Replication of the bacteriocinogenic plasmid Clo DF13 in thermosensitive Escherichia coli mutants defective in initiation or elongation of deoxyribonucleic acid replication.

abstract：:Functional domains in the RepI replication initiator protein have been identified by classical and site-directed mutagenesis techniques. Mutations conferring an increase in plasmid copy number contained alterations in a key position of a putative helix-turn-helix DNA binding motif. The mutations did not appear to affe...

journal_title：Journal of bacteriology

authors： Gammie AE,Tolmasky ME,Crosa JH

更新日期：1993-06-01 00:00:00

abstract：:The addition of 5',5',5'-trifluoroleucine (fluoroleucine) to leucine auxotrophs of Salmonella typhimurium permitted protein but not ribonucleic acid (RNA) synthesis to continue after leucine depletion. The uncoupling of the formation of these macromolecules by fluoroleucine was apparent if RNA and protein synthesis wa...

journal_title：Journal of bacteriology

authors： Trela JM,Freundlich M

更新日期：1969-07-01 00:00:00

abstract：:A sequenced collection of plasmid-borne random fusions of Escherichia coli DNA to a Photorhabdus luminescens luxCDABE reporter was used as a starting point to select a set of 689 nonredundant functional gene fusions. This group, called LuxArray 1.0, represented 27% of the predicted transcriptional units in E. coli. Hi...

journal_title：Journal of bacteriology

authors： Van Dyk TK,DeRose EJ,Gonye GE

更新日期：2001-10-01 00:00:00

abstract：:Identification of new genes involved in biofilm formation is needed to understand the molecular basis of strain variation and the pathogenic mechanisms implicated in chronic staphylococcal infections. A biofilm-producing Staphylococcus aureus isolate was used to generate biofilm-negative transposon (Tn917) insertion m...

journal_title：Journal of bacteriology

authors： Cucarella C,Solano C,Valle J,Amorena B,Lasa I,Penadés JR

更新日期：2001-05-01 00:00:00

abstract：:The protein compositions of the membrane preparations from Selenomonas ruminantium grown in glucose or lactate medium were determined by sodium dodecyl sulfate- and two-dimensional (first, isoelectric focusing; second, sodium dodecyl sulfate) polyacrylamide slab gel electrophoresis. The outer membrane from both glucos...

journal_title：Journal of bacteriology

authors： Kamio Y,Takahashi H

更新日期：1980-02-01 00:00:00

abstract：:Glutathione and soluble thiol content were examined in a broad spectrum of bacteria. Significant soluble thiol was present in all cases. The thiol compound was glutathione in most of the gram-negative bacteria but not in most of the gram-positive bacteria studied. Glutathione was absent in four anerobes and one microa...

journal_title：Journal of bacteriology

authors： Fahey RC,Brown WC,Adams WB,Worsham MB

更新日期：1978-03-01 00:00:00

abstract：:The antimicrobial metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) contributes to the capacity of Pseudomonas fluorescens strain CHA0 to control plant diseases caused by soilborne pathogens. A 2, 4-DAPG-negative Tn5 insertion mutant of strain CHA0 was isolated, and the nucleotide sequence of the 4-kb genomic DNA regio...

journal_title：Journal of bacteriology

authors： Schnider-Keel U,Seematter A,Maurhofer M,Blumer C,Duffy B,Gigot-Bonnefoy C,Reimmann C,Notz R,Défago G,Haas D,Keel C

更新日期：2000-03-01 00:00:00

abstract：:The DosS (DevS) and DosT histidine kinases form a two-component system together with the DosR (DevR) response regulator in Mycobacterium tuberculosis. DosS and DosT, which have high sequence similarity to each other over the length of their amino acid sequences, contain two GAF domains (GAF-A and GAF-B) in their N-ter...

journal_title：Journal of bacteriology

authors： Kim MJ,Park KJ,Ko IJ,Kim YM,Oh JI

更新日期：2010-10-01 00:00:00

abstract：:Elongation factor Tu (EF-Tu), encoded by tuf genes, carries aminoacyl-tRNA to the ribosome during protein synthesis. Duplicated tuf genes (tufA and tufB), which are commonly found in enterobacterial species, usually coevolve via gene conversion and are very similar to one another. However, sequence analysis of tuf gen...

journal_title：Journal of bacteriology

authors： Isabel S,Leblanc E,Boissinot M,Boudreau DK,Grondin M,Picard FJ,Martel EA,Parham NJ,Chain PS,Bader DE,Mulvey MR,Bryden L,Roy PH,Ouellette M,Bergeron MG

更新日期：2008-11-01 00:00:00

abstract：:Small subunit rDNA sequences were determined for 20 species of the genera Acetogenium, Clostridium, Thermoanaerobacter, Thermoanaerobacterium, Thermoanaerobium, and Thermobacteroides, 3 non-validly described species, and 5 isolates of anaerobic thermophilic bacteria, providing a basis for a phylogenetic analysis of th...

journal_title：Journal of bacteriology

authors： Rainey FA,Ward NL,Morgan HW,Toalster R,Stackebrandt E

更新日期：1993-08-01 00:00:00

abstract：:Polyamines are present in high concentrations in archaea, yet little is known about their synthesis, except by extrapolation from bacterial and eucaryal systems. S-Adenosylmethionine (AdoMet) decarboxylase, a pyruvoyl group-containing enzyme that is required for spermidine biosynthesis, has been previously identified ...

journal_title：Journal of bacteriology

authors： Kim AD,Graham DE,Seeholzer SH,Markham GD

更新日期：2000-12-01 00:00:00

abstract：:The segregation of mating loci with other unselected genes was analyzed in recombinants obtained from matings of Nocardia canicruria and N. erythropolis. The loci C/c and E/e control nocardial compatibility. Four mating genotype combinations were observed: cE, Ce, CE, and ce. Strains of N. erythropolis bear the genoty...

journal_title：Journal of bacteriology

authors： Brownell GH,Kelly KL

更新日期：1969-07-01 00:00:00

abstract：:In Escherichia coli K-12 mutants which had a new nalidixic acid resistance mutation at about 82 min on the chromosome map, cell growth was resistant to or hypersusceptible to nalidixic acid, oxolinic acid, piromidic acid, pipemidic acid, and novobiocin. Deoxyribonucleic acid gyrase activity as tested by supercoiling o...

journal_title：Journal of bacteriology

authors： Yamagishi J,Furutani Y,Inoue S,Ohue T,Nakamura S,Shimizu M

更新日期：1981-11-01 00:00:00

abstract：:Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translatio...

journal_title：Journal of bacteriology

authors： Sun Y,Vanderpool CK

更新日期：2011-01-01 00:00:00

abstract：:The interaction between endonuclease V, the cyclobutane pyrimidine dimer-specific N-glycosylase/abasic lyase from bacteriophage T4, and DNA was investigated by DNase I footprinting methods. The catalytically inactive mutant E23Q was found to interact with a smaller region of DNA at the abasic site analog, tetrahydrofu...

journal_title：Journal of bacteriology

authors： Latham KA,Manuel RC,Lloyd RS

更新日期：1995-09-01 00:00:00

abstract：:Ornithine carbamoyltransferase synthesis is subject to two major regulatory systems in Saccharomyces cerevisiae. One system is specific for the arginine biosynthetic enzymes, whereas the other appears to be general, acting on a variety of other amino acid pathways as well. We observed that the synthetic capacity for c...

journal_title：Journal of bacteriology

authors： Messenguy F,Cooper TG

更新日期：1977-06-01 00:00:00

abstract：:Overexpression of evgA, a response regulator of a two-component system, increased multidrug efflux in Escherichia coli. Since overexpression of the emrKY operon, which is controlled by evgAS, could account only for deoxycholate resistance, the evgAS locus apparently controls expression of at least one other multidrug ...

journal_title：Journal of bacteriology

authors： Nishino K,Yamaguchi A

更新日期：2001-02-01 00:00:00

abstract：:A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. ...

journal_title：Journal of bacteriology

authors： Eddy CK,Smith OH,Noel KD

更新日期：1988-07-01 00:00:00

abstract：:Periplasmic membrane fusion proteins (MFPs) are essential components of multidrug efflux pumps and type I protein secretion systems of gram-negative bacteria. Located in the periplasm, MFPs function by creating a physical link between inner membrane transporters and outer membrane channels. The most conserved sequence...

journal_title：Journal of bacteriology

authors： Ge Q,Yamada Y,Zgurskaya H

更新日期：2009-07-01 00:00:00

abstract：:Chromatin immunoprecipitation and microarray (ChIP-chip) analysis showed that the nitric oxide (NO)-sensitive repressor NsrR from Escherichia coli binds in vivo to the promoters of the tynA and feaB genes. These genes encode the first two enzymes of a pathway that is required for the catabolism of phenylethylamine (PE...

journal_title：Journal of bacteriology

authors： Rankin LD,Bodenmiller DM,Partridge JD,Nishino SF,Spain JC,Spiro S

更新日期：2008-09-01 00:00:00

abstract：:Succinyl-coenzyme A synthetase of Escherichia coli has an alpha 2 beta 2 subunit structure. By measuring reconstituted enzyme activity present after addition of purified alpha or beta subunits to cell extracts followed by refolding, we have shown that extracts contain no significant excess of either subunit species. T...

journal_title：Journal of bacteriology

authors： Wolodko WT,Brownie ER,Bridger WA

更新日期：1980-07-01 00:00:00

abstract：:Production of bacterial flagella is controlled by a multitiered regulatory system that coordinates the expression of 40 to 50 subunits and ordered assembly of these elaborate structures. Flagellar expression is environmentally controlled, presumably to optimize the benefits and liabilities of having these organelles o...

journal_title：Journal of bacteriology

authors： Markel E,Dalenberg H,Monteil CL,Vinatzer BA,Swingle B

更新日期：2018-03-12 00:00:00

abstract：:This article contains an analysis of a version of the well-known inhibitor-dilution model for the control of initiation of chromosome replication in bacteria. According to this model, an unstable inhibitor interacts with an initiation primer in a hit-and-destroy fashion to prevent successful initiation; both constitue...

journal_title：Journal of bacteriology

authors： Margalit H,Grover NB

更新日期：1987-11-01 00:00:00

abstract：:Vibrio cholerae uses a variety of strategies for obtaining iron in its diverse environments. In this study we report the identification of a novel iron utilization protein in V. cholerae, VciB. The vciB gene and its linked gene, vciA, were isolated in a screen for V. cholerae genes that permitted growth of an Escheric...

journal_title：Journal of bacteriology

authors： Mey AR,Wyckoff EE,Hoover LA,Fisher CR,Payne SM

更新日期：2008-09-01 00:00:00

abstract：:Pigmentation mutants of Pseudomonas aeruginosa, selected by observed visual differences in coloration from the wild-type strain, were examined for altered patterns of phenazine synthesis. Three classes of mutants that were incapable of pyocyanine production were identified. Pigmentation patterns that were found to cha...

journal_title：Journal of bacteriology

authors： Byng GS,Eustice DC,Jensen RA

更新日期：1979-06-01 00:00:00

abstract：:Comprehensive analysis of genome-wide expression patterns during growth of the hyperthermophilic bacterium Thermotoga maritima on 14 monosaccharide and polysaccharide substrates was undertaken with the goal of proposing carbohydrate specificities for transport systems and putative transcriptional regulators. Saccharid...

journal_title：Journal of bacteriology

authors： Conners SB,Montero CI,Comfort DA,Shockley KR,Johnson MR,Chhabra SR,Kelly RM

更新日期：2005-11-01 00:00:00

abstract：:Median longitudinal sections of germ tube apices of Mucor rouxii revealed the presence of a single, roughly hemispherical, electron-dense organelle, in intimate contact with the apical cell wall. Conceivably, this "apical corpuscle" may be responsible for the emission of the germ tube and its continued apical growth. ...

journal_title：Journal of bacteriology

authors： Bartnicki-Garcia S,Nelson N,Cota-Robles E

更新日期：1968-06-01 00:00:00

abstract：:The prevalence of the Moraxella catarrhalis immunoglobulin D (IgD)-binding outer membrane protein MID and its gene was determined in 91 clinical isolates and in 7 culture collection strains. Eighty-four percent of the clinical Moraxella strains expressed MID-dependent IgD binding. The mid gene was detected in all stra...

journal_title：Journal of bacteriology

authors： Möllenkvist A,Nordström T,Halldén C,Christensen JJ,Forsgren A,Riesbeck K

更新日期：2003-04-01 00:00:00

abstract：:Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share ...

journal_title：Journal of bacteriology

authors： Sutcliffe J,Blumenthal R,Walter A,Foulds J

更新日期：1983-11-01 00:00:00

abstract：:Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type w...

journal_title：Journal of bacteriology

authors： Scott WA

更新日期：1977-06-01 00:00:00