Abstract:
:The gram-positive bacterium Bacillus subtilis contains two minimal Tat translocases, TatAdCd and TatAyCy, which are each involved in the secretion of one or more specific protein substrates. We have investigated the subcellular localization of the TatA components by employing C-terminal green fluorescent protein (GFP) fusions and fluorescence microscopy. When expressed from a xylose-inducible promoter, the TatA-GFP fusion proteins displayed a dual localization pattern, being localized peripherally and showing bright foci which are predominantly located at the division sites and/or poles of the cells. Importantly, the localization of TatAd-GFP was similar when the protein was expressed from its own promoter under phosphate starvation conditions, indicating that these foci are not the result of artificial overexpression. Moreover, the TatAd-GFP fusion protein was shown to be functional in the translocation of its substrate PhoD, provided that TatCd is also present. Furthermore, we demonstrate that the localization of TatAd-GFP in foci depends on the presence of the TatCd component. Remarkably, however, the TatAd-GFP foci can also be observed in the presence of TatCy, indicating that TatAd can interact not only with TatCd but also with TatCy. These results suggest that the formation of TatAd complexes in B. subtilis is controlled by TatC.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ridder AN,de Jong EJ,Jongbloed JD,Kuipers OPdoi
10.1128/JB.00215-09subject
Has Abstractpub_date
2009-07-01 00:00:00pages
4410-8issue
13eissn
0021-9193issn
1098-5530pii
JB.00215-09journal_volume
191pub_type
杂志文章abstract::D-[alpha-14C]]glucosyl phosphorylpolyprenol ([ 14C]Glc-P-prenol) was formed from UDP-D-[14C]glucose in each of the membrane systems obtained from Bacillus coagulans AHU 1631 and AHU 1634 and two Bacillus megaterium strains. Membranes of these B. coagulans strains, which possess beta-D-glucosyl branches on the repeatin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2835-2841.1989
更新日期:1989-05-01 00:00:00
abstract::Spore cortex of conditional cortexless mutants of Bacillus sphaericus 9602 was not detectable by electron microscopy unless the medium was supplemented with meso-alpha,epsilon-diaminopimelic acid during sporulation. Other spore structures appeared normal. Spore shape was quite irregular in the absence of meso-alpha,ep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.3.1568-1570.1976
更新日期:1976-09-01 00:00:00
abstract::The O antigen of the Shigella flexneri lipopolysaccharide (LPS) is an important virulence determinant and immunogen. We have isolated S. flexneri mutants which produce a semi-rough LPS by using an O-antigen-specific phage, Sf6c. Western immunoblotting was used to show that the LPS produced by the semi-rough mutants co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.733-747.1994
更新日期:1994-02-01 00:00:00
abstract::The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01722-07
更新日期:2008-04-01 00:00:00
abstract::The genes encoding Shiga toxin (stx), the major virulence factor of Shiga toxin-encoding Escherichia coli (STEC) strains, are carried on lambdoid prophages resident in all known STEC strains. The stx genes are expressed only during lytic growth of these temperate bacteriophages. We cloned the gene encoding the repress...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7659-7669.2004
更新日期:2004-11-01 00:00:00
abstract::The invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium is mediated by a type III secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). Expression of the SPI1 T3SS is tightly regulated by the combined action of HilC, HilD, and RtsA, three AraC family members that c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00926-07
更新日期:2008-01-01 00:00:00
abstract::Freter, Rolf (Jefferson Medical College, Philadelphia, Pa.), and Atsushi Ozawa. Explanation for limitation of populations of Escherichia coli in broth cultures. J. Bacteriol. 86:904-910. 1963.-Veal Infusion Broth surrounding a cellophane bag containing a 24-hr culture of Escherichia coli was inhibitory to an inoculum ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.5.904-910.1963
更新日期:1963-11-01 00:00:00
abstract::Synchronized cultures of Anacystis nidulans (Synechococcus PCC 6301), an obligate phototroph, are obtained by incubating exponential cultures in the dark for 12 to 16 h. A temporal and sequential order of macromolecular synthesis is observed within the cell division cycle of a synchronously dividing culture in the lig...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.3.1315-1321.1983
更新日期:1983-03-01 00:00:00
abstract::Enzymes of the ATP-independent Deg serine endopeptidase family are very flexible with regard to their substrate specificity. Some family members cleave only one substrate, while others act as general proteases on unfolded substrates. The proteolytic activity of Deg proteases is regulated by PDZ protein interaction dom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00883-07
更新日期:2007-09-01 00:00:00
abstract:UNLABELLED:The gene encoding a nonoxidative decarboxylase capable of catalyzing the transformation of 2-hydroxy-1-naphthoic acid (2H1NA) to 2-naphthol was identified, recombinantly expressed, and purified to homogeneity. The putative gene sequence of the decarboxylase (hndA) encodes a 316-amino-acid protein (HndA) with...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00250-16
更新日期:2016-05-27 00:00:00
abstract::Selenium is a constituent in Escherichia coli of the anaerobic enzyme formate dehydrogenase in the form of selenocysteine. Selenium is also present in the tRNA of E. coli in the modified base 5-methylaminomethyl-2-selenouracil (mnm5Se2U). The pathways of bacterial selenium metabolism are largely uncharacterized, and i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.2.736-743.1988
更新日期:1988-02-01 00:00:00
abstract::The first step in the transfer of the Bacteroides conjugative transposon CTnDOT is excision of the integrated element from the chromosome to form a circular transfer intermediate. Excision occurs only after the bacteria are exposed to tetracycline. Previously, four excision genes were identified. One was the integrase...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.16.5732-5741.2005
更新日期:2005-08-01 00:00:00
abstract::Many gram-negative bacteria harbor a copper/zinc-containing superoxide dismutase (CuZnSOD) in their periplasms. In pathogenic bacteria, one role of this enzyme may be to protect periplasmic biomolecules from superoxide that is released by host phagocytic cells. However, the enzyme is also present in many nonpathogens ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00554-06
更新日期:2006-09-01 00:00:00
abstract::Listeria monocytogenes mutants with deletions in aroA, aroB, or aroE exhibited strong posttranscriptional upregulation of internalin A (InlA) and InlB synthesis, which resulted in a more-than-10-fold increase in InlA-mediated internalization by epithelial Caco-2 cells and a 4-fold increase in InlB-mediated internaliza...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.8.2836-2845.2005
更新日期:2005-04-01 00:00:00
abstract::In this study, the H303A mutant form of the cbb(3) oxidase (H303A oxidase), which has the H303A mutation in its catalytic subunit (CcoN), was purified from Rhodobacter sphaeroides. The H303A oxidase showed the same catalytic activity as did the wild-type form of the oxidase (WT oxidase). The heme contents of the mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00443-07
更新日期:2007-08-01 00:00:00
abstract::The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.3.1160-1169.1973
更新日期:1973-12-01 00:00:00
abstract::We present the complete genome sequence of Listeria welshimeri, a nonpathogenic member of the genus Listeria. Listeria welshimeri harbors a circular chromosome of 2,814,130 bp with 2,780 open reading frames. Comparative genomic analysis of chromosomal regions between L. welshimeri, Listeria innocua, and Listeria monoc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00758-06
更新日期:2006-11-01 00:00:00
abstract::Interest in finding small RNAs (sRNAs) in bacteria has significantly increased in recent years due to their regulatory functions. Development of high-throughput methods and more sophisticated computational algorithms has allowed rapid identification of sRNA candidates in different species. However, given their various...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02359-14
更新日期:2015-01-01 00:00:00
abstract::Studies on the crisp-1 (cr-1), cyclic adenosine 3',5'-monophosphate (cAMP)-deficient mutants of Neurospora crassa were undertaken to characterize the response of these mutants to exogenous cyclic nucleotides and cyclic nucleotide analogs. A growth tube bioassay and a radioimmune assay for cyclic nucleotides yielded th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1140-1144.1979
更新日期:1979-03-01 00:00:00
abstract::A chemotaxis signal transduction pathway (hereafter called Che1) has been previously identified in the alphaproteobacterium Azospirillum brasilense. Previous experiments have demonstrated that although mutants lacking CheB and/or CheR homologs from this pathway are defective in chemotaxis, a mutant in which the entire...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00734-08
更新日期:2008-10-01 00:00:00
abstract::The function of AI-2 in many bacteria and the physiological role of LuxS, the enzyme responsible for its production, remain matters of debate. Here, we show that in Staphylococcus aureus the luxS gene forms a monocistronic transcriptional unit under the control of a sigma(70)-dependent promoter. The gene was transcrib...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.8.2885-2897.2006
更新日期:2006-04-01 00:00:00
abstract::We recently reported a novel genetic locus located in the sbcB-his region of the chromosomal map of Escherichia coli K-12 which directs the expression of group 6-positive phenotype in Shigella flexneri lipopolysaccharide, presumably due to the transfer of O-acetyl groups onto rhamnose residues of the S. flexneri O-spe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.13.4133-4143.1994
更新日期:1994-07-01 00:00:00
abstract::Two proteins of Escherichia coli, termed Wzc and Wzb, were analyzed for their capacity to participate in the reversible phosphorylation of proteins on tyrosine. First, Wzc was overproduced from its specific gene and purified to homogeneity by affinity chromatography. Upon incubation in the presence of radioactive ATP,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.11.3472-3477.1999
更新日期:1999-06-01 00:00:00
abstract::The nucleotide sequence of the pathogenic spirochete Brachyspira hyodysenteriae bit (for "Brachyspira iron transport") genomic region has been determined. The bit region is likely to encode an iron ATP-binding cassette transport system with some homology to those encountered in gram-negative bacteria. Six open reading...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.22.6948-6957.1999
更新日期:1999-11-01 00:00:00
abstract::Extracellular muramidase-2 of Enterococcus hirae ATCC 9790 was purified to homogeneity by substrate binding, guanidine-HCl extraction, and reversed-phase chromatography. A monoclonal antibody, 2F8, which specifically recognizes muramidase-2, was used to screen a genomic library of E. hirae ATCC 9790 DNA in bacteriopha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.5.1619-1625.1992
更新日期:1992-03-01 00:00:00
abstract::We are engaged in the genetic and physical characterization of an enterotoxin (Ent) plasmid, Ent P307, which contains genes for the production of a hear-labile and a heat-stable enterotoxin. We are using an Escherichia coli K-12 strain, 711 (P307), constructed by S. Falkow, which contains no other plasmids besides Ent...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1240-1247.1975
更新日期:1975-12-01 00:00:00
abstract::A primary determinant of pathogenicity in Erwinia stewartii is the production of extracellular polysaccharide (EPS). A single mutation can abolish both EPS synthesis and pathogenicity; both properties are restored by a single cosmid clone. Subcloning and insertion analysis have defined a single positive regulatory fun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4525-4531.1987
更新日期:1987-10-01 00:00:00
abstract::Energy-coupling factor (ECF) transporters form a large group of vitamin uptake systems in prokaryotes. They are composed of highly diverse, substrate-specific, transmembrane proteins (S units), a ubiquitous transmembrane protein (T unit), and homo- or hetero-oligomeric ABC ATPases. Biotin transporters represent a spec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00350-13
更新日期:2013-09-01 00:00:00
abstract::Proteus mirabilis is a dimorphic bacterium which exists in liquid cultures as a 1.5- to 2.0-microns motile swimmer cell possessing 6 to 10 peritrichous flagella. When swimmer cells are placed on a surface, they differentiate by a combination of events that ultimately produce a swarmer cell. Unlike the swimmer cell, th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.19.6279-6288.1991
更新日期:1991-10-01 00:00:00
abstract::Transport systems for K+ in Escherichia coli are not detectable in membrane vesicles, but vesicles will take up K+ (and Rb+) in the presence of valinomycin. It is generally believed that valinomycin acts as a lipid-soluble cation carrier and that it does not interact with or activate cation transport systems. This vie...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.334-337.1986
更新日期:1986-04-01 00:00:00