pIIICTX, a predicted CTXphi minor coat protein, can expand the host range of coliphage fd to include Vibrio cholerae.

Abstract:

:CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Prior to the discovery that it was part of a prophage, orfU was initially described as an open reading frame of unknown function that lacked similarity to known protein sequences. Based on its size and position in the CTXphi genome, we hypothesized that OrfU may function in a manner similar to that of the coliphage fd protein pIII and mediate CTXphi infection as well as playing a role in CTXphi assembly and release. Deletion of orfU from CTXphi dramatically reduced the number of CTXphi virions detected in supernatants from CTXphi-bearing cells. This defect was complemented by expression of orfU in trans, thereby confirming a role for this gene in CTXphi assembly and/or release. To evaluate the requirement for OrfU in CTXphi infection, we introduced fragments of orfU into gIII in an fd derivative to create OrfU-pIII fusions. While fd is ordinarily unable to infect V. cholerae, an fd phage displaying the N-terminal 274 amino acids of OrfU could infect V. cholerae in a TCP- and TolA-dependent fashion. Since our findings indicate that OrfU functions as the CTXphi pIII, we propose to rename OrfU as pIII(CTX). Our data also provide new evidence for a conserved pathway for filamentous phage infection.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Heilpern AJ,Waldor MK

doi

10.1128/jb.185.3.1037-1044.2003

keywords:

subject

Has Abstract

pub_date

2003-02-01 00:00:00

pages

1037-44

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

185

pub_type

杂志文章
  • The Tn10-encoded tetracycline resistance mRNA contains a translational silencer in the 5' nontranslated region.

    abstract::We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance of that region for translation efficiency and mRNA stability. Transcriptional fusions of 17 mutants to lacZ expressed th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.8.2478-2484.1992

    authors: Flache P,Baumeister R,Hillen W

    更新日期:1992-04-01 00:00:00

  • An n-alkane-responsive promoter element found in the gene encoding the peroxisomal protein of Candida tropicalis does not contain a C(6) zinc cluster DNA-binding motif.

    abstract::When an asporogenic diploid yeast, Candida tropicalis, is cultivated on n-alkane, the expression of the genes encoding enzymes of the peroxisomal beta-oxidation pathway is highly induced. An upstream activation sequence (UAS) which can induce transcription in response to n-alkane (UAS(ALK)) was identified on the promo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.9.2492-2497.2000

    authors: Kanai T,Hara A,Kanayama N,Ueda M,Tanaka A

    更新日期:2000-05-01 00:00:00

  • Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis.

    abstract::Cells of the cyanobacterium Anabaena variabilis starved for phosphate for 3 days took up phosphate at about 100 times the rate of unstarved cells. Kinetic data suggested that a new transport system had been induced by starvation for phosphate. The inducible phosphate transport system was quickly repressed by addition ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.3.1143-1147.1988

    authors: Thiel T

    更新日期:1988-03-01 00:00:00

  • c-di-AMP Accumulation Impairs Muropeptide Synthesis in Listeria monocytogenes.

    abstract::Cyclic di-AMP (c-di-AMP) is an essential and ubiquitous second messenger among bacteria. c-di-AMP regulates many cellular pathways through direct binding to several molecular targets in bacterial cells. c-di-AMP depletion is well known to destabilize the bacterial cell wall, resulting in increased bacteriolysis and en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00307-20

    authors: Massa SM,Sharma AD,Siletti C,Tu Z,Godfrey JJ,Gutheil WG,Huynh TN

    更新日期:2020-11-19 00:00:00

  • In vitro construction of gshB::kan in Escherichia coli and use of gshB::kan in mapping the gshB locus.

    abstract::The Escherichia coli structural gene for glutathione synthetase, gshB, was cloned into pBR322. Plasmids containing gshB were able to complement the glutathione requirement of a trxA gshB double mutant, and cells containing the plasmids were found to have elevated levels of glutathione synthetase. A mutant gshB allele ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.5218-5221.1989

    authors: Daws T,Lim CJ,Fuchs JA

    更新日期:1989-09-01 00:00:00

  • Functional characterization of bacterial oligosaccharyltransferases involved in O-linked protein glycosylation.

    abstract::Protein glycosylation is an important posttranslational modification that occurs in all domains of life. Pilins, the structural components of type IV pili, are O glycosylated in Neisseria meningitidis, Neisseria gonorrhoeae, and some strains of Pseudomonas aeruginosa. In this work, we characterized the P. aeruginosa 1...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01318-07

    authors: Faridmoayer A,Fentabil MA,Mills DC,Klassen JS,Feldman MF

    更新日期:2007-11-01 00:00:00

  • Role of lipid intermediate(s) in the synthesis of serogroup B Neisseria meningitidis capsular polysaccharide.

    abstract::Neisseria meningitidis serogroup B strain M986 was examined for the involvement of lipid intermediate(s) participating in the biosynthesis of the sialic acid capsular polysaccharide. The addition of exogenous undecaprenyl phosphate, phosphatidylethanolamine, or phosphatidylglycerol to particulate membranes, in the pre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.3.861-867.1985

    authors: Masson L,Holbein BE

    更新日期:1985-03-01 00:00:00

  • Selection of fimbriate transductants of Salmonella typhimurium dependent on motility.

    abstract::The ability to form type 1 fimbriae (Fim(+)) was readily transduced to 159 out of 161 wild-type motile Fim(-) FIRN strains of Salmonella typhimurium with phage P22 propagated on a Fim(+) donor strain. Fim(+) clones were isolated from about 35% of tests after the fimbriate bacteria in the transduction mixture had been ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.3.655-658.1971

    authors: Old DC,Duguid JP

    更新日期:1971-09-01 00:00:00

  • Bacillus subtilis phenylalanyl-tRNA synthetase genes: cloning and expression in Escherichia coli and B. subtilis.

    abstract::The genes that encode the two subunits of Bacillus subtilis phenylalanyl-tRNA synthetase were cloned from alpha lambda library of chromosomal B. subtilis DNA by specific complementation of a thermosensitive Escherichia coli pheS mutation. Both genes (we named them pheS and pheT, analogous to the corresponding genes of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.1228-1232.1989

    authors: Brakhage AA,Putzer H,Shazand K,Röschenthaler RJ,Grunberg-Manago M

    更新日期:1989-02-01 00:00:00

  • Two different rpf clusters distributed among a population of Stenotrophomonas maltophilia clinical strains display differential diffusible signal factor production and virulence regulation.

    abstract::The quorum-sensing (QS) system present in the emerging nosocomial pathogen Stenotrophomonas maltophilia is based on the signaling molecule diffusible signal factor (DSF). Production and detection of DSF are governed by the rpf cluster, which encodes the synthase RpfF and the sensor RpfC, among other components. Despit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01540-14

    authors: Huedo P,Yero D,Martínez-Servat S,Estibariz I,Planell R,Martínez P,Ruyra A,Roher N,Roca I,Vila J,Daura X,Gibert I

    更新日期:2014-07-01 00:00:00

  • AmiC functions as an N-acetylmuramyl-l-alanine amidase necessary for cell separation and can promote autolysis in Neisseria gonorrhoeae.

    abstract::Neisseria gonorrhoeae is prone to undergo autolysis under many conditions not conducive to growth. The role of autolysis during gonococcal infection is not known, but possible advantages for the bacterial population include provision of nutrients to a starving population, modulation of the host immune response by rele...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00724-06

    authors: Garcia DL,Dillard JP

    更新日期:2006-10-01 00:00:00

  • Purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in Rhodococcus ruber.

    abstract::The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.14.4328-4337.1994

    authors: Pieper-Fürst U,Madkour MH,Mayer F,Steinbüchel A

    更新日期:1994-07-01 00:00:00

  • Temporal global changes in gene expression during temperature transition in Yersinia pestis.

    abstract::DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37 degrees C in the presence and absence of Ca2+. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.18.6298-6305.2004

    authors: Motin VL,Georgescu AM,Fitch JP,Gu PP,Nelson DO,Mabery SL,Garnham JB,Sokhansanj BA,Ott LL,Coleman MA,Elliott JM,Kegelmeyer LM,Wyrobek AJ,Slezak TR,Brubaker RR,Garcia E

    更新日期:2004-09-01 00:00:00

  • Protein phosphorylation in Bradyrhizobium japonicum bacteroids and cultures.

    abstract::Protein phosphorylation was demonstrated in Bradyrhizobium japonicum bacteroids in vivo and in cultures in vivo and in vitro. Comparison of in vivo-labeled phosphoproteins of bacteroids and of cultured cells showed differences in both the pattern and intensity of labeling. In cultured cells, comparison of the labeling...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.6.3420-3426.1989

    authors: Karr DB,Emerich DW

    更新日期:1989-06-01 00:00:00

  • Roles of a ribosome-binding site and mRNA secondary structure in differential expression of Shiga toxin genes.

    abstract::The Shiga toxin operon (stx) is composed of two genes for the A and B subunits, which are transcribed from a promoter 5' to the stxA gene. The 1A:5B subunit stoichiometry of the holotoxin suggests that the stxA and stxB genes are differentially regulated. In a previous study, we demonstrated the existence of a second ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.3.597-603.1993

    authors: Habib NF,Jackson MP

    更新日期:1993-02-01 00:00:00

  • Cell division in a species of Erwinia IV. Metabolic blocks in panothenate biosynthesis and their relationship to inhibition of cell division.

    abstract::Grula, Mary M. (Oklahoma State University, Stillwater) and E. A. Grula. Cell division in a species of Erwinia. IV. Metabolic blocks in pantothenate biosynthesis and their relationship to inhibition of cell division. J. Bacteriol. 83:989-997. 1962.-Four compounds that inhibit cell division in an Erwinia sp., d-serine, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.5.989-997.1962

    authors: GRULA MM,GRULA EA

    更新日期:1962-05-01 00:00:00

  • Effect of glucose starvation on the nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase of yeast.

    abstract::Yeast cells growing on mineral medium plus ammonia and glucose contained high levels of nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase activity, as measured in crude extracts. After suspension of cells in fresh medium lacking glucose, there was a loss of the glutamate dehydrogenase activ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.780-785.1978

    authors: Mazón MJ

    更新日期:1978-02-01 00:00:00

  • Relationship between cell wall, cytoplasmic membrane, and bacterial motility.

    abstract::High-resolution electron microscopy of polarly flagellated bacteria revealed that their flagella originate at a circular, differentiated portion of the cytoplasmic membrane approximately 25 nm in diameter. The flagella also have discs attaching them to the cell wall. These attachment discs are extremely resistant to l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.512-521.1969

    authors: Vaituzis Z,Doetsch RN

    更新日期:1969-10-01 00:00:00

  • Complementation of the essential peptidoglycan transpeptidase function of penicillin-binding protein 2 (PBP2) by the drug resistance protein PBP2A in Staphylococcus aureus.

    abstract::The essential function of penicillin-binding protein 2 (PBP2) in methicillin-susceptible Staphylococcus aureus RN4220 was clearly established by placing the pbp2 gene under control of the inducible P(spac) promoter; the resulting bacteria were unable to grow in the absence of inducer. In contrast, the deficit in PBP2 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.22.6525-6531.2001

    authors: Pinho MG,Filipe SR,de Lencastre H,Tomasz A

    更新日期:2001-11-01 00:00:00

  • Biosynthesis of phospholipids in Bacillus megaterium.

    abstract::Information on the biosynthesis of phospholipids in bacteria has been derived principally from the study of Escherichia coli and other gram-negative organisms. We have now carried out a detailed study of the pathways of phospholipid biosynthesis in the gram-positive organism Bacillus megarterium KM in relation to inve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.996-1007.1979

    authors: Langley KE,Yaffe MP,Kennedy EP

    更新日期:1979-12-01 00:00:00

  • Activation of protease-constitutive recA proteins of Escherichia coli by rRNA and tRNA.

    abstract::The RecA proteins of the unusually strong protease-constitutive mutants recA1202 and recA1211 can use RNA in addition to single-stranded DNA (ssDNA) as a cofactor in the cleavage of the LexA repressor in vitro. In the presence of rRNA or tRNA, the effectiveness of these proteins decreased in the order RecA1202 greater...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4823-4827.1988

    authors: Wang WB,Tessman ES,Tessman I

    更新日期:1988-10-01 00:00:00

  • Plasmid formation after lambda bacteriophage infection of Escherichia coli-Salmonella typhosa hybrids.

    abstract::Defective phage lambdadg, when present in certain Salmonella typhosa hybrids, could be eliminated with acridine orange or ethidium bromide treatment. The lambdadg deoxyribonucleic acid could be separated from the S. typhosa host deoxyribonucleic acid as a distinctly covalently closed molecule. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.1.288-290.1970

    authors: Falkow S,Baron LS

    更新日期:1970-04-01 00:00:00

  • Persistent cyclic herpes simplex virus infection in vitro. IV. Changes in the severity of the infections in the presence of antibody.

    abstract::Hampar, Berge (National Institute of Dental Research, Bethesda, Md.). Persistent cyclic herpes simplex virus infection in vitro. IV. Changes in the severity of the infections in the presence of antibody. J. Bacteriol. 92:1741-1747. 1966.-The severity of persistent herpes simplex virus (HSV) infections maintained in th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.6.1741-1747.1966

    authors: Hampar B

    更新日期:1966-12-01 00:00:00

  • Analysis of hemF gene function and expression in Rhodobacter sphaeroides 2.4.1.

    abstract::The hemF gene of Rhodobacter sphaeroides 2.4.1 is predicted to code for an oxygen-dependent coproporphyrinogen III oxidase. We found that a HemF- mutant strain is unable to grow under aerobic conditions. We also determined that hemF expression is controlled by oxygen, which is mediated, at least in part, by the respon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.2.801-804.2006

    authors: Zeilstra-Ryalls JH,Schornberg KL

    更新日期:2006-01-01 00:00:00

  • T-POP array identifies EcnR and PefI-SrgD as novel regulators of flagellar gene expression.

    abstract::The T-POP transposon was employed in a general screen for tetracycline (Tet)-induced chromosomal loci that exhibited Tet-activated or Tet-repressed expression of a fliC-lac transcriptional fusion. Insertions that activated flagellar transcription were located in flagellar genes. T-POP insertions that exhibited Tet-dep...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01177-08

    authors: Wozniak CE,Lee C,Hughes KT

    更新日期:2009-03-01 00:00:00

  • Aspartokinase III, a new isozyme in Bacillus subtilis 168.

    abstract::A previously undetected Bacillus subtilis aspartokinase isozyme, which we have called aspartokinase III, has been characterized. The new isozyme was most readily detected in extracts of cells grown with lysine, which repressed aspartokinase II and induced aspartokinase III, or in extracts of strain VS11, a mutant lack...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.1.218-223.1990

    authors: Graves LM,Switzer RL

    更新日期:1990-01-01 00:00:00

  • Synergistic effect of himA and gyrB mutations: evidence that him functions control expression of ilv and xyl genes.

    abstract::We have constructed Escherichia coli strains containing mutations at two different loci, both originally selected for failure to support lambda site-specific recombination: himA and gyrB-him(Ts). Although the gyrB-him(Ts) mutations by themselves reduce supercoiling at high temperature, the double mutants show a far gr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.2.484-489.1984

    authors: Friedman DI,Olson EJ,Carver D,Gellert M

    更新日期:1984-02-01 00:00:00

  • Klebsiella serotype 25 capsular polysaccharide: primary structure and depolymerization by a bacteriophage-borne glycanase.

    abstract::By partial acid hydrolysis, methylation and gas-liquid chromatography-mass spectrometry of the methylated monomers (as the alditol acetates), mass spectrometry of trimethylsilylated disaccharide alditols, as well as proton magnetic resonance, the primary structure of the Klebsiella serotype 25 capsular polysaccharide ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.366-374.1977

    authors: Niemann H,Kwiatkowski B,Westphal U,Stirm S

    更新日期:1977-04-01 00:00:00

  • Messenger ribonucleic acid of dormant spores of Bacillus subtilis.

    abstract::Evidence of the presence of messenger ribonucleic acid (mRNA) in dormant spores of Bacillus subtilis has been obtained. The bulk RNA from spores was isolated and labeled in vitro with tritiated dimethyl sulfate. The spore RNA hybridized to 2.4 to 3.2% of the B. subtilis genome. The RNA hybridized to both the complemen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.2.514-521.1974

    authors: Jeng YH,Doi RH

    更新日期:1974-08-01 00:00:00

  • Antibodies against Staphylococcus aureus in nonimmunized rabbits.

    abstract::Cohen, Jay O. (Communicable Disease Center, Atlanta, Ga.), Glenda S. Cowart, and William B. Cherry. Antibodies against Staphylococcus aureus in nonimmunized rabbits. J. Bacteriol. 82:110-114. 1961.-Antibody against staphylococci was demonstrated in the serum of each of 36 nonimmunized specific-pathogen-free rabbits th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.1.110-114.1961

    authors: COHEN JO,COWART GS,CHERRY WB

    更新日期:1961-07-01 00:00:00