Abstract:
:Band 7 proteins, which encompass members of the stomatin, prohibitin, flotillin, and HflK/C protein families, are integral membrane proteins that play important physiological roles in eukaryotes but are poorly characterized in bacteria. We have studied the band 7 proteins encoded by the cyanobacterium Synechocystis sp. strain PCC 6803, with emphasis on their structure and proposed role in the assembly and maintenance of the photosynthetic apparatus. Mutagenesis revealed that none of the five band 7 proteins (Slr1106, Slr1128, Slr1768, Sll0815, and Sll1021) was essential for growth under a range of conditions (including high light, salt, oxidative, and temperature stresses), although motility was compromised in an Slr1768 inactivation mutant. Accumulation of the major photosynthetic complexes in the thylakoid membrane and repair of the photosystem II complex following light damage were similar in the wild type and a quadruple mutant. Cellular fractionation experiments indicated that three of the band 7 proteins (Slr1106, Slr1768, and Slr1128) were associated with the cytoplasmic membrane, whereas Slr1106, a prohibitin homologue, was also found in the thylakoid membrane fraction. Blue native gel electrophoresis indicated that these three proteins, plus Sll0815, formed large (>669-kDa) independent complexes. Slr1128, a stomatin homologue, has a ring-like structure with an approximate diameter of 16 nm when visualized by negative stain electron microscopy. No evidence for band 7/FtsH supercomplexes was found. Overall, our results indicate that the band 7 proteins form large homo-oligomeric complexes but do not play a crucial role in the biogenesis of the photosynthetic apparatus in Synechocystis sp. strain PCC 6803.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Boehm M,Nield J,Zhang P,Aro EM,Komenda J,Nixon PJdoi
10.1128/JB.00644-09subject
Has Abstractpub_date
2009-10-01 00:00:00pages
6425-35issue
20eissn
0021-9193issn
1098-5530pii
JB.00644-09journal_volume
191pub_type
杂志文章abstract::Gough, Michael (Brown University, Providence, R.I.), and Seymour Lederberg. Methylated bases in the host-modified deoxyribonucleic acid of Escherichia coli and bacteriophage lambda. J. Bacteriol. 91:1460-1468. 1966.-The deoxyribonucleic acid (DNA) from strains of Escherichia coli and phage lambda was examined to deter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.4.1460-1468.1966
更新日期:1966-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.23.6866-6873.1995
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pub_type: 杂志文章
doi:10.1128/JB.181.24.7449-7456.1999
更新日期:1999-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01554-14
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.11.3318-3333.1991
更新日期:1991-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.15.4508-4518.2003
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.2.755-763.1984
更新日期:1984-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.3.1141-1156.1978
更新日期:1978-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.96.5.1690-1695.1968
更新日期:1968-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.273-277.1967
更新日期:1967-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.551-561.1984
更新日期:1984-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.2.649-655.1983
更新日期:1983-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.1126-1135.1975
更新日期:1975-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.183.15.4468-4476.2001
更新日期:2001-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2001-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1986-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.116.3.1160-1169.1973
更新日期:1973-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JB.128.1.80-85.1976
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pub_type: 杂志文章
doi:10.1128/jb.174.12.4064-4069.1992
更新日期:1992-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.18.5961-5970.1992
更新日期:1992-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.1163-1164.1984
更新日期:1984-06-01 00:00:00
abstract::The genome of Halobacterium sp. strain NRC-1 contains a large gene cluster, gvpMLKJIHGFEDACNO, that is both necessary and sufficient for the production of buoyant gas-filled vesicles. Due to the resistance of gas vesicles to solubilization, only the major gas vesicle protein GvpA and a single minor protein, GvpC, were...
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pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00