Benzoate-dependent induction from the OP2 operator-promoter region of the TOL plasmid pWWO in the absence of known plasmid regulatory genes.

Abstract:

:Expression of the lower catabolic pathway of the TOL plasmid pWWO requires an aromatic acid inducer and the product of the xylS regulatory gene. Pseudomonas putida cells transformed with a plasmid containing the operator-promoter region of the lower pathway (OP2 [or Pm]), upstream from the catechol 2,3-dioxygenase structural gene, showed enzyme induction in the absence of known TOL plasmid regulatory genes. Induction was not seen in transformed Escherichia coli cells or in a P. putida mutant lacking chromosomally encoded benzoate catabolic functions.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Cuskey SM,Sprenkle AB

doi

10.1128/jb.170.8.3742-3746.1988

subject

Has Abstract

pub_date

1988-08-01 00:00:00

pages

3742-6

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

170

pub_type

杂志文章