Rabbit ileal loop response to strains of Clostridium perfringens.

Abstract:

:The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C in Skim Milk (Difco), 14 of 29 type A strains isolated from food-poisoning outbreaks consistently produced exudation of fluid and consequent dilation of the ileal segments. In contrast, 15 of the 18 strains derived from other sources failed to elicit a response. By use of different inoculum preparations, nearly all strains could be made to give at least an occasional positive loop reaction. Diarrhea was not obtained in rabbits by intraluminal injection into the normal ileum or by per os administration of the cultures. Lecithinase, purified and in concentrated culture supernatant fractions, failed to produce a response in the isolated ileal loops.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Duncan CL,Sugiyama H,Strong DH

doi

10.1128/JB.95.5.1560-1566.1968

subject

Has Abstract

pub_date

1968-05-01 00:00:00

pages

1560-6

issue

5

eissn

0021-9193

issn

1098-5530

journal_volume

95

pub_type

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