Mutational analysis of flagellum-independent surface spreading of Serratia marcescens 274 on a low-agar medium.

Abstract:

:In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defective in both types of motility, were able to spread rapidly on the surface of low-agar (0.35%) media. We show here that some of the swarming-defective mutants are defective in the production of serrawettin W1, an extracellular cyclic lipopeptide produced by S. marcescens 274. When combined with a Fla defect, the serrawettin (Swt) mutants are deficient in spreading on low-agar media. The spreading deficiency can be overcome by serrawettin supplied extracellularly. Introduction of Fla defects into chemotaxis mutants does not affect this mode of surface translocation. These results suggest that spreading may be a passive form of translocation. We also report that swarming defects in all mutants showing a Dps phenotype (able to swarm within the inoculated area but unable to move outward) in the earlier study can be overcome by changing the commercial source of agar.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Matsuyama T,Bhasin A,Harshey RM

doi

10.1128/jb.177.4.987-991.1995

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

987-91

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

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