Purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in Rhodococcus ruber.

Abstract:

:The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 which was recently localized downstream of the PHA synthase gene (U. Pieper and A. Steinbüchel, FEMS Microbiol. Lett. 96:73-80, 1992). A recombinant strain of Escherichia coli XL1-Blue carrying the hybrid plasmid (pSKXA10*) with open reading frame 3 overexpressed the GA14 protein. The GA14 protein was subsequently purified in a three-step procedure including chromatography on DEAE-Sephacel, phenyl-Sepharose CL-4B, and Superose 12. Determination of the molecular weight by gel filtration as well as electron microscopic studies indicates that a tetrameric structure of the recombinant, native GA14 protein is most likely. Immunoelectron microscopy demonstrated a localization of the GA14 protein at the periphery of PHA granules as well as close to the cell membrane in R. ruber. Investigations of PHA-leaky and PHA-negative mutants of R. ruber indicated that expression of the GA14 protein depended strongly on PHA synthesis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Pieper-Fürst U,Madkour MH,Mayer F,Steinbüchel A

doi

10.1128/jb.176.14.4328-4337.1994

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

4328-37

issue

14

eissn

0021-9193

issn

1098-5530

journal_volume

176

pub_type

杂志文章
  • A-factor and phosphate depletion signals are transmitted to the grixazone biosynthesis genes via the pathway-specific transcriptional activator GriR.

    abstract::Grixazone (GX), which is a diffusible yellow pigment containing a phenoxazinone chromophore, is one of the secondary metabolites under the control of A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) in Streptomyces griseus. GX production is also induced by phosphate starvation. The whole biosynthesis gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00055-07

    authors: Higashi T,Iwasaki Y,Ohnishi Y,Horinouchi S

    更新日期:2007-05-01 00:00:00

  • The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology.

    abstract::Electron cryotomography was used to analyze the structure of the Lyme disease spirochete, Borrelia burgdorferi. This methodology offers a new means for studying the native architecture of bacteria by eliminating the chemical fixing, dehydration, and staining steps of conventional electron microscopy. Using electron cr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01288-08

    authors: Charon NW,Goldstein SF,Marko M,Hsieh C,Gebhardt LL,Motaleb MA,Wolgemuth CW,Limberger RJ,Rowe N

    更新日期:2009-01-01 00:00:00

  • IDENTITY OF THE PINK-PIGMENTED METHANOL-OXIDIZING BACTERIA AS VIBRIO EXTORQUENS.

    abstract::Stocks, Peter K. (Louisiana State University, Baton Rouge), and C. S. McCleskey. Identity of the pink-pigmented methanol-oxidizing bacteria as Vibrio extorquens. J. Bacteriol. 88:1065-1070. 1964.-Pink-pigmented bacteria isolated from enrichment cultures of methane oxidizers were found to possess similar morphological,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.4.1065-1070.1964

    authors: STOCKS PK,MCCLESKEY CS

    更新日期:1964-10-01 00:00:00

  • PREFERENTIAL SYNTHESIS OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS IN THE PRESENCE OF 5-METHYL-TRYPTOPHAN.

    abstract::Welker, N. E. (Western Reserve University, Cleveland, Ohio), and L. Leon Campbell. Preferential synthesis of alpha-amylase by Bacillus stearothermophilus in the presence of 5-methyl-tryptophan. J. Bacteriol. 87:828-831. 1964.-Washed-cell suspensions of Bacillus stearothermophilus induced with pure maltose preferential...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.4.828-831.1964

    authors: WELKER NE,CAMPBELL LL

    更新日期:1964-04-01 00:00:00

  • ACTION OF A HISTIDINE ANALOGUE, 1,2,4-TRIAZOLE-3-ALANINE, IN SALMONELLA TYPHIMURIUM.

    abstract::Levin, Alfred P. (The Johns Hopkins University, Baltimore, Md.), and Philip E. Hartman. Action of a histidine analogue, 1,2,4-triazole-3-alanine, in Salmonella typhimurium. J. Bacteriol. 86:820-828. 1963.-The effect of the histidine analogue, 1,2,4-triazole-3-alanine (TRA), on growth and enzyme synthesis in histidine ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.4.820-828.1963

    authors: LEVIN AP,HARTMAN PE

    更新日期:1963-10-01 00:00:00

  • Influences of various amino acids on tryptophan-mediated control of the tryptophan biosynthetic enzymes in Escherichia coli.

    abstract::Lysates of Escherichia coli Ymel obtained from cultures grown in the absence of tryptophan in minimal medium supplemented with 0.1% casein hydrolysate show an approximate fivefold increase in steady-state specific activity of both anthranilate synthetase and tryptophan synthetase A protein relative to cultures grown i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.1181-1191.1971

    authors: Stubbs JD,Stubbs EA

    更新日期:1971-12-01 00:00:00

  • Cj1386 is an ankyrin-containing protein involved in heme trafficking to catalase in Campylobacter jejuni.

    abstract::Campylobacter jejuni, a microaerophilic bacterium, is the most frequent cause of human bacterial gastroenteritis. C. jejuni is exposed to harmful reactive oxygen species (ROS) produced during its own normal metabolic processes and during infection from the host immune system and from host intestinal microbiota. These ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05740-11

    authors: Flint A,Sun YQ,Stintzi A

    更新日期:2012-01-01 00:00:00

  • Mechanisms of biodegradation of metal-citrate complexes by Pseudomonas fluorescens.

    abstract::Biodegradation of metal-citrate complexes by Pseudomonas fluorescens depends on the nature of the complex formed between the metal and citric acid. Bidentate Fe(III)-, Ni-, and Zn-citrate complexes were readily biodegraded, but the tridentate Cd- and Cu-citrate, and U-citrate complexes were not. The biodegradation of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.8.1989-1993.1995

    authors: Joshi-Tope G,Francis AJ

    更新日期:1995-04-01 00:00:00

  • Repair of x-ray-induced deoxyribonucleic acid single-strand breaks in xth mutants of Escherichia coli.

    abstract::An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1424-1427.1980

    authors: Seeberg E,Steinum AL

    更新日期:1980-03-01 00:00:00

  • Proteolytic Cleavage of the Immunodominant Outer Membrane Protein rOmpA in Rickettsia rickettsii.

    abstract::Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as members of a family of autotransporter proteins. Previously, it was demonstrated tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00826-16

    authors: Noriea NF,Clark TR,Mead D,Hackstadt T

    更新日期:2017-02-28 00:00:00

  • Conservation of plasmid maintenance functions between linear and circular plasmids in Borrelia burgdorferi.

    abstract::The Lyme disease agent Borrelia burgdorferi maintains both linear and circular plasmids that appear to be essential for mammalian infection. Recent studies have characterized the circular plasmid regions that confer autonomous replication, but the genetic elements necessary for linear plasmid maintenance have not been...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.10.3202-3209.2003

    authors: Stewart PE,Chaconas G,Rosa P

    更新日期:2003-05-01 00:00:00

  • Overexpression of the response regulator evgA of the two-component signal transduction system modulates multidrug resistance conferred by multidrug resistance transporters.

    abstract::Overexpression of evgA, a response regulator of a two-component system, increased multidrug efflux in Escherichia coli. Since overexpression of the emrKY operon, which is controlled by evgAS, could account only for deoxycholate resistance, the evgAS locus apparently controls expression of at least one other multidrug ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.4.1455-1458.2001

    authors: Nishino K,Yamaguchi A

    更新日期:2001-02-01 00:00:00

  • Isolation of Neurospora crassa bradytrophs.

    abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.1133-1136.1979

    authors: Kinsey JA

    更新日期:1979-12-01 00:00:00

  • YshB Promotes Intracellular Replication and Is Required for Salmonella Virulence.

    abstract::Salmonella virulence requires the initial invasion of host cells, followed by modulation of the intracellular environment for survival and replication. In an effort to characterize the role of small RNAs in Salmonella pathogenesis, we inadvertently identified a 5-kDa protein named YshB that is involved in the intracel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00314-19

    authors: Bomjan R,Zhang M,Zhou D

    更新日期:2019-08-08 00:00:00

  • Effect of perturbation of ATP level on the activity and regulation of nitrogenase in Rhodospirillum rubrum.

    abstract::Nitrogenase activity in Rhodospirillum rubrum and in some other photosynthetic bacteria is regulated in part by the availability of light. This regulation is through a posttranslational modification system that is itself regulated by P(II) homologs in the cell. P(II) is one of the most broadly distributed regulatory p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00585-09

    authors: Zhang Y,Pohlmann EL,Roberts GP

    更新日期:2009-09-01 00:00:00

  • Regulation of cell division in Escherichia coli: SOS induction and cellular location of the sulA protein, a key to lon-associated filamentation and death.

    abstract::Mutations in sulA (sfiA) block the filamentation and death of capR (lon) mutants that occur after treatments that either damage DNA or inhibit DNA replication and thereby induce the SOS response. Previous sulA-lacZ gene fusion studies showed that sulA is transcriptionally regulated by the SOS response system (lexA/rec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.2.551-561.1984

    authors: Schoemaker JM,Gayda RC,Markovitz A

    更新日期:1984-05-01 00:00:00

  • Expression and characterization of Campylobacter jejuni benzoylglycine amidohydrolase (Hippuricase) gene in Escherichia coli.

    abstract::The basis for the difference between Campylobacter jejuni and Campylobacter coli is the presence and expression of the N-benzoylglycine amidohydrolase (hippuricase) gene only in C. jejuni. A pBR322 recombinant clone (pHIP-O) of C. jejuni TGH9011 capable of converting hippuric acid into benzoic acid and glycine, the ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.9.2396-2402.1995

    authors: Hani EK,Chan VL

    更新日期:1995-05-01 00:00:00

  • Electron microscopy of Staphylococcus aureus cells and cell walls after treatment with lysozyme Chalaropsis.

    abstract::Lysozyme Chalaropsis, a beta-1,4-N,6-O-diacetylmuramidase, attacks the cell wall murein of Staphylococcus aureus H in a random manner. In hypertonic sucrose solutions, true protoplasts are produced and, in the process, mesosomal tubules are extruded into the medium. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.2.900-903.1974

    authors: Robinson JP,Robinson RD,Hash JH

    更新日期:1974-02-01 00:00:00

  • Clostridium perfringens spore germination: characterization of germinants and their receptors.

    abstract::Clostridium perfringens food poisoning is caused by type A isolates carrying a chromosomal enterotoxin (cpe) gene (C-cpe), while C. perfringens-associated non-food-borne gastrointestinal (GI) diseases are caused by isolates carrying a plasmid-borne cpe gene (P-cpe). C. perfringens spores are thought to be the importan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01748-07

    authors: Paredes-Sabja D,Torres JA,Setlow P,Sarker MR

    更新日期:2008-02-01 00:00:00

  • CHARACTERISTICS OF CLOSTRIDIUM PERFRINGENS STRAINS ASSOCIATED WITH FOOD AND FOOD-BORNE DISEASE.

    abstract::Hall, Herbert E. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Robert Angelotti, Keith H. Lewis, and Milton J. Foter. Characteristics of Clostridium perfringens strains associated with food and food-borne disease. J. Bacteriol. 85:1094-1103. 1963.-A total of 83 strains of Clostridium perfringens-30 f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.5.1094-1103.1963

    authors: HALL HE,ANGELOTTI R,LEWIS KH,FOTER MJ

    更新日期:1963-05-01 00:00:00

  • Phototaxis and impaired motility in adenylyl cyclase and cyclase receptor protein mutants of Synechocystis sp. strain PCC 6803.

    abstract::We have carefully characterized and reexamined the motility and phototactic responses of Synechocystis sp. adenylyl cyclase (Cya1) and catabolite activator protein (SYCRP1) mutants to different light regimens, glucose, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, and cyclic AMP. We find that contrary to earlier reports, c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00573-06

    authors: Bhaya D,Nakasugi K,Fazeli F,Burriesci MS

    更新日期:2006-10-01 00:00:00

  • Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents.

    abstract::Cook, Thomas M. (Sterling-Winthrop Research Institute, Rensselaer, N.Y.), William H. Deitz, and William A. Goss. Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents. J. Bacteriol. 91:774-779. 1996.-Treatment of Escherichia coli 15TAU with nalidixic acid resu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.2.774-779.1966

    authors: Cook TM,Deitz WH,Goss WA

    更新日期:1966-02-01 00:00:00

  • Purification and characterization of the flagellar hook-basal body complex of Salmonella typhimurium.

    abstract::The hook-basal body complex of Salmonella typhimurium, a major component of its flagellar apparatus, was subjected to detailed analysis by electron microscopy and gel electrophoresis. The study was facilitated by the development of an improved protocol for isolation of the complexes in high yield and purity. Nine prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.3.836-849.1985

    authors: Aizawa SI,Dean GE,Jones CJ,Macnab RM,Yamaguchi S

    更新日期:1985-03-01 00:00:00

  • Chitinolytic activity in Chromobacterium violaceum: substrate analysis and regulation by quorum sensing.

    abstract::Quorum sensing control mediated by N-acyl homoserine lactone (AHL) signaling molecules has been established as a key feature of the regulation of exoenzyme production in many gram-negative bacteria. In Chromobacterium violaceum ATCC 31532 a number of phenotypic characteristics, including production of the purple pigme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4435-4441.1998

    authors: Chernin LS,Winson MK,Thompson JM,Haran S,Bycroft BW,Chet I,Williams P,Stewart GS

    更新日期:1998-09-01 00:00:00

  • Role of the electrochemical proton gradient in genetic transformation of Haemophilus influenzae.

    abstract::The uptake of homologous DNA by Haemophilus influenzae was studied as a function of the proton motive force in completely competent cultures in the pH range of 6 to 8. The composition and magnitude of the proton motive force were varied by using the ionophores valinomycin and nigericin (in the presence of various pota...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.3.868-873.1984

    authors: Bremer W,Kooistra J,Hellingwerf KJ,Konings WN

    更新日期:1984-03-01 00:00:00

  • Dysregulation of Magnesium Transport Protects Bacillus subtilis against Manganese and Cobalt Intoxication.

    abstract::Transition metals are essential for life but are toxic when in excess. Metal ion intoxication may result from the mismetallation of essential metal-dependent enzymes with a noncognate metal. To begin to identify enzymes and processes that are susceptible to mismetallation, we have selected for strains with increased r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00711-19

    authors: Pi H,Wendel BM,Helmann JD

    更新日期:2020-03-11 00:00:00

  • The TolB protein interacts with the porins of Escherichia coli.

    abstract::TolB is a periplasmic protein of the cell envelope Tol complex. It is partially membrane associated through an interaction with the outer membrane lipoprotein PAL (peptidoglycan-associated lipoprotein), which also belongs to the Tol system. The interaction of TolB with outer membrane porins of Escherichia coli was inv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7274-7279.1997

    authors: Rigal A,Bouveret E,Lloubes R,Lazdunski C,Benedetti H

    更新日期:1997-12-01 00:00:00

  • Structural Characterization of the Fla2 Flagellum of Rhodobacter sphaeroides.

    abstract:UNLABELLED:Rhodobacter sphaeroides is a free-living alphaproteobacterium that contains two clusters of functional flagellar genes in its genome: one acquired by horizontal gene transfer (fla1) and one that is endogenous (fla2). We have shown that the Fla2 system is normally quiescent and under certain conditions produc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00170-15

    authors: de la Mora J,Uchida K,del Campo AM,Camarena L,Aizawa S,Dreyfus G

    更新日期:2015-09-01 00:00:00

  • Functional analysis of luxS in the probiotic strain Lactobacillus rhamnosus GG reveals a central metabolic role important for growth and biofilm formation.

    abstract::Quorum sensing is involved in the regulation of multicellular behavior through communication via small molecules. Given the high number and diversity of the gastrointestinal microbiota, it is postulated that members of this community communicate to coordinate a variety of adaptive processes. AI-2 is suggested to be a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01394-06

    authors: Lebeer S,De Keersmaecker SC,Verhoeven TL,Fadda AA,Marchal K,Vanderleyden J

    更新日期:2007-02-01 00:00:00

  • Regulation of chromosome replication in Bacillus subtilis: effects of amino acid starvation in strain W23.

    abstract::Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.2.595-603.1971

    authors: Copeland JC

    更新日期:1971-02-01 00:00:00