contiguous genes at the distal end of the transfer operon and saturated this portion of the ...

journal_title：Journal of bacteriology

authors： Traxler BA,Minkley EG Jr

更新日期：1987-07-01 00:00:00

abstract：:In the fungus Podospora anserina, the interaction between the nonallelic incompatible R and V genes has two consequences: a lytic reaction due to the synthesis of specific proteolytic enzymes, and a quenching in protein and ribonucleic acid synthesis. The incompatibility reaction when vegetative or sexual R and V cell...

journal_title：Journal of bacteriology

authors： Labarére J,Bègueret J,Bernet J

更新日期：1974-11-01 00:00:00

abstract：:Replication initiator proteins in bacteria not only allow DNA replication but also often regulate the rate of replication initiation as well. The regulation is mediated by limiting the synthesis or availability of initiator proteins. The applicability of this principle is demonstrated here for RctB, the replication in...

journal_title：Journal of bacteriology

authors： Pal D,Venkova-Canova T,Srivastava P,Chattoraj DK

更新日期：2005-11-01 00:00:00

abstract：:The extent of change in cytoplasmic proteins which accompanies yeast-to-mycelium morphogenesis of Candida albicans was analyzed by two-dimensional gel electrophoresis. Pure cultures of yeasts and true hyphae (i.e., without concomitant production of pseudohyphae) were grown in a synthetic low-sulfate medium. The two st...

journal_title：Journal of bacteriology

authors： Manning M,Mitchell TG

更新日期：1980-10-01 00:00:00

abstract：:The genome of Lactobacillus salivarius UCC118 includes a 242-kb megaplasmid, pMP118. We now show that 33 strains of L. salivarius isolated from humans and animals all harbor a megaplasmid, which hybridized with the repA and repE replication origin probes of pMP118. Linear megaplasmids that did not hybridize with the p...

journal_title：Journal of bacteriology

authors： Li Y,Canchaya C,Fang F,Raftis E,Ryan KA,van Pijkeren JP,van Sinderen D,O'Toole PW

更新日期：2007-09-01 00:00:00

abstract：:Cyclic di-AMP (c-di-AMP) is an essential and ubiquitous second messenger among bacteria. c-di-AMP regulates many cellular pathways through direct binding to several molecular targets in bacterial cells. c-di-AMP depletion is well known to destabilize the bacterial cell wall, resulting in increased bacteriolysis and en...

journal_title：Journal of bacteriology

authors： Massa SM,Sharma AD,Siletti C,Tu Z,Godfrey JJ,Gutheil WG,Huynh TN

更新日期：2020-11-19 00:00:00

abstract：:SecB is a cytosolic protein required for rapid and efficient export of particular periplasmic and outer membrane proteins in Escherichia coli. SecB promotes export by stabilizing newly synthesized precursor proteins in a nonnative conformation and by targeting the precursors to the inner membrane. Biochemical studies ...

journal_title：Journal of bacteriology

authors： Cook HA,Kumamoto CA

更新日期：1999-05-01 00:00:00

abstract：:Specific localization of the lysis (L) protein of bacteriophage MS2 in the cell wall of Escherichia coli was determined by immunoelectron microscopy. After induction of the cloned lysis gene, the cells were plasmolyzed, fixed, and embedded in either Epon or Lowicryl K4M. Polyclonal L-protein-specific antiserum was pur...

journal_title：Journal of bacteriology

authors： Walderich B,Höltje JV

更新日期：1989-06-01 00:00:00

abstract：:An aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 was purified 196-fold by a combination of Mono-Q, Reactive Green 19 agarose affinity, and hydroxyapatite chromatographies. The purified enzyme runs as a single band of 140 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular ...

journal_title：Journal of bacteriology

authors： Li T,Rosazza JP

更新日期：1997-06-01 00:00:00

abstract：:Formate dehydrogenase has traditionally been assumed to play an essential role in energy generation during growth on C(1) compounds. However, this assumption has not yet been experimentally tested in methylotrophic bacteria. In this study, a whole-genome analysis approach was used to identify three different formate d...

journal_title：Journal of bacteriology

authors： Chistoserdova L,Laukel M,Portais JC,Vorholt JA,Lidstrom ME

更新日期：2004-01-01 00:00:00

abstract：:The genetic organization of the foc gene cluster has been studied; six genes involved in the biogenesis of F1C fimbriae were identified. focA encodes the major fimbrial subunit, focC encodes a product that is indispensable for fimbria formation, focG and focH encode minor fimbrial subunits, and focI encodes a protein ...

journal_title：Journal of bacteriology

authors： Riegman N,Kusters R,Van Veggel H,Bergmans H,Van Bergen en Henegouwen P,Hacker J,Van Die I

更新日期：1990-02-01 00:00:00

abstract：:Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...

journal_title：Journal of bacteriology

authors： Graumann PL,Losick R

更新日期：2001-07-01 00:00:00

abstract：:Shapira, Jacob (Consolidated Veterans Administration Hospital, Little Rock, Ark.), Lois Lowden, and Ralph Hale. Inhibition of a thymine-deficient mutant of Escherichia coli by 5-substituted uracils. J. Bacteriol. 83:919-923. 1962.-Small inocula of well-washed cells of a thymine-requiring mutant of Escherichia coli wer...

journal_title：Journal of bacteriology

authors： SHAPIRA J,LOWDEN L,HALE R

更新日期：1962-04-01 00:00:00

abstract：:Rhodocyclales are representative of versatile bacteria that are able to utilize a wide variety of organic compounds for growth, but only a few strains have been isolated in pure culture thus far. Here we present the genome sequence of Methyloversatilis universalis FAM5(T), the first cultivable methylotrophic member of...

journal_title：Journal of bacteriology

authors： Kittichotirat W,Good NM,Hall R,Bringel F,Lajus A,Médigue C,Smalley NE,Beck D,Bumgarner R,Vuilleumier S,Kalyuzhnaya MG

更新日期：2011-09-01 00:00:00

abstract：:Escherichia coli MutY and MutS increase replication fidelity by removing adenines that were misincorporated opposite 7,8-dihydro-8-oxo-deoxyguanines (8-oxoG), G, or C. MutY DNA glycosylase removes adenines from these mismatches through a short-patch base excision repair pathway and thus prevents G:C-to-T:A and A:T-to-...

journal_title：Journal of bacteriology

authors： Bai H,Lu AL

更新日期：2007-02-01 00:00:00

abstract：:The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli. CsrA activity is antagonized by the untranslated RNA CsrB, to which it binds and forms a globula...

journal_title：Journal of bacteriology

authors： Suzuki K,Wang X,Weilbacher T,Pernestig AK,Melefors O,Georgellis D,Babitzke P,Romeo T

更新日期：2002-09-01 00:00:00

abstract：:SwmB is required for swimming motility in Synechococcus sp. strain WH8102. This highly repetitive 1.12-MDa polypeptide is associated with the cell surface, where it is arranged in a punctate manner. Inactivation of swmB does not affect the localization of SwmA, an S-layer protein also required for swimming. ...

journal_title：Journal of bacteriology

authors： McCarren J,Brahamsha B

更新日期：2007-02-01 00:00:00

abstract：:The morphological and physiological characteristics of 20 isolates of psychrophilic Bacillus were compared with 29 strains representing nine species of mesophilic Bacillus and 2 strains of Sporosarcina ureae to determine the taxonomic position of the psychrophiles. The psychrophiles formed four distinct groups which w...

journal_title：Journal of bacteriology

authors： Larkin JM,Stokes JL

更新日期：1967-10-01 00:00:00

abstract：:Nisin is a pore-forming antimicrobial peptide. The capacity of nisin to induce transmembrane movement of a fluorescent phospholipid in lipid vesicles was investigated. Unilamellar phospholipid vesicles that contained a fluorescent phospholipid (1-acyl-2-(6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)amino]caproyl)-sn-glycero-...

journal_title：Journal of bacteriology

authors： Moll GN,Konings WN,Driessen AJ

更新日期：1998-12-01 00:00:00

abstract：:A new insertion sequence, IS1634, has been identified in Mycoplasma mycoides subsp. mycoides small-colony type (SC). IS1634 shows structural and functional similarities to IS1549 of Mycobacterium smegmatis and with it seems to form a new class or family of insertion sequences. IS1634 has a size of 1,872 bp, including ...

journal_title：Journal of bacteriology

authors： Vilei EM,Nicolet J,Frey J

更新日期：1999-02-01 00:00:00

abstract：:Neisseria meningitidis serogroup B strain M986 was examined for the involvement of lipid intermediate(s) participating in the biosynthesis of the sialic acid capsular polysaccharide. The addition of exogenous undecaprenyl phosphate, phosphatidylethanolamine, or phosphatidylglycerol to particulate membranes, in the pre...

journal_title：Journal of bacteriology

authors： Masson L,Holbein BE

更新日期：1985-03-01 00:00:00

abstract：:A newly isolated bacterium, Pseudomonas geniculata N1, can efficiently degrade nicotine. Here we present a 4.51-Mb assembly of its genome, which is the first sequence of the P. geniculata group. The sequence contains the genes related to nicotine catabolism and may provide insights into its molecular mechanism for N-h...

journal_title：Journal of bacteriology

authors： Tang H,Yu H,Tai C,Huang K,Liu Y,Wang L,Yao Y,Wu G,Xu P

更新日期：2012-07-01 00:00:00

abstract：:High-resolution electron microscopy of polarly flagellated bacteria revealed that their flagella originate at a circular, differentiated portion of the cytoplasmic membrane approximately 25 nm in diameter. The flagella also have discs attaching them to the cell wall. These attachment discs are extremely resistant to l...

journal_title：Journal of bacteriology

authors： Vaituzis Z,Doetsch RN

更新日期：1969-10-01 00:00:00