Abstract:
:High-resolution electron microscopy of polarly flagellated bacteria revealed that their flagella originate at a circular, differentiated portion of the cytoplasmic membrane approximately 25 nm in diameter. The flagella also have discs attaching them to the cell wall. These attachment discs are extremely resistant to lytic damage and are firmly bound to the flagella. The cytoplasm beneath the flagellum contains a granulated basal body about 60 nm in diameter, and a specialized polar membrane. The existence of membrane-bound basal bodies is shown to be an artifact arising from adherence of cell wall and cytoplasmic membrane fragments to flagella in lysed preparations. Based on structures observed, a mechanism to explain bacterial flagellar movement is proposed. Flagella are considered to be anchored to the cell wall and activated by displacement of underlying cytoplasmic membrane to which they are also firmly attached. An explanation for the membrane displacement is given.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Vaituzis Z,Doetsch RNdoi
10.1128/JB.100.1.512-521.1969subject
Has Abstractpub_date
1969-10-01 00:00:00pages
512-21issue
1eissn
0021-9193issn
1098-5530journal_volume
100pub_type
杂志文章abstract::By using the isotope pairs (22)Na-(24)Na and (42)K-(86)Rb, the uptake and retention of Na and K was studied in the salt-tolerant Debaryomyces hansenii and in the less tolerant Saccharomyces cerevisiae at NaCl levels of 4 mm and 0.68, 1.35, and 2.7 m in the medium. The ratio of K to Na is much higher in the cells than ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.836-845.1969
更新日期:1969-11-01 00:00:00
abstract::Achromobacter xylosoxidans strain A8 was isolated from soil contaminated with polychlorinated biphenyls. It can use 2-chlorobenzoate and 2,5-dichlorobenzoate as sole sources of carbon and energy. This property makes it a good starting microorganism for further development toward a bioremediation tool. The genome of A....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01299-10
更新日期:2011-02-01 00:00:00
abstract::Exponentially growing populations of Bacillus subtilis contain two morphologically and functionally distinct cell types: motile individuals and nonmotile multicellular chains. Motility differentiation arises because RNA polymerase and the alternative sigma factor sigma(D) activate expression of flagellin in a subpopul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00521-09
更新日期:2009-09-01 00:00:00
abstract::The quorum-sensing system of Staphylococcus aureus, the accessory gene regulator (Agr) system, is responsible for increased transcription of certain exoprotein genes and decreased transcription of certain cell wall-associated proteins during the postexponential phase of growth. This regulation is important for virulen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.6.1793-1801.2004
更新日期:2004-03-01 00:00:00
abstract::The transport system for glycylglycine in Escherichia coli behaves like a shock-sensitive transport system. The initial rate of transport is reduced 85% by subjecting whole cells to osmotic shock, and glycylglycine is not transported by membrane vesicles. The energetics of transport was studied with strain ML 308-225 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.139-146.1974
更新日期:1974-10-01 00:00:00
abstract::Following arrest by UV-induced DNA damage, replication is restored through a sequence of steps that involve partial resection of the nascent DNA by RecJ and RecQ, branch migration and processing of the fork DNA surrounding the lesion by RecA and RecF-O-R, and resumption of DNA synthesis once the blocking lesion has be...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00290-12
更新日期:2012-08-01 00:00:00
abstract::The PhoQ/PhoP two-component system is repressed by divalent cations, such as Mg(2+) and Ca(2+), in the growth medium and stimulated by low pH and certain cationic antimicrobial peptides. In Escherichia coli, it was recently shown that the histidine kinase PhoQ is also modulated by at least two additional factors, the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06055-11
更新日期:2012-03-01 00:00:00
abstract::The wild strain of Serratia marcescens rapidly degraded threonine and formed aminoacetone in a medium containing glucose and urea. Extracts of this strain showed high threonine dehydrogenase and "biosynthetic" threonine deaminase activities, but no threonine aldolase activity. Threonine dehydrogenase-deficient strain ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.2.318-323.1978
更新日期:1978-08-01 00:00:00
abstract::Quorum sensing of Sinorhizobium meliloti relies on N-acyl-homoserine lactones (AHLs) as autoinducers. AHL production increases at high population density, and this depends on the AHL synthase SinI and two transcriptional regulators, SinR and ExpR. Our study demonstrates that ectopic expression of the gene rne, coding ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01471-13
更新日期:2014-04-01 00:00:00
abstract::Virgibacillus halodenitrificans 1806 is an endospore-forming halophilic bacterium isolated from salterns in Korea. Here, we report the draft genome sequence of V. halodenitrificans 1806, which may reveal the molecular basis of osmoadaptation and insights into carbon and anaerobic metabolism in moderate halophiles. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01280-12
更新日期:2012-11-01 00:00:00
abstract::Streptococcus faecalis obtains metabolic energy chiefly from the conversion of glucose to lactic acid; the present experiments deal with the mechanism of lactic acid translocation across the cytoplasmic membrane. Efflux of [(14)C]lactate from preloaded cells was accelerated by raising the external pH, and also by the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.3.1141-1148.1974
更新日期:1974-03-01 00:00:00
abstract::We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.16.4771-4778.2001
更新日期:2001-08-01 00:00:00
abstract::Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.719-727.1976
更新日期:1976-02-01 00:00:00
abstract::The T-POP transposon was employed in a general screen for tetracycline (Tet)-induced chromosomal loci that exhibited Tet-activated or Tet-repressed expression of a fliC-lac transcriptional fusion. Insertions that activated flagellar transcription were located in flagellar genes. T-POP insertions that exhibited Tet-dep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01177-08
更新日期:2009-03-01 00:00:00
abstract::A DNA sequence (brpA) which regulates the expression of the genes of the bialaphos biosynthesis pathway (bap) in Streptomyces hygroscopicus was identified and characterized. A newly isolated nonproducing mutant (NP57) had a pleiotropic defect involving at least 6 of the 13 known bap genes; only the step 6 conversion c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3482-3488.1987
更新日期:1987-08-01 00:00:00
abstract::We characterized three distinct families of repeated sequences in the genome of the cyanobacterium Calothrix sp. strain PCC 7601. These repeated sequences were present at a level of about 100 copies per Calothrix genome and consisted of tandemly amplified heptanucleotides. These elements were named short tandemly repe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.5.2755-2761.1990
更新日期:1990-05-01 00:00:00
abstract::A Pseudomonas strain produced an enzyme capable of deacylating 7 beta-(4-carboxybutanamido)cephalosporanic acid to 7-aminocephalosporanic acid in response to glutaric acid. The gene for the enzyme was cloned within the PstI site of pBR325 as a 7.35-kilobase-pair DNA segment from a mutant of this strain whose enzyme is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.1222-1228.1985
更新日期:1985-09-01 00:00:00
abstract::Chemotaxis to the aromatic acid 4-hydroxybenzoate (4-HBA) by Pseudomonas putida is mediated by PcaK, a membrane-bound protein that also functions as a 4-HBA transporter. PcaK belongs to the major facilitator superfamily (MFS) of transport proteins, none of which have so far been implicated in chemotaxis. Work with two...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.5068-5074.1999
更新日期:1999-08-01 00:00:00
abstract::The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.2.443-448.1975
更新日期:1975-08-01 00:00:00
abstract::The medium in which Rec(+) strains of Escherichia coli K-12 are grown affected their sensitivity to treatment with methyl methane sulfonate (MMS). Rec(+) cells grown to the stationary phase in glucose-enriched nutrient broth (GNB) were more resistant to MMS than cells grown in nutrient broth (NB). The repair of MMS-in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.1.267-272.1973
更新日期:1973-04-01 00:00:00
abstract::When titanium(III) citrate was used as electron donor for the reduction of methyl coenzyme M by the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum delta H, component A1 was no longer required. The simpler system thus obtained required components A2, A3, and C as well as catalytic amou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3946-3952.1988
更新日期:1988-09-01 00:00:00
abstract::Temperature-resistant pseudorevertants of the temperature-sensitive Escherichia coli mutant KNS19, harboring a mutation in rplX, the gene for ribosomal protein L24, were isolated, cloned, and sequenced. The codon GAC for the amino acid Asp in the temperature-sensitive mutant corresponding to position 84 in the protein...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4854-4856.1987
更新日期:1987-10-01 00:00:00
abstract::Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast extract-peptone-dextrose and minimal media, whereas class B auxotrophs grow on minimal medium and readily revert to grow on ye...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1545-1557.1975
更新日期:1975-12-01 00:00:00
abstract::Relatively high transfo mation frequencies were obtained with Micrococcus lysodeikticus cells, previou[ill]sly grown in defined broth, with a tris(hydroxymethyl)-aminomethane-glutamate b uffer containing Mg(++), Ca(++), Ba(++), or Sr(++). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1397-1399.1969
更新日期:1969-06-01 00:00:00
abstract::Five transposon Tn5 mutants of the procaryote Myxococcus xanthus had been shown previously to be defective in lipopolysaccharide biosynthesis (J. M. Fink,-M. Kalos, and J. F. Zissler, J. Bacteriol. 171:2033-2041, 1989). These mutants were studied for possible defects in gliding motility and multicellular development. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2042-2048.1989
更新日期:1989-04-01 00:00:00
abstract::Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.2.946-947.1977
更新日期:1977-05-01 00:00:00
abstract::A 4.2-kb plasmid (pLV22a) native to Bacteroides fragilis LV22 became fused to a transfer-deficient Bacteroides spp.-Escherichia coli shuttle vector by an inverse transposition event, resulting in a transferrable phenotype. The transfer phenotype was attributable to pLV22a, which was also capable of mobilization within...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4466-4473.1995
更新日期:1995-08-01 00:00:00
abstract::A strain of Neurospora crassa defective in amino acid transport can utilize a variety of amino acids for growth when readily metabolizable nitrogen is limiting. Growth is accompanied by the production of an extracellular deaminase that converts the amino acid to its respective keto acid plus equimolar quantities of ut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.583-589.1984
更新日期:1984-08-01 00:00:00
abstract::Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.19.6852-6855.2005
更新日期:2005-10-01 00:00:00
abstract::During batch growth of Alcaligenes eutrophus on benzoate-acetate mixtures, benzoate was the preferred substrate, with acetate consumption being delayed until the rate of benzoate consumption had diminished. This effect was attributed to a transcriptional control of the synthesis of acetyl coenzyme A (acetyl-CoA) synth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.5826-5833.1995
更新日期:1995-10-01 00:00:00