Abstract:
:Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One example is the needle subunit protein. Electron micrographs indicate that chlamydiae possess needle filaments, and yet database searches fail to identify a SctF homologue. We used a bioinformatics approach to identify a likely needle subunit protein for Chlamydia. Experimental evidence indicates that this protein, designated CdsF, has properties consistent with it being the major needle subunit protein. CdsF is concentrated in the outer membrane of elementary bodies and is surface exposed as a component of an extracellular needle-like projection. During infection CdsF is detectable by indirect immunofluorescence in the inclusion membrane with a punctuate distribution adjacent to membrane-associated reticulate bodies. Biochemical cross-linking studies revealed that, like other SctF proteins, CdsF is able to polymerize into multisubunit complexes. Furthermore, we identified two chaperones for CdsF, termed CdsE and CdsG, which have many characteristics of the Pseudomonas spp. needle chaperones PscE and PscG, respectively. In aggregate, our data are consistent with CdsF representing at least one component of the extended Chlamydia T3SS injectisome. The identification of this secretion system component is essential for studies involving ectopic reconstitution of the Chlamydia T3SS. Moreover, we anticipate that CdsF could serve as an efficacious target for anti-Chlamydia neutralizing antibodies.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Betts HJ,Twiggs LE,Sal MS,Wyrick PB,Fields KAdoi
10.1128/JB.01671-07subject
Has Abstractpub_date
2008-03-01 00:00:00pages
1680-90issue
5eissn
0021-9193issn
1098-5530pii
JB.01671-07journal_volume
190pub_type
杂志文章abstract::Hottle, G. A. (Naval Biological Laboratory, University of California, Berkeley), and D. N. Wright. Growth and survival of Mycoplasma neurolyticum in liquid media. J. Bacteriol. 91:1834-1839. 1966.-Maximal growth of Mycoplasma neurolyticum (between 10(8) and 10(9) colony-forming units per ml) was obtained after 3 days ...
journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1985-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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更新日期:1977-10-01 00:00:00
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更新日期:1987-08-01 00:00:00
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更新日期:1969-03-01 00:00:00
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更新日期:2015-07-01 00:00:00
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更新日期:1967-11-01 00:00:00