Abstract:
:Following arrest by UV-induced DNA damage, replication is restored through a sequence of steps that involve partial resection of the nascent DNA by RecJ and RecQ, branch migration and processing of the fork DNA surrounding the lesion by RecA and RecF-O-R, and resumption of DNA synthesis once the blocking lesion has been repaired or bypassed. In vitro, the primosomal proteins (PriA, PriB, and PriC) and Rep are capable of initiating replication from synthetic DNA fork structures, and they have been proposed to catalyze these events when replication is disrupted by certain impediments in vivo. Here, we characterized the role that PriA, PriB, PriC, and Rep have in processing and restoring replication forks following arrest by UV-induced DNA damage. We show that the partial degradation and processing of the arrested replication fork occurs normally in both rep and primosome mutants. In each mutant, the nascent degradation ceases and DNA synthesis initially resumes in a timely manner, but the recovery then stalls in the absence of PriA, PriB, or Rep. The results demonstrate a role for the primosome and Rep helicase in overcoming replication forks arrested by UV-induced damage in vivo and suggest that these proteins are required for the stability and efficiency of the replisome when DNA synthesis resumes but not to initiate de novo replication downstream of the lesion.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Courcelle CT,Landstrom AJ,Anderson B,Courcelle Jdoi
10.1128/JB.00290-12subject
Has Abstractpub_date
2012-08-01 00:00:00pages
3977-86issue
15eissn
0021-9193issn
1098-5530pii
JB.00290-12journal_volume
194pub_type
杂志文章abstract::The Rhodobacter capsulatus genome contains three genes (olsA [plsC138], plsC316, and plsC3498) that are annotated as lysophosphatidic acid (1-acyl-sn-glycerol-3-phosphate) acyltransferase (AGPAT). Of these genes, olsA was previously shown to be an O-acyltransferase in the second step of ornithine lipid biosynthesis, w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01121-07
更新日期:2007-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00758-06
更新日期:2006-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.184.13.3649-3656.2002
更新日期:2002-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00820-15
更新日期:2016-07-28 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.636-643.1984
更新日期:1984-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01850-07
更新日期:2008-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.270-275.1985
更新日期:1985-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.2.805-807.1984
更新日期:1984-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2016-07-13 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.41-49.1983
更新日期:1983-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.17.5455-5460.1999
更新日期:1999-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.2.455-461.1986
更新日期:1986-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.3.1144-1155.1973
更新日期:1973-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.21.7003-7012.1992
更新日期:1992-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.13.3773-3779.2003
更新日期:2003-07-01 00:00:00
abstract::The gram-positive bacterium Bacillus subtilis contains two minimal Tat translocases, TatAdCd and TatAyCy, which are each involved in the secretion of one or more specific protein substrates. We have investigated the subcellular localization of the TatA components by employing C-terminal green fluorescent protein (GFP)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00215-09
更新日期:2009-07-01 00:00:00
abstract::The ability to form type 1 fimbriae (Fim(+)) was readily transduced to 159 out of 161 wild-type motile Fim(-) FIRN strains of Salmonella typhimurium with phage P22 propagated on a Fim(+) donor strain. Fim(+) clones were isolated from about 35% of tests after the fimbriate bacteria in the transduction mixture had been ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.3.655-658.1971
更新日期:1971-09-01 00:00:00
abstract::The ultrastructure of seven Thiobacillus species was studied. The structure of their cell envelopes is similar, if not identical, to that found in other gram-negative bacteria. Obvious differences were noted in the middle layer of the cell envelope of the seven cultures. Polyhedral inclusion bodies were apparent in fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.2.618-627.1970
更新日期:1970-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.2.340-349.1981
更新日期:1981-08-01 00:00:00
abstract::After treatment with N-methyl-N'-nitro-N-nitrosoguanidine, 133 independent mutants of a haploid strain of Escherichia coli able to use phenyl-beta-galactoside as a carbon source were obtained. The galactoside was specific in selecting for mutants with increases in their uninduced levels of beta-galactosidase. Virtuall...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.500-507.1974
更新日期:1974-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.18.6046-6048.1993
更新日期:1993-09-01 00:00:00
abstract::The operons of the glp regulon encoding the glycerol metabolic enzymes of Pseudomonas aeruginosa were hitherto believed to be positively regulated by the product of the glpR regulatory gene. During nucleotide sequence analysis of the region located upstream of the previously characterized glpD gene, encoding sn-glycer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.17.5215-5221.1996
更新日期:1996-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1260-1266.1987
更新日期:1987-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.2.467-473.1980
更新日期:1980-05-01 00:00:00
abstract::The electron transport system of Neisseria gonorrhoeae was partially characterized by using spectrophotometric, spectroscopic, and oxygen consumption measurements. The effects of selected electron transport inhibitors (amytal, rotenone, 2-heptyl-4-hydroxyquinoline, antimycin A1, and potassium cyanide [KCN]) on electro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.537-545.1978
更新日期:1978-05-01 00:00:00
abstract::Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of informat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01067-07
更新日期:2008-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01695-06
更新日期:2007-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.24.6719-6728.1998
更新日期:1998-12-01 00:00:00
abstract::MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05245-11
更新日期:2011-08-01 00:00:00
abstract::Prolyl-phenylalanine-specific serine protease (dentilisin) is a major extracellular protease produced by Treponema denticola. The gene, prtP, coding for the protease was recently cloned and sequenced (K. Ishihara, T. Miura, H. K. Kuramitsu, and K. Okuda, Infect. Immun. 64:5178-5186, 1996). In order to determine the ro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.15.3837-3844.1998
更新日期:1998-08-01 00:00:00