Abstract:
:Glutathione and soluble thiol content were examined in a broad spectrum of bacteria. Significant soluble thiol was present in all cases. The thiol compound was glutathione in most of the gram-negative bacteria but not in most of the gram-positive bacteria studied. Glutathione was absent in four anerobes and one microaerophile but was present in a blue-green bacterium. The glutathione content of Escherichia coli increased significantly during transition from exponential to stationary phase.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Fahey RC,Brown WC,Adams WB,Worsham MBdoi
10.1128/JB.133.3.1126-1129.1978subject
Has Abstractpub_date
1978-03-01 00:00:00pages
1126-9issue
3eissn
0021-9193issn
1098-5530journal_volume
133pub_type
杂志文章abstract::The activities of DNA methyltransferases are important for a variety of cellular functions in bacteria. In this study, we developed a modified high-throughput technique called methyl homopolymer tail mediated sequencing (methyl HTM-seq) to identify the undermethylated sites in the Vibrio cholerae genome for the two DN...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02112-12
更新日期:2013-05-01 00:00:00
abstract::The 2-aminoethylphosphonate transaminase (AEPT; the phnW gene product) of the Salmonella enterica serovar Typhimurium 2-aminoethylphosphonate (AEP) degradation pathway catalyzes the reversible reaction of AEP and pyruvate to form phosphonoacetaldehyde (P-Ald) and L-alanine (L-Ala). Here, we describe the purification a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.15.4134-4140.2002
更新日期:2002-08-01 00:00:00
abstract::Daniel, John W. (University of Wisconsin, Madison), and Karlee Babcock. Methionine metabolism of the myxomycete Physarum polycephalum. J. Bacteriol. 92:1028-1035. 1966.-Previous studies have shown that Physarum polycephalum requires exogenous methionine for growth, but not cysteine, folic acid, or vitamin B(12). Methi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.4.1028-1035.1966
更新日期:1966-10-01 00:00:00
abstract::Constitutive beta-glucosidases from Saccharomyces fragilis (Y-18) and S. dobzhanskii (Y-19) precipitated at the same concentration of ammonium sulfate. The partially purified enzymes had similar activation energies, molecular weights, affinities for certain natural and synthetic beta-glucosides, and optimal pH values ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.135-141.1967
更新日期:1967-01-01 00:00:00
abstract::When incubated in the light under 100% oxygen, wild-type blue-green algae (Anacystis nidulans, Synechococcus cedrorum) die out rapidly at temperatures of 4 to 15 C, and at 35 C (or at 26 C in the case of S. cedrorum) in the absence of CO(2). Photosynthesis is impaired in these cells long before they die. Blocking of p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.3.682-689.1972
更新日期:1972-09-01 00:00:00
abstract::The energy source shift-down described in the preceding paper (Molin et al., J. Bacteriol. 131: 7-17, 1977) was used to study the effects of shift-down on protein synthesis. The overall rate of protein synthesis was reduced immediately, and to the same extent, in stringent and relaxed strains. The primary effect of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.1.18-29.1977
更新日期:1977-07-01 00:00:00
abstract:UNLABELLED:The role of chromosomal toxin-antitoxin (TA) systems, which are ubiquitous within the genomes of free-living bacteria, is still debated. We have scanned the Vibrio cholerae N16961 genome for class 2 TA genes and identified 18 gene pair candidates. Interestingly, all but one are located in the chromosome 2 su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00108-15
更新日期:2015-07-01 00:00:00
abstract::Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harbor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.22.7136-7141.1991
更新日期:1991-11-01 00:00:00
abstract::In this study, we show that Salmonella produces an O-antigen capsule coregulated with the fimbria- and cellulose-associated extracellular matrix. Structural analysis of purified Salmonella extracellular polysaccharides yielded predominantly a repeating oligosaccharide unit similar to that of Salmonella enterica serova...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00809-06
更新日期:2006-11-01 00:00:00
abstract::The interaction between endonuclease V, the cyclobutane pyrimidine dimer-specific N-glycosylase/abasic lyase from bacteriophage T4, and DNA was investigated by DNase I footprinting methods. The catalytically inactive mutant E23Q was found to interact with a smaller region of DNA at the abasic site analog, tetrahydrofu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.17.5166-5168.1995
更新日期:1995-09-01 00:00:00
abstract::The vitamin requirements of the group Q streptococci (Streptococcus avium) and the established enterococcal species (S. faecalis and S. faecium) paralleled one another, although S. avium did not characteristically require riboflavine or pyridoxal. S. avium was further differentiated in that it required thymine for gro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.2.297-299.1967
更新日期:1967-08-01 00:00:00
abstract::The Escherichia coli plasmids pBR322 and pBR327 can be taken up by Haemophilus influenzae but do not replicate in this organism; however, integration of pBR into the H. influenzae chromosome was achieved by ligation to a fragment of the Haemophilus phage S2 that carried a phage attachment site (attP). Once these seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.1898-1903.1989
更新日期:1989-04-01 00:00:00
abstract::Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.7.2279-2285.1999
更新日期:1999-04-01 00:00:00
abstract::Zorbamycin (U-30,604E) induces rapid degradation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in Bacillus subtilis cells. DNA degradation is initiated first and is closely followed by the degradation of RNA. No interaction between isolated DNA and zorbamycin is observed. Nucleic acid and protein syntheses...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.30-37.1971
更新日期:1971-10-01 00:00:00
abstract::In an attempt to isolate the superoxide dismutase (SOD) gene from the anaerobic sulfate-reducing bacterium Desulfoarculus baarsii, a DNA fragment was isolated which functionally complemented an Escherichia coli mutant (sodA sodB) deficient in cytoplasmic SODs. This region carries two open reading frames with sequences...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.6736-6742.1996
更新日期:1996-12-01 00:00:00
abstract::Fatty acid desaturation catalyzed by fatty acid desaturases requires molecular oxygen (O(2)). Saccharomyces cerevisiae cells derepress expression of OLE1 encoding Delta9 fatty acid desaturase under hypoxic conditions to allow more-efficient use of limited O(2). It has been proposed that aerobic conditions lead to repr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.2.745-751.2001
更新日期:2001-01-01 00:00:00
abstract::Certain gram-negative pathogens are known to control virulence gene expression through cell-cell communication via small diffusible signal molecules termed autoinducers. This intercellular signal transduction mechanism termed quorum sensing depends on the interaction of an N-acylhomoserine lactone (AHL) auto-inducer m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.9.3004-3012.1997
更新日期:1997-05-01 00:00:00
abstract::Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1144-1148.1977
更新日期:1977-06-01 00:00:00
abstract::rpoS, a gene that encodes an alternative sigma factor (also known as katF), is critical for the ability of Yersinia enterocolitica grown at 37 degrees C, but not at 26 degrees C, to survive diverse environmental insults such as high temperature, hydrogen peroxide, osmolarity, and low pH. However, a Y. enterocolitica r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.18.5370-5373.1995
更新日期:1995-09-01 00:00:00
abstract::The coding region of the alpha-amylase inhibitor (HaimII) gene from the producing strain Streptomyces griseosporeus YM-25 was localized on an 800-base-pair DNA segment. The nucleotide sequence of a 1,191-base-pair region including the HaimII gene was determined by the dideoxy-chain termination method. The nucleotide s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4451-4457.1988
更新日期:1988-10-01 00:00:00
abstract::The major surface-located protein in the outer membrane of Haemophilus influenzae type b (Hib) is porin, molecular mass, 38 kDa, 341 amino acids. To define precisely the molecular reactivities of nine mouse monoclonal antibodies (MAbs) against Hib porin, overlapping hexapeptides corresponding to the entire sequence of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.12.4007-4016.1992
更新日期:1992-06-01 00:00:00
abstract::Mutants of Escherichia coli unable to synthesize a functional pyruvate formate-lyase (PFL) are severely impaired in their capacity to grow by glucose fermentation. In a functional complementation assay designed to isolate the pfl gene from Clostridium butyricum, we fortuitously identified a gene that did not encode a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.14.3509-3516.1998
更新日期:1998-07-01 00:00:00
abstract::Pseudomonas sp. strain LB400 grows on biphenyl as the sole carbon and energy source. This organism also cooxidizes several chlorinated biphenyl congeners. Biphenyl dioxygenase activity in cell extract required addition of NAD(P)H as an electron donor for the conversion of biphenyl to cis-2,3-dihydroxy-2,3-dihydrobiphe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.2.395-400.1993
更新日期:1993-01-01 00:00:00
abstract::Whole cells of Bacillus subtilis transported d-alanine and l-alanine by two different systems. The high-affinity system (K(m) of 1 muM and V(max) of 0.6 to 0.8 nmol/min per mg of protein) was specific for the two stereoisomers of alanine. The low-affinity system (K(m) of 10 muM for l-alanine and 20 muM for d-alanine a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.3.1085-1092.1974
更新日期:1974-12-01 00:00:00
abstract::Members of the Streptococcus bovis group are important causes of endocarditis. However, factors associated with their pathogenicity, such as adhesins, remain uncharacterized. We recently demonstrated that endocarditis-derived Streptococcus gallolyticus subsp. gallolyticus isolates frequently adhere to extracellular ma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00909-09
更新日期:2009-11-01 00:00:00
abstract::The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00737-10
更新日期:2011-01-01 00:00:00
abstract::Arcobacter butzleri strain ED-1 is an exoelectrogenic epsilonproteobacterium isolated from the anode biofilm of a microbial fuel cell. Arcobacter sp. strain L dominates the liquid phase of the same fuel cell. Here we report the finished and annotated genome sequences of these organisms. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06084-11
更新日期:2011-11-01 00:00:00
abstract::In bacteria, the 4-amino-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine is synthesized from 5-aminoimidazole ribotide (AIR), a branch point metabolite of purine and thiamine biosynthesis. ThiC is a member of the radical S-adenosylmethionine (AdoMet) superfamily and catalyzes the complex chemical rearrangeme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01361-12
更新日期:2012-11-01 00:00:00
abstract::Transcriptional expression of the puc operon in Rhodobacter sphaeroides 2.4.1 is dependent on the partial pressure of oxygen. By using transcriptional fusions in trans of a promoterless fragment derived from the aminoglycoside-3'-phosphotransferase gene of Tn903 to puc operon-specific DNA containing a 629-bp 5' cis-ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.4.1158-1171.1992
更新日期:1992-02-01 00:00:00
abstract::Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.3.816-822.1979
更新日期:1979-06-01 00:00:00