Transcriptional regulation of bialaphos biosynthesis in Streptomyces hygroscopicus.

Abstract:

:A DNA sequence (brpA) which regulates the expression of the genes of the bialaphos biosynthesis pathway (bap) in Streptomyces hygroscopicus was identified and characterized. A newly isolated nonproducing mutant (NP57) had a pleiotropic defect involving at least 6 of the 13 known bap genes; only the step 6 conversion could be detected. NP57 was more sensitive to bialaphos than its parent and had depressed levels of the demethylphosphinothricin acetyltransferase activity (step 10 in the pathway) which confers bialaphos resistance. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of extracts of this mutant showed that it lacked proteins corresponding to steps 5 and 10. NP57 lacked mRNAs for steps 5, 10, and 13. Bialaphos productivity of NP57 was restored by transformation with a plasmid containing a 5.9-kilobase DNA fragment which was adjacent to the structural gene cluster. Subcloning experiments showed that a 1.3-kilobase fragment from this primary clone restored all the defects of NP57. We conclude that brpA can activate the transcription of the bialaphos resistance gene as well as at least six other bap structural genes.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Anzai H,Murakami T,Imai S,Satoh A,Nagaoka K,Thompson CJ

doi

10.1128/jb.169.8.3482-3488.1987

subject

Has Abstract

pub_date

1987-08-01 00:00:00

pages

3482-8

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

杂志文章
  • Correlation between polyploidy and auxotrophic segregation in the imperfect yeast Candida albicans.

    abstract::In order to clarify the relationship between polyploidization and the capability of phenotypic switching in the imperfect yeast Candida albicans, two types of variants were isolated as segregants from a fusant, which produced a proportion of the cell population with a higher ploidy than the rest, either in a temperatu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.11.3345-3353.1994

    authors: Suzuki T,Hitomi A,Magee PT,Sakaguchi S

    更新日期:1994-06-01 00:00:00

  • Transcription regulation by tandem-bound FNR at Escherichia coli promoters.

    abstract::FNR is an Escherichia coli transcription factor that regulates the transcription of many genes in response to anaerobiosis. We have constructed a series of artificial FNR-dependent promoters, based on the melR promoter, in which a consensus FNR binding site was centered at position -41.5 relative to the transcription ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.20.5993-6004.2003

    authors: Barnard AM,Green J,Busby SJ

    更新日期:2003-10-01 00:00:00

  • Benzoate-dependent induction from the OP2 operator-promoter region of the TOL plasmid pWWO in the absence of known plasmid regulatory genes.

    abstract::Expression of the lower catabolic pathway of the TOL plasmid pWWO requires an aromatic acid inducer and the product of the xylS regulatory gene. Pseudomonas putida cells transformed with a plasmid containing the operator-promoter region of the lower pathway (OP2 [or Pm]), upstream from the catechol 2,3-dioxygenase str...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.8.3742-3746.1988

    authors: Cuskey SM,Sprenkle AB

    更新日期:1988-08-01 00:00:00

  • Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis.

    abstract::The ski22::Tn917lac insertion mutation in Bacillus subtilis was isolated in a screen for mutations that cause a defect in sporulation but are suppressed by the presence or overexpression of the histidine protein kinase encoded by kinA (spoIIJ). The ski22::Tn917lac insertion mutation was in ald, the gene encoding alani...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.21.6789-6796.1993

    authors: Siranosian KJ,Ireton K,Grossman AD

    更新日期:1993-11-01 00:00:00

  • Regulatory circuit for responses of nitrogen catabolic gene expression to the GLN3 and DAL80 proteins and nitrogen catabolite repression in Saccharomyces cerevisiae.

    abstract::We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. The expression of all these genes, with the exception of UGA1 and PUT2, also required a functional GLN3 protein. In addition, GLN3 protein was required for expression of the DAL1, DA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.1.64-73.1993

    authors: Daugherty JR,Rai R,el Berry HM,Cooper TG

    更新日期:1993-01-01 00:00:00

  • Modulation of the ComA-dependent quorum response in Bacillus subtilis by multiple Rap proteins and Phr peptides.

    abstract::In Bacillus subtilis, extracellular peptide signaling regulates several biological processes. Secreted Phr signaling peptides are imported into the cell and act intracellularly to antagonize the activity of regulators known as Rap proteins. B. subtilis encodes several Rap proteins and Phr peptides, and the processes r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00300-06

    authors: Auchtung JM,Lee CA,Grossman AD

    更新日期:2006-07-01 00:00:00

  • A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis.

    abstract::Lactococcus lactis, a gram-positive organism widely used in the food industry, is a potential candidate for the secretion of biologically useful proteins. We examined the secretion efficiency and capacity of L. lactis by using the Staphylococcus aureus nuclease (Nuc) as a heterologous model protein. When expressed in ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.7.1895-1903.1998

    authors: Le Loir Y,Gruss A,Ehrlich SD,Langella P

    更新日期:1998-04-01 00:00:00

  • Characterization of late acyltransferase genes of Yersinia pestis and their role in temperature-dependent lipid A variation.

    abstract::Yersinia pestis is an important human pathogen that is maintained in flea-rodent enzootic cycles in many parts of the world. During its life cycle, Y. pestis senses host-specific environmental cues such as temperature and regulates gene expression appropriately to adapt to the insect or mammalian host. For example, Y....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.4.1381-1388.2006

    authors: Rebeil R,Ernst RK,Jarrett CO,Adams KN,Miller SI,Hinnebusch BJ

    更新日期:2006-02-01 00:00:00

  • Stabilization of colicin E2 by bovine serum albumin.

    abstract::Colicin E2 was partially purified from Escherichia coli W3110. This preparation was remarkably stabilized by bovine serum albumin in a solution at neutral pH, as shown by dilution experiments and tests on heat stability of colicin. One killing unit of colicin E2 was estimated to correspond to one molecule of colicin E...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.1136-1137.1969

    authors: Mitsui E,Mizuno D

    更新日期:1969-11-01 00:00:00

  • Draft genome sequence of probiotic strain Lactobacillus rhamnosus R0011.

    abstract::Lactobacillus rhamnosus R0011 is a commercially available probiotic that is widely used in human dietary supplements and pharmaceutical products. We prepared a draft genome sequence consisting of 10 contigs totaling 2,900,620 bases and a G+C content of 46.7% for this strain. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06584-11

    authors: Tompkins TA,Barreau G,de Carvalho VG

    更新日期:2012-02-01 00:00:00

  • Variations in O-antigen biosynthesis and O-acetylation associated with altered phage sensitivity in Escherichia coli 4s.

    abstract::The O polysaccharide of the lipopolysaccharide (O antigen) of Gram-negative bacteria often serves as a receptor for bacteriophages that can make the phage dependent on a given O-antigen type, thus supporting the concept of the adaptive significance of the O-antigen variability in bacteria. The O-antigen layer also mod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02398-14

    authors: Knirel YA,Prokhorov NS,Shashkov AS,Ovchinnikova OG,Zdorovenko EL,Liu B,Kostryukova ES,Larin AK,Golomidova AK,Letarov AV

    更新日期:2015-03-01 00:00:00

  • Kinetic characterization of the monofunctional glycosyltransferase from Staphylococcus aureus.

    abstract::The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.7.2528-2532.2006

    authors: Terrak M,Nguyen-Distèche M

    更新日期:2006-04-01 00:00:00

  • Functional identification of the product of the Bacillus subtilis yvaL gene as a SecG homologue.

    abstract::Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.6.1786-1792.1999

    authors: van Wely KH,Swaving J,Broekhuizen CP,Rose M,Quax WJ,Driessen AJ

    更新日期:1999-03-01 00:00:00

  • Polymorphisms in inc proteins and differential expression of inc genes among Chlamydia trachomatis strains correlate with invasiveness and tropism of lymphogranuloma venereum isolates.

    abstract::Chlamydia trachomatis is a human bacterial pathogen that multiplies only within an intracellular membrane-bound vacuole, the inclusion. C. trachomatis includes ocular and urogenital strains, usually causing infections restricted to epithelial cells of the conjunctiva and genital mucosa, respectively, and lymphogranulo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01428-12

    authors: Almeida F,Borges V,Ferreira R,Borrego MJ,Gomes JP,Mota LJ

    更新日期:2012-12-01 00:00:00

  • Difference between manganese ion requirements of pediococci and enterococci.

    abstract::For maximal turbidity and dissimilatory activity, three strains of pediococci required the addition of Mn(2+) to Rogosa basal medium. Twenty-two strains of enterococci proved indifferent to this supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.627-628.1972

    authors: Efthymiou CJ,Joseph SW

    更新日期:1972-10-01 00:00:00

  • Characterization of genes for synthesis and catabolism of a new rhizopine induced in nodules by Rhizobium meliloti Rm220-3: extension of the rhizopine concept.

    abstract::Rhizopines are selective growth substrates synthesized in nodules only by strains of rhizobia capable of their catabolism. We report the isolation and study of genes for the synthesis and catabolism of a new rhizopine, scyllo-inosamine (sIa), from alfalfa nodules induced by Rhizobium meliloti Rm220-3. This compound is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.16.5205-5215.1993

    authors: Saint CP,Wexler M,Murphy PJ,Tempé J,Tate ME,Murphy PJ

    更新日期:1993-08-01 00:00:00

  • DE NOVO SYNTHESIS OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS.

    abstract::Welker, N. E. (Western Reserve University, Cleveland, Ohio and University of Illinois, Urbana), and L. Leon Campbell. De novo synthesis of alpha-amylase by Bacillus stearothermophilus. J. Bacteriol. 86:1202-1210. 1963.-The pH optimum for the synthesis of alpha-amylase by washed-cell suspensions was 6.7. alpha-Amylase ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.6.1202-1210.1963

    authors: WELKER NE,CAMPBELL LL

    更新日期:1963-12-01 00:00:00

  • Molecular cloning and functional expression of alternative oxidase from Candida albicans.

    abstract::The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.13.4098-4102.1999

    authors: Huh WK,Kang SO

    更新日期:1999-07-01 00:00:00

  • Analysis of Fur binding to operator sequences within the Neisseria gonorrhoeae fbpA promoter.

    abstract::The gene encoding Neisseria gonorrhoeae periplasmic binding protein FbpA contains two regions whose sequences exhibit homology with the Escherichia coli ferric uptake regulator protein (Fur) consensus binding sequence. In this study, DNase I footprinting experiments were employed to characterize the operator sequences...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.16.5020-5023.1996

    authors: Desai PJ,Angerer A,Genco CA

    更新日期:1996-08-01 00:00:00

  • Protein and ribonucleic acid syntheses in heat-damaged and heat-killed Escherichia coli.

    abstract::Protein and ribonucleic acid (RNA) syntheses were measured in both lethally injured and thermally damaged viable cells after heating at lethal temperatures. Immediately after heating, cells were incubated in growth media containing either [14C]leucine or [3H]uracil. The labeled cells were subsequently treated with pen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.2.492-498.1979

    authors: Dean RG,McGroarty EJ

    更新日期:1979-05-01 00:00:00

  • Suppression of a sensor kinase-dependent phenotype in Pseudomonas syringae by ribosomal proteins L35 and L20.

    abstract::The lemA gene of Pseudomonas syringae pv. syringae encodes the sensor kinase of a bacterial two-component signal transduction system. Phenotypes that are lemA dependent in P. syringae include lesion formation on bean and production of extracellular protease and the antibiotic syringomycin. Recently, the gacA gene has ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.6.1548-1555.1996

    authors: Kitten T,Willis DK

    更新日期:1996-03-01 00:00:00

  • Glycerol metabolism and PrfA activity in Listeria monocytogenes.

    abstract::Listeria monocytogenes is able to efficiently utilize glycerol as a carbon source. In a defined minimal medium, the growth rate (during balanced growth) in the presence of glycerol is similar to that in the presence of glucose or cellobiose. Comparative transcriptome analyses of L. monocytogenes showed high-level tran...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00259-08

    authors: Joseph B,Mertins S,Stoll R,Schär J,Umesha KR,Luo Q,Müller-Altrock S,Goebel W

    更新日期:2008-08-01 00:00:00

  • Hydroxyethylthiazole uptake in Escherichia coli: general properties and relationship between uptake and thiamine biosynthesis.

    abstract::Uptake of (35)S-hydroxyethylthiazole (4-methyl-5-hydroxyethylthiazole) by Escherichia coli intact cells was studied. Hydroxyethylthiazole was taken up in the presence and absence of glucose at the same rate. The uptake was almost proportional to a hydroxyethylthiazole concentration gradient up to 0.1 mM with no tenden...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.3.1280-1286.1973

    authors: Yamasaki H,Sanemori H,Yamada K,Kawasaki T

    更新日期:1973-12-01 00:00:00

  • Regulation of a common amidotransferase subunit.

    abstract::In Bacillus subtilis the trpX locus specifies a glutamine-binding protein designated subunit X, which forms a complex with subunit E to constitute the anthranilate synthase enzyme aggregate (EX) and subunit A to constitute the p-aminobenzoate synthase enzyme aggregate (AX). Subunit X confers upon these enzyme complexe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.419-425.1977

    authors: Kane JF

    更新日期:1977-11-01 00:00:00

  • Bacterial Cyclopropane Fatty Acid Synthase mRNA Is Targeted by Activating and Repressing Small RNAs.

    abstract::Altering membrane protein and lipid composition is an important strategy for maintaining membrane integrity during environmental stress. Many bacterial small RNAs (sRNAs) control membrane protein production, but sRNA-mediated regulation of membrane fatty acid composition is less well understood. The sRNA RydC was prev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00461-19

    authors: Bianco CM,Fröhlich KS,Vanderpool CK

    更新日期:2019-09-06 00:00:00

  • Isolation of Klebsiella aerogenes mutants cis-dominant for glutamine synthetase expression.

    abstract::We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These mutant strains were selected as glutamine-independent derivatives of a strain that is merodiploid for the glnA region and co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.671-679.1980

    authors: Rothstein DM,Magasanik B

    更新日期:1980-02-01 00:00:00

  • Adenylate energy charge in Saccharomyces cerevisiae during starvation.

    abstract::Bakers' yeast cells, Saccharomyces cerevisiae, if grown aerobically on ethanol or if grown aerobically on glucose and allowed to pass into stationary phase, with utilization of accumulated ethanol, maintain a normal value (0.8 to 0.9) of the adenylate energy charge during prolonged starvation. In contrast, cells grown...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.3.975-982.1975

    authors: Ball WJ Jr,Atkinson DE

    更新日期:1975-03-01 00:00:00

  • Factors regulating competence in transformation of streptococci.

    abstract::Pakula, Roman (University of Toronto, Toronto, Ontario, Canada). Factors regulating competence in transformation of streptococci. J. Bacteriol. 90:1320-1324. 1965.-The highly transformable group H Streptococcus strain Challis produced an exocellular competence-provoking enzyme capable of converting to the state of com...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.5.1320-1324.1965

    authors: Pakula R

    更新日期:1965-11-01 00:00:00

  • Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12.

    abstract::The bacterioferritin (BFR) of Escherichia coli K-12 is an iron-storage hemoprotein, previously identified as cytochrome b1. The bacterioferritin gene (bfr) has been cloned, sequenced, and located in the E. coli linkage map. Initially a gene fusion encoding a BFR-lambda hybrid protein (Mr 21,000) was detected by immuno...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3940-3947.1989

    authors: Andrews SC,Harrison PM,Guest JR

    更新日期:1989-07-01 00:00:00

  • A- and T-tract-mediated intrinsic curvature in native DNA between the binding site of the upstream activator NtrC and the nifLA promoter of Klebsiella pneumoniae facilitates transcription.

    abstract::The nif promoters of Klebsiella pneumoniae must be activated by proteins bound to upstream sequences which are thought to interact with the sigma54-RNA polymerase holoenzyme by DNA looping. NifA is the activator for most of the promoters, and integration host factor (IHF) mediates the DNA looping. While NtrC is the ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.17.5296-5302.1999

    authors: Cheema AK,Choudhury NR,Das HK

    更新日期:1999-09-01 00:00:00