Proteus mirabilis mutants defective in swarmer cell differentiation and multicellular behavior.

abstract：:Tn4551, a clindamycin resistance (Ccr) transposon from the R plasmid pBI136, was cloned onto an Escherichia coli-Bacteroides shuttle vector which could replicate normally in E. coli but was maintained unstably in Bacteroides fragilis. To aid in cloning and to ensure maintenance of Tn4551 in E. coli, a kanamycin resist...

journal_title：Journal of bacteriology

authors： Smith CJ,Spiegel H

更新日期：1987-08-01 00:00:00

abstract：:High-resolution electron microscopy of polarly flagellated bacteria revealed that their flagella originate at a circular, differentiated portion of the cytoplasmic membrane approximately 25 nm in diameter. The flagella also have discs attaching them to the cell wall. These attachment discs are extremely resistant to l...

journal_title：Journal of bacteriology

authors： Vaituzis Z,Doetsch RN

更新日期：1969-10-01 00:00:00

abstract：:The neuS-encoded polysialytransferase (polyST) in Escherichia coli K1 catalyzes synthesis of polysialic acid homopolymers composed of unbranched sialyl alpha 2,8 linkages. Subcloning and complementation experiments showed that the K1 neuS was functionally interchangeable with the neuS from E. coli K92 (S. M. Steenberg...

journal_title：Journal of bacteriology

authors： Vimr ER,Bergstrom R,Steenbergen SM,Boulnois G,Roberts I

更新日期：1992-08-01 00:00:00

abstract：:Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...

journal_title：Journal of bacteriology

authors： Jackson AA,Gross MJ,Daniels EF,Hampton TH,Hammond JH,Vallet-Gely I,Dove SL,Stanton BA,Hogan DA

更新日期：2013-07-01 00:00:00

abstract：:Saccharomyces cerevisiae was grown in a continuous culture at a single dilution rate with input ammonia concentrations whose effects ranged from nitrogen limitation to nitrogen excess and glucose limitation. The rate of ammonia assimilation (in millimoles per gram of cells per hour) was approximately constant. Increas...

journal_title：Journal of bacteriology

authors： ter Schure EG,Silljé HH,Verkleij AJ,Boonstra J,Verrips CT

更新日期：1995-11-01 00:00:00

abstract：:Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...

journal_title：Journal of bacteriology

authors： Graumann PL,Losick R

更新日期：2001-07-01 00:00:00

abstract：:We report the draft genome sequence of Paenisporosarcina sp. strain TG-20, which is 4.12 Mb in size and consists of 4,071 protein-coding genes and 76 RNA genes. The genome sequence of Paenisporosarcina sp. TG-20 may provide useful information about molecular adaptations that enhance survival in icy subsurface environm...

journal_title：Journal of bacteriology

authors： Lee JH,Koh HY,Lee SG,Doyle S,Christner BC,Kim HJ

更新日期：2012-12-01 00:00:00

abstract：:Levy, Stuart B. (Institut du Radium, Paris, France). Isologous interference with ultraviolet and X-ray irradiated bacteriophage T(2). J. Bacteriol. 87:1330-1338. 1964.-Qualitative and quantitative analysis of the interference capacity of an irradiated T(2) bacteriophage was made with ultraviolet and X-ray irradiation....

journal_title：Journal of bacteriology

authors： LEVY SB

更新日期：1964-06-01 00:00:00

abstract：:Dental plaque is a complex biofilm that accretes in a series of discrete steps proceeding from a gram-positive streptococcus-rich biofilm to a structure rich in gram-negative anaerobes. This study investigated information flow between two unrelated plaque bacteria, Streptococcus cristatus and Porphyromonas gingivalis....

journal_title：Journal of bacteriology

authors： Xie H,Cook GS,Costerton JW,Bruce G,Rose TM,Lamont RJ

更新日期：2000-12-01 00:00:00

abstract：:Many surface proteins of pathogenic gram-positive bacteria are linked to the cell wall envelope by a mechanism requiring a C-terminal sorting signal with an LPXTG motif. Surface proteins of Streptococcus pneumoniae harbor another motif, YSIRK-G/S, which is positioned within signal peptides. The signal peptides of some...

journal_title：Journal of bacteriology

authors： Bae T,Schneewind O

更新日期：2003-05-01 00:00:00

abstract：:Amber mutants of bacteriophage P1 were used to identify functions involved in late regulation of the P1 lytic growth cycle. A single function has been genetically identified to be involved in activation of the phage-specific late promoter sequence Ps. In vivo, P1 gene 10 amber mutants fail to trans activate a lacZ ope...

journal_title：Journal of bacteriology

authors： Lehnherr H,Guidolin A,Arber W

更新日期：1991-10-01 00:00:00

abstract：:A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...

journal_title：Journal of bacteriology

authors： Berg CM,Liu L,Wang B,Wang MD

更新日期：1988-01-01 00:00:00

abstract：:ruvC mutants of Escherichia coli appear to lack an activity that resolves Holliday intermediates into recombinant products. Yet, these strains produce close to normal numbers of recombinants in genetic crosses. This recombination proficiency was found to be a function of recG. A "mini-kan" insertion in recG was introd...

journal_title：Journal of bacteriology

authors： Lloyd RG

更新日期：1991-09-01 00:00:00

abstract：:The heat shock response of the groESL operon of Agrobacterium tumefaciens was studied at the RNA level. The operon was found to be activated under heat shock conditions and transcribed as a polycistronic mRNA that contains the groES and groEL genes. After activation, the polycistronic mRNA appeared to be cleaved betwe...

journal_title：Journal of bacteriology

authors： Segal G,Ron EZ

更新日期：1995-02-01 00:00:00

abstract：:Genetic exchange mechanisms, to our knowledge, have not been reported for Legionella pneumophila, and consequently, studies on the genetic organization of L. pneumophila have not appeared in the literature. Here, we describe gene transfer mediated by broad host range conjugative plasmids in Legionella spp. Escherichia...

journal_title：Journal of bacteriology

authors： Dreyfus LA,Iglewski BH

更新日期：1985-01-01 00:00:00

abstract：:Margherita, S. S. (Temple University School of Medicine, Philadelphia, Pa.), and H. Friedmann. Induction of nonspecific resistance by endotoxin in unresponsive mice. J. Bacteriol. 89:277-280. 1965.-Serratia marcescens endotoxin was tested for protective effect in animals of varying immunological competence. A quantity...

journal_title：Journal of bacteriology

authors： MARGHERITA SS,FRIEDMAN H

更新日期：1965-02-01 00:00:00

abstract：:Recombinational repair-dependent mutants identify ways to avoid chromosomal lesions. Starting with a recBC(Ts) strain of Escherichia coli, we looked for mutants unable to grow at 42 degrees C in conditions that inactivate the RecBCD(Ts) enzyme. We isolated insertions in ackA and pta, which comprise a two-gene operon r...

journal_title：Journal of bacteriology

authors： Shi IY,Stansbury J,Kuzminov A

更新日期：2005-02-01 00:00:00

abstract：:To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...

journal_title：Journal of bacteriology

authors： Shaw GC,Sung CC,Liu CH,Kao HS

更新日期：1997-01-01 00:00:00

abstract：:We have isolated the gene encoding L-allo-threonine aldolase (L-allo-TA) from Aeromonas jandaei DK-39, a pyridoxal 5'-phosphate (PLP)-dependent enzyme that stereospecifically catalyzes the interconversion of L-allo-threonine and glycine. The gene contains an open reading frame consisting of 1,014 nucleotides correspon...

journal_title：Journal of bacteriology

authors： Liu JQ,Dairi T,Kataoka M,Shimizu S,Yamada H

更新日期：1997-06-01 00:00:00

abstract：:A segment of Escherichia coli DNA that contained a discontinuity of homology with Salmonella typhimurium DNA was isolated. The segment, 1,430 base pairs long, was derived from one end of the lac "loop," a region of about 12 kilobase pairs of E. coli DNA, including the lac operon which has no detectable homology with S...

journal_title：Journal of bacteriology

authors： Buvinger WE,Lampel KA,Bojanowski RJ,Riley M

更新日期：1984-08-01 00:00:00

abstract：:Myxobacteria are predatory and are prolific producers of secondary metabolites. Here, we tested a hypothesized role that secondary metabolite antibiotics function as weapons in predation. To test this, a Myxococcus xanthus Δta1 mutant, blocked in antibiotic TA (myxovirescin) production, was constructed. This TA(-) mut...

journal_title：Journal of bacteriology

authors： Xiao Y,Wei X,Ebright R,Wall D

更新日期：2011-09-01 00:00:00

abstract：:Radiorespirometric and enzymatic analyses reveal that glucose-grown cells of Rhizobium japonicum isolates I-110 and L1-110, both derivatives of R. japonicum strain 3I1b110, possess an active tricarboxylic acid cycle and metabolize glucose by simultaneous operation of the Embden-Meyerhof-Parnas and Entner-Doudoroff pat...

journal_title：Journal of bacteriology

authors： Mulongoy K,Elkan GH

更新日期：1977-07-01 00:00:00

abstract：:Theophylline, aminophylline, and isobutylmethylxanthine, compounds reported to be inhibitors of adenosine 3',5'-phosphate (cAMP) phosphodiesterase, prevented the alpha-factor-induced cell cycle arrest of Saccharomyces cerevisiae a cells. To determine whether the in vivo effect of these methylxanthines on yeast pheromo...

journal_title：Journal of bacteriology

authors： Liao HH,Thorner J

更新日期：1981-12-01 00:00:00

abstract：:top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoilin...

journal_title：Journal of bacteriology

authors： Pruss GJ,Drlica K

更新日期：1985-11-01 00:00:00

abstract：:Chemotactic transducer proteins of Escherichia coli contain four or five methyl-accepting glutamates that are crucial for sensory adaptation and gradient sensing. Two residues arise from posttranslational deamidation of glutamines to yield methyl-accepting glutamates. We addressed the significance of this arrangement ...

journal_title：Journal of bacteriology

authors： Park C,Dutton DP,Hazelbauer GL

更新日期：1990-12-01 00:00:00

abstract：:Ralston, D. J. (University of California, Berkeley) and M. D. Perry. Inhibitory action of phage K on staphylococcal dehydrogenases. II. Its possible relationship to sensitization and cell lysis. J. Bacteriol. 86:740-748. 1963.-By measuring the reduction of the dye triphenyl tetrazolium chloride to the insoluble red fo...

journal_title：Journal of bacteriology

authors： RALSTON DJ,PERRY MD

更新日期：1963-10-01 00:00:00

abstract：:Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1979-08-01 00:00:00

abstract：:We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These mutant strains were selected as glutamine-independent derivatives of a strain that is merodiploid for the glnA region and co...

journal_title：Journal of bacteriology

authors： Rothstein DM,Magasanik B

更新日期：1980-02-01 00:00:00

abstract：:The Alcaligenes eutrophus JMP134 plasmid pJP4 contains genes necessary for the complete degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 3-chlorobenzoic acid. tfdA encodes 2,4-D monooxygenase, the initial enzyme in the 2,4-D catabolic pathway. The tfdA locus has recently been localized to a region on pJP4 13 ...

journal_title：Journal of bacteriology

authors： Perkins EJ,Lurquin PF

更新日期：1988-12-01 00:00:00

abstract：:We have carefully characterized and reexamined the motility and phototactic responses of Synechocystis sp. adenylyl cyclase (Cya1) and catabolite activator protein (SYCRP1) mutants to different light regimens, glucose, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, and cyclic AMP. We find that contrary to earlier reports, c...

journal_title：Journal of bacteriology

authors： Bhaya D,Nakasugi K,Fazeli F,Burriesci MS

更新日期：2006-10-01 00:00:00