Abstract:
:Myxobacteria are predatory and are prolific producers of secondary metabolites. Here, we tested a hypothesized role that secondary metabolite antibiotics function as weapons in predation. To test this, a Myxococcus xanthus Δta1 mutant, blocked in antibiotic TA (myxovirescin) production, was constructed. This TA(-) mutant was defective in producing a zone of inhibition (ZOI) against Escherichia coli. This shows that TA is the major M. xanthus-diffusible antibacterial agent against E. coli. Correspondingly, the TA(-) mutant was defective in E. coli killing. Separately, an engineered E. coli strain resistant to TA was shown to be resistant toward predation. Exogenous addition of spectinomycin, a bacteriostatic antibiotic, rescued the predation defect of the TA(-) mutant. In contrast, against Micrococcus luteus the TA(-) mutant exhibited no defect in ZOI or killing. Thus, TA plays a selective role on prey species. To extend these studies to other myxobacteria, the role of antibiotic corallopyronin production in predation was tested and also found to be required for Corallococcus coralloides killing on E. coli. Next, a role of TA production in myxobacterial fitness was assessed by measuring swarm expansion. Here, the TA(-) mutant had a specific swarm rate reduction on prey lawns, and thus reduced fitness, compared to an isogenic TA(+) strain. Based on these observations, we conclude that myxobacterial antibiotic production can function as a predatory weapon. To our knowledge, this is the first report to directly show a link between secondary metabolite production and predation.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Xiao Y,Wei X,Ebright R,Wall Ddoi
10.1128/JB.05052-11subject
Has Abstractpub_date
2011-09-01 00:00:00pages
4626-33issue
18eissn
0021-9193issn
1098-5530pii
JB.05052-11journal_volume
193pub_type
杂志文章abstract::Phosphatidylglycerol, the most abundant acidic phospholipid in Escherichia coli, is considered to play specific roles in various cellular processes that are essential for cell viability. A null mutation of pgsA, which encodes phosphatidylglycerophosphate synthase, does indeed confer lethality. However, pgsA null mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.19.5418-5425.2002
更新日期:2002-10-01 00:00:00
abstract::Aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (PQQ) as a prosthetic group. Seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with PQQ have been isolated in the facultative methanol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.6.1746-1755.1994
更新日期:1994-03-01 00:00:00
abstract::Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily u...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.8.2535-2547.1999
更新日期:1999-04-01 00:00:00
abstract::Campylobacter jejuni commensally colonizes the cecum of birds. The RacR (reduced ability to colonize) response regulator was previously shown to be important in avian colonization. To explore the means by which RacR and its cognate sensor kinase RacS may modulate C. jejuni physiology and colonization, ΔracR and ΔracS ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06041-11
更新日期:2012-05-01 00:00:00
abstract::Mushin, Rose (University of Melbourne, Victoria, Australia) and Frances M. Ashburner. Gastrointestinal microflora of mutton birds (Puffinus tenuirostris) in relation to "limy" disease. J. Bacteriol. 83:1260-1267. 1962.-The aerobic bacterial intestinal flora of mutton birds, healthy and affected with "limy" disease, wa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1260-1267.1962
更新日期:1962-06-01 00:00:00
abstract::The inducible temperate bacteriophage phi75 and a clear-plaque-forming variant, phi75C1, mediated transduction of a 4.4 X 10(6)-dalton multicopy Bacillus pumilus plasmid, pPL10, at frequencies of 10(-5) to 10(-6) transductants per plaque-forming unit. phi75- and phi75C1-mediated transduction of several chromosome mark...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.3.1029-1032.1977
更新日期:1977-09-01 00:00:00
abstract::Purine nucleoside phosphorylase (EC 2.4.2.1) from Bacillus cereus T was examined at hourly intervals during growth and sporulation. The enzyme has maximal activity in extracts prepared from cells during stages I and II. The activity during exponential growth is only 6.6% of the maximum and that in free spores is only ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.1.33-36.1972
更新日期:1972-07-01 00:00:00
abstract::The age-dependent lectin-binding ability of Rhizobium trifolii 0403 capsular polysaccharide (CPS) was examined by following the development of the capsule and its ability to interact with the white clover lectin trifoliin A. Bacteria grown on agar plates for 3, 5, 7, 14, and 21 days were examined by electron microscop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.145-152.1984
更新日期:1984-07-01 00:00:00
abstract::The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.345-356.1967
更新日期:1967-01-01 00:00:00
abstract::Pediococcus halophilus X-160 which lacks catabolite control by glucose was isolated from nature (soy moromi mash). Wild-type strains, in xylose-glucose medium, utilized glucose preferentially over xylose and showed diauxic growth. With wild-type strain I-13, xylose isomerase activity was not induced until glucose was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.1793-1800.1989
更新日期:1989-04-01 00:00:00
abstract::Gauntt, Charles J. (The University of Texas, Austin), and Royce Z. Lockart, Jr. Inhibition of Mengo virus by interferon. J. Bacteriol. 91:176-182. 1966.-The inhibition of Mengo virus replication in L cells resulting from interferon was studied quantitatively. Interferon was titrated on L cells with Western equine ence...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.176-182.1966
更新日期:1966-01-01 00:00:00
abstract::Escherichia coli VC30 is a temperature-sensitive mutant which is defective in autolysis. Strain VC30 lyses at 30 degrees C when treated with beta-lactam antibiotics or D-cycloserine or when deprived of diaminiopimelic acid. The same treatments inhibit growth of the mutant at 42 degrees C but do not cause lysis. Strain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.385-387.1984
更新日期:1984-07-01 00:00:00
abstract::Gill lamellae from the young fruiting bodies of the basidiomycete Coprinus micaceus possess cytoplasmic particles with cytochemically demonstrable acid phosphatase activity; they are presumed to be lysosomes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.443-446.1973
更新日期:1973-07-01 00:00:00
abstract::Integration host factor (IHF) is a small, basic protein that is needed for efficient recombination of bacteriophage lambda, as well as for other host and viral functions. We have constructed strains in which the two subunits of IHF, encoded by the himA and hip genes of Escherichia coli, are expressed under the control...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.9.4124-4127.1987
更新日期:1987-09-01 00:00:00
abstract::Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells at 30 degrees C but grew as spherical cells at 42 degrees C. The phenotype of one of these rod(Ts) mutants was 88% cotran...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.1143-1158.1978
更新日期:1978-12-01 00:00:00
abstract::On the basis of information available in the literature, gluconate dissimilation in Escherichia coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromato...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.3.941-949.1967
更新日期:1967-03-01 00:00:00
abstract::The ribG gene at the 5' end of the riboflavin operon of Bacillus subtilis and a reading frame at 442 kb on the Escherichia coli chromosome (subsequently designated ribD) show similarity with deoxycytidylate deaminase and with the RIB7 gene of Saccharomyces cerevisiae. The ribG gene of B. subtilis and the ribD gene of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.6.2022-2028.1997
更新日期:1997-03-01 00:00:00
abstract::Defects in DNA mismatch repair (MMR) occur frequently in natural populations of pathogenic and commensal bacteria, resulting in a mutator phenotype. We identified a unique genetic element in Streptococcus pyogenes strain SF370 that controls MMR via a dynamic process of prophage excision and reintegration in response t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01569-07
更新日期:2008-10-01 00:00:00
abstract::The ftsZ gene of Escherichia coli, which lies in a cluster of cell division genes at 2 min on the genetic map, codes for a protein which is thought to play a key role in triggering cell division. Using an ftsZ::lacZ operon fusion, we have studied the transcription of the ftsZ gene under conditions in which cell divisi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1392-1399.1990
更新日期:1990-03-01 00:00:00
abstract::An increase in the apparent molecular weight of the principal outer membrane protein (POMP) of Neisseria gonorrhoeae is associated with introduction of the penB2 genetic marker, which results in low-level, relatively nonspecific antibiotic resistance. Limited proteolysis of the two forms of POMP showed that they had f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.847-851.1980
更新日期:1980-08-01 00:00:00
abstract::A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.436-446.1989
更新日期:1989-01-01 00:00:00
abstract::Aronson, John N. (Arizona State University, Tempe), and Gerald R. Wermus. Effects of m-tyrosine on growth and sporulation of Bacillus species. J. Bacteriol. 90:38-46. 1965.-The aromatic amino acid analogue, dl-beta-(3-hydroxyphenyl)alanine (m-tyrosine), reduced sporulation of a strain of Bacillus subtilis to less than...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.1.38-46.1965
更新日期:1965-07-01 00:00:00
abstract::The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.2.479-486.1968
更新日期:1968-08-01 00:00:00
abstract::Mycobacterium tuberculosis and Mycobacterium smegmatis MutT1, MutT2, MutT3, and Rv3908 (MutT4) enzymes were screened for an antimutator role. Results indicate that both MutT1, in M. tuberculosis and M. smegmatis, and MutT4, in M. smegmatis, have that role. Furthermore, an 8-oxo-guanosine triphosphatase function for Mu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.8.3159-3161.2006
更新日期:2006-04-01 00:00:00
abstract:UNLABELLED:The Pseudomonas aeruginosa cyclic AMP (cAMP)-Vfr system (CVS) is a global regulator of virulence gene expression. Regulatory targets include type IV pili, secreted proteases, and the type III secretion system (T3SS). The mechanism by which CVS regulates T3SS gene expression remains undefined. Single-cell exp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00049-16
更新日期:2016-04-14 00:00:00
abstract::Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Induction and repression of nitrate reductase in Neurospora crassa. J. Bacteriol. 82:898-904. 1961.-Techniques are described for studying induced enzyme formation in Neurospora crassa. The effects of various parameters (time, pH, nitrate concentration, etc.) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.6.898-904.1961
更新日期:1961-12-01 00:00:00
abstract::Expression of the major porin structural genes, ompF and ompC, is influenced by medium osmotic strength and requires the products of two regulatory genes, ompR and envZ. To help define the sites required for the expression of both porin genes, we have used a novel selection to identify mutations that decrease ompF tra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.3.1054-1060.1985
更新日期:1985-06-01 00:00:00
abstract::The function of the essential inner membrane protein (IMP) YidC in Escherichia coli has been studied for a limited number of model IMPs and primarily using targeted approaches. These studies suggested that YidC acts at the level of insertion, folding, and quality control of IMPs, both in the context of the Sec translo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01209-10
更新日期:2011-03-01 00:00:00
abstract::The capsular polysaccharide is a critical virulence factor for group B streptococci associated with human infections, yet little is known about capsule biosynthesis. We detected CMP-Neu5Ac synthetase, the enzyme which activates N-acetylneuraminic acid (Neu5Ac, or sialic acid) for transfer to the nascent capsular polys...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.23.7372-7374.1994
更新日期:1994-12-01 00:00:00
abstract::Superoxide dismutase (SOD) and catalase (CAT) levels were determined for several aerobically grown halophilic vibrios and compared with those found in aerobically grown Escherichia coli K-12. The SOD levels ranged from 25 to 103.6 U/mg of protein for the vibrios compared with 44.6 U/mg of protein for E. coli. The CAT ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.375-380.1978
更新日期:1978-05-01 00:00:00