Abstract:
:Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harboring Tn916 delta E (a derivative in which erm is substituted for tet) on the chromosome and Tn916 on a nonconjugative plasmid. When these strains were used as donors, the two transposons were able to transfer independently; however, they were found to transfer and become coestablished in the recipient up to 50% of the time. In contrast, cotransfer of a plasmid marker located outside the transposon occurred at a frequency of no greater than 0.5%. Separate experiments showed that mobilization of the nonconjugative plasmids pAM401 and pVA749 by chromosome-borne copies of Tn916 occurred only at low frequencies (generally less than 2% cotransfer). The data imply that the initiation of transposition of Tn916 results in a trans activation that is specific for homologous transposons present in the same cell.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Flannagan SE,Clewell DBdoi
10.1128/jb.173.22.7136-7141.1991keywords:
subject
Has Abstractpub_date
1991-11-01 00:00:00pages
7136-41issue
22eissn
0021-9193issn
1098-5530journal_volume
173pub_type
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更新日期:1975-07-01 00:00:00
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更新日期:1969-08-01 00:00:00
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更新日期:2005-08-01 00:00:00
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更新日期:1984-03-01 00:00:00
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更新日期:1996-09-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1965-11-01 00:00:00
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更新日期:1998-03-01 00:00:00
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更新日期:2020-11-02 00:00:00