Abstract:
:A colorimetric assay was developed for studying the kinetics of iron oxidation with whole cells of the chemoautotroph, Ferrobacillus ferrooxidans. The assay was more advantageous than the conventional method of Warburg manometry because of its simplicity, rapidity, and the small amount of cells required. The assay measured Fe(3+) as a chloride complex which absorbs at 410 nm. Kinetic analysis showed the apparent K(m) for iron oxidation to be 5.4 x 10(-3)m in an unbuffered system and 2.2 x 10(-3)m in the presence of beta-alanine-SO(4) (2-) buffer. Glycine and beta-alanine buffers were used in the measurement of the pH optimum for iron oxidation; the optimum ranged from 2.5 to 3.8. The effect of pH was primarily on the V(max) while the K(m) remained constant. Added SO(4) (2-) was found to stimulate iron oxidation by increasing the V(max) of iron oxidation by whole cells, but it did not affect the K(m). Results of assays of iron oxidation in systems containing various mole percentages of SO(4) (2-) and Cl(-) indicated that Cl(-) did not inhibit iron oxidation but that SO(4) (2-) was required. Sulfate could be partially replaced by HPO(4) (2-) and HAsO(4) (2-) but not by BO(3) (-), MoO(4) (2-), NO(3) (-), or Cl(-); formate and MoO(4) (2-) inhibited iron oxidation.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Schnaitman CA,Korczynski MS,Lundgren DGdoi
10.1128/JB.99.2.552-557.1969subject
Has Abstractpub_date
1969-08-01 00:00:00pages
552-7issue
2eissn
0021-9193issn
1098-5530journal_volume
99pub_type
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journal_title:Journal of bacteriology
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更新日期:2017-08-08 00:00:00
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更新日期:1973-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
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doi:10.1128/JB.85.2.279-283.1963
更新日期:1963-02-01 00:00:00
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更新日期:1968-05-01 00:00:00
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更新日期:1982-07-01 00:00:00
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更新日期:2013-03-01 00:00:00
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更新日期:1981-01-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1969-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1975-07-01 00:00:00
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doi:10.1128/JB.105.1.396-407.1971
更新日期:1971-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.900-910.1978
更新日期:1978-09-01 00:00:00
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更新日期:1985-05-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.181.3.869-878.1999
更新日期:1999-02-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.184.10.2709-2718.2002
更新日期:2002-05-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2020-11-19 00:00:00
abstract::The current work by Jain et al. (S. Jain, A. M. Chang, M. Singh, J. S. McLean, et al., J Bacteriol 201:e00683-18, 2019, https://doi.org/10.1128/JB.00683-18) reports the cloning of the lipid A deacylase gene of Porphyromonas gingivalis and the phenotypic characterization of the enzyme. Attempts to clone the gene and th...
journal_title:Journal of bacteriology
pub_type: 评论,杂志文章
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更新日期:2019-05-08 00:00:00
abstract::The 5,10-methylenetetrahydrofolate dehydrogenase of heterotrophically grown Peptostreptococcus productus Marburg was purified to apparent homogeneity. The purified enzyme catalyzed the reversible oxidation of methylenetetrahydrofolate with NADP+ as the electron acceptor at a specific activity of 627 U/mg of protein. T...
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pub_type: 杂志文章
doi:10.1128/jb.173.4.1414-1419.1991
更新日期:1991-02-01 00:00:00