Activators of the glutamate-dependent acid resistance system alleviate deleterious effects of YidC depletion in Escherichia coli.

Abstract:

:The function of the essential inner membrane protein (IMP) YidC in Escherichia coli has been studied for a limited number of model IMPs and primarily using targeted approaches. These studies suggested that YidC acts at the level of insertion, folding, and quality control of IMPs, both in the context of the Sec translocon and as a separate entity. To further our understanding of YidC's role in IMP biogenesis, we screened a random overexpression library for factors that rescued the growth of cells upon YidC depletion. We found that the overexpression of the GadX and GadY regulators of the glutamate-dependent acid resistance system complemented the growth defect of YidC-depleted cells. Evidence is presented that GadXY overexpression counteracts the deleterious effects of YidC depletion on at least two fronts. First, GadXY prepares the cells for the decrease in respiratory capacity upon the depletion of YidC. Most likely, GadXY-regulated processes reduce the drop in proton-motive force that impairs the fitness of YidC-depleted cells. Second, in GadXY-overproducing cells increased levels of the general chaperone GroEL cofractionate with the inner membranes, which may help to keep newly synthesized inner membrane proteins in an insertion-competent state when YidC levels are limiting.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Yu Z,Bekker M,Tramonti A,Cook GM,van Ulsen P,Scheffers DJ,de Mattos JT,De Biase D,Luirink J

doi

10.1128/JB.01209-10

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

1308-16

issue

6

eissn

0021-9193

issn

1098-5530

pii

JB.01209-10

journal_volume

193

pub_type

杂志文章
  • Global analysis of cellular factors and responses involved in Pseudomonas aeruginosa resistance to arsenite.

    abstract::The impact of arsenite [As(III)] on several levels of cellular metabolism and gene regulation was examined in Pseudomonas aeruginosa. P. aeruginosa isogenic mutants devoid of antioxidant enzymes or defective in various metabolic pathways, DNA repair systems, metal storage proteins, global regulators, or quorum sensing...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.14.4853-4864.2005

    authors: Parvatiyar K,Alsabbagh EM,Ochsner UA,Stegemeyer MA,Smulian AG,Hwang SH,Jackson CR,McDermott TR,Hassett DJ

    更新日期:2005-07-01 00:00:00

  • Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion.

    abstract::Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.284-296.1962

    authors: ELLNER PD,BOHAN CD

    更新日期:1962-02-01 00:00:00

  • Instability of pathogenicity islands in uropathogenic Escherichia coli 536.

    abstract::The uropathogenic Escherichia coli strain 536 carries at least five genetic elements on its chromosome that meet all criteria characteristic of pathogenicity islands (PAIs). One main feature of these distinct DNA regions is their instability. We applied the so-called island-probing approach and individually labeled al...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.10.3086-3096.2004

    authors: Middendorf B,Hochhut B,Leipold K,Dobrindt U,Blum-Oehler G,Hacker J

    更新日期:2004-05-01 00:00:00

  • Regulation of the Escherichia coli tna operon: nascent leader peptide control at the tnaC stop codon.

    abstract::Expression of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and by tryptophan-induced transcription antitermination at Rho-dependent termination sites in the leader region of the operon. Tryptophan induction is dependent on translation of a short leader peptide coding region,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.5.1774-1779.1997

    authors: Konan KV,Yanofsky C

    更新日期:1997-03-01 00:00:00

  • uvrC gene function in excision repair in toluene-treated Escherichia coli.

    abstract::We have examined the role of the uvrC gene in UV excision repair by studying incision, excision, repair synthesis, and DNA strand reformation in Escherichia coli mutants made permeable to nucleoside triphosphates by toluene treatment. After irradiation, incisions occur normally in uvrC cells in the presence of nicotin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.397-408.1979

    authors: Sharma S,Moses RE

    更新日期:1979-01-01 00:00:00

  • Heterotrophic bacteria from cultures of autotrophic Thiobacillus ferrooxidans: relationships as studied by means of deoxyribonucleic acid homology.

    abstract::From several presumably pure cultures of Thiobacillus ferrooxidans, we isolated a pair of stable phenotypes. One was a strict autotroph utilizing sulfur or ferrous iron as the energy source and unable to utilize glucose; the other phenotype was an acidophilic obligate heterotroph capable of utilizing glucose but not s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.1.448-454.1980

    authors: Harrison AP Jr,Jarvis BW,Johnson JL

    更新日期:1980-07-01 00:00:00

  • Importance of Individual Germination Receptor Subunits in the Cooperative Function between GerA and Ynd.

    abstract::Germination of Bacillus spores is triggered by the binding of specific nutrients to germinant receptors (GRs) located in the spore's inner membrane. The GRs typically consist of A, B, and C subunits, encoded by tricistronic ger operons. The Bacillus licheniformis genome contains the gerA family operons gerA, ynd, and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00451-19

    authors: Aspholm M,Borch-Pedersen K,O'Sullivan K,Fjellheim S,Aardal IB,Granum PE,Lindbäck T

    更新日期:2019-10-04 00:00:00

  • Replication of the bacteriocinogenic plasmid Clo DF13 in thermosensitive Escherichia coli mutants defective in initiation or elongation of deoxyribonucleic acid replication.

    abstract::The replication of the bacteriocinogenic plasmid Clo DF13 has been studied in the seven temperature-sensitive Escherichia coli mutants defective in deoxyribonucleic acid (DNA) replication (dnaA-dnaG). Experiments with dna initiation mutants revealed that the replication of the Clo DF13 plasmid depends to a great exten...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.3.1227-1237.1974

    authors: Veltkamp E,Nijkamp HJ

    更新日期:1974-12-01 00:00:00

  • Crystal structure of the cystic fibrosis transmembrane conductance regulator inhibitory factor Cif reveals novel active-site features of an epoxide hydrolase virulence factor.

    abstract::Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01348-09

    authors: Bahl CD,Morisseau C,Bomberger JM,Stanton BA,Hammock BD,O'Toole GA,Madden DR

    更新日期:2010-04-01 00:00:00

  • Identification of [2Fe-2S] clusters in microbial ferrochelatases.

    abstract::The terminal enzyme of heme biosynthesis, ferrochelatase (EC 4.99.1.1), catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme. Prior to the present work, [2Fe-2S] clusters have been identified and characterized in animal ferrochelatases but not in plant or prokaryotic ferrochelatases. Herein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.9.2460-2464.2002

    authors: Dailey TA,Dailey HA

    更新日期:2002-05-01 00:00:00

  • Microarray identification of Clostridium difficile core components and divergent regions associated with host origin.

    abstract::Clostridium difficile is a gram-positive, spore-forming enteric anaerobe which can infect humans and a wide variety of animal species. Recently, the incidence and severity of human C. difficile infection has markedly increased. In this study, we evaluated the genomic content of 73 C. difficile strains isolated from hu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00222-09

    authors: Janvilisri T,Scaria J,Thompson AD,Nicholson A,Limbago BM,Arroyo LG,Songer JG,Gröhn YT,Chang YF

    更新日期:2009-06-01 00:00:00

  • Replication control mutations of plasmid R6-5 and their effects on interactions of the RNA-I control element with its target.

    abstract::Nine high copy number mutations of plasmid R6-5, representing five phenotypically distinct groups, have been identified by DNA sequencing. In each mutant plasmid examined, a single nucleotide change was found. The effects of the mutations on possible gene products, and DNA-RNA secondary structure, were analyzed and co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.1.429-436.1983

    authors: Brady G,Frey J,Danbara H,Timmis KN

    更新日期:1983-04-01 00:00:00

  • Sequence, regulation, and functions of fis in Salmonella typhimurium.

    abstract::The fis operon from Salmonella typhimurium has been cloned and sequenced, and the properties of Fis-deficient and Fis-constitutive strains were examined. The overall fis operon organization in S. typhimurium is the same as that in Escherichia coli, with the deduced Fis amino acid sequences being identical between both...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.8.2021-2032.1995

    authors: Osuna R,Lienau D,Hughes KT,Johnson RC

    更新日期:1995-04-01 00:00:00

  • Preferential selection of deletion mutations of the outer membrane lipoprotein gene of Escherichia coli by globomycin.

    abstract::Globomycin is an antibiotic which inhibits the processing of the prolipoprotein. Eighty globomycin-resistant mutants were independently isolated from Escherichia coli K-12 which had a deletion mutation in chromosomal lipoprotein gene (lpp), but contained a plasmid carrying the wild-type lpp gene. Twenty-six of the mut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.1.654-656.1981

    authors: Zwiebel LJ,Inukai M,Nakamura K,Inouye M

    更新日期:1981-01-01 00:00:00

  • Immunochemistry of the Group-Specific Polysaccharide of Nocardia brasiliensis.

    abstract::Estrada-Parra, Sergio (Escuela Nacional de Ciencias Biológicas, México, D.F., México), Abel Zamora, and L. F. Bojalil. Immunochemistry of the group-specific polysaccharide of Nocardia brasiliensis. J. Bacteriol. 90:571-574. 1965.-The group-specific polysaccharide of Nocardia brasiliensis was further purified, yielding...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.3.571-574.1965

    authors: Estrada-Parra S,Zamora A,Bojalil LF

    更新日期:1965-09-01 00:00:00

  • Organization and transcriptional regulation of the Escherichia coli K-12 D-serine tolerance locus.

    abstract::We have reinvestigated the genetic organization and the transcription regulation of the dsd operon of Escherichia coli. By combining genetic and biochemical studies, it is demonstrated that the regulatory region of the operon and the gene encoding the specific regulator of D-serine tolerance (dsdC) had been misplaced ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.22.6456-6461.1995

    authors: Nørregaard-Madsen M,McFall E,Valentin-Hansen P

    更新日期:1995-11-01 00:00:00

  • Pediococcus cerevisiae mutant with altered transport of folates.

    abstract::A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent proces...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.2.400-406.1975

    authors: Mandelbaum-Shavit F,Grossowicz N

    更新日期:1975-08-01 00:00:00

  • Identification of crucial amino acids of bacterial Peptide deformylases affecting enzymatic activity in response to oxidative stress.

    abstract::Peptide deformylase (PDF) is an essential bacterial metalloprotease involved in deformylation of N-formyl group from nascent polypeptide chains during protein synthesis. Iron-containing variants of this enzyme from Salmonella enterica serovar Typhimurium (sPDF) and Mycobacterium tuberculosis (mPDF), although inhibited...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00916-13

    authors: Kumar S,Kanudia P,Karthikeyan S,Chakraborti PK

    更新日期:2014-01-01 00:00:00

  • Identification of multiple repressor recognition sites in the hut system of Pseudomonas putida.

    abstract::The hutC gene in Pseudomonas putida encodes a repressor protein that negatively regulates the expression of all hut genes. We have overexpressed this cloned hutC gene in Escherichia coli to identify P. putida hut regions that could specifically bind the repressor. Ten restriction fragments, some of which were partiall...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.8.4189-4195.1989

    authors: Hu L,Allison SL,Phillips AT

    更新日期:1989-08-01 00:00:00

  • BIOLOGY OF BUDDING BACTERIA. 3. FINE STRUCTURE OF RHODOMICROBIUM AND HYPHOMICROBIUM SPP.

    abstract::Conti, S. F. (Dartmouth Medical School, Hanover, N.H.), and Peter Hirsch. Biology of budding bacteria. III. Fine structure of Rhodomicrobium and Hyphomicrobium spp. J. Bacteriol. 89:503-512. 1965.-The ultrastructure of 14 strains of hyphomicrobia, and of Rhodomicrobium vannielii, was investigated by means of electron ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.2.503-512.1965

    authors: CONTI SF,HIRSCH P

    更新日期:1965-02-01 00:00:00

  • Genetics and biochemistry of pyrimidine biosynthesis in Bacillus subtilis: linkage between mutations resulting in a requirement for uracil.

    abstract::A number of independently derived uracil-requiring mutant strains of the parent strain 168 were mapped by inter-mutant transformation crosses. Only a few of these mutant strains were found to be transformable. Studies were performed in which these transformable uracil-requiring mutant strains were used as the recipien...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.577-581.1973

    authors: Kelleher RJ Jr,Gooder H

    更新日期:1973-11-01 00:00:00

  • The Haemophilus influenzae dprABC genes constitute a competence-inducible operon that requires the product of the tfoX (sxy) gene for transcriptional activation.

    abstract::We previously showed that dprA is required for efficient processing of linear DNA during cellular transformation in Haemophilus influenzae. In this study the transcriptional regulation of dprA and two downstream genes, dprB and dprC, is examined. We demonstrate by Northern blot analysis that the dprABC genes are trans...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.15.4815-4820.1997

    authors: Karudapuram S,Barcak GJ

    更新日期:1997-08-01 00:00:00

  • Control of poly-beta-hydroxybutyrate synthase mediated by acetyl phosphate in cyanobacteria.

    abstract::Poly-beta-hydroxybutyrate (PHB) synthesis in a cyanobacterium, Synechococcus sp. strain MA19, is controlled at the enzyme level and is dependent on the C/N balance in the culture medium. The control involves at least two enzymes. The first enzyme is PHB synthase. Little PHB synthase activity was detected in crude extr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.16.5009-5013.1997

    authors: Miyake M,Kataoka K,Shirai M,Asada Y

    更新日期:1997-08-01 00:00:00

  • Transduction with integration-defective mutants of Salmonella typhimurium bacteriophage KB1.

    abstract::Phage KB1 has gained use as a generalized transducing phage for Salmonella typhimurium, including strains resistant to phage P22. Integration-defective mutants of KB1 have now been isolated; one of these, int-1, is recommended for transduction when nonlysogenic recombinants are desired. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.2.907-908.1974

    authors: McIntire SA

    更新日期:1974-02-01 00:00:00

  • Conjugative plasmids of enteric bacteria from many different incompatibility groups have similar genes for single-stranded DNA-binding proteins.

    abstract::Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.235-241.1985

    authors: Golub EI,Low KB

    更新日期:1985-04-01 00:00:00

  • Facile and gentle method for quantitative lysis of Escherichia coli and Salmonella typhimurium.

    abstract::Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and Rz) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Ind...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.1.354-356.1984

    authors: Crabtree S,Cronan JE Jr

    更新日期:1984-04-01 00:00:00

  • Cloning of the C-terminal cytoplasmic fragment of the tar protein and effects of the fragment on chemotaxis of Escherichia coli.

    abstract::A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies. The C-terminal fragments from wild-type and mutant tar genes were chara...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.6.2521-2526.1988

    authors: Oosawa K,Mutoh N,Simon MI

    更新日期:1988-06-01 00:00:00

  • Lysis of Vibrio succinogenes by ethylenediamine-tetraacetic acid or lysozyme.

    abstract::Wolin, M. J. (University of Illinois, Urbana). Lysis of Vibrio succinogenes by ethylenediaminetetraacetic acid or lysozyme. J. Bacteriol. 91:1781-1786. 1966.-Cell suspensions of Vibrio succinogenes are lysed by ethylenediaminetetraacetic acid (EDTA) or lysozyme. Lysis occurs at alkaline pH and is prevented by 0.15 m N...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.5.1781-1786.1966

    authors: Wolin MJ

    更新日期:1966-05-01 00:00:00

  • Constricted flux through the branched-chain amino acid biosynthetic enzyme acetolactate synthase triggers elevated expression of genes regulated by rpoS and internal acidification.

    abstract::The first common enzyme of isoleucine and valine biosynthesis, acetolactate synthase (ALS), is specifically inhibited by the herbicide sulfometuron methyl (SM). To further understand the physiological consequences of flux alterations at this point in metabolism, Escherichia coli genes whose expression was induced by p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.4.785-792.1998

    authors: Van Dyk TK,Ayers BL,Morgan RW,Larossa RA

    更新日期:1998-02-01 00:00:00

  • Biochemical characterization of StyAB from Pseudomonas sp. strain VLB120 as a two-component flavin-diffusible monooxygenase.

    abstract::Pseudomonas sp. VLB120 uses styrene as a sole source of carbon and energy. The first step in this metabolic pathway is catalyzed by an oxygenase (StyA) and a NADH-flavin oxidoreductase (StyB). Both components have been isolated from wild-type Pseudomonas strain VLB120 as well as from recombinant Escherichia coli. StyA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.16.5292-5302.2004

    authors: Otto K,Hofstetter K,Röthlisberger M,Witholt B,Schmid A

    更新日期:2004-08-01 00:00:00