Role of 3-Nitropropanoic acid in nitrate formation by Aspergillus flavus.

Abstract:

:Doxtader, K. G. (Cornell University, Ithaca, N.Y.), and M. Alexander. Role of 3-nitropropanoic acid in nitrate formation by Aspergillus flavus. J. Bacteriol. 91:1186-1191. 1966.-Aspergillus flavus formed nitrate, 3-nitropropanoic acid (3-NPA), kojic acid, and a substance tentatively identified as N-formyl-N-hydroxy-glycine during growth in a medium with ammonium as sole nitrogen source. The concentration of the nitro compound reached a maximum prior to the appearance of nitrate; the 3-NPA level subsequently decreased with a concomitant increase in nitrate concentration. Replacement cultures of A. flavus produced nitrate from culture filtrates containing 3-NPA or from synthetic 3-NPA but not when supplied with fresh ammonium-sucrose medium, the nitrate-nitrogen formed being equivalent to 50% of the quantity of the 3-NPA-nitrogen initially present. Neither nitrate nor 3-NPA was synthesized by the fungus during growth in media with low pH or low ammonium concentrations. It is proposed that 3-NPA is either an intermediate or is in equilibrium with an intermediate in nitrification by the fungus.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Doxtader KG,Alexander M

doi

10.1128/JB.91.3.1186-1191.1966

subject

Has Abstract

pub_date

1966-03-01 00:00:00

pages

1186-91

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

91

pub_type

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