Structural elements required for replication and incompatibility of the Rhizobium etli symbiotic plasmid.

abstract：:Integrons constitute a novel family of DNA elements which evolved by site-specific integration of discrete units between two conserved segments. On the In4 integron of Tn1696, a precisely inserted gene cassette of 1,549 bp conferring nonenzymatic chloramphenicol resistance (cmlA) is present between the streptomycin-sp...

journal_title：Journal of bacteriology

authors： Bissonnette L,Champetier S,Buisson JP,Roy PH

更新日期：1991-07-01 00:00:00

abstract：:The mechanism of methyl-beta-D-thiogalactoside-phosphate (TMG-P) expulsion from Streptococcus pyogenes was studied. The expulsion elicited by glucose was not due to exchange vectorial transphosphorylation between the expelled TMG and the incoming glucose since more beta-galactoside was displaced than glucose taken up,...

journal_title：Journal of bacteriology

authors： Reizer J,Novotny MJ,Panos C,Saier MH Jr

更新日期：1983-10-01 00:00:00

abstract：:Proteus mirabilis, a leading cause of catheter-associated urinary tract infection (CaUTI), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. While many genes and nutrient requirements involved in the swarming process have been identified, few studies have addre...

journal_title：Journal of bacteriology

authors： Armbruster CE,Hodges SA,Mobley HL

更新日期：2013-03-01 00:00:00

abstract：:The antibiotic d-cycloserine is an effective inhibitor of alanine racemase. The lack of inhibition by l-cycloserine of alanine racemase from Staphylococcus aureus led Roze and Strominger to formulate the cycloserine hypothesis. This hypothesis states that d-cycloserine has the conformation required of the substrates o...

journal_title：Journal of bacteriology

authors： Lambert MP,Neuhaus FC

更新日期：1972-06-01 00:00:00

abstract：:A Bacillus subtilis secA mutant, secA12, which is blocked at an early stage of sporulation, is able to grow as well as the wild-type strain at all temperatures tested. Experiments with lacZ fusion genes showed that the induction of kinA expression, as well as the sporulation-specific transcription of the spo0A gene, w...

journal_title：Journal of bacteriology

authors： Asai K,Kawamura F,Sadaie Y,Takahashi H

更新日期：1997-01-01 00:00:00

abstract：:Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to dis...

journal_title：Journal of bacteriology

authors： Kocourek J,Ballou CE

更新日期：1969-12-01 00:00:00

abstract：:Glyoxal is toxic and mutagenic α-oxoaldehyde generated in vivo as an oxidation by-product of sugar metabolism. We selected glyoxal-resistant mutants from an Escherichia coli strain lacking major glyoxal-detoxifying genes, gloA and yqhD, by growing cells in medium containing a lethal concentration of glyoxal. The mutan...

journal_title：Journal of bacteriology

authors： Kwon M,Lee J,Lee C,Park C

更新日期：2012-04-01 00:00:00

abstract：:ShdA is a large outer membrane protein of the autotransporter family whose passenger domain binds the extracellular matrix proteins fibronectin and collagen I, possibly by mimicking the host ligand heparin. The ShdA passenger domain consists of approximately 1,500 amino acid residues that can be divided into two regio...

journal_title：Journal of bacteriology

authors： Kingsley RA,Abi Ghanem D,Puebla-Osorio N,Keestra AM,Berghman L,Bäumler AJ

更新日期：2004-08-01 00:00:00

abstract：:A pattern of allosteric control for aromatic biosynthesis in cyanobacteria relies upon early-pathway regulation as the major control point for the entire branched pathway. In Synechococcus sp. strain PCC6301 (Anacystis nidulans), two enzymes which form precursors for L-phenylalanine biosynthesis are subject to control...

journal_title：Journal of bacteriology

authors： Hall GC,Flick MB,Jensen RA

更新日期：1983-01-01 00:00:00

abstract：:Mobilizable genomic islands (MGIs) are small genomic islands that are mobilizable by SXT/R391 integrating conjugative elements (ICEs) due to similar origins of transfer. Their site-specific integration and excision are catalyzed by the integrase that they encode, but their conjugative transfer entirely depends upon th...

journal_title：Journal of bacteriology

authors： Daccord A,Mursell M,Poulin-Laprade D,Burrus V

更新日期：2012-11-01 00:00:00

abstract：:"Roseburia inulinivorans" is an anaerobic polysaccharide-utilizing firmicute bacterium from the human colon that was identified as a producer of butyric acid during growth on glucose, starch, or inulin. R. inulinivorans A2-194 is also able to grow on the host-derived sugar fucose, following a lag period, producing pro...

journal_title：Journal of bacteriology

authors： Scott KP,Martin JC,Campbell G,Mayer CD,Flint HJ

更新日期：2006-06-01 00:00:00

abstract：:Bacterial lipases play important roles in bacterial metabolism and environmental response. Our laboratory recently discovered that a novel lipoprotein lysophospholipase, VolA, localizes on the surface of the Gram-negative aquatic pathogen Vibrio cholerae. VolA functions to cleave exogenous lysophosphatidylcholine, fre...

journal_title：Journal of bacteriology

authors： Pride AC,Guan Z,Trent MS

更新日期：2014-04-01 00:00:00

abstract：:Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunological techniques, we have compared the synthesis of the phoA protein (alkaline phosphatase) and the phoS protein (phosphate-binding protein) in response to the level of phosphate in the medium in different genetic backgrounds containing the kn...

journal_title：Journal of bacteriology

authors： Willsky GR,Malamy MH

更新日期：1976-07-01 00:00:00

abstract：:We showed that the heat killing curve for exponentially growing Saccharomyces cerevisiae was biphasic. This suggests two populations of cells with different thermal killing characteristics. When exponentially growing cells separated into cell cycle-specific fractions via centrifugal elutriation were heat shocked, the ...

journal_title：Journal of bacteriology

authors： Plesset J,Ludwig JR,Cox BS,McLaughlin CS

更新日期：1987-02-01 00:00:00

abstract：:The ability of Rickettsia prowazekii to transport potential sources of the glucose moiety of bacterial polysaccharides was determined. Transport was determined both by filtration assays and by centrifugation through nonaqueous layers. Uridine 5'-diphosphoglucose (UDPG) was transported, whereas glucose was not transpor...

journal_title：Journal of bacteriology

authors： Winkler HH,Daugherty RM

更新日期：1986-09-01 00:00:00

abstract：:Capsulated Haemophilus influenzae type b and two spontaneous mutants (classes I and II variants) were characterized by transmission and scanning electron microscopy. When cells were treated with type b-specific antiserum prior to manipulations for electron microscopy, sectioned capsulated cells had electron-dense, fib...

journal_title：Journal of bacteriology

authors： Doern GV,Buckmire FL

更新日期：1976-07-01 00:00:00

abstract：:The isolation and properties of a mutant dependent upon exogenous carbamyl aspartate for resistance to 5-fluorouracil are described. The mutant was deficient in the synthesis of ubiquinone and accumulated a quinone provisionally identified as the ubiquinone precursor 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. T...

journal_title：Journal of bacteriology

authors： Zak VL,Kelln RA

更新日期：1981-02-01 00:00:00

abstract：:Dehority, B. A. (Ohio Agricultural Experiment Station, Wooster). Degradation and utilization of isolated hemicellulose by pure cultures of cellulolytic rumen bacteria. J. Bacteriol. 89:1515-1520. 1965.-Hemicelluloses isolated from flax, corn hulls, alfalfa, oat hulls, and fescue grass were used as sole energy sources ...

journal_title：Journal of bacteriology

authors： DEHORITY BA

更新日期：1965-06-01 00:00:00

abstract：:Transcriptome analysis of a Bacillus subtilis rok strain that showed reduced complex colony structure formation revealed significant downregulation of the yuaB gene. Overexpression of yuaB restored structure formation in the rok strain. We show that transcription of yuaB is indirectly regulated by Rok, independently f...

journal_title：Journal of bacteriology

authors： Kovács AT,Kuipers OP

更新日期：2011-02-01 00:00:00

abstract：:Strains carrying either the polA1 or resA1 mutation are deficient in DNA polymerase I, and the polA1 and resA1 mutations do not complement in merozygotes. The effect of these mutations in otherwise identical genetic backgrounds was studied: after ultraviolet irradiation both strains degrade their DNA more rapidly and ...

journal_title：Journal of bacteriology

authors： Glickman BW,van Sluis CA,van der Maas G,Rörsch A

更新日期：1973-06-01 00:00:00

abstract：:A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but ...

journal_title：Journal of bacteriology

authors： Maniloff J,Quinlan DC

更新日期：1974-10-01 00:00:00

abstract：:The complete sequences of the RhsB and RhsC elements of Escherichia coli K-12 have been determined. These sequence data reveal a new repeated sequence, called H-rpt (Hinc repeat), which is distinct from the Rhs core repetition that is found in all five Rhs elements. H-rpt is found in RhsB, RhsC, and RhsE. Characteriza...

journal_title：Journal of bacteriology

authors： Zhao S,Sandt CH,Feulner G,Vlazny DA,Gray JA,Hill CW

更新日期：1993-05-01 00:00:00

abstract：:Allantoate uptake appears to be mediated by an energy-dependent active transport system with an apparent Michaelis constant of about 50 microM. Cells were able to accumulate allantoate to greater than 3,000 times the extracellular concentration. The rate of accumulation was maximum at pH 5.7 to 5.8. The energy source ...

journal_title：Journal of bacteriology

authors： Turoscy V,Cooper TG

更新日期：1979-12-01 00:00:00

abstract：:The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli...

journal_title：Journal of bacteriology

authors： Nelson DR,Rittenberg SC

更新日期：1981-09-01 00:00:00

abstract：:The methanol-insoluble, heat-stable enterotoxin of Escherichia coli synthesized by clinical strains or strains that harbor the cloned gene was shown to be an extracellular polypeptide. The toxin (STB) was first detected as an 8,100-Mr precursor (pre-STB) that was converted to a transiently cell-associated 5,200-Mr for...

journal_title：Journal of bacteriology

authors： Kupersztoch YM,Tachias K,Moomaw CR,Dreyfus LA,Urban R,Slaughter C,Whipp S

更新日期：1990-05-01 00:00:00

abstract：:There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to exami...

journal_title：Journal of bacteriology

authors： Nagarajan S,Sherman DM,Shaw I,Sherman LA

更新日期：2012-01-01 00:00:00

abstract：:The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of pr...

journal_title：Journal of bacteriology

authors： David S,van der Rest ME,Driessen AJ,Simons G,de Vos WM

更新日期：1990-10-01 00:00:00

abstract：:The gene encoding OprF, a major outer membrane protein in Pseudomonas species (formerly known as type 1 pseudomonads), was thought to be constitutively transcribed from a single sigma 70 promoter immediately upstream of the gene. We now report the identification of a novel putative ECF (extracytoplasmic function) sigm...

journal_title：Journal of bacteriology

authors： Brinkman FS,Schoofs G,Hancock RE,De Mot R

更新日期：1999-08-01 00:00:00

abstract：:SufA, IscA, and Nfu have been proposed to function as scaffolds in the assembly of Fe/S clusters in bacteria. To investigate the roles of these proteins further, single and double null-mutant strains of Synechococcus sp. strain PCC 7002 were constructed by insertional inactivation of genes homologous to sufA, iscA, an...

journal_title：Journal of bacteriology

authors： Balasubramanian R,Shen G,Bryant DA,Golbeck JH

更新日期：2006-05-01 00:00:00

abstract：:During conjugation, a single strand of DNA is cleaved at the origin of transfer (oriT) by the plasmid-encoded relaxase. This strand is then unwound from its complement and transferred in the 5'-to-3' direction, with the 3' end likely extended by rolling-circle replication. The resulting, newly synthesized oriT must th...

journal_title：Journal of bacteriology

authors： Becker EC,Meyer R

更新日期：2012-10-01 00:00:00