The Saccharomyces cerevisiae mevalonate diphosphate decarboxylase is essential for viability, and a single Leu-to-Pro mutation in a conserved sequence leads to thermosensitivity.

abstract：:Rhodobacter sphaeroides maintained intracellular ammonium pools of 1.1 to 2.6 mM during growth in several fixed nitrogen sources as well as during diazotrophic growth. Addition of 0.15 mM NH4+ to washed, nitrogen-free cell suspensions was followed by linear uptake of NH4+ from the medium and transient formation of int...

journal_title：Journal of bacteriology

authors： Cordts ML,Gibson J

更新日期：1987-04-01 00:00:00

abstract：:Yersinia pestis, which causes bubonic and pneumonic plague, forms pigmented red colonies on Congo red (CR) dye agar. The hmsHFRS genes required for CR binding (Crb(+)) are genetically linked to virulence-associated genes encoding a siderophore uptake system. These genes are contained in a 102-kb chromosomal pgm locus ...

journal_title：Journal of bacteriology

authors： Hare JM,McDonough KA

更新日期：1999-08-01 00:00:00

abstract：:Aspartate transcarbamylase is synthesized during exponential growth of Bacillus subtilis and is inactivated when the cells enter the stationary phase. This work is a study of the regulation of aspartate transcarbamylase synthesis during growth and the stationary phase. Using specific immunoprecipitation of aspartate t...

journal_title：Journal of bacteriology

authors： Maurizi MR,Switzer RL

更新日期：1978-09-01 00:00:00

abstract：:Antiserum prepared against protocatechuate 3, 4-dioxygenase from Pseudomonas aeruginosa forms precipitin bands without spurs with isofunctional enzymes from different strains of the fluorescent pseudomonads on immunodiffusion plates. Catalytic activity of the isofunctional enzymes was inhibited by an immunoglobulin fr...

journal_title：Journal of bacteriology

authors： Hou CT,Lillard MO

更新日期：1976-04-01 00:00:00

abstract：:The pbpB gene product penicillin-binding protein 3 (PBP3) of Escherichia coli is one of the major targets of beta-lactam antibiotics. At the permissive temperature, the temperature-sensitive pbpBr1 mutant, which was obtained after selection for increased resistance to cephalexin, shows a dramatic change in shape which...

journal_title：Journal of bacteriology

authors： Taschner PE,Ypenburg N,Spratt BG,Woldringh CL

更新日期：1988-10-01 00:00:00

abstract：:In vitro complementation assays have been used to demonstrate the induction of alkane hydroxylase proteins in mutants lacking the ability to convert n-alkanes to their primary alcohols. Purified heptane is an effective inducer in a mutant lacking detectable hydroxylase activity. ...

journal_title：Journal of bacteriology

authors： Benson S,Shapiro J

更新日期：1975-08-01 00:00:00

abstract：:We cloned the cryptic phn operon of a K-12 strain, phn(EcoK), and analyzed the nucleotide sequence of the phn region (11,672 bp). An mRNA start site upstream of the phnC gene was identified by S1 nuclease mapping. The pho regulon activator PhoB protects a pho box region near the mRNA start in DNase I footprinting and ...

journal_title：Journal of bacteriology

authors： Makino K,Kim SK,Shinagawa H,Amemura M,Nakata A

更新日期：1991-04-01 00:00:00

abstract：:A fragment of the Lactococcus lactis chromosome containing an open reading frame of 265 codons, denoted bglR, has been characterized. The polypeptide encoded by bglR shares 36 to 30% sequence identity with a family of regulatory proteins including ArbG from Erwinia chrysanthemi, BglG from Escherichia coli, and SacT an...

journal_title：Journal of bacteriology

authors： Bardowski J,Ehrlich SD,Chopin A

更新日期：1994-09-01 00:00:00

abstract：:A 3,373-base-pair DNA segment from a clone fortuitously isolated from Saccharopolyspora erythraea by hybridization to an oligodeoxynucleotide probe was sequenced. Computer-assisted analysis of the nucleotide sequence reveals three closely linked Streptomyces open reading frames plus a fourth converging on the others. ...

journal_title：Journal of bacteriology

authors： Donadio S,Shafiee A,Hutchinson CR

更新日期：1990-01-01 00:00:00

abstract：:Eight metH mutants in Salmonella typhimurium with closely linked sites of mutation which could grow only on methionine were isolated from a metE mutant deficient in N(5)-methyltetrahydropteroyltriglutamate-homocysteine transmethylase; their deficiency in cobalamin-dependent N(5)-methyltetrahydrofolate-homocysteine tra...

journal_title：Journal of bacteriology

authors： Childs JD,Smith DA

更新日期：1969-10-01 00:00:00

abstract：:Bacteroides fragilis synthesizes eight distinct capsular polysaccharides, more than any described bacterium outside the order Bacteroidales. Here, we show that this organism also produces a high-molecular-weight extracellular polysaccharide (EPS). Expression of the EPS results in the formation of a large polysaccharid...

journal_title：Journal of bacteriology

authors： Chatzidaki-Livanis M,Coyne MJ,Roche-Hakansson H,Comstock LE

更新日期：2008-02-01 00:00:00

abstract：:Wallace, W. R. (Louisiana State University, Baton Rouge), and G. T. Dimopoullos. Catalase activity in Anaplasma marginale. J. Bacteriol. 91:309-311. 1965.-Extracts of erythrocytes infected with Anaplasma marginale were found to contain more catalase activity than normal erythrocytic preparations. The increase in catal...

journal_title：Journal of bacteriology

authors： WALLACE WR,DIMOPOULLOS GT

更新日期：1965-08-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena sp. strain PCC 7120 can fix N(2) under oxic conditions, and the activity of nitrogen fixation occurs exclusively in heterocysts, cells differentiated from vegetative cells in response to a limitation of a combined-nitrogen source in the growth medium. At the late stages of heter...

journal_title：Journal of bacteriology

authors： Shi L,Li JH,Cheng Y,Wang L,Chen WL,Zhang CC

更新日期：2007-07-01 00:00:00

abstract：:Certain oral treponemes express a highly proteolytic phenotype and have been associated with periodontal diseases. The periodontal pathogen Treponema denticola produces dentilisin, a serine protease of the subtilisin family. The two-gene operon prcA-prtP is required for expression of active dentilisin (PrtP), a putati...

journal_title：Journal of bacteriology

authors： Correia FF,Plummer AR,Ellen RP,Wyss C,Boches SK,Galvin JL,Paster BJ,Dewhirst FE

更新日期：2003-12-01 00:00:00

abstract：:To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...

journal_title：Journal of bacteriology

authors： Shaw GC,Sung CC,Liu CH,Kao HS

更新日期：1997-01-01 00:00:00

abstract：:The dnaA operon of Escherichia coli contains the genes dnaA, dnaN, and recF encoding DnaA, beta clamp of DNA polymerase III holoenzyme, and RecF. When the DnaA concentration is raised, an increase in the number of DNA replication initiation events but a reduction in replication fork velocity occurs. Because DnaA is au...

journal_title：Journal of bacteriology

authors： Grigorian AV,Lustig RB,Guzmán EC,Mahaffy JM,Zyskind JW

更新日期：2003-01-01 00:00:00

abstract：:The smallest viable cell of Spiroplasma citri is a two-turn helix (elementary helix). This elementary helix grows into longer parental cells, which then divide by constriction. The helical morphology is conserved during this process. The growth pattern of S. citri membranes has been investigated by different methods o...

journal_title：Journal of bacteriology

authors： Garnier M,Clerc M,Bové JM

更新日期：1984-04-01 00:00:00

abstract：:An unusual xylose isomerase produced by Thermoanaerobacterium strain JW/SL-YS 489 was purified 28-fold to gel electrophoretic homogeneity, and the biochemical properties were determined. Its pH optimum distinguishes this enzyme from all other previously described xylose isomerases. The purified enzyme had maximal acti...

journal_title：Journal of bacteriology

authors： Liu SY,Wiegel J,Gherardini FC

更新日期：1996-10-01 00:00:00

abstract：:CheY is the response regulator in the signal transduction pathway of bacterial chemotaxis. Position 106 of CheY is occupied by a conserved aromatic residue (tyrosine or phenylalanine) in the response regulator superfamily. A number of substitutions at position 106 have been made and characterized by both behavioral an...

journal_title：Journal of bacteriology

authors： Zhu X,Amsler CD,Volz K,Matsumura P

更新日期：1996-07-01 00:00:00

abstract：:The N-terminal sequence of a protein, originally described as an adhesin of Helicobacter pylori, was used in an oligonucleotide-based screening procedure of an H. pylori plasmid library in Escherichia coli. Five independent plasmid clones were isolated, all mapping to the same chromosomal region and encoding the H. py...

journal_title：Journal of bacteriology

authors： Odenbreit S,Wieland B,Haas R

更新日期：1996-12-01 00:00:00

abstract：:Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. IV. Active transducing phage P1 CM produced by the combination of R factor with phage P1. J. Bacteriol. 88:1266-1276. 1964.-During an investigation of the transduction of R factors with phage P1, a phage lysate...

journal_title：Journal of bacteriology

authors： KONDO E,MITSUHASHI S

更新日期：1964-11-01 00:00:00

abstract：:CsgA mutants of Myxococcus xanthus appear to be defective in producing an extracellular molecule essential for the developmental behaviors of this bacterium. The csgA gene encodes a 17.7-kilodalton polypeptide whose function and cellular location were investigated with immunological probes. Large quantities of the Csg...

journal_title：Journal of bacteriology

authors： Shimkets LJ,Rafiee H

更新日期：1990-09-01 00:00:00

abstract：:The diversity of regulatory systems encoded by bacteria provides an indication of the variety of stresses and interactions that these organisms encounter in nature. We have been investigating how the plant pathogen Pseudomonas syringae pv. tomato DC3000 responds to iron limitation and have focused on the iron starvati...

journal_title：Journal of bacteriology

authors： Markel E,Maciak C,Butcher BG,Myers CR,Stodghill P,Bao Z,Cartinhour S,Swingle B

更新日期：2011-10-01 00:00:00

abstract：:Integral membrane proteins from over 20 ubiquitous families of channels, secondary carriers, and primary active transporters were analyzed for average size differences between homologues from the three domains of life: Bacteria, Archaea, and Eucarya. The results showed that while eucaryotic homologues are consistently...

journal_title：Journal of bacteriology

authors： Chung YJ,Krueger C,Metzgar D,Saier MH Jr

更新日期：2001-02-01 00:00:00

abstract：:The Escherichia coli promoter p(BAD), under the control of the AraC protein, drives the expression of mRNA encoding the AraB, AraA, and AraD gene products of the arabinose operon. The binding site of AraC at p(BAD) overlaps the RNA polymerase -35 recognition region by 4 bases, leaving 2 bases of the region not contact...

journal_title：Journal of bacteriology

authors： Dhiman A,Schleif R

更新日期：2000-09-01 00:00:00

abstract：:At specific times in the cell cycle, the bacterium Caulobacter crescentus assembles two major polar organelles, the flagellum and the stalk. Previous studies have shown that flbT mutants overproduce flagellins and are unable to form chemotaxis swarm rings. In this paper, we report alterations in both the stalk and the...

journal_title：Journal of bacteriology

authors： Driks A,Schoenlein PV,DeRosier DJ,Shapiro L,Ely B

更新日期：1990-04-01 00:00:00

abstract：:The chromosomal DNA replication origins (oriC) from two members of the family Enterobacteriaceae, Enterobacter aerogenes and Klebsiella pneumoniae, have been isolated as functional replication origins in Escherichia coli. The origins in the SalI restriction fragments of 17.5 and 10.2 kilobase pairs, cloned from E. aer...

journal_title：Journal of bacteriology

authors： Harding NE,Cleary JM,Smith DW,Michon JJ,Brusilow WS,Zyskind JW

更新日期：1982-12-01 00:00:00

abstract：:Band 7 proteins, which encompass members of the stomatin, prohibitin, flotillin, and HflK/C protein families, are integral membrane proteins that play important physiological roles in eukaryotes but are poorly characterized in bacteria. We have studied the band 7 proteins encoded by the cyanobacterium Synechocystis sp...

journal_title：Journal of bacteriology

authors： Boehm M,Nield J,Zhang P,Aro EM,Komenda J,Nixon PJ

更新日期：2009-10-01 00:00:00

abstract：:Extracellular secretion of Serratia marcescens nuclease occurs as a two-step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its ex...

journal_title：Journal of bacteriology

authors： Suh Y,Benedik MJ

更新日期：1997-02-01 00:00:00

abstract：:Shiga toxin (STX), a bacterial toxin produced by Shigella dysenteriae type 1, is a hexamer composed of five receptor-binding B subunits which encircle an alpha-helix at the carboxyl terminus of the enzymatic A polypeptide. Hybrid toxins constructed by fusing the A polypeptide sequences of STX and Shiga-like toxin type...

journal_title：Journal of bacteriology

authors： Jemal C,Haddad JE,Begum D,Jackson MP

更新日期：1995-06-01 00:00:00