Active transport of D-alanine and related amino acids by whole cells of Bacillus subtilis.

Abstract:

:Whole cells of Bacillus subtilis transported d-alanine and l-alanine by two different systems. The high-affinity system (K(m) of 1 muM and V(max) of 0.6 to 0.8 nmol/min per mg of protein) was specific for the two stereoisomers of alanine. The low-affinity system (K(m) of 10 muM for l-alanine and 20 muM for d-alanine and glycine) had a V(max) of 5 to 12 nmol/min per mg of protein. This system transported glycine, d-cycloserine, and d-serine, in addition to d- and l-alanine. Azide inhibited the uptake of these amino acids and caused the efflux of d-alanine from preloaded cells. These data suggest that transport of these amino acids is energized by the electron transport chain.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Clark VL,Young FE

doi

10.1128/JB.120.3.1085-1092.1974

subject

Has Abstract

pub_date

1974-12-01 00:00:00

pages

1085-92

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

120

pub_type

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