Abstract:
:Listeria monocytogenes is a model facultative intracellular pathogen. Tight regulation of virulence proteins is essential for a successful infection, and the gene encoding the annotated thioredoxin YjbH was identified in two forward genetic screens as required for virulence factor production. Accordingly, an L. monocytogenes strain lacking yjbH is attenuated in a murine model of infection. However, the function of YjbH in L. monocytogenes has not been investigated. Here, we provide evidence that L. monocytogenes YjbH is involved in the nitrosative stress response, likely through its interaction with the redox-responsive transcriptional regulator SpxA1. YjbH physically interacted with SpxA1, and our data support a model in which YjbH is a protease adaptor that regulates SpxA1 protein abundance. Whole-cell proteomics identified eight additional proteins whose abundance was altered by YjbH, and we demonstrated that YjbH physically interacted with each in bacterial two-hybrid assays. Thioredoxin proteins canonically require active motif cysteines for function, but thioredoxin activity has not been tested for L. monocytogenes YjbH. We demonstrated that cysteine residues of the YjbH thioredoxin domain active motif are essential for L. monocytogenes sensitivity to nitrosative stress, cell-to-cell spread in a tissue culture model of infection, and several protein-protein interactions. Together, these results demonstrated that the function of YjbH in L. monocytogenes requires its thioredoxin active motif and that YjbH has a role in the posttranslational regulation of several proteins, including SpxA1.IMPORTANCE The annotated thioredoxin YjbH in Listeria monocytogenes has been implicated in virulence, but its function in the cell is unknown. In other bacterial species, YjbH is a protease adaptor that mediates degradation of the transcriptional regulator Spx. Here, we investigated the function of L. monocytogenes YjbH and demonstrated its role in the nitrosative stress response and posttranslational regulation of several proteins with which YjbH physically interacts, including SpxA1. Furthermore, we demonstrated that the cysteine residues of the YjbH thioredoxin active motif are required for the nitrosative stress response, cell-to-cell spread, and some protein-protein interactions. YjbH is widely conserved among Firmicutes, and this work reveals its unique requirement of the thioredoxin-active motif in L. monocytogenes.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ruhland BR,Reniere MLdoi
10.1128/JB.00099-20subject
Has Abstractpub_date
2020-05-27 00:00:00issue
12eissn
0021-9193issn
1098-5530pii
JB.00099-20journal_volume
202pub_type
杂志文章abstract::Isolation of highly purified membrane fractions from phototrophically grown Rhodospirillum rubrum was achieved by velocity and isopyknic sedimentation under carefully controlled ionic conditions. Bacteriochlorophyll-rich and succinic dehydrogenase-rich chromatophores that were essentially devoid of contamination by no...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.3.1326-1338.1976
更新日期:1976-06-01 00:00:00
abstract::The effect of the ilvG671, ilvG468, and ilvG603 mutations (phenotype, IlvG+ Valr; formerly ilvO) upon proteins synthesized was determined by infection of irradiated Escherichia coli K-12 cells, using specifically constructed derivatives of lambda dilv phage. These ilvG alleles are similar to the previously studied ilv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.127-134.1982
更新日期:1982-07-01 00:00:00
abstract::Movement on surfaces, or swarming motility, is effectively mediated by the lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf is induced by conditions inhibiting rotation of the polar flagellum, which is used for swimming in liquid. However, not all V. parahaemolyticus isolates swarm proficie...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.7.2625-2635.2006
更新日期:2006-04-01 00:00:00
abstract::This study describes a novel approach to identify unique genomic DNA sequences from the unsequenced strain C. jejuni ATCC 43431 by comparison with the sequenced strain C. jejuni NCTC 11168. A shotgun DNA microarray was constructed by arraying 9,600 individual DNA fragments from a C. jejuni ATCC 43431 genomic library o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.14.4781-4795.2004
更新日期:2004-07-01 00:00:00
abstract::A sequence-specific modification methylase (M . SinI) was isolated and purified from Escherichia coli harboring a derivative of recombinant plasmid pSI4 (see accompanying manuscript: C. Karreman and A. de Waard, J. Bacteriol. 170:2527-2532, 1988), which contains a Salmonella infantis DNA insert. The enzyme uniquely me...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2533-2536.1988
更新日期:1988-06-01 00:00:00
abstract::Photorhabdus is a genus of gram-negative Enterobacteriaceae that is pathogenic to insect larvae while also maintaining a mutualistic relationship with nematodes from the family Heterorhabditis, where the bacteria occupy the gut of the infective juvenile (IJ) stage of the nematode. In this study we describe the identif...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.1.77-84.2005
更新日期:2005-01-01 00:00:00
abstract::A Rhodobacter sphaeroides mutant (CYCA1) lacking cytochrome c2 (cyt c2) was previously constructed (T. J. Donohue, A. G. McEwan, S. Van Doren, A. R. Crofts, and S. Kaplan, Biochemistry, 27: 1918-1924, 1988) by a combination of in vivo and in vitro molecular genetic techniques. CYCA1 was incapable of photosynthetic gro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.360-368.1989
更新日期:1989-01-01 00:00:00
abstract::The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.10.2892-2897.1994
更新日期:1994-05-01 00:00:00
abstract::The Escherichia coli promoter p(BAD), under the control of the AraC protein, drives the expression of mRNA encoding the AraB, AraA, and AraD gene products of the arabinose operon. The binding site of AraC at p(BAD) overlaps the RNA polymerase -35 recognition region by 4 bases, leaving 2 bases of the region not contact...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.18.5076-5081.2000
更新日期:2000-09-01 00:00:00
abstract::Mycoplasma fermentans is a microorganism commonly found in the genitourinary and respiratory tracts of healthy individuals and AIDS patients. The complete genome of the repetitive-sequence-rich M. fermentans strain M64 is reported here. Comparative genomics analysis revealed dramatic differences in genome size between...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05228-11
更新日期:2011-08-01 00:00:00
abstract::The site of integration and the molecular orientation of a prophage Mu integrated within the resistance transfer factor component of plasmid R100-1 have been determined on the physical map of the plasmid. This allowed us (i) to determine the direction of deoxyribonucleic acid transfer from oriT during conjugation and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.1105-1108.1979
更新日期:1979-12-01 00:00:00
abstract::A homologue of the grmA spore germination gene of Bacillus megaterium and of a NaH-antiporter gene (napA) of Enterococcus hirae has been identified in Bacillus cereus 569 (ATCC 10876). The putative protein product has 58 and 43% amino acid identity with GrmA and NapA, respectively. Insertional inactivation of this B. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.2.476-482.2001
更新日期:2001-01-01 00:00:00
abstract::A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variation...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.3.1162-1165.1985
更新日期:1985-06-01 00:00:00
abstract::The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.5.1560-1566.1968
更新日期:1968-05-01 00:00:00
abstract::Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.3.784-794.1974
更新日期:1974-09-01 00:00:00
abstract::Achromobacter xylosoxidans strain A8 was isolated from soil contaminated with polychlorinated biphenyls. It can use 2-chlorobenzoate and 2,5-dichlorobenzoate as sole sources of carbon and energy. This property makes it a good starting microorganism for further development toward a bioremediation tool. The genome of A....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01299-10
更新日期:2011-02-01 00:00:00
abstract::Streptomyces venezuelae ATCC 10595 harbors seven rRNA gene clusters which can be distinguished by BglII digestion. The three rRNA genes present in each set are closely linked with the general structure 16S-23S-5S. We cloned rrnA and sequenced the 16S-23S spacer region and the region downstream of the 5S rRNA gene. No ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.5.1480-1483.1996
更新日期:1996-03-01 00:00:00
abstract::When the crude ribosomal fraction of Saccharomyces cerevisiae was separated into "light" and "heavy" fractions, fatty acid synthetase was concentrated in the former, whereas acetyl-Coenzyme A synthetase, fatty acid "desaturase," and squalene oxidocyclase were found in the latter. The "desaturase" sedimented with the r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.61-65.1967
更新日期:1967-07-01 00:00:00
abstract::ShdA is a large outer membrane protein of the autotransporter family whose passenger domain binds the extracellular matrix proteins fibronectin and collagen I, possibly by mimicking the host ligand heparin. The ShdA passenger domain consists of approximately 1,500 amino acid residues that can be divided into two regio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.15.4931-4939.2004
更新日期:2004-08-01 00:00:00
abstract::Candida albicans produces large amounts of the acyclic pentitol D-arabitol in culture and in infected animals and humans, and most strains also grow on minimal D-arabitol medium. An earlier study showed that the major metabolic precursor of D-arabitol in C. albicans was D-ribulose-5-PO4 from the pentose pathway, that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.11.2971-2976.1995
更新日期:1995-06-01 00:00:00
abstract::Two mutations in the ninR region of bacteriophage lambda that bypass a requirement for antitermination have been studied. One mutation, byp, has been cloned and mapped by marker rescue to a 417-base-pair segment in the ninR region of the genome. Analysis of the byp mutation by using promoter detection vectors, DNA seq...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.8.4610-4615.1990
更新日期:1990-08-01 00:00:00
abstract::The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich regio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.14.4520-4527.1993
更新日期:1993-07-01 00:00:00
abstract::Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydroly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
abstract::The bacterium Myxococcus xanthus undergoes multicellular development during times of nutritional stress and uses extracellular signals to coordinate cell behavior. C-signal affects gene expression late in development, including that of Omega4499, an operon identified by insertion of Tn5 lac into the M. xanthus chromos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.12.3766-3776.2004
更新日期:2004-06-01 00:00:00
abstract::Penicillin-binding proteins were identified in Borrelia burgdorferi membranes. A 94-kilodalton penicillin-binding protein was the first to be labeled with tritiated penicillin and was the first band to disappear in a competition experiment. Its binding ability was destroyed when membranes were preboiled. In addition, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.6139-6141.1990
更新日期:1990-10-01 00:00:00
abstract::Saz, Arthur K. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Dolores L. Lowery, and Leah J. Jackson. Staphylococcal penicillinase. I. Inhibition and stimulation of activity. J. Bacteriol. 82:298-304. 1961.-The penicillinase extracted from a penicillin-resistant strain of Staphylococcus aureus...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.2.298-304.1961
更新日期:1961-08-01 00:00:00
abstract::A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complement...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.19.6058-6065.1991
更新日期:1991-10-01 00:00:00
abstract::The twisting and writhing during growth of single-cell filaments of Bacillus subtilis which lead to macrofiber formation was studied in both left- and right-handed forms of strains FJ7 and RHX. Filament bending, touching, and loop formation (folding), followed by winding up into a double-strand fiber, were documented....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7060-7069.1995
更新日期:1995-12-01 00:00:00
abstract::The proteome of Haemophilus influenzae strain Rd KW20 was analyzed by liquid chromatography (LC) coupled with ion trap tandem mass spectrometry (MS/MS). This approach does not require a gel electrophoresis step and provides a rapidly developed snapshot of the proteome. In order to gain insight into the central metabol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.15.4593-4602.2003
更新日期:2003-08-01 00:00:00
abstract::In Escherichia coli, nine essential cell division proteins are known to localize to the division septum. FtsL is a 13-kDa bitopic membrane protein with a short cytoplasmic N-terminal domain, a membrane-spanning segment, and a periplasmic domain that has a repeated heptad motif characteristic of leucine zippers. Here, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.1.116-129.2000
更新日期:2000-01-01 00:00:00